Restriction map for pF1KE2049
 (Linear) (Six-base) MAPSORT of: KIEE2049.seq  Check: 9252  from: 1  to: 2013



KIEE2049.orf

 With 182 enzymes: SgfI * 

                        June 3, 2018 14:24  ..

AatII G_ACGT'C

Cuts at:      0     602    2013 
   Size:        602    1411
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436    2013 
   Size:        436    1577
 
AflIII A'CryG_T

Cuts at:      0      11    2013 
   Size:         11    2002
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1240    2013 
   Size:       1240     773
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     316    2013 
   Size:        316    1697
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1437    1469    2013 
   Size:       1437      32     544

  Fragments arranged by size: 

                1437     544      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1516    2013 
   Size:       1516     497
 
ApaI G_GGCC'C

Cuts at:      0     152    2013 
   Size:        152    1861
 
ApaLI G'TGCA_C

Cuts at:      0    1345    2013 
   Size:       1345     668
 
ApoI r'AATT_y

Cuts at:      0     850     999    1281    2013 
   Size:        850     149     282     732

  Fragments arranged by size: 

                 850     732     282     149
 
AvaI C'yCGr_G

Cuts at:      0     583    2013 
   Size:        583    1430
 
BamHI G'GATC_C

Cuts at:      0    1437    2013 
   Size:       1437     576
 
BanI G'GyrC_C

Cuts at:      0     541     562     579    2013 
   Size:        541      21      17    1434

  Fragments arranged by size: 

                1434     541      21      17
 
BanII G_rGCy'C

Cuts at:      0     152    1115    1364    2013 
   Size:        152     963     249     649

  Fragments arranged by size: 

                 963     649     249     152
 
BbeI G_GCGC'C

Cuts at:      0     583    2013 
   Size:        583    1430
 
BbvCI CC'TCA_GC

Cuts at:      0     526    2013 
   Size:        526    1487
 
BclI T'GATC_A

Cuts at:      0     125    2013 
   Size:        125    1888
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1359    2013 
   Size:       1359     654
 
Bme1580I G_kGCm'C

Cuts at:      0     152    1349    1806    2013 
   Size:        152    1197     457     207

  Fragments arranged by size: 

                1197     457     207     152
 
BmgBI CAC'GTC

Cuts at:      0     900    2013 
   Size:        900    1113
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1504    1822    2013 
   Size:       1504     318     191

  Fragments arranged by size: 

                1504     318     191
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73    1108    2013 
   Size:         73    1035     905

  Fragments arranged by size: 

                1035     905      73
 
Bpu10I CC'TnA_GC

Cuts at:      0     526    2013 
   Size:        526    1487
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1361    2013 
   Size:       1361     652
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1248    1513    2013 
   Size:       1248     265     500

  Fragments arranged by size: 

                1248     500     265
 
BsaAI yAC'GTr

Cuts at:      0     306    2013 
   Size:        306    1707
 
BsaHI Gr'CG_yC

Cuts at:      0     239     580     599    2013 
   Size:        239     341      19    1414

  Fragments arranged by size: 

                1414     341     239      19
 
BsaWI w'CCGG_w

Cuts at:      0     813    2013 
   Size:        813    1200
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1057    1087    1919    1949    2013 
   Size:       1057      30     832      30      64

  Fragments arranged by size: 

                1057     832      64      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     199    1078    1237    2013 
   Size:        199     879     159     776

  Fragments arranged by size: 

                 879     776     199     159
 
BseYI C'CCAG_C

Cuts at:      0     800    1114    2013 
   Size:        800     314     899

  Fragments arranged by size: 

                 899     800     314
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80    1012    1343    2013 
   Size:         80     932     331     670

  Fragments arranged by size: 

                 932     670     331      80
 
BsiEI CG_ry'CG

Cuts at:      0     826    2013 
   Size:        826    1187
 
BsiHKAI G_wGCw'C

Cuts at:      0     167     518     590    1094    1349    2013 
   Size:        167     351      72     504     255     664

  Fragments arranged by size: 

