Restriction map for
pF1KE2049
(Circular) (Six-base) MAPSORT of: pF1KE2049.seq Check: 5118 from: 1 to: 5126
pF1KE2049 5126 bp
With 182 enzymes: SgfI *
June 3, 2018 14:24 ..
AatII G_ACGT'C
Cuts at: 710 710
Size: 5126
AccI GT'mk_AC
Cuts at: 2164 2164
Size: 5126
Acc65I G'GTAC_C
Cuts at: 2142 2142
Size: 5126
AclI AA'CG_TT
Cuts at: 2120 4966 2120
Size: 2846 2280
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 2951 3383 4130 4398 544
Size: 2407 432 747 268 1272
Fragments arranged by size:
2407 1272 747 432 268
AflIII A'CryG_T
Cuts at: 119 2478 3582 4559 119
Size: 2359 1104 977 686
Fragments arranged by size:
2359 1104 977 686
AgeI A'CCGG_T
Cuts at: 3514 3514
Size: 5126
AhdI GACnn_n'nnGTC
Cuts at: 1348 1348
Size: 5126
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 424 424
Size: 5126
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1545 1577 1545
Size: 32 5094
AlwNI CAG_nnn'CTG
Cuts at: 1624 2431 3998 1624
Size: 807 1567 2752
Fragments arranged by size:
2752 1567 807
ApaI G_GGCC'C
Cuts at: 260 260
Size: 5126
ApaLI G'TGCA_C
Cuts at: 1453 3896 1453
Size: 2443 2683
ApoI r'AATT_y
Cuts at: 958 1107 1389 2130 4479 4735 958
Size: 149 282 741 2349 256 1349
Fragments arranged by size:
2349 1349 741 282 256 149
AseI AT'TA_AT
Cuts at: 20 20
Size: 5126
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5126
AvaI C'yCGr_G
Cuts at: 691 2146 691
Size: 1455 3671
BamHI G'GATC_C
Cuts at: 1545 2151 1545
Size: 606 4520
BanI G'GyrC_C
Cuts at: 649 670 687 2142 2556 2804 2839 649
Size: 21 17 1455 414 248 35 2936
Fragments arranged by size:
2936 1455 414 248 35 21 17
BanII G_rGCy'C
Cuts at: 260 1223 1472 2140 3170 260
Size: 963 249 668 1030 2216
Fragments arranged by size:
2216 1030 963 668 249
BbeI G_GCGC'C
Cuts at: 691 2560 2808 691
Size: 1869 248 3009
Fragments arranged by size:
3009 1869 248
BbvCI CC'TCA_GC
Cuts at: 634 634
Size: 5126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5092
BciVI GTATCCnnnnn_n'
Cuts at: 3024 3785 5126 3024
Size: 761 1341 3024
Fragments arranged by size:
3024 1341 761
BclI T'GATC_A
Cuts at: 233 2646 233
Size: 2413 2713
BglI GCCn_nnn'nGGC
Cuts at: 1467 1467
Size: 5126
BglII A'GATC_T
Cuts at: 2641 4678 2641
Size: 2037 3089
BlpI GC'TnA_GC
Cuts at: 2238 2238
Size: 5126
Bme1580I G_kGCm'C
Cuts at: 260 1457 1914 2751 2844 3900 260
Size: 1197 457 837 93 1056 1486
Fragments arranged by size:
1486 1197 1056 837 457 93
BmgBI CAC'GTC
Cuts at: 1008 1008
Size: 5126
BmrI ACTGGGnnnn_n'
Cuts at: 1612 1930 2338 2419 2754 4898 1612
Size: 318 408 81 335 2144 1840
Fragments arranged by size:
2144 1840 408 335 318 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3493 3493
Size: 5126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 1216 181
Size: 1035 4091
Bpu10I CC'TnA_GC
Cuts at: 634 634
Size: 5126
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1469 2302 3181 3673 3971 1469
Size: 833 879 492 298 2624
Fragments arranged by size:
2624 879 833 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1356 1621 4830 1356
Size: 265 3209 1652
Fragments arranged by size:
