Restriction map for pF1KE2369
 (Linear) (Six-base) MAPSORT of: KIEE2369.seq  Check: 4662  from: 1  to: 1740



KIEE2369.orf

 With 182 enzymes: SgfI * 

                        August 18, 2011 15:44  ..

Acc65I G'GTAC_C

Cuts at:      0    1004    1095    1740 
   Size:       1004      91     645

  Fragments arranged by size: 

                1004     645      91
 
AflII C'TTAA_G

Cuts at:      0     792    1740 
   Size:        792     948
 
AflIII A'CryG_T

Cuts at:      0     895    1215    1740 
   Size:        895     320     525

  Fragments arranged by size: 

                 895     525     320
 
AhdI GACnn_n'nnGTC

Cuts at:      0     489    1740 
   Size:        489    1251
 
AleI CACnn'nnGTG

Cuts at:      0    1438    1740 
   Size:       1438     302
 
AlwNI CAG_nnn'CTG

Cuts at:      0      99     157     937    1740 
   Size:         99      58     780     803

  Fragments arranged by size: 

                 803     780      99      58
 
ApaLI G'TGCA_C

Cuts at:      0    1219    1740 
   Size:       1219     521
 
ApoI r'AATT_y

Cuts at:      0    1363    1740 
   Size:       1363     377
 
AscI GG'CGCG_CC

Cuts at:      0     375    1740 
   Size:        375    1365
 
AvaI C'yCGr_G

Cuts at:      0     193     214    1740 
   Size:        193      21    1526

  Fragments arranged by size: 

                1526     193      21
 
BanI G'GyrC_C

Cuts at:      0     319     475    1004    1095    1740 
   Size:        319     156     529      91     645

  Fragments arranged by size: 

                 645     529     319     156      91
 
BanII G_rGCy'C

Cuts at:      0    1583    1740 
   Size:       1583     157
 
BbeI G_GCGC'C

Cuts at:      0     479    1740 
   Size:        479    1261
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     916    1511    1740 
   Size:        916     595     229

  Fragments arranged by size: 

                 916     595     229
 
BbvCI CC'TCA_GC

Cuts at:      0     186    1740 
   Size:        186    1554
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     525     559    1740 
   Size:        525      34    1181

  Fragments arranged by size: 

                1181     525      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1230    1740 
   Size:       1230     510
 
BclI T'GATC_A

Cuts at:      0     554    1011    1740 
   Size:        554     457     729

  Fragments arranged by size: 

                 729     554     457
 
BlpI GC'TnA_GC

Cuts at:      0      40     403    1667    1740 
   Size:         40     363    1264      73

  Fragments arranged by size: 

                1264     363      73      40
 
Bme1580I G_kGCm'C

Cuts at:      0     305    1223    1740 
   Size:        305     918     517

  Fragments arranged by size: 

                 918     517     305
 
BmgBI CAC'GTC

Cuts at:      0    1653    1740 
   Size:       1653      87
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      99    1740 
   Size:         99    1641
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     636    1740 
   Size:        293     343    1104

  Fragments arranged by size: 

                1104     343     293
 
Bpu10I CC'TnA_GC

Cuts at:      0     186     504    1740 
   Size:        186     318    1236

  Fragments arranged by size: 

                1236     318     186
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      69    1740 
   Size:         69    1671
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     451    1740 
   Size:        451    1289
 
BsaAI yAC'GTr

Cuts at:      0    1218    1740 
   Size:       1218     522
 
BsaHI Gr'CG_yC

Cuts at:      0      26     476     490    1740 
   Size:         26     450      14    1250

  Fragments arranged by size: 

                1250     450      26      14
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     211     241    1740 
   Size:        211      30    1499

  Fragments arranged by size: 

                1499     211      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     137     290     843    1740 
   Size:        137     153     553     897

  Fragments arranged by size: 

                 897     553     153     137
 
BseYI C'CCAG_C

Cuts at:      0     363    1740 
   Size:        363    1377
 
BsiEI CG_ry'CG

Cuts at:      0      37    1740 
   Size:         37    1703
 
BsiHKAI G_wGCw'C

Cuts at:      0     221     873    1223    1740 
   Size:        221     652     350     517

  Fragments arranged by size: 

                 652     517     350     221
 
Bsp1286I G_dGCh'C

Cuts at:      0     221     305     873    1223    1583    1740 
   Size:        221      84     568     350     360     157

  Fragments arranged by size: 

                 568     360     350     221     157      84
 
BspHI T'CATG_A

Cuts at:      0    1229    1740 
   Size:       1229     511
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0       2    1740 
   Size:          2    1738
 
BsrBI CCG'CTC

Cuts at:      0      39     299    1671    1740 
   Size:         39     260    1372      69

  Fragments arranged by size: 

                1372     260      69      39
 
BsrFI r'CCGG_y

Cuts at:      0     253     371    1740 
   Size:        253     118    1369

  Fragments arranged by size: 

                1369     253     118
 
BssHII G'CGCG_C

Cuts at:      0     375    1740 
   Size:        375    1365
 
BssSI C'ACGA_G

Cuts at:      0      73    1740 
   Size:         73    1667
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      99     157    1740 
   Size:         99      58    1583

  Fragments arranged by size: 

                1583      99      58
 
BstBI TT'CG_AA

Cuts at:      0     386    1740 
   Size:        386    1354
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     237    1740 
   Size:        237    1503
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1060    1740 
   Size:       1060     680
 
BtsI GCAGTG_nn'

Cuts at:      0     321    1740 
   Size:        321    1419
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     465    1651    1740 
   Size:        465    1186      89