                 664     504     351     255     167      72
 
BsmI GAATG_Cn'

Cuts at:      0     956    1613    2013 
   Size:        956     657     400

  Fragments arranged by size: 

                 956     657     400
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1227    2013 
   Size:       1227     786
 
Bsp1286I G_dGCh'C

Cuts at:      0     152     167     518     590    1094    1115    1349    1364 
   Size:        152      15     351      72     504      21     234      15

Cuts at:   1364    1806    2013 
   Size:        442     207

  Fragments arranged by size: 

                 504     442     351     234     207     152      72      21
                  15      15
 
BspEI T'CCGG_A

Cuts at:      0     813    2013 
   Size:        813    1200
 
BspHI T'CATG_A

Cuts at:      0     122     128     788    2013 
   Size:        122       6     660    1225

  Fragments arranged by size: 

                1225     660     122       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1441    2013 
   Size:       1441     572
 
BsrFI r'CCGG_y

Cuts at:      0     992    2013 
   Size:        992    1021
 
BsrGI T'GTAC_A

Cuts at:      0     140     632    2013 
   Size:        140     492    1381

  Fragments arranged by size: 

                1381     492     140
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1045    1159    2013 
   Size:       1045     114     854

  Fragments arranged by size: 

                1045     854     114
 
BstYI r'GATC_y

Cuts at:      0    1437    2013 
   Size:       1437     576
 
Bsu36I CC'TnA_GG

Cuts at:      0    1356    2013 
   Size:       1356     657
 
BtgI C'CryG_G

Cuts at:      0    1931    2013 
   Size:       1931      82
 
BtsI GCAGTG_nn'

Cuts at:      0    1024    1307    1349    2013 
   Size:       1024     283      42     664

  Fragments arranged by size: 

                1024     664     283      42
 
DraI TTT'AAA

Cuts at:      0     911    2013 
   Size:        911    1102
 
EaeI y'GGCC_r

Cuts at:      0     823    2013 
   Size:        823    1190
 
EagI C'GGCC_G

Cuts at:      0     823    2013 
   Size:        823    1190
 
EarI CTCTTCn'nnn_

Cuts at:      0     506     887     898     940     965    1097    1691    2013 
   Size:        506     381      11      42      25     132     594     322

  Fragments arranged by size: 

                 594     506     381     322     132      42      25      11
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     436    1108    2013 
   Size:         73     363     672     905

  Fragments arranged by size: 

                 905     672     363      73
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     181    2013 
   Size:        181    1832
 
EcoO109I rG'GnC_Cy

Cuts at:      0     148     174     411    1054    1453    2013 
   Size:        148      26     237     643     399     560

  Fragments arranged by size: 

                 643     560     399     237     148      26
 
HaeII r_GCGC'y

Cuts at:      0     583    1957    2013 
   Size:        583    1374      56

  Fragments arranged by size: 

                1374     583      56
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     110     142     312     344     764     796    1226    1258 
   Size:        110      32     170      32     420      32     430      32

Cuts at:   1258    1512    1544    2013 
   Size:        254      32     469

  Fragments arranged by size: 

                 469     430     420     254     170     110      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     603    2013 
   Size:        603    1410
 
KasI G'GCGC_C

Cuts at:      0     579    2013 
   Size:        579    1434
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     605    2013 
   Size:        605    1408
 
MslI CAynn'nnrTG

Cuts at:      0      31     127     593    1157    1337    1853    2013 
   Size:         31      96     466     564     180     516     160

  Fragments arranged by size: 

                 564     516     466     180     160      96      31
 
MspA1I CmG'CkG

Cuts at:      0      74    1705    2013 
   Size:         74    1631     308

  Fragments arranged by size: 

                1631     308      74
 
NarI GG'CG_CC

Cuts at:      0     580    2013 
   Size:        580    1433
 
NdeI CA'TA_TG

Cuts at:      0     681    1487    2013 
   Size:        681     806     526

  Fragments arranged by size: 

