Restriction map for pF1KE3342
 (Circular) (Six-base) MAPSORT of: pF1KE3342.seq  Check: 7375  from: 1  to: 6074



pF1KE3342  6074 bp

 With 182 enzymes: SgfI * 

                        March 2, 2011 13:37  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1966    1966 
   Size:       6074
 
AatII G_ACGT'C

Cuts at:   1570    1570 
   Size:       6074
 
AccI GT'mk_AC

Cuts at:   1874    3112    1874 
   Size:       1238    4836
 
Acc65I G'GTAC_C

Cuts at:   1404    2294    3090    1404 
   Size:        890     796    4388

  Fragments arranged by size: 

                4388     890     796
 
AclI AA'CG_TT

Cuts at:   5914    5914 
   Size:       6074
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    217    1582    1618    2074    3899    4331    5078    5346     217 
   Size:       1365      36     456    1825     432     747     268     945

  Fragments arranged by size: 

                1825    1365     945     747     456     432     268      36
            
 
AflIII A'CryG_T

Cuts at:    362    3426    4530    5507     362 
   Size:       3064    1104     977     929

  Fragments arranged by size: 

                3064    1104     977     929
 
AgeI A'CCGG_T

Cuts at:   4462    4462 
   Size:       6074
 
AhdI GACnn_n'nnGTC

Cuts at:    599     599 
   Size:       6074
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    682    1806     682 
   Size:       1124    4950
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1102    1134    1327    1359    1102 
   Size:         32     193      32    5817

  Fragments arranged by size: 

                5817     193      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:   2899    3379    4946    2899 
   Size:        480    1567    4027

  Fragments arranged by size: 

                4027    1567     480
 
ApaI G_GGCC'C

Cuts at:   1364    1658    1364 
   Size:        294    5780
 
ApaLI G'TGCA_C

Cuts at:   1058    4844    1058 
   Size:       3786    2288
 
ApoI r'AATT_y

Cuts at:   1921    3078    5427    5683    1921 
   Size:       1157    2349     256    2312

  Fragments arranged by size: 

                2349    2312    1157     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6074
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6074
 
AvaI C'yCGr_G

Cuts at:    306     391     410     743    1052    1256    1478    2283    2771 
   Size:         85      19     333     309     204     222     805     488

Cuts at:   2771    3036    3094     306 
   Size:        265      58    3286

  Fragments arranged by size: 

                3286     805     488     333     309     265     222     204
                  85      58      19
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   1856    1889    1856 
   Size:         33    6041
 
BamHI G'GATC_C

Cuts at:   3099    3099 
   Size:       6074
 
BanI G'GyrC_C

Cuts at:    352     652     928    1404    1482    1531    2203    2294    3090 
   Size:        300     276     476      78      49     672      91     796

Cuts at:   3090    3504    3752    3787     352 
   Size:        414     248      35    2639

  Fragments arranged by size: 

                2639     796     672     476     414     300     276     248
                  91      78      49      35
 
BanII G_rGCy'C

Cuts at:    116    1274    1364    1658    2437    3088    4118     116 
   Size:       1158      90     294     779     651    1030    2072

  Fragments arranged by size: 

                2072    1158    1030     779     651     294      90
 
BbeI G_GCGC'C

Cuts at:    356    1535    2207    3508    3756     356 
   Size:       1179     672    1301     248    2674

  Fragments arranged by size: 

                2674    1301    1179     672     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    438    1570    1904     438 
   Size:       1132     334    4608

  Fragments arranged by size: 

                4608    1132     334
 
BbvCI CC'TCA_GC

Cuts at:    893    2939     893 
   Size:       2046    4028
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1725    1759    1811    1845    1725 
   Size:         34      52      34    5954

  Fragments arranged by size: 

                5954      52      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   1353    3972    4733    6074    1353 
   Size:       2619     761    1341    1353

  Fragments arranged by size: 