3209 1652 265
BsaAI yAC'GTr
Cuts at: 414 2479 414
Size: 2065 3061
BsaBI GATnn'nnATC
Cuts at: 6 2640 4677 6
Size: 2634 2037 455
Fragments arranged by size:
2634 2037 455
BsaHI Gr'CG_yC
Cuts at: 347 688 707 2557 2805 3507 5003 347
Size: 341 19 1850 248 702 1496 470
Fragments arranged by size:
1850 1496 702 470 341 248 19
BsaWI w'CCGG_w
Cuts at: 921 2315 2836 3514 3788 3935 4346 921
Size: 1394 521 678 274 147 411 1701
Fragments arranged by size:
1701 1394 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1165 1195 2027 2057 1165
Size: 30 832 30 4234
Fragments arranged by size:
4234 832 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 307 1186 1345 307
Size: 879 159 4088
Fragments arranged by size:
4088 879 159
BseYI C'CCAG_C
Cuts at: 908 1222 2551 3309 3886 4382 908
Size: 314 1329 758 577 496 1652
Fragments arranged by size:
1652 1329 758 577 496 314
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 1120 1451 188
Size: 932 331 3863
Fragments arranged by size:
3863 932 331
BsiEI CG_ry'CG
Cuts at: 104 934 2668 2714 3922 104
Size: 830 1734 46 1208 1308
Fragments arranged by size:
1734 1308 1208 830 46
BsiHKAI G_wGCw'C
Cuts at: 275 626 698 1202 1457 2140 2918 3108 3900
Size: 351 72 504 255 683 778 190 792
Cuts at: 3900 275
Size: 1501
Fragments arranged by size:
1501 792 778 683 504 351 255 190
72
BsmI GAATG_Cn'
Cuts at: 1064 1721 1064
Size: 657 4469
BsmBI CGTCTCn'nnnn_
Cuts at: 1335 1335
Size: 5126
Bsp1286I G_dGCh'C
Cuts at: 260 275 626 698 1202 1223 1457 1472 1914
Size: 15 351 72 504 21 234 15 442
Cuts at: 1914 2140 2751 2844 2918 3108 3170 3900 260
Size: 226 611 93 74 190 62 730 1486
Fragments arranged by size:
1486 730 611 504 442 351 234 226
190 93 74 72 62 21 15 15
BspEI T'CCGG_A
Cuts at: 921 4346 921
Size: 3425 1701
BspHI T'CATG_A
Cuts at: 230 236 896 4302 230
Size: 6 660 3406 1054
Fragments arranged by size:
3406 1054 660 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1549 2176 2390 2692 3073 1549
Size: 627 214 302 381 3602
Fragments arranged by size:
3602 627 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3418 3472 4956 5121 50
Size: 3368 54 1484 165 55
Fragments arranged by size:
3368 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3038 3038
Size: 5126
BsrFI r'CCGG_y
Cuts at: 1100 3124 3305 3514 1100
Size: 2024 181 209 2712
Fragments arranged by size:
2712 2024 209 181
BsrGI T'GTAC_A
Cuts at: 248 740 248
Size: 492 4634
BssSI C'ACGA_G
Cuts at: 3397 3755 3397
Size: 358 4768
BstAPI GCAn_nnn'nTGC
Cuts at: 2503 2503
Size: 5126
BstBI TT'CG_AA
Cuts at: 3487 3487
Size: 5126
BstXI CCAn_nnnn'nTGG
Cuts at: 1153 1267 1153
Size: 114 5012
BstYI r'GATC_y
Cuts at: 1 1545 2151 2618 2641 2976 3222 4234 4320
Size: 1544 606 467 23 335 246 1012 86
Cuts at: 4320 4332 4678 1
Size: 12 346 449
Fragments arranged by size:
1544 1012 606 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1464 1464
Size: 5126
BtgI C'CryG_G
Cuts at: 2039 2039
Size: 5126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3076 3257 3420 3076
Size: 181 163 4782
