Restriction map for pF1KE2049
##################################
# NO CUT ENZYMES (  103 enzymes) #
##################################

Acc65I      AccI        AccII       AclI        AflII       
AgeI        Aor51HI     AscI        AseI        AsnI        
Asp700I     Asp718I     AspI        AviII       AvrII       
BalI        BbsI        BcgI        BciVI       BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaBI       BsiWI       BspDI       BsrBI       BsrDI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     CelII       
ClaI        CpoI        DraIII      DrdI        Eco47III    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FseI        FspI        HindIII     HpaI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MscI        MunI        MvnI        NaeI        
NcoI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SbfI        SexAI       SfiI        SfuI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SspI        
StyI        SwaI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   64 enzymes) #
###################################

AatII         598  GACGTC               GACGT^C
AccIII        813  TCCGGA               T^CCGGA
AflIII         11  ACATGT               A^C[GA][CT]GT
AhdI         1235  GACGCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1345  GTGCAC               G^TGCAC
AlwNI        1511  CAGTGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1355  CCTCAGG              CC^T[ACGT]AGG
ApaI          148  GGGCCC               GGGCC^C
ApaLI        1345  GTGCAC               G^TGCAC
AspEI        1235  GACGCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          583  CCCGAG               C^[CT]CG[GA]G
BamHI        1437  GGATCC               G^GATCC
BbeI          579  GGCGCC               GGCGC^C
BbrPI         304  CACGTG               CAC^GTG
BbvCI         525  GCTGAGG              CCTCAGC(-5/-2)
BclI          125  TGATCA               T^GATCA
BcnI          219  CCGGG                CC^[GC]GG
BglI         1353  GCCCTCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         304  CACGTG               [CT]AC^GT[GA]
BsaWI         813  TCCGGA               [AT]^CCGG[AT]
BseAI         813  TCCGGA               T^CCGGA
BsiEI         823  CGGCCG               CG[GA][CT]^CG
BsmBI        1233  GAGACG               CGTCTC(1/5)
BsoBI         583  CCCGAG               C^[CT]CG[GA]G
BspEI         813  TCCGGA               T^CCGGA
BspLU11I       11  ACATGT               A^CATGT
BspMI        1450  GCAGGT               ACCTGC(4/8)
BsrFI         992  GCCGGT               [GA]^CCGG[CT]
BstYI        1437  GGATCC               [GA]^GATC[CT]
Bsu36I       1355  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        992  GCCGGT               [GA]^CCGG[CT]
DraI          909  TTTAAA               TTT^AAA
DsaI         1931  CCACGG               C^C[GA][CT]GG
EaeI          823  CGGCCG               [CT]^GGCC[GA]
EagI          823  CGGCCG               C^GGCCG
Eam1105I     1235  GACGCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         823  CGGCCG               C^GGCCG
Eco52I        823  CGGCCG               C^GGCCG
Eco81I       1355  CCTCAGG              CC^T[ACGT]AGG
EcoNI         177  CCTCTTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          125  TGATCA               T^GATCA
HincII        601  GTCAAC               GT[CT]^[GA]AC
HindII        601  GTCAAC               GT[CT]^[GA]AC
KasI          579  GGCGCC               G^GCGCC
MaeIII         44  GTGAC                ^GT[ACGT]AC
MflI         1437  GGATCC               [GA]^GATC[CT]
MroI          813  TCCGGA               T^CCGGA
NarI          579  GGCGCC               GG^CGCC
NciI          219  CCGGG                CC^[GC]GG
NspI           11  ACATGT               [GA]CATG^[CT]
PflMI        1457  CCAGAGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         304  CACGTG               CAC^GTG
PmlI          304  CACGTG               CAC^GTG
PstI         1120  CTGCAG               CTGCA^G
PvuII        1703  CAGCTG               CAG^CTG
SfoI          579  GGCGCC               GGC^GCC
SmlI         1376  CTCAAG               C^T[CT][GA]AG
SpeI         1738  ACTAGT               A^CTAGT
StuI          856  AGGCCT               AGG^CCT
TaqI         1976  TCGA                 T^CGA
Tsp45I         44  GTGAC                ^GT[GC]AC
TthHB8I      1976  TCGA                 T^CGA
Van91I       1457  CCAGAGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII        1437  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

BpmI           52  CTGGAG               CTGGAG(16/14)
             1087  CTGGAG              
BsaI         1519  GAGACC               GGTCTC(1/5)
             1242  GGTCTC              
BsmI          950  GAATGC               GAATGC(1/-1)
             1613  GCATTC              
Bsp1407I      140  TGTACA               T^GTACA
              632  TGTACA              
BsrGI         140  TGTACA               T^GTACA
              632  TGTACA              
BstXI        1038  CCAGGGACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1152  CCATCTTTATGG        
CfoI          580  GCGC                 GCG^C
             1954  GCGC                
HaeII         579  GGCGCC               [GA]GCGC^[CT]
             1953  GGCGCT              
HgaI         1235  GACGC                GACGC(5/10)
              239  GCGTC               
HhaI          580  GCGC                 GCG^C
             1954  GCGC                
HinP1I        580  GCGC                 G^CGC
             1954  GCGC                
MspA1I         72  CAGCGG               C[AC]G^C[GT]G
             1703  CAGCTG              
NdeI          680  CATATG               CA^TATG
             1486  CATATG              
PleI          119  GACTC                GAGTC(4/5)
             1066  GAGTC               
SapI          511  GAAGAGC              GCTCTTC(1/4)
             1696  GAAGAGC             
SfaNI         300  GATGC                GCATC(5/9)
             1890  GATGC               
SfcI          644  CTGTAG               C^T[GA][CT]AG
             1120  CTGCAG              
SspBI         140  TGTACA               T^GTACA
              632  TGTACA              
TfiI          810  GAATC                G^A[AT]TC
             1579  GAATC               
XcmI           41  CCAGTGACTTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1196  CCAGACAGAATGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        ApoI        AspHI       AvaII       BanI        
BanII       BbvI        BfaI        BmyI        BsaHI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspHI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EarI        
EcoO109I    EcoRII      Fnu4HI      FokI        HaeIII      
HapII       Hin1I       HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MboI        
MboII       MnlI        MseI        MslI        MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PpuMI       RcaI        RsaI        Sau3AI      Sau96I      
ScaI        ScrFI       Tru9I       TseI        Tsp509I     
TspRI       
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