                 806     681     526
 
NspI r_CATG'y

Cuts at:      0      15    2013 
   Size:         15    1998
 
PasI CC'CwG_GG

Cuts at:      0     114    1038    2013 
   Size:        114     924     975

  Fragments arranged by size: 

                 975     924     114
 
PciI A'CATG_T

Cuts at:      0      11    2013 
   Size:         11    2002
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1463    2013 
   Size:       1463     550
 
PfoI T'CCnGG_A

Cuts at:      0      50    1440    1969    2013 
   Size:         50    1390     529      44

  Fragments arranged by size: 

                1390     529      50      44
 
PmlI CAC'GTG

Cuts at:      0     306    2013 
   Size:        306    1707
 
PpuMI rG'GwC_Cy

Cuts at:      0     411    1054    1453    2013 
   Size:        411     643     399     560

  Fragments arranged by size: 

                 643     560     411     399
 
PspOMI G'GGCC_C

Cuts at:      0     148    2013 
   Size:        148    1865
 
PstI C_TGCA'G

Cuts at:      0    1124    2013 
   Size:       1124     889
 
PvuII CAG'CTG

Cuts at:      0    1705    2013 
   Size:       1705     308
 
SapI GCTCTTCn'nnn_

Cuts at:      0     506    1691    2013 
   Size:        506    1185     322

  Fragments arranged by size: 

                1185     506     322
 
ScaI AGT'ACT

Cuts at:      0     262     327     650     763    1382    1608    2013 
   Size:        262      65     323     113     619     226     405

  Fragments arranged by size: 

                 619     405     323     262     226     113      65
 
SfcI C'TryA_G

Cuts at:      0     644    1120    2013 
   Size:        644     476     893

  Fragments arranged by size: 

                 893     644     476
 
SfoI GGC'GCC

Cuts at:      0     581    2013 
   Size:        581    1432
 
SmlI C'TyrA_G

Cuts at:      0    1376    2013 
   Size:       1376     637
 
SpeI A'CTAG_T

Cuts at:      0    1738    2013 
   Size:       1738     275
 
StuI AGG'CCT

Cuts at:      0     858    2013 
   Size:        858    1155
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1109    1557    1893    2013 
   Size:       1109     448     336     120

  Fragments arranged by size: 

                1109     448     336     120
 
TatI w'GTAC_w

Cuts at:      0     140     260     278     325     632     648     761    1380 
   Size:        140     120      18      47     307      16     113     619

Cuts at:   1380    1606    2013 
   Size:        226     407

  Fragments arranged by size: 

                 619     407     307     226     140     120     113      47
                  18      16
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     383     415    1785    1817    1919    1951    1983    2013 
   Size:        383      32    1370      32     102      32      32      30

  Fragments arranged by size: 

                1370     383     102      32      32      32      32      30
            
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      48    1203    2013 
   Size:         48    1155     810

  Fragments arranged by size: 

                1155     810      48
 
ZraI GAC'GTC

Cuts at:      0     600    2013 
   Size:        600    1413
 

 Enzymes that do cut:

    AatII     AcuI   AflIII     AhdI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbeI    BbvCI
     BclI     BglI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrFI    BsrGI    BstXI    BstYI   Bsu36I     BtgI     BtsI     DraI
     EaeI     EagI     EarI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I
   HincII     KasI     MmeI     MslI   MspA1I     NarI     NdeI     NspI
     PasI     PciI    PflMI     PfoI     PmlI    PpuMI   PspOMI     PstI
    PvuII     SapI     ScaI     SfcI     SfoI     SmlI     SpeI     StuI
    TaqII     TatI     TstI     XcmI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AscI     AseI    AsiSI    AvrII     BaeI     BbsI     BcgI    BciVI
    BfrBI    BglII     BlpI     BmtI     BplI    BsaBI    BsiWI    BsrBI
    BsrDI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I    BtgZI
     ClaI    CspCI   DraIII     DrdI     EciI  EcoICRI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MfeI
     MluI     MscI     NaeI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI    SexAI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     SspI
     StyI     SwaI  Tth111I     XbaI     XhoI     XmaI     XmnI
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