                2619    1353    1341     761
 
BclI T'GATC_A

Cuts at:   2594    3594    2594 
   Size:       1000    5074
 
BglI GCCn_nnn'nGGC

Cuts at:    661    1112    2147    2476     661 
   Size:        451    1035     329    4259

  Fragments arranged by size: 

                4259    1035     451     329
 
BglII A'GATC_T

Cuts at:   2930    3589    5626    2930 
   Size:        659    2037    3378

  Fragments arranged by size: 

                3378    2037     659
 
BlpI GC'TnA_GC

Cuts at:    613    3186     613 
   Size:       2573    3501
 
Bme1580I G_kGCm'C

Cuts at:    306     761     924     931    1041    1062    1143    1364    1485 
   Size:        455     163       7     110      21      81     221     121

Cuts at:   1485    1658    1738    3699    3792    4848     306 
   Size:        173      80    1961      93    1056    1532

  Fragments arranged by size: 

                1961    1532    1056     455     221     173     163     121
                 110      93      81      80      21       7
 
BmgBI CAC'GTC

Cuts at:    294    2592     294 
   Size:       2298    3776
 
BmrI ACTGGGnnnn_n'

Cuts at:   1481    2580    3286    3367    3702    5846    1481 
   Size:       1099     706      81     335    2144    1709

  Fragments arranged by size: 

                2144    1709    1099     706     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1186    2427    4441    1186 
   Size:       1241    2014    2819

  Fragments arranged by size: 

                2819    2014    1241
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1168    2164    2422    2538    1168 
   Size:        996     258     116    4704

  Fragments arranged by size: 

                4704     996     258     116
 
Bpu10I CC'TnA_GC

Cuts at:    893    1350    2939     893 
   Size:        457    1589    4028

  Fragments arranged by size: 

                4028    1589     457
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1476    1993    3250    4129    4621    4919    1476 
   Size:        517    1257     879     492     298    2631

  Fragments arranged by size: 

                2631    1257     879     517     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    154     427    1226    2318    5778     154 
   Size:        273     799    1092    3460     450

  Fragments arranged by size: 

                3460    1092     799     450     273
 
BsaAI yAC'GTr

Cuts at:    363    3427     363 
   Size:       3064    3010
 
BsaBI GATnn'nnATC

Cuts at:      6    2455    3588    5625       6 
   Size:       2449    1133    2037     455

  Fragments arranged by size: 

                2449    2037    1133     455
 
BsaHI Gr'CG_yC

Cuts at:    353    1532    1567    2204    3505    3753    4455    5951     353 
   Size:       1179      35     637    1301     248     702    1496     476

  Fragments arranged by size: 

                1496    1301    1179     702     637     476     248      35
            
 
BsaWI w'CCGG_w

Cuts at:   2489    2687    2741    3263    3784    4462    4736    4883    5294 
   Size:        198      54     522     521     678     274     147     411

Cuts at:   5294    2489 
   Size:       3269

  Fragments arranged by size: 

                3269     678     522     521     411     274     198     147
                  54
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1059    1089    1102    1132    2419    2449    2533    2551    2563 
   Size:         30      13      30    1287      30      84      18      12

Cuts at:   2563    2581    2783    2813    1059 
   Size:         18     202      30    4320

  Fragments arranged by size: 

                4320    1287     202      84      30      30      30      30
                  18      18      13      12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1107    2419    1107 
   Size:       1312    4762
 
BseYI C'CCAG_C

Cuts at:    131     415     701     805     811     972    1447    1543    3499 
   Size:        284     286     104       6     161     475      96    1956

Cuts at:   3499    4257    4834    5330     131 
   Size:        758     577     496     875

  Fragments arranged by size: 

                1956     875     758     577     496     475     286     284
                 161     104      96       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    316     661    1050    1108    1642    1994     316 
   Size:        345     389      58     534     352    4396

