Restriction map for pF1KB7991
 (Linear) (Six-base) MAPSORT of: KIBB7991.seq  Check: 5236  from: 1  to: 1326



KIBB7991.orf

 With 182 enzymes: SgfI * 

                        November 19, 2010 15:54  ..

AccI GT'mk_AC

Cuts at:      0     110    1326 
   Size:        110    1216
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     986    1228    1326 
   Size:        986     242      98

  Fragments arranged by size: 

                 986     242      98
 
ApaI G_GGCC'C

Cuts at:      0     239    1181    1326 
   Size:        239     942     145

  Fragments arranged by size: 

                 942     239     145
 
AscI GG'CGCG_CC

Cuts at:      0     450    1326 
   Size:        450     876
 
AvaI C'yCGr_G

Cuts at:      0     198     238     258     330     377    1326 
   Size:        198      40      20      72      47     949

  Fragments arranged by size: 

                 949     198      72      47      40      20
 
BamHI G'GATC_C

Cuts at:      0     941    1326 
   Size:        941     385
 
BanI G'GyrC_C

Cuts at:      0      46      70      91     129     193     845    1326 
   Size:         46      24      21      38      64     652     481

  Fragments arranged by size: 

                 652     481      64      46      38      24      21
 
BanII G_rGCy'C

Cuts at:      0     181     239     469    1181    1326 
   Size:        181      58     230     712     145

  Fragments arranged by size: 

                 712     230     181     145      58
 
BbeI G_GCGC'C

Cuts at:      0      74      95     197     849    1326 
   Size:         74      21     102     652     477

  Fragments arranged by size: 

                 652     477     102      74      21
 
BbvCI CC'TCA_GC

Cuts at:      0     770    1326 
   Size:        770     556
 
BglI GCCn_nnn'nGGC

Cuts at:      0      55     431     578     592     800    1326 
   Size:         55     376     147      14     208     526

  Fragments arranged by size: 

                 526     376     208     147      55      14
 
Bme1580I G_kGCm'C

Cuts at:      0     119     134     239    1127    1181    1326 
   Size:        119      15     105     888      54     145

  Fragments arranged by size: 

                 888     145     119     105      54      15
 
BmgBI CAC'GTC

Cuts at:      0     504    1326 
   Size:        504     822
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     820    1037    1326 
   Size:        820     217     289

  Fragments arranged by size: 

                 820     289     217
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     459     702    1032    1326 
   Size:        145     314     243     330     294

  Fragments arranged by size: 

                 330     314     294     243     145
 
Bpu10I CC'TnA_GC

Cuts at:      0     770    1326 
   Size:        770     556
 
BsaAI yAC'GTr

Cuts at:      0     114    1326 
   Size:        114    1212
 
BsaHI Gr'CG_yC

Cuts at:      0      71      92     194     846    1326 
   Size:         71      21     102     652     480

  Fragments arranged by size: 

                 652     480     102      71      21
 
BsaWI w'CCGG_w

Cuts at:      0     187     211    1326 
   Size:        187      24    1115

  Fragments arranged by size: 

                1115     187      24
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1117    1147    1326 
   Size:       1117      30     179

  Fragments arranged by size: 

                1117     179      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     126    1090    1326 
   Size:        126     964     236

  Fragments arranged by size: 

                 964     236     126
 
BseYI C'CCAG_C

Cuts at:      0     144     245    1326 
   Size:        144     101    1081

  Fragments arranged by size: 

                1081     144     101
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     550    1326 
   Size:        550     776
 
BsiEI CG_ry'CG

Cuts at:      0     356     582     596     757    1326 
   Size:        356     226      14     161     569

  Fragments arranged by size: 

                 569     356     226     161      14
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1238    1326 
   Size:       1238      88
 
Bsp1286I G_dGCh'C

Cuts at:      0     119     134     181     239     469    1127    1181    1326 
   Size:        119      15      47      58     230     658      54     145

  Fragments arranged by size: 

                 658     230     145     119      58      54      47      15
            
 
BspEI T'CCGG_A

Cuts at:      0     187    1326 
   Size:        187    1139
 
BsrBI CCG'CTC

Cuts at:      0     412     678     759    1326 
   Size:        412     266      81     567

  Fragments arranged by size: 

                 567     412     266      81
 
BsrDI GCAATG_nn'

Cuts at:      0     858     882     932    1326 
   Size:        858      24      50     394

  Fragments arranged by size: 

                 858     394      50      24
 
BsrFI r'CCGG_y

Cuts at:      0     306     454     541     752     776    1326 
   Size:        306     148      87     211      24     550

  Fragments arranged by size: 

                 550     306     211     148      87      24
 
BssHII G'CGCG_C

Cuts at:      0     450    1326 
   Size:        450     876
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     573     719     881    1326 
   Size:        573     146     162     445

  Fragments arranged by size: 

                 573     445     162     146
 
BstYI r'GATC_y

Cuts at:      0     941    1326 
   Size:        941     385
 
BtgI C'CryG_G

Cuts at:      0      17      30     124     371     903    1173    1326 
   Size:         17      13      94     247     532     270     153

  Fragments arranged by size: 

                 532     270     247     153      94      17      13
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1177    1326 
   Size:       1177     149
 
BtsI GCAGTG_nn'

Cuts at:      0     405    1014    1319    1326 
   Size:        405     609     305       7

  Fragments arranged by size: 

                 609     405     305       7
 
DraIII CAC_nnn'GTG

Cuts at:      0     842    1326 
   Size:        842     484
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     342     559    1326 
   Size:        342     217     767

  Fragments arranged by size: 

                 767     342     217
 
EaeI y'GGCC_r

Cuts at:      0      14      20     353     497     579     593     754    1170 
   Size:         14       6     333     144      82      14     161     416

