Restriction map for pF1KB7991
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaLI       ApoI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AviII       AvrII       BbrPI       BbsI        
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaBI       BsaI        BsiHKAI     BsiWI       BsmI        
Bsp1407I    BspDI       BspHI       BspLU11I    BspMI       
BsrGI       BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        Eam1105I    EarI        
Eco47III    Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HindIII     HpaI        HphI        KpnI        Ksp632I     
MaeI        MamI        MboII       MfeI        MluI        
MseI        MslI        MunI        NdeI        NheI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SalI        SapI        SbfI        ScaI        SexAI       
SfuI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        TfiI        
Tru9I       Tsp509I     Tth111I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccI          109  GTCTAC               GT^[AC][GT]AC
AccIII        187  TCCGGA               T^CCGGA
AfaI          444  GTAC                 GT^AC
AscI          449  GGCGCGCC             GG^CGCGCC
BamHI         941  GGATCC               G^GATCC
BbvCI         769  CCTCAGC              CCTCAGC(-5/-2)
BsaAI         112  TACGTG               [CT]AC^GT[GA]
BseAI         187  TCCGGA               T^CCGGA
BsgI          565  CTGCAC               GTGCAG(16/14)
BsmBI        1232  CGTCTC               CGTCTC(1/5)
BspEI         187  TCCGGA               T^CCGGA
BssHII        450  GCGCGC               G^CGCGC
BstYI         941  GGATCC               [GA]^GATC[CT]
DpnII         942  GATC                 ^GATC
DraIII        837  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
HincII        652  GTCAAC               GT[CT]^[GA]AC
HindII        652  GTCAAC               GT[CT]^[GA]AC
HinfI        1273  GACTC                G^A[ACGT]TC
MboI          942  GATC                 ^GATC
MflI          941  GGATCC               [GA]^GATC[CT]
MroI          187  TCCGGA               T^CCGGA
NdeII         942  GATC                 ^GATC
NotI          352  GCGGCCGC             GC^GGCCGC
PflMI         836  CCACGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         1273  GACTC                GAGTC(4/5)
RsaI          444  GTAC                 GT^AC
Sau3AI        942  GATC                 ^GATC
SfcI          393  CTACAG               C^T[GA][CT]AG
SgrAI         305  CGCCGGTG             C[GA]^CCGG[CT]G
StuI          916  AGGCCT               AGG^CCT
Van91I        836  CCACGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          338  CCACCGGGTCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         941  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AlwI          942  GATCC                GGATC(4/5)
              941  GGATC               
ApaI          235  GGGCCC               GGGCC^C
             1177  GGGCCC              
BalI           14  TGGCCA               TGG^CCA
             1170  TGGCCA              
BsaWI         187  TCCGGA               [AT]^CCGG[AT]
              211  TCCGGT              
BseRI         135  CTCCTC               GAGGAG(10/8)
             1075  GAGGAG              
BsmAI        1233  GTCTC                GTCTC(1/5)
             1248  GTCTC               
BsrI          106  CCAGT                ACTGG(1/-1)
             1245  CCAGT               
BstF5I       1185  CATCC                GGATG(2/0)
              262  GGATG               
DrdI          336  GACCACCGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              553  GACAGCCCGGTC        
EcoT14I        17  CCATGG               C^C[AT][AT]GG
             1173  CCATGG              
FokI         1185  CATCC                GGATG(9/13)
              262  GGATG               
KspI          124  CCGCGG               CCGC^GG
              371  CCGCGG              
MaeIII        701  GTCAC                ^GT[ACGT]AC
             1304  GTCAC               
MluNI          14  TGGCCA               TGG^CCA
             1170  TGGCCA              
MscI           14  TGGCCA               TGG^CCA
             1170  TGGCCA              
NcoI           17  CCATGG               C^CATGG
             1173  CCATGG              
SacII         124  CCGCGG               CCGC^GG
              371  CCGCGG              
SfaNI        1080  GATGC                GCATC(5/9)
              746  GCATC               
SfiI          424  GGCCTGGCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
              585  GGCCAACCCGGCC       
StyI           17  CCATGG               C^C[AT][AT]GG
             1173  CCATGG              
TaqI          551  TCGA                 T^CGA
              614  TCGA                
Tsp45I        701  GTCAC                ^GT[GC]AC
             1304  GTCAC               
TthHB8I       551  TCGA                 T^CGA
              614  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AvaI        
AvaII       BanI        BanII       BbeI        BbvI        
BcnI        BglI        BmyI        BpmI        BsaHI       
BsaJI       BsiEI       BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BsrBI       BsrDI       BsrFI       
BssKI       BstAPI      BstNI       BstUI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DraII       
DsaI        EaeI        EagI        EclXI       Eco52I      
EcoO109I    EcoRII      FauI        Fnu4HI      HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HpaII       ItaI        KasI        MaeII       
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NgoMIV      
NlaIII      NlaIV       PpuMI       Sau96I      ScrFI       
SfoI        SmaI        TseI        TspRI       XmaCI       
XmaI        
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