Restriction map for pF1KB5966
 (Linear) (Six-base) MAPSORT of: KIBB5966.seq  Check: 2113  from: 1  to: 1665



KIBB5966.orf

 With 182 enzymes: SgfI * 

                        January 6, 2012 11:52  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1105    1665 
   Size:       1105     560
 
AccI GT'mk_AC

Cuts at:      0    1454    1665 
   Size:       1454     211
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     911     937    1665 
   Size:        911      26     728

  Fragments arranged by size: 

                 911     728      26
 
AleI CACnn'nnGTG

Cuts at:      0      10    1665 
   Size:         10    1655
 
AlwNI CAG_nnn'CTG

Cuts at:      0     238     960    1665 
   Size:        238     722     705

  Fragments arranged by size: 

                 722     705     238
 
ApaI G_GGCC'C

Cuts at:      0     232     361    1665 
   Size:        232     129    1304

  Fragments arranged by size: 

                1304     232     129
 
ApoI r'AATT_y

Cuts at:      0     679    1159    1665 
   Size:        679     480     506

  Fragments arranged by size: 

                 679     506     480
 
BanI G'GyrC_C

Cuts at:      0     222    1665 
   Size:        222    1443
 
BanII G_rGCy'C

Cuts at:      0     160     232     361     746    1075    1484    1665 
   Size:        160      72     129     385     329     409     181

  Fragments arranged by size: 

                 409     385     329     181     160     129      72
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     114    1665 
   Size:        114    1551
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1059    1093    1665 
   Size:       1059      34     572

  Fragments arranged by size: 

                1059     572      34
 
BclI T'GATC_A

Cuts at:      0    1178    1665 
   Size:       1178     487
 
BglI GCCn_nnn'nGGC

Cuts at:      0     371    1255    1665 
   Size:        371     884     410

  Fragments arranged by size: 

                 884     410     371
 
BlpI GC'TnA_GC

Cuts at:      0    1364    1665 
   Size:       1364     301
 
Bme1580I G_kGCm'C

Cuts at:      0     232     361    1665 
   Size:        232     129    1304

  Fragments arranged by size: 

                1304     232     129
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     364    1665 
   Size:        364    1301
 
BmtI G_CTAG'C

Cuts at:      0     757    1665 
   Size:        757     908
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     213     590     804    1541    1665 
   Size:         46     167     377     214     737     124

  Fragments arranged by size: 

                 737     377     214     167     124      46
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1498    1665 
   Size:       1498     167
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1564    1665 
   Size:       1564     101
 
BsaAI yAC'GTr

Cuts at:      0     111     602    1665 
   Size:        111     491    1063

  Fragments arranged by size: 

                1063     491     111
 
BsaHI Gr'CG_yC

Cuts at:      0     388    1206    1397    1665 
   Size:        388     818     191     268

  Fragments arranged by size: 

                 818     388     268     191
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1514    1544    1665 
   Size:       1514      30     121

  Fragments arranged by size: 

                1514     121      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1013    1665 
   Size:       1013     652
 
BseYI C'CCAG_C

Cuts at:      0     374     757    1164    1665 
   Size:        374     383     407     501

  Fragments arranged by size: 

                 501     407     383     374
 
BsiEI CG_ry'CG

Cuts at:      0    1197    1665 
   Size:       1197     468
 
BsiHKAI G_wGCw'C

Cuts at:      0     298    1075    1082    1484    1665 
   Size:        298     777       7     402     181

  Fragments arranged by size: 

                 777     402     298     181       7
 
BsmI GAATG_Cn'

Cuts at:      0     891    1665 
   Size:        891     774
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     379    1665 
   Size:        379    1286
 
Bsp1286I G_dGCh'C

Cuts at:      0     160     232     298     361     746    1075    1082    1484 
   Size:        160      72      66      63     385     329       7     402

Cuts at:   1484    1665 
   Size:        181

  Fragments arranged by size: 

                 402     385     329     181     160      72      66      63
                   7
 
BspHI T'CATG_A

Cuts at:      0    1175    1665 
   Size:       1175     490
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     246    1105    1665 
   Size:        246     859     560

  Fragments arranged by size: 

                 859     560     246
 
BsrFI r'CCGG_y

Cuts at:      0    1457    1665 
   Size:       1457     208
 
BsrGI T'GTAC_A

Cuts at:      0    1055    1665 
   Size:       1055     610
 
BssSI C'ACGA_G

Cuts at:      0    1068    1665 
   Size:       1068     597
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      12     111    1665 
   Size:         12      99    1554

  Fragments arranged by size: 

                1554      99      12
 
BtgI C'CryG_G

Cuts at:      0      11    1665 
   Size:         11    1654
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     900    1665 
   Size:        900     765
 
EaeI y'GGCC_r

Cuts at:      0     167     320     522     581    1665 
   Size:        167     153     202      59    1084

