Restriction map for pF1KE9573
 (Linear) (Six-base) MAPSORT of: KIEE9573.seq  Check: 678  from: 1  to: 1422



KIEE9573.orf

 With 182 enzymes: SgfI * 

                        September 11, 2018 18:37  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     445    1036    1422 
   Size:        445     591     386

  Fragments arranged by size: 

                 591     445     386
 
AflIII A'CryG_T

Cuts at:      0     557    1007    1422 
   Size:        557     450     415

  Fragments arranged by size: 

                 557     450     415
 
AleI CACnn'nnGTG

Cuts at:      0     487    1422 
   Size:        487     935
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     679    1422 
   Size:        679     743
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1291    1338    1422 
   Size:       1291      47      84

  Fragments arranged by size: 

                1291      84      47
 
ApaI G_GGCC'C

Cuts at:      0    1221    1422 
   Size:       1221     201
 
ApoI r'AATT_y

Cuts at:      0     579    1229    1422 
   Size:        579     650     193

  Fragments arranged by size: 

                 650     579     193
 
BanI G'GyrC_C

Cuts at:      0     813    1256    1422 
   Size:        813     443     166

  Fragments arranged by size: 

                 813     443     166
 
BanII G_rGCy'C

Cuts at:      0     107     632    1221    1422 
   Size:        107     525     589     201

  Fragments arranged by size: 

                 589     525     201     107
 
BbeI G_GCGC'C

Cuts at:      0    1260    1422 
   Size:       1260     162
 
BbvCI CC'TCA_GC

Cuts at:      0    1339    1422 
   Size:       1339      83
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1357    1391    1422 
   Size:       1357      34      31

  Fragments arranged by size: 

                1357      34      31
 
BfrBI ATG'CAT

Cuts at:      0     433    1422 
   Size:        433     989
 
Bme1580I G_kGCm'C

Cuts at:      0    1049    1221    1422 
   Size:       1049     172     201

  Fragments arranged by size: 

                1049     201     172
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281    1101    1422 
   Size:        281     820     321

  Fragments arranged by size: 

                 820     321     281
 
Bpu10I CC'TnA_GC

Cuts at:      0    1339    1422 
   Size:       1339      83
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1077    1422 
   Size:       1077     345
 
BsaAI yAC'GTr

Cuts at:      0    1122    1422 
   Size:       1122     300
 
BsaBI GATnn'nnATC

Cuts at:      0     305     592    1422 
   Size:        305     287     830

  Fragments arranged by size: 

                 830     305     287
 
BsaHI Gr'CG_yC

Cuts at:      0    1257    1422 
   Size:       1257     165
 
BsaWI w'CCGG_w

Cuts at:      0     297    1422 
   Size:        297    1125
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     617     647    1422 
   Size:        617      30     775

  Fragments arranged by size: 

                 775     617      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1384    1422 
   Size:       1384      38
 
BseYI C'CCAG_C

Cuts at:      0     242     797    1053    1422 
   Size:        242     555     256     369

  Fragments arranged by size: 

                 555     369     256     242
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     532    1422 
   Size:        532     890
 
BsiHKAI G_wGCw'C

Cuts at:      0     632    1422 
   Size:        632     790
 
BsmI GAATG_Cn'

Cuts at:      0     179     435    1422 
   Size:        179     256     987

  Fragments arranged by size: 

                 987     256     179
 
Bsp1286I G_dGCh'C

Cuts at:      0     107     632    1049    1221    1422 
   Size:        107     525     417     172     201

  Fragments arranged by size: 

                 525     417     201     172     107
 
BspEI T'CCGG_A

Cuts at:      0     297    1422 
   Size:        297    1125
 
BsrBI CCG'CTC

Cuts at:      0    1271    1422 
   Size:       1271     151
 
BsrDI GCAATG_nn'

Cuts at:      0     408    1422 
   Size:        408    1014
 
BsrFI r'CCGG_y

Cuts at:      0      20     255    1422 
   Size:         20     235    1167

  Fragments arranged by size: 

                1167     235      20
 
BssSI C'ACGA_G

Cuts at:      0     631    1422 
   Size:        631     791
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     774    1291    1422 
   Size:        774     517     131

  Fragments arranged by size: 

                 774     517     131
 
BstEII G'GTnAC_C

Cuts at:      0     540    1422 
   Size:        540     882
 
BstYI r'GATC_y

Cuts at:      0     722    1422 
   Size:        722     700
 
BtgI C'CryG_G

Cuts at:      0     903    1422 
   Size:        903     519
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1012    1422 
   Size:       1012     410
 
