Restriction map for pF1KE1706
 (Linear) (Six-base) MAPSORT of: KIEE1706.seq  Check: 7232  from: 1  to: 633



KIEE1706.orf

 With 182 enzymes: SgfI * 

                        June 19, 2019 14:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      48     185     236     633 
   Size:         48     137      51     397

  Fragments arranged by size: 

                 397     137      51      48
 
AlwNI CAG_nnn'CTG

Cuts at:      0     237     633 
   Size:        237     396
 
ApoI r'AATT_y

Cuts at:      0     295     556     633 
   Size:        295     261      77

  Fragments arranged by size: 

                 295     261      77
 
BamHI G'GATC_C

Cuts at:      0     585     633 
   Size:        585      48
 
BanI G'GyrC_C

Cuts at:      0     259     532     633 
   Size:        259     273     101

  Fragments arranged by size: 

                 273     259     101
 
BbeI G_GCGC'C

Cuts at:      0     536     633 
   Size:        536      97
 
BfrBI ATG'CAT

Cuts at:      0     410     633 
   Size:        410     223
 
BglI GCCn_nnn'nGGC

Cuts at:      0      44     218     633 
   Size:         44     174     415

  Fragments arranged by size: 

                 415     174      44
 
Bme1580I G_kGCm'C

Cuts at:      0     262     633 
   Size:        262     371
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     633 
   Size:         61     572
 
Bpu10I CC'TnA_GC

Cuts at:      0       9     633 
   Size:          9     624
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     352     633 
   Size:        352     281
 
BsaHI Gr'CG_yC

Cuts at:      0     533     633 
   Size:        533     100
 
BseYI C'CCAG_C

Cuts at:      0     108     633 
   Size:        108     525
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     274     633 
   Size:        163     111     359

  Fragments arranged by size: 

                 359     163     111
 
BsiEI CG_ry'CG

Cuts at:      0     100     633 
   Size:        100     533
 
BsmI GAATG_Cn'

Cuts at:      0     412     633 
   Size:        412     221
 
Bsp1286I G_dGCh'C

Cuts at:      0     262     633 
   Size:        262     371
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      48     185     236     479     633 
   Size:         48     137      51     243     154

  Fragments arranged by size: 

                 243     154     137      51      48
 
BsrBI CCG'CTC

Cuts at:      0      96     135     633 
   Size:         96      39     498

  Fragments arranged by size: 

                 498      96      39
 
BsrFI r'CCGG_y

Cuts at:      0     197     217     633 
   Size:        197      20     416

  Fragments arranged by size: 

                 416     197      20
 
BsrGI T'GTAC_A

Cuts at:      0     608     626     633 
   Size:        608      18       7

  Fragments arranged by size: 

                 608      18       7
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     237     633 
   Size:        237     396
 
BstYI r'GATC_y

Cuts at:      0     233     585     633 
   Size:        233     352      48

  Fragments arranged by size: 

                 352     233      48
 
BtgI C'CryG_G

Cuts at:      0     144     633 
   Size:        144     489
 
BtsI GCAGTG_nn'

Cuts at:      0     317     633 
   Size:        317     316
 
EaeI y'GGCC_r

Cuts at:      0      97     483     633 
   Size:         97     386     150

  Fragments arranged by size: 

                 386     150      97
 
EagI C'GGCC_G

Cuts at:      0      97     633 
   Size:         97     536
 
EarI CTCTTCn'nnn_

Cuts at:      0     286     425     633 
   Size:        286     139     208

  Fragments arranged by size: 

                 286     208     139
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     145     633 
   Size:        145     488
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     633 
   Size:         61     572
 
EcoO109I rG'GnC_Cy

Cuts at:      0     615     633 
   Size:        615      18
 
EcoRI G'AATT_C

Cuts at:      0     295     633 
   Size:        295     338
 
FspI TGC'GCA

Cuts at:      0     164     633 
   Size:        164     469
 
HaeII r_GCGC'y

Cuts at:      0     143     536     633 
   Size:        143     393      97

  Fragments arranged by size: 

                 393     143      97
 
KasI G'GCGC_C

Cuts at:      0     532     633 
   Size:        532     101
 
MslI CAynn'nnrTG

Cuts at:      0     304     633 
   Size:        304     329
 
MspA1I CmG'CkG

Cuts at:      0     105     146     168     633 
   Size:        105      41      22     465

  Fragments arranged by size: 

                 465     105      41      22
 
NaeI GCC'GGC

Cuts at:      0     199     633 
   Size:        199     434
 
NarI GG'CG_CC

Cuts at:      0     533     633 
   Size:        533     100
 
NgoMIV G'CCGG_C

Cuts at:      0     197     633 
   Size:        197     436
 
NotI GC'GGCC_GC

Cuts at:      0      97     633 
   Size:         97     536
 
NsiI A_TGCA'T

Cuts at:      0     412     633 
   Size:        412     221
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      60     529     633 
   Size:         60     469     104

  Fragments arranged by size: 

                 469     104      60
 
PpuMI rG'GwC_Cy

Cuts at:      0     615     633 
   Size:        615      18
 
PshAI GACnn'nnGTC

Cuts at:      0     344     633 
   Size:        344     289
 
PstI C_TGCA'G

Cuts at:      0     233     633 
   Size:        233     400
 
PvuII CAG'CTG

Cuts at:      0     168     633 
   Size:        168     465
 
SacII CC_GC'GG

Cuts at:      0     147     633 
   Size:        147     486
 
SfcI C'TryA_G

Cuts at:      0     229     633 
   Size:        229     404
 
SfoI GGC'GCC

Cuts at:      0     534     633 
   Size:        534      99
 
TatI w'GTAC_w

Cuts at:      0     608     626     633 
   Size:        608      18       7

  Fragments arranged by size: 

                 608      18       7
 
XmnI GAAnn'nnTTC

Cuts at:      0     596     633 
   Size:        596      37
 

 Enzymes that do cut:

     AarI    AlwNI     ApoI    BamHI     BanI     BbeI    BfrBI     BglI
 Bme1580I     BpmI   Bpu10I     BsaI    BsaHI    BseYI     BsgI    BsiEI
     BsmI Bsp1286I    BspMI    BsrBI    BsrFI    BsrGI   BstAPI    BstYI
     BtgI     BtsI     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I
    EcoRI     FspI    HaeII     KasI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NotI     NsiI    PflMI    PpuMI    PshAI     PstI    PvuII
    SacII     SfcI     SfoI     TatI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BanII     BbsI    BbvCI
     BcgI    BciVI     BclI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI  BsiHKAI
    BsiWI    BsmBI    BspEI    BspHI    BsrDI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI  EcoICRI    EcoNI    EcoRV     FalI     FseI    FspAI
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     NcoI     NdeI     NheI     NruI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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