Cuts at:   1170    1295    1326 
   Size:        125      31

  Fragments arranged by size: 

                 416     333     161     144     125      82      31      14
                  14       6
 
EagI C'GGCC_G

Cuts at:      0     353     579     593     754    1326 
   Size:        353     226      14     161     572

  Fragments arranged by size: 

                 572     353     226     161      14
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     182     422     528     774    1326 
   Size:        182     240     106     246     552

  Fragments arranged by size: 

                 552     246     240     182     106
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     459     702     986    1032    1228    1326 
   Size:        145     314     243     284      46     196      98

  Fragments arranged by size: 

                 314     284     243     196     145      98      46
 
EcoO109I rG'GnC_Cy

Cuts at:      0     235     242     344     909    1178    1326 
   Size:        235       7     102     565     269     148

  Fragments arranged by size: 

                 565     269     235     148     102       7
 
HaeII r_GCGC'y

Cuts at:      0      74      95     197     777     849    1326 
   Size:         74      21     102     580      72     477

  Fragments arranged by size: 

                 580     477     102      74      72      21
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     616     635     648     667    1326 
   Size:        616      19      13      19     659

  Fragments arranged by size: 

                 659     616      19      19      13
 
HincII GTy'rAC

Cuts at:      0     654    1326 
   Size:        654     672
 
KasI G'GCGC_C

Cuts at:      0      70      91     193     845    1326 
   Size:         70      21     102     652     481

  Fragments arranged by size: 

                 652     481     102      70      21
 
MscI TGG'CCA

Cuts at:      0      16    1172    1326 
   Size:         16    1156     154

  Fragments arranged by size: 

                1156     154      16
 
MspA1I CmG'CkG

Cuts at:      0     126     367     373    1031    1326 
   Size:        126     241       6     658     295

  Fragments arranged by size: 

                 658     295     241     126       6
 
NaeI GCC'GGC

Cuts at:      0     456     543     778    1326 
   Size:        456      87     235     548

  Fragments arranged by size: 

                 548     456     235      87
 
NarI GG'CG_CC

Cuts at:      0      71      92     194     846    1326 
   Size:         71      21     102     652     480

  Fragments arranged by size: 

                 652     480     102      71      21
 
NcoI C'CATG_G

Cuts at:      0      17    1173    1326 
   Size:         17    1156     153

  Fragments arranged by size: 

                1156     153      17
 
NgoMIV G'CCGG_C

Cuts at:      0     454     541     776    1326 
   Size:        454      87     235     550

  Fragments arranged by size: 

                 550     454     235      87
 
NotI GC'GGCC_GC

Cuts at:      0     353    1326 
   Size:        353     973
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     842    1326 
   Size:        842     484
 
PpuMI rG'GwC_Cy

Cuts at:      0     242     344     909    1326 
   Size:        242     102     565     417

  Fragments arranged by size: 

                 565     417     242     102
 
PspOMI G'GGCC_C

Cuts at:      0     235    1177    1326 
   Size:        235     942     149

  Fragments arranged by size: 

                 942     235     149
 
SacII CC_GC'GG

Cuts at:      0     127     374    1326 
   Size:        127     247     952

  Fragments arranged by size: 

                 952     247     127
 
SanDI GG'GwC_CC

Cuts at:      0     242     344     909    1326 
   Size:        242     102     565     417

  Fragments arranged by size: 

                 565     417     242     102
 
SfcI C'TryA_G

Cuts at:      0     393    1326 
   Size:        393     933
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     431     592    1326 
   Size:        431     161     734

  Fragments arranged by size: 

                 734     431     161
 
SfoI GGC'GCC

Cuts at:      0      72      93     195     847    1326 
   Size:         72      21     102     652     479

  Fragments arranged by size: 

                 652     479     102      72      21
 
SgrAI Cr'CCGG_yG

Cuts at:      0     306    1326 
   Size:        306    1020
 
SmaI CCC'GGG

Cuts at:      0     240     260     332     379    1326 
   Size:        240      20      72      47     947

  Fragments arranged by size: 

                 947     240      72      47      20
 
StuI AGG'CCT

Cuts at:      0     918    1326 
   Size:        918     408
 
StyI C'CwwG_G

Cuts at:      0      17    1173    1326 
   Size:         17    1156     153

  Fragments arranged by size: 

                1156     153      17
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1117    1149    1326 
   Size:       1117      32     177

  Fragments arranged by size: 

                1117     177      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     345    1326 
   Size:        345     981
 
XmaI C'CCGG_G

Cuts at:      0     238     258     330     377    1326 
   Size:        238      20      72      47     949

  Fragments arranged by size: 

                 949     238      72      47      20
 

 Enzymes that do cut:

     AccI     AcuI     ApaI     AscI     AvaI    BamHI     BanI    BanII
     BbeI    BbvCI     BglI Bme1580I    BmgBI     BplI     BpmI   Bpu10I
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
    BsmBI Bsp1286I    BspEI    BsrBI    BsrDI    BsrFI   BssHII   BstAPI
    BstYI     BtgI    BtgZI     BtsI   DraIII     DrdI     EaeI     EagI
     EciI  Eco57MI EcoO109I    HaeII    Hin4I   HincII     KasI     MscI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI    PflMI    PpuMI
   PspOMI    SacII    SanDI     SfcI     SfiI     SfoI    SgrAI     SmaI
     StuI     StyI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI    AvrII     BaeI     BbsI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI     BmrI     BmtI    BpuEI     BsaI    BsaBI  BsiHKAI
    BsiWI     BsmI    BspHI    BspMI    BsrGI    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     ClaI    CspCI     DraI     EarI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MslI     NdeI     NheI
     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI    SexAI
     SgfI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
    TaqII     TatI  Tth111I     XbaI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com