Restriction map for pF1KE2508
##################################
# NO CUT ENZYMES (  158 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BclI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BspDI       
BspEI       BspLU11I    BspMI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoT14I     FbaI        FseI        FspI        
HaeII       HaeIII      HhaI        Hin1I       HinP1I      
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspI        NspV        
PacI        PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PstI        
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    StuI        StyI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI          270  GTCTAC               GT^[AC][GT]AC
AlwI          489  GGATC                GGATC(4/5)
AlwNI        1214  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       223  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          204  CTCGAG               C^[CT]CG[GA]G
BanII        1237  GAGCCC               G[GA]GC[CT]^C
BciVI         472  GTATCC               GTATCC(6/5)
BcnI         1134  CCGGG                CC^[GC]GG
BmyI         1237  GAGCCC               G[AGT]GC[ACT]^C
BsaI          920  GAGACC               GGTCTC(1/5)
BsaJI         611  CCCAGG               C^C[ACGT][ACGT]GG
BsaWI         413  ACCGGA               [AT]^CCGG[AT]
BsgI         1300  GTGCAG               GTGCAG(16/14)
BsiYI         689  CCCAAGAGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          689  CCCAAGAGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         247  GGGAC                GGGAC(10/14)
BsoBI         204  CTCGAG               C^[CT]CG[GA]G
Bsp1286I     1237  GAGCCC               G[AGT]GC[ACT]^C
BspHI        1044  TCATGA               T^CATGA
BsrBI         694  GAGCGG               CCGCTC(-3/-3)
DpnII         490  GATC                 ^GATC
DraII         247  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO109I      247  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRV         199  GATATC               GAT^ATC
FauI          602  CCCGC                CCCGC(4/6)
HgaI          268  GCGTC                GACGC(5/10)
HincII        939  GTTGAC               GT[CT]^[GA]AC
HindII        939  GTTGAC               GT[CT]^[GA]AC
HphI          286  GGTGA                GGTGA(8/7)
MaeIII       1324  GTCAC                ^GT[ACGT]AC
MboI          490  GATC                 ^GATC
NciI         1134  CCGGG                CC^[GC]GG
NdeII         490  GATC                 ^GATC
PaeR7I        204  CTCGAG               C^TCGAG
PpuMI         247  GGGACCT              [GA]G^G[AT]CC[CT]
RcaI         1044  TCATGA               T^CATGA
Sau3AI        490  GATC                 ^GATC
SmlI          204  CTCGAG               C^T[CT][GA]AG
Tsp45I       1324  GTCAC                ^GT[GC]AC
XhoI          204  CTCGAG               C^TCGAG
XmnI          223  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AciI          603  CCGC                 CCGC(-3/-1)
              696  GCGG                
AseI          327  ATTAAT               AT^TAAT
              399  ATTAAT              
AsnI          327  ATTAAT               AT^TAAT
              399  ATTAAT              
AvaII          96  GGACC                G^G[AT]CC
              248  GGACC               
BfaI          427  CTAG                 C^TAG
             1330  CTAG                
BsmAI         920  GAGAC                GTCTC(1/5)
              786  GTCTC               
Bsp1407I        2  TGTACA               T^GTACA
             1250  TGTACA              
BsrDI         737  GCAATG               GCAATG(2/0)
              986  GCAATG              
BsrGI           2  TGTACA               T^GTACA
             1250  TGTACA              
BstF5I        608  CATCC                GGATG(2/0)
              450  GGATG               
BstNI          30  CCTGG                CC^[AT]GG
              612  CCAGG               
Cac8I         843  GCAGGC               GC[ACGT]^[ACGT]GC
             1162  GCCTGC              
Cfr13I         96  GGACC                G^G[ACGT]CC
              248  GGACC               
EcoRII         30  CCTGG                ^CC[AT]GG
              612  CCAGG               
EcoT22I       280  ATGCAT               ATGCA^T
              763  ATGCAT              
FokI          608  CATCC                GGATG(9/13)
              450  GGATG               
HapII         414  CCGG                 C^CGG
             1134  CCGG                
HpaII         414  CCGG                 C^CGG
             1134  CCGG                
MaeI          427  CTAG                 C^TAG
             1330  CTAG                
MaeII         729  ACGT                 A^CGT
              873  ACGT                
MspA1I        848  CAGCTG               C[AC]G^C[GT]G
              904  CAGCTG              
MspI          414  CCGG                 C^CGG
             1134  CCGG                
MvaI           30  CCTGG                CC^[AT]GG
              612  CCAGG               
NlaIV          96  GGACCC               GG[ACGT]^[ACGT]CC
              247  GGGACC              
NsiI          280  ATGCAT               ATGCA^T
              763  ATGCAT              
PleI          942  GACTC                GAGTC(4/5)
             1312  GACTC               
PshBI         327  ATTAAT               AT^TAAT
              399  ATTAAT              
PvuII         848  CAGCTG               CAG^CTG
              904  CAGCTG              
Sau96I         96  GGACC                G^G[ACGT]CC
              248  GGACC               
SfaNI         178  GATGC                GCATC(5/9)
              265  GATGC               
SspBI           2  TGTACA               T^GTACA
             1250  TGTACA              
TfiI          421  GAATC                G^A[AT]TC
              791  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AfaI        AluI        BbsI        BbvI        BpuAI       
BsrI        BssKI       DdeI        Fnu4HI      HinfI       
ItaI        MboII       MnlI        MseI        MslI        
MwoI        NlaIII      RsaI        ScrFI       SfcI        
SspI        TaqI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     
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