  Fragments arranged by size: 

                4396     534     389     352     345      58
 
BsiEI CG_ry'CG

Cuts at:    104    3616    3662    4870     104 
   Size:       3512      46    1208    1308

  Fragments arranged by size: 

                3512    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    116     876    1062    2126    2437    3088    3866    4056    4848 
   Size:        760     186    1064     311     651     778     190     792

Cuts at:   4848     116 
   Size:       1342

  Fragments arranged by size: 

                1342    1064     792     778     760     651     311     190
                 186
 
BsmI GAATG_Cn'

Cuts at:   2697    2697 
   Size:       6074
 
Bsp1286I G_dGCh'C

Cuts at:    116     306     761     876     924     931    1041    1062    1143 
   Size:        190     455     115      48       7     110      21      81

Cuts at:   1143    1274    1364    1485    1658    1738    2126    2437    3088 
   Size:        131      90     121     173      80     388     311     651

Cuts at:   3088    3699    3792    3866    4056    4118    4848     116 
   Size:        611      93      74     190      62     730    1342

  Fragments arranged by size: 

                1342     730     651     611     455     388     311     190
                 190     173     131     121     115     110      93      90
                  81      80      74      62      48      21       7
 
BspEI T'CCGG_A

Cuts at:   2489    2741    5294    2489 
   Size:        252    2553    3269

  Fragments arranged by size: 

                3269    2553     252
 
BspHI T'CATG_A

Cuts at:   2174    5250    2174 
   Size:       3076    2998
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1614    1966    2954    3124    3338    3640    4021    1614 
   Size:        352     988     170     214     302     381    3667

  Fragments arranged by size: 

                3667     988     381     352     302     214     170
 
BsrBI CCG'CTC

Cuts at:     50     245     612    2073    4366    4420    5904    6069      50 
   Size:        195     367    1461    2293      54    1484     165      55

  Fragments arranged by size: 

                2293    1484    1461     367     195     165      55      54
            
 
BsrDI GCAATG_nn'

Cuts at:   1815    2480    3986    1815 
   Size:        665    1506    3903

  Fragments arranged by size: 

                3903    1506     665
 
BsrFI r'CCGG_y

Cuts at:    766     852    1645    2618    3054    4072    4253    4462     766 
   Size:         86     793     973     436    1018     181     209    2378

  Fragments arranged by size: 

                2378    1018     973     793     436     209     181      86
            
 
BssHII G'CGCG_C

Cuts at:   1633    1633 
   Size:       6074
 
BssSI C'ACGA_G

Cuts at:   2257    2430    4345    4703    2257 
   Size:        173    1915     358    3628

  Fragments arranged by size: 

                3628    1915     358     173
 
BstAPI GCAn_nnn'nTGC

Cuts at:    270    3451     270 
   Size:       3181    2893
 
BstBI TT'CG_AA

Cuts at:   4435    4435 
   Size:       6074
 
BstEII G'GTnAC_C

Cuts at:   2154    2154 
   Size:       6074
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1617    2868    1617 
   Size:       1251    4823
 
BstYI r'GATC_y

Cuts at:      1    2930    3099    3566    3589    3924    4170    5182    5268 
   Size:       2929     169     467      23     335     246    1012      86

Cuts at:   5268    5280    5626       1 
   Size:         12     346     449

  Fragments arranged by size: 

                2929    1012     467     449     346     335     246     169
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    215    1795     215 
   Size:       1580    4494
 
BtgI C'CryG_G

Cuts at:    107     343     476     533     761    1087    1355     107 
   Size:        236     133      57     228     326     268    4826

  Fragments arranged by size: 

                4826     326     268     236     228     133      57
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    476    2262    4024    4205    4368     476 
   Size:       1786    1762     181     163    2182

  Fragments arranged by size: 

                2182    1786    1762     181     163
 
BtsI GCAGTG_nn'