EcoICRI GAG'CTC

Cuts at:      0     630    1422 
   Size:        630     792
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     445    1036    1101    1422 
   Size:        281     164     591      65     321

  Fragments arranged by size: 

                 591     321     281     164      65
 
EcoO109I rG'GnC_Cy

Cuts at:      0     642    1422 
   Size:        642     780
 
EcoRI G'AATT_C

Cuts at:      0     579    1422 
   Size:        579     843
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     789    1422 
   Size:        789     633
 
HaeII r_GCGC'y

Cuts at:      0    1212    1260    1422 
   Size:       1212      48     162

  Fragments arranged by size: 

                1212     162      48
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     985    1017    1116    1148    1396    1422 
   Size:        985      32      99      32     248      26

  Fragments arranged by size: 

                 985     248      99      32      32      26
 
KasI G'GCGC_C

Cuts at:      0    1256    1422 
   Size:       1256     166
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     410    1115    1289    1422 
   Size:        118     292     705     174     133

  Fragments arranged by size: 

                 705     292     174     133     118
 
MslI CAynn'nnrTG

Cuts at:      0     487     745    1422 
   Size:        487     258     677

  Fragments arranged by size: 

                 677     487     258
 
MspA1I CmG'CkG

Cuts at:      0     810    1291    1422 
   Size:        810     481     131

  Fragments arranged by size: 

                 810     481     131
 
NarI GG'CG_CC

Cuts at:      0    1257    1422 
   Size:       1257     165
 
NcoI C'CATG_G

Cuts at:      0     903    1422 
   Size:        903     519
 
NdeI CA'TA_TG

Cuts at:      0     435    1422 
   Size:        435     987
 
NsiI A_TGCA'T

Cuts at:      0     435    1422 
   Size:        435     987
 
NspI r_CATG'y

Cuts at:      0     561    1011    1422 
   Size:        561     450     411

  Fragments arranged by size: 

                 561     450     411
 
PasI CC'CwG_GG

Cuts at:      0     498    1422 
   Size:        498     924
 
PciI A'CATG_T

Cuts at:      0     557    1007    1422 
   Size:        557     450     415

  Fragments arranged by size: 

                 557     450     415
 
PpuMI rG'GwC_Cy

Cuts at:      0     642    1422 
   Size:        642     780
 
PspOMI G'GGCC_C

Cuts at:      0    1217    1422 
   Size:       1217     205
 
PstI C_TGCA'G

Cuts at:      0    1287    1422 
   Size:       1287     135
 
SacI G_AGCT'C

Cuts at:      0     632    1422 
   Size:        632     790
 
SanDI GG'GwC_CC

Cuts at:      0     642    1422 
   Size:        642     780
 
SexAI A'CCwGG_T

Cuts at:      0     379     602    1422 
   Size:        379     223     820

  Fragments arranged by size: 

                 820     379     223
 
SfcI C'TryA_G

Cuts at:      0    1283    1422 
   Size:       1283     139
 
SfoI GGC'GCC

Cuts at:      0    1258    1422 
   Size:       1258     164
 
StyI C'CwwG_G

Cuts at:      0     195     525     903    1362    1422 
   Size:        195     330     378     459      60

  Fragments arranged by size: 

                 459     378     330     195      60
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      68     185    1422 
   Size:         68     117    1237

  Fragments arranged by size: 

                1237     117      68
 
TatI w'GTAC_w

Cuts at:      0     155     443     680     848    1422 
   Size:        155     288     237     168     574

  Fragments arranged by size: 

                 574     288     237     168     155
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     617     649     699     731    1422 
   Size:        617      32      50      32     691

  Fragments arranged by size: 

                 691     617      50      32      32
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     AlfI    AlwNI     ApaI     ApoI     BanI
    BanII     BbeI    BbvCI     BcgI    BfrBI Bme1580I     BpmI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI   BstEII    BstYI     BtgI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI    HaeII    Hin4I     KasI     MmeI     MslI
   MspA1I     NarI     NcoI     NdeI     NsiI     NspI     PasI     PciI
    PpuMI   PspOMI     PstI     SacI    SanDI    SexAI     SfcI     SfoI
     StyI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AloI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbsI    BciVI     BclI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI    BpuEI    BsiEI    BsiWI    BsmBI
    BspHI    BspMI    BsrGI   BssHII    BstBI    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI    EcoNI    EcoRV     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MscI     NaeI   NgoMIV
     NheI     NotI     NruI     PacI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII
    SacII     SalI     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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