  Fragments arranged by size: 

                1186     465      89
 
EaeI y'GGCC_r

Cuts at:      0      34    1740 
   Size:         34    1706
 
EagI C'GGCC_G

Cuts at:      0      34    1740 
   Size:         34    1706
 
EarI CTCTTCn'nnn_

Cuts at:      0    1510    1710    1740 
   Size:       1510     200      30

  Fragments arranged by size: 

                1510     200      30
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     275    1740 
   Size:        275    1465
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     636    1740 
   Size:        293     343    1104

  Fragments arranged by size: 

                1104     343     293
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1686    1740 
   Size:       1686      54
 
EcoRV GAT'ATC

Cuts at:      0    1058    1740 
   Size:       1058     682
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     903    1740 
   Size:        903     837
 
HaeII r_GCGC'y

Cuts at:      0     244     479    1740 
   Size:        244     235    1261

  Fragments arranged by size: 

                1261     244     235
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     475     507     838     870    1224    1256    1740 
   Size:        475      32     331      32     354      32     484

  Fragments arranged by size: 

                 484     475     354     331      32      32      32
 
KasI G'GCGC_C

Cuts at:      0     475    1740 
   Size:        475    1265
 
KpnI G_GTAC'C

Cuts at:      0    1008    1099    1740 
   Size:       1008      91     641

  Fragments arranged by size: 

                1008     641      91
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      99     254    1740 
   Size:         99     155    1486

  Fragments arranged by size: 

                1486     155      99
 
MslI CAynn'nnrTG

Cuts at:      0     708    1438    1740 
   Size:        708     730     302

  Fragments arranged by size: 

                 730     708     302
 
MspA1I CmG'CkG

Cuts at:      0       8     157     175     757    1245    1270    1740 
   Size:          8     149      18     582     488      25     470

  Fragments arranged by size: 

                 582     488     470     149      25      18       8
 
NarI GG'CG_CC

Cuts at:      0     476    1740 
   Size:        476    1264
 
NotI GC'GGCC_GC

Cuts at:      0      34    1740 
   Size:         34    1706
 
NspI r_CATG'y

Cuts at:      0     432    1740 
   Size:        432    1308
 
PasI CC'CwG_GG

Cuts at:      0    1576    1740 
   Size:       1576     164
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      78    1740 
   Size:         78    1662
 
PfoI T'CCnGG_A

Cuts at:      0    1687    1740 
   Size:       1687      53
 
PmlI CAC'GTG

Cuts at:      0    1218    1740 
   Size:       1218     522
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     340     372    1740 
   Size:        340      32    1368

  Fragments arranged by size: 

                1368     340      32
 
PstI C_TGCA'G

Cuts at:      0      13     104     153    1740 
   Size:         13      91      49    1587

  Fragments arranged by size: 

                1587      91      49      13
 
PvuII CAG'CTG

Cuts at:      0       8    1245    1270    1740 
   Size:          8    1237      25     470

  Fragments arranged by size: 

                1237     470      25       8
 
SfcI C'TryA_G

Cuts at:      0       9     100     149    1175    1740 
   Size:          9      91      49    1026     565

  Fragments arranged by size: 

                1026     565      91      49       9
 
SfoI GGC'GCC

Cuts at:      0     477    1740 
   Size:        477    1263
 
SgrAI Cr'CCGG_yG

Cuts at:      0     371    1740 
   Size:        371    1369
 
SmlI C'TyrA_G

Cuts at:      0      48     792    1740 
   Size:         48     744     948

  Fragments arranged by size: 

                 948     744      48
 
StyI C'CwwG_G

Cuts at:      0    1038    1740 
   Size:       1038     702
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     475     538    1135    1740 
   Size:        475      63     597     605

  Fragments arranged by size: 

                 605     597     475      63
 
TatI w'GTAC_w

Cuts at:      0     939    1278    1740 
   Size:        939     339     462

  Fragments arranged by size: 

                 939     462     339
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     211     243    1740 
   Size:        211      32    1497

  Fragments arranged by size: 

                1497     211      32
 
XbaI T'CTAG_A

Cuts at:      0     856    1740 
   Size:        856     884
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     759    1573    1740 
   Size:        759     814     167

  Fragments arranged by size: 

                 814     759     167
 

 Enzymes that do cut:

   Acc65I    AflII   AflIII     AhdI     AleI    AlwNI    ApaLI     ApoI
     AscI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI     BlpI Bme1580I    BmgBI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI
   BstAPI    BstBI    BstXI    BtgZI     BtsI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoNI    EcoRV     FalI    HaeII    Hin4I
     KasI     KpnI     MmeI     MslI   MspA1I     NarI     NotI     NspI
     PasI    PflMI     PfoI     PmlI     PsrI     PstI    PvuII     SfcI
     SfoI    SgrAI     SmlI     StyI    TaqII     TatI     TstI     XbaI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI     AgeI     AlfI
     AloI     ApaI     AseI    AsiSI    AvrII     BaeI    BamHI    BfrBI
     BglI    BglII     BmrI     BmtI    BsaBI    BsaWI     BsgI    BsiWI
     BsmI    BsmBI    BspEI    BsrDI    BsrGI   BstEII    BstYI  BstZ17I
   Bsu36I     BtgI     ClaI    CspCI     DraI   DraIII  EcoICRI EcoO109I
    EcoRI     FseI     FspI    FspAI   HincII  HindIII     HpaI     MfeI
     MluI     MscI     NaeI     NcoI     NdeI   NgoMIV     NheI     NruI
     NsiI     PacI     PciI     PmeI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SgfI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XhoI
     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com