Cuts at:    235    1325    1421    3317     235 
   Size:       1090      96    1896    2992

  Fragments arranged by size: 

                2992    1896    1090      96
 
ClaI AT'CG_AT

Cuts at:   1937    1937 
   Size:       6074
 
DraI TTT'AAA

Cuts at:   3073    3073 
   Size:       6074
 
DraIII CAC_nnn'GTG

Cuts at:    270     908    1489     270 
   Size:        638     581    4855

  Fragments arranged by size: 

                4855     638     581
 
DrdI GACnn_nn'nnGTC

Cuts at:   1510    3780    4638    1510 
   Size:       2270     858    2946

  Fragments arranged by size: 

                2946    2270     858
 
EaeI y'GGCC_r

Cuts at:    143     473     530     764     834    1158    1662    2993    3659 
   Size:        330      57     234      70     324     504    1331     666

Cuts at:   3659    3833    4224    4251    5596     143 
   Size:        174     391      27    1345     621

  Fragments arranged by size: 

                1345    1331     666     621     504     391     330     324
                 234     174      70      57      27
 
EagI C'GGCC_G

Cuts at:   3659    3659 
   Size:       6074
 
EarI CTCTTCn'nnn_

Cuts at:    152     688    1955    2418    4097    4307     152 
   Size:        536    1267     463    1679     210    1919

  Fragments arranged by size: 

                1919    1679    1267     536     463     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2128    2493    4282    4592    4738    5884    2128 
   Size:        365    1789     310     146    1146    2318

  Fragments arranged by size: 

                2318    1789    1146     365     310     146
 
EcoICRI GAG'CTC

Cuts at:    114    2435    3086     114 
   Size:       2321     651    3102

  Fragments arranged by size: 

                3102    2321     651
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    217    1168    1582    1618    2074    2164    2422    2538    3899 
   Size:        951     414      36     456      90     258     116    1361

Cuts at:   3899    4331    5078    5346     217 
   Size:        432     747     268     945

  Fragments arranged by size: 

                1361     951     945     747     456     432     414     268
                 258     116      90      36
 
EcoNI CCTnn'n_nnAGG

Cuts at:    253    1621     253 
   Size:       1368    4706
 
EcoO109I rG'GnC_Cy

Cuts at:    339     956    1167    1260    1360    1540    1655    2013    2786 
   Size:        617     211      93     100     180     115     358     773

Cuts at:   2786    2943    3213     339 
   Size:        157     270    3200

  Fragments arranged by size: 

                3200     773     617     358     270     211     180     157
                 115     100      93
 
EcoRI G'AATT_C

Cuts at:   3078    3078 
   Size:       6074
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    453     642    3006    4093     453 
   Size:        189    2364    1087    2434

  Fragments arranged by size: 

                2434    2364    1087     189
 
FspI TGC'GCA

Cuts at:    274    3855     274 
   Size:       3581    2493
 
FspAI rTGC'GCAy

Cuts at:    274     274 
   Size:       6074
 
HaeII r_GCGC'y

Cuts at:    356     423     558    1535    2207    2726    3508    3756    4778 
   Size:         67     135     977     672     519     782     248    1022

Cuts at:   4778    5757     356 
   Size:        979     673

  Fragments arranged by size: 

                1022     979     977     782     673     672     519     248
                 135      67
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    198     230     385     417     585     617     726     758     992 
   Size:         32     155      32     168      32     109      32     234

Cuts at:    992    1024    1835    1867    2533    2565    3916    3946    3948 
   Size:         32     811      32     666      32    1351      30       2

Cuts at:   3948    3978     198 
   Size:         30    2294

  Fragments arranged by size: 

                2294    1351     811     666     234     168     155     109
                  32      32      32      32      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   2352    3113    2352 
   Size:        761    5313
 
KasI G'GCGC_C

Cuts at:    352    1531    2203    3504    3752     352 
   Size:       1179     672    1301     248    2674

