Restriction map for pF1KE9573
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BbrPI       BbsI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsiEI       
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspHI       BspMI       BsrGI       BssHII      Bst1107I    
BstBI       BstXI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRV       
FbaI        FseI        FspI        HgaI        HincII      
HindII      HindIII     HpaI        KpnI        Ksp632I     
KspI        MfeI        MluI        MluNI       MscI        
MseI        MunI        NaeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tru9I       Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccII        1279  CGCG                 CG^CG
AccIII        297  TCCGGA               T^CCGGA
AcyI         1256  GGCGCC               G[GA]^CG[CT]C
ApaI         1217  GGGCCC               GGGCC^C
AspHI         628  GAGCTC               G[AT]GC[AT]^C
BbeI         1256  GGCGCC               GGCGC^C
BbvCI        1338  GCTGAGG              CCTCAGC(-5/-2)
BcgI         1368  CGAGGAGAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          487  CTAG                 C^TAG
BsaAI        1120  TACGTG               [CT]AC^GT[GA]
BsaHI        1256  GGCGCC               G[GA]^CG[CT]C
BsaI         1083  GAGACC               GGTCTC(1/5)
BsaWI         297  TCCGGA               [AT]^CCGG[AT]
BseAI         297  TCCGGA               T^CCGGA
BseRI        1369  GAGGAG               GAGGAG(10/8)
BsgI          547  CTGCAC               GTGCAG(16/14)
BsiHKAI       628  GAGCTC               G[AT]GC[AT]^C
BsmAI        1083  GAGAC                GTCTC(1/5)
BsmFI         641  GGGAC                GGGAC(10/14)
BspEI         297  TCCGGA               T^CCGGA
BsrBI        1269  CCGCTC               CCGCTC(-3/-3)
BsrDI         401  GCAATG               GCAATG(2/0)
BssSI         631  CTCGTG               CACGAG(-5/-1)
BstEII        540  GGTAACC              G^GT[ACGT]ACC
BstPI         540  GGTAACC              G^GT[ACGT]ACC
BstUI        1279  CGCG                 CG^CG
BstYI         722  GGATCT               [GA]^GATC[CT]
DraII         641  GGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI          903  CCATGG               C^C[GA][CT]GG
EcoO109I      641  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       540  GGTAACC              G^GT[ACGT]ACC
EcoRI         579  GAATTC               G^AATTC
EcoT22I       431  ATGCAT               ATGCA^T
Hin1I        1256  GGCGCC               G[GA]^CG[CT]C
KasI         1256  GGCGCC               G^GCGCC
MaeI          487  CTAG                 C^TAG
MflI          722  GGATCT               [GA]^GATC[CT]
MroI          297  TCCGGA               T^CCGGA
MvnI         1279  CGCG                 CG^CG
NarI         1256  GGCGCC               GG^CGCC
NcoI          903  CCATGG               C^CATGG
NdeI          434  CATATG               CA^TATG
NsiI          431  ATGCAT               ATGCA^T
PleI         1411  GAGTC                GAGTC(4/5)
PpuMI         641  GGGACCC              [GA]G^G[AT]CC[CT]
PstI         1283  CTGCAG               CTGCA^G
SacI          628  GAGCTC               GAGCT^C
SfcI         1283  CTGCAG               C^T[GA][CT]AG
SfoI         1256  GGCGCC               GGC^GCC
TaqI          375  TCGA                 T^CGA
Tsp45I        970  GTGAC                ^GT[GC]AC
TthHB8I       375  TCGA                 T^CGA
XhoII         722  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI          579  GAATTC               [GA]^AATT[CT]
             1229  AAATTC              
AflIII        557  ACATGT               A^C[GA][CT]GT
             1007  ACATGT              
AlwNI        1286  CAGCCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1333  CAGGAGCTG           
ApoI          579  GAATTC               [GA]^AATT[CT]
             1229  AAATTC              
BanI          813  GGTGCC               G^G[CT][GA]CC
             1256  GGCGCC              
BcnI          645  CCCGG                CC^[GC]GG
              960  CCGGG               
BpmI          260  CTGGAG               CTGGAG(16/14)
             1080  CTGGAG              
BsaBI         301  GATTTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              588  GATTATCATC          
BsmI          173  GAATGC               GAATGC(1/-1)
              429  GAATGC              
BspLU11I      557  ACATGT               A^CATGT
             1007  ACATGT              
BsrFI          20  GCCGGT               [GA]^CCGG[CT]
              255  ACCGGC              
BsrI          678  CCAGT                ACTGG(1/-1)
             1062  CCAGT               
BstAPI        768  GCAACGCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1285  GCAGCCGCTGC         
Cfr10I         20  GCCGGT               [GA]^CCGG[CT]
              255  ACCGGC              
FauI          187  CCCGC                CCCGC(4/6)
              807  CCCGC               
HaeII        1208  AGCGCC               [GA]GCGC^[CT]
             1256  GGCGCC              
MaeII         122  ACGT                 A^CGT
             1121  ACGT                
MamI          301  GATTTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              588  GATTATCATC          
MslI          483  CACCCTAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              741  CATTGTCGTG          
MspA1I        808  CCGCTG               C[AC]G^C[GT]G
             1289  CCGCTG              
NciI          645  CCCGG                CC^[GC]GG
              960  CCGGG               
NspI          557  ACATGT               [GA]CATG^[CT]
             1007  ACATGT              
SexAI         379  ACCTGGT              A^CC[AT]GGT
              602  ACCTGGT             
TspRI          32  CACTG                CA[GC]TG[ACGT][ACGT]^
             1234  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BanII       BbvI        BmyI        BsaJI       BsiYI       
BslI        Bsp1286I    BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeIII      MboI        MboII       
MnlI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TfiI        TseI        
Tsp509I     
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