  Fragments arranged by size: 

                1084     202     167     153      59
 
EarI CTCTTCn'nnn_

Cuts at:      0     965    1665 
   Size:        965     700
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     705    1665 
   Size:        705     960
 
EcoICRI GAG'CTC

Cuts at:      0    1073    1482    1665 
   Size:       1073     409     183

  Fragments arranged by size: 

                1073     409     183
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     213     590     804     911     937    1541    1665 
   Size:         46     167     377     214     107      26     604     124

  Fragments arranged by size: 

                 604     377     214     167     124     107      46      26
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     235     358    1089    1665 
   Size:        235     123     731     576

  Fragments arranged by size: 

                 731     576     235     123
 
EcoRI G'AATT_C

Cuts at:      0    1159    1665 
   Size:       1159     506
 
EcoRV GAT'ATC

Cuts at:      0     697    1665 
   Size:        697     968
 
HaeII r_GCGC'y

Cuts at:      0     273     960    1665 
   Size:        273     687     705

  Fragments arranged by size: 

                 705     687     273
 
HindIII A'AGCT_T

Cuts at:      0    1559    1665 
   Size:       1559     106
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     425    1665 
   Size:        425    1240
 
MscI TGG'CCA

Cuts at:      0     322     583    1665 
   Size:        322     261    1082

  Fragments arranged by size: 

                1082     322     261
 
MslI CAynn'nnrTG

Cuts at:      0      10     109    1102    1665 
   Size:         10      99     993     563

  Fragments arranged by size: 

                 993     563      99      10
 
MspA1I CmG'CkG

Cuts at:      0     149     568    1168    1488    1665 
   Size:        149     419     600     320     177

  Fragments arranged by size: 

                 600     419     320     177     149
 
NheI G'CTAG_C

Cuts at:      0     753    1665 
   Size:        753     912
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     437     633    1665 
   Size:        437     196    1032

  Fragments arranged by size: 

                1032     437     196
 
PfoI T'CCnGG_A

Cuts at:      0      23     436    1665 
   Size:         23     413    1229

  Fragments arranged by size: 

                1229     413      23
 
PpuMI rG'GwC_Cy

Cuts at:      0     235    1665 
   Size:        235    1430
 
PspOMI G'GGCC_C

Cuts at:      0     228     357    1665 
   Size:        228     129    1308

  Fragments arranged by size: 

                1308     228     129
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1048    1080    1665 
   Size:       1048      32     585

  Fragments arranged by size: 

                1048     585      32
 
PstI C_TGCA'G

Cuts at:      0     204     567    1665 
   Size:        204     363    1098

  Fragments arranged by size: 

                1098     363     204
 
PvuII CAG'CTG

Cuts at:      0     568    1168    1665 
   Size:        568     600     497

  Fragments arranged by size: 

                 600     568     497
 
SacI G_AGCT'C

Cuts at:      0    1075    1484    1665 
   Size:       1075     409     181

  Fragments arranged by size: 

                1075     409     181
 
SexAI A'CCwGG_T

Cuts at:      0     419    1272    1665 
   Size:        419     853     393

  Fragments arranged by size: 

                 853     419     393
 
SfcI C'TryA_G

Cuts at:      0     200     545     563    1665 
   Size:        200     345      18    1102

  Fragments arranged by size: 

                1102     345     200      18
 
SmlI C'TyrA_G

Cuts at:      0    1477    1665 
   Size:       1477     188
 
StyI C'CwwG_G

Cuts at:      0     627    1216    1665 
   Size:        627     589     449

  Fragments arranged by size: 

                 627     589     449
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     366    1665 
   Size:        366    1299
 
TatI w'GTAC_w

Cuts at:      0    1055    1665 
   Size:       1055     610
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1393    1425    1514    1546    1665 
   Size:       1393      32      89      32     119

  Fragments arranged by size: 

                1393     119      89      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      19    1012    1665 
   Size:         19     993     653

  Fragments arranged by size: 

                 993     653      19
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     599    1665 
   Size:        599    1066
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AleI    AlwNI     ApaI     ApoI     BanI
    BanII     BbsI     BcgI     BclI     BglI     BlpI Bme1580I     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI
    BsrFI    BsrGI    BssSI    BstXI     BtgI    BtgZI     EaeI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV    HaeII  HindIII
     MmeI     MscI     MslI   MspA1I     NheI    PflMI     PfoI    PpuMI
   PspOMI     PsrI     PstI    PvuII     SacI    SexAI     SfcI     SmlI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbeI    BbvCI    BciVI    BfrBI    BglII    BmgBI
     BplI   Bpu10I    BsaBI    BsaWI     BsgI    BsiWI    BspEI    BsrBI
    BsrDI   BssHII   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI    EcoNI
     FalI     FseI     FspI    FspAI    Hin4I   HincII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     XbaI     XhoI     XmaI     XmnI     ZraI
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