  Fragments arranged by size: 

                2674    1301    1179     672     248
 
KpnI G_GTAC'C

Cuts at:   1408    2298    3094    1408 
   Size:        890     796    4388

  Fragments arranged by size: 

                4388     890     796
 
MluI A'CGCG_T

Cuts at:   5507    5507 
   Size:       6074
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1445    4745    4929    1445 
   Size:       3300     184    2590

  Fragments arranged by size: 

                3300    2590     184
 
MscI TGG'CCA

Cuts at:    836    2995    3835     836 
   Size:       2159     840    3075

  Fragments arranged by size: 

                3075    2159     840
 
MslI CAynn'nnrTG

Cuts at:    517    1429    2866    4063    4190     517 
   Size:        912    1437    1197     127    2401

  Fragments arranged by size: 

                2401    1437    1197     912     127
 
MspA1I CmG'CkG

Cuts at:    535     705     869     892    1089    1187    1719    2079    2811 
   Size:        170     164      23     197      98     532     360     732

Cuts at:   2811    2899    3185    3499    3859    4872    5117     535 
   Size:         88     286     314     360    1013     245    1492

  Fragments arranged by size: 

                1492    1013     732     532     360     360     314     286
                 245     197     170     164      98      88      23
 
NaeI GCC'GGC

Cuts at:   1647    4255    1647 
   Size:       2608    3466
 
NarI GG'CG_CC

Cuts at:    353    1532    2204    3505    3753     353 
   Size:       1179     672    1301     248    2674

  Fragments arranged by size: 

                2674    1301    1179     672     248
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       6074
 
NgoMIV G'CCGG_C

Cuts at:   1645    4253    1645 
   Size:       2608    3466
 
NspI r_CATG'y

Cuts at:   2169    2268    2292    3127    3627    4534    2169 
   Size:         99      24     835     500     907    3709

  Fragments arranged by size: 

                3709     907     835     500      99      24
 
PasI CC'CwG_GG

Cuts at:    559     924    1274    2017    2439     559 
   Size:        365     350     743     422    4194

  Fragments arranged by size: 

                4194     743     422     365     350
 
PciI A'CATG_T

Cuts at:   4530    4530 
   Size:       6074
 
PflMI CCAn_nnn'nTGG

Cuts at:   2259    2462    2259 
   Size:        203    5871
 
PfoI T'CCnGG_A

Cuts at:    641     728    2399    2801     641 
   Size:         87    1671     402    3914

  Fragments arranged by size: 

                3914    1671     402      87
 
PmeI GTTT'AAAC

Cuts at:   3073    3073 
   Size:       6074
 
PmlI CAC'GTG

Cuts at:    363     363 
   Size:       6074
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1102    1134    5213    5245    1102 
   Size:         32    4079      32    1931

  Fragments arranged by size: 

                4079    1931      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    339     956    1167    1260    1540    2943     339 
   Size:        617     211      93     280    1403    3470

  Fragments arranged by size: 

                3470    1403     617     280     211      93
 
PspOMI G'GGCC_C

Cuts at:   1360    1654    1360 
   Size:        294    5780
 
PspXI vC'TCGA_Gb

Cuts at:    391     391 
   Size:       6074
 
PstI C_TGCA'G

Cuts at:    868    1416    3121    3806     868 
   Size:        548    1705     685    3136

  Fragments arranged by size: 

                3136    1705     685     548
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6074
 
PvuII CAG'CTG

Cuts at:    705     869     892    1719    3499    3859     705 
   Size:        164      23     827    1780     360    2920

  Fragments arranged by size: 

                2920    1780     827     360     164      23
 
RsrII CG'GwC_CG

Cuts at:   4269    4269 
   Size:       6074
 
SacI G_AGCT'C

Cuts at:    116    2437    3088     116 
   Size:       2321     651    3102

  Fragments arranged by size: 