Fragments arranged by size:
4782 181 163
BtsI GCAGTG_nn'
Cuts at: 1132 1415 1457 2369 1132
Size: 283 42 912 3889
Fragments arranged by size:
3889 912 283 42
DraI TTT'AAA
Cuts at: 1019 2125 1019
Size: 1106 4020
DrdI GACnn_nn'nnGTC
Cuts at: 2832 3690 2832
Size: 858 4268
EaeI y'GGCC_r
Cuts at: 931 2711 2885 3276 3303 4648 931
Size: 1780 174 391 27 1345 1409
Fragments arranged by size:
1780 1409 1345 391 174 27
EagI C'GGCC_G
Cuts at: 931 2711 931
Size: 1780 3346
EarI CTCTTCn'nnn_
Cuts at: 614 995 1006 1048 1073 1205 1799 3149 3359
Size: 381 11 42 25 132 594 1350 210
Cuts at: 3359 614
Size: 2381
Fragments arranged by size:
2381 1350 594 381 210 132 42 25
11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3334 3644 3790 4936 3334
Size: 310 146 1146 3524
Fragments arranged by size:
3524 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2138 2138
Size: 5126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 544 1216 2951 3383 4130 4398 181
Size: 363 672 1735 432 747 268 909
Fragments arranged by size:
1735 909 747 672 432 363 268
EcoNI CCTnn'n_nnAGG
Cuts at: 289 289
Size: 5126
EcoO109I rG'GnC_Cy
Cuts at: 256 282 519 1162 1561 2265 256
Size: 26 237 643 399 704 3117
Fragments arranged by size:
3117 704 643 399 237 26
EcoRI G'AATT_C
Cuts at: 2130 2130
Size: 5126
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3145 3145
Size: 5126
FspI TGC'GCA
Cuts at: 2907 2907
Size: 5126
HaeII r_GCGC'y
Cuts at: 691 2065 2560 2808 3830 4809 691
Size: 1374 495 248 1022 979 1008
Fragments arranged by size:
1374 1022 1008 979 495 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 218 250 420 452 872 904 1334 1366 1620
Size: 32 170 32 420 32 430 32 254
Cuts at: 1620 1652 2968 2998 3000 3030 218
Size: 32 1316 30 2 30 2314
Fragments arranged by size:
2314 1316 430 420 254 170 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 711 2165 711
Size: 1454 3672
KasI G'GCGC_C
Cuts at: 687 2556 2804 687
Size: 1869 248 3009
Fragments arranged by size:
3009 1869 248
KpnI G_GTAC'C
Cuts at: 2146 2146
Size: 5126
MluI A'CGCG_T
Cuts at: 4559 4559
Size: 5126
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 713 3797 3981 713
Size: 3084 184 1858
Fragments arranged by size:
3084 1858 184
MscI TGG'CCA
Cuts at: 2887 2887
Size: 5126
MslI CAynn'nnrTG
Cuts at: 139 235 701 1265 1445 1961 3115 3242 139
Size: 96 466 564 180 516 1154 127 2023
Fragments arranged by size:
2023 1154 564 516 466 180 127 96
MspA1I CmG'CkG
Cuts at: 182 1813 2237 2551 2911 3924 4169 182
Size: 1631 424 314 360 1013 245 1139
Fragments arranged by size:
1631 1139 1013 424 360 314 245
NaeI GCC'GGC
Cuts at: 3307 3307
Size: 5126
NarI GG'CG_CC
Cuts at: 688 2557 2805 688
Size: 1869 248 3009
Fragments arranged by size:
3009 1869 248
NdeI CA'TA_TG
Cuts at: 789 1595 789
Size: 806 4320
NgoMIV G'CCGG_C
Cuts at: 3305 3305
Size: 5126
NspI r_CATG'y
Cuts at: 123 2179 2679 3586 123
Size: 2056 500 907 1663
Fragments arranged by size:
2056 1663 907 500
PasI CC'CwG_GG
Cuts at: 222 1146 222
Size: 924 4202
PciI A'CATG_T
Cuts at: 119 3582 119