                3102    2321     651
 
SacII CC_GC'GG

Cuts at:    536    1090     536 
   Size:        554    5520
 
SalI G'TCGA_C

Cuts at:   3111    3111 
   Size:       6074
 
SanDI GG'GwC_CC

Cuts at:    339    1540     339 
   Size:       1201    4873
 
SapI GCTCTTCn'nnn_

Cuts at:   4097    4307    4097 
   Size:        210    5864
 
SbfI CC_TGCA'GG

Cuts at:   3121    3121 
   Size:       6074
 
ScaI AGT'ACT

Cuts at:   5591    5591 
   Size:       6074
 
SexAI A'CCwGG_T

Cuts at:   1262    1616    1262 
   Size:        354    5720
 
SfcI C'TryA_G

Cuts at:     33     864    1412    3117    3802    4795    4986      33 
   Size:        831     548    1705     685     993     191    1121

  Fragments arranged by size: 

                1705    1121     993     831     685     548     191
 
SfoI GGC'GCC

Cuts at:    354    1533    2205    3506    3754     354 
   Size:       1179     672    1301     248    2674

  Fragments arranged by size: 

                2674    1301    1179     672     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6074
 
SmaI CCC'GGG

Cuts at:    308    1054    1258    1480    2773    3038    3096     308 
   Size:        746     204     222    1293     265      58    3286

  Fragments arranged by size: 

                3286    1293     746     265     222     204      58
 
SmlI C'TyrA_G

Cuts at:    391    1455    2008    3229    4144    4636    4898     391 
   Size:       1064     553    1221     915     492     262    1567

  Fragments arranged by size: 

                1567    1221    1064     915     553     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6074
 
SphI G_CATG'C

Cuts at:   2169    2268    2292    3127    3627    2169 
   Size:         99      24     835     500    4616

  Fragments arranged by size: 

                4616     835     500      99      24
 
SspI AAT'ATT

Cuts at:   1828    1828 
   Size:       6074
 
StuI AGG'CCT

Cuts at:   2101    2101 
   Size:       6074
 
StyI C'CwwG_G

Cuts at:    107    3208    3562     107 
   Size:       3101     354    2619

  Fragments arranged by size: 

                3101    2619     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    192    1266    1412    1461    1500    1738    3657    3790    4249 
   Size:       1074     146      49      39     238    1919     133     459

Cuts at:   4249    4458     192 
   Size:        209    1808

  Fragments arranged by size: 

                1919    1808    1074     459     238     209     146     133
                  49      39
 
TatI w'GTAC_w

Cuts at:   5589    5589 
   Size:       6074
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    214     246     823     855    1059    1091    2783    2815    2998 
   Size:         32     577      32     204      32    1692      32     183

Cuts at:   2998    3030    5591    5623     214 
   Size:         32    2561      32     665

  Fragments arranged by size: 

                2561    1692     665     577     204     183      32      32
                  32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1437    3871    1437 
   Size:       2434    3640
 
XbaI T'CTAG_A

Cuts at:   3105    3105 
   Size:       6074
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3569    3569 
   Size:       6074
 
XhoI C'TCGA_G

Cuts at:    391     391 
   Size:       6074
 
XmaI C'CCGG_G

Cuts at:    306    1052    1256    1478    2771    3036    3094     306 
   Size:        746     204     222    1293     265      58    3286

  Fragments arranged by size: 

                3286    1293     746     265     222     204      58
 
ZraI GAC'GTC

Cuts at:   1568    1568 
   Size:       6074
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    FspAI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI   PspOMI    PspXI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AleI     AscI    AvrII    BfrBI     BmtI    BsiWI
    BsmBI    BsrGI  BstZ17I    CspCI    EcoRV     FseI  HindIII     HpaI
     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI    PshAI
     PsiI     PsrI     SfiI    SgrAI    SnaBI     SrfI     SwaI     XmnI
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