Size: 3463 1663
PflMI CCAn_nnn'nTGG
Cuts at: 1571 1571
Size: 5126
PfoI T'CCnGG_A
Cuts at: 158 1548 2077 158
Size: 1390 529 3207
Fragments arranged by size:
3207 1390 529
PmeI GTTT'AAAC
Cuts at: 2125 2125
Size: 5126
PmlI CAC'GTG
Cuts at: 414 414
Size: 5126
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4265 4297 4265
Size: 32 5094
PpuMI rG'GwC_Cy
Cuts at: 519 1162 1561 519
Size: 643 399 4084
Fragments arranged by size:
4084 643 399
PspOMI G'GGCC_C
Cuts at: 256 256
Size: 5126
PstI C_TGCA'G
Cuts at: 1232 2173 2858 1232
Size: 941 685 3500
Fragments arranged by size:
3500 941 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5126
PvuII CAG'CTG
Cuts at: 1813 2551 2911 1813
Size: 738 360 4028
Fragments arranged by size:
4028 738 360
RsrII CG'GwC_CG
Cuts at: 3321 3321
Size: 5126
SacI G_AGCT'C
Cuts at: 2140 2140
Size: 5126
SalI G'TCGA_C
Cuts at: 2163 2163
Size: 5126
SapI GCTCTTCn'nnn_
Cuts at: 614 1799 3149 3359 614
Size: 1185 1350 210 2381
Fragments arranged by size:
2381 1350 1185 210
SbfI CC_TGCA'GG
Cuts at: 2173 2173
Size: 5126
ScaI AGT'ACT
Cuts at: 370 435 758 871 1490 1716 4643 370
Size: 65 323 113 619 226 2927 853
Fragments arranged by size:
2927 853 619 323 226 113 65
SfcI C'TryA_G
Cuts at: 33 752 1228 2169 2854 3847 4038 33
Size: 719 476 941 685 993 191 1121
Fragments arranged by size:
1121 993 941 719 685 476 191
SfoI GGC'GCC
Cuts at: 689 2558 2806 689
Size: 1869 248 3009
Fragments arranged by size:
3009 1869 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5126
SmaI CCC'GGG
Cuts at: 2148 2148
Size: 5126
SmlI C'TyrA_G
Cuts at: 1484 2281 3196 3688 3950 1484
Size: 797 915 492 262 2660
Fragments arranged by size:
2660 915 797 492 262
SpeI A'CTAG_T
Cuts at: 67 1846 67
Size: 1779 3347
SphI G_CATG'C
Cuts at: 2179 2679 2179
Size: 500 4626
StuI AGG'CCT
Cuts at: 966 966
Size: 5126
StyI C'CwwG_G
Cuts at: 2260 2614 2260
Size: 354 4772
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1217 1665 2001 2709 2842 3301 3510 1217
Size: 448 336 708 133 459 209 2833
Fragments arranged by size:
2833 708 459 448 336 209 133
TatI w'GTAC_w
Cuts at: 248 368 386 433 740 756 869 1488 1714
Size: 120 18 47 307 16 113 619 226
Cuts at: 1714 4641 248
Size: 2927 733
Fragments arranged by size:
2927 733 619 307 226 120 113 47
18 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 491 523 1893 1925 2027 2059 2091 4643 4675
Size: 32 1370 32 102 32 32 2552 32
Cuts at: 4675 491
Size: 942
Fragments arranged by size:
2552 1370 942 102 32 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 2923 2923
Size: 5126
XbaI T'CTAG_A
Cuts at: 2157 2157
Size: 5126
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 156 1311 2621 156
Size: 1155 1310 2661
Fragments arranged by size:
2661 1310 1155
XmaI C'CCGG_G
Cuts at: 2146 2146
Size: 5126
ZraI GAC'GTC
Cuts at: 708 708
Size: 5126
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AscI AvrII BaeI BbsI
BfrBI BmtI BsiWI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NheI NotI NruI NsiI PacI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI