Restriction map for pF1KE9419
 (Linear) (Six-base) MAPSORT of: KIEE9419.seq  Check: 2358  from: 1  to: 1407



KIEE9419.orf

 With 182 enzymes: SgfI * 

                        September 21, 2017 11:26  ..

AatII G_ACGT'C

Cuts at:      0     487    1407 
   Size:        487     920
 
AccI GT'mk_AC

Cuts at:      0    1103    1361    1407 
   Size:       1103     258      46

  Fragments arranged by size: 

                1103     258      46
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     583    1059    1407 
   Size:         57     526     476     348

  Fragments arranged by size: 

                 526     476     348      57
 
AflIII A'CryG_T

Cuts at:      0     119     494    1106    1407 
   Size:        119     375     612     301

  Fragments arranged by size: 

                 612     375     301     119
 
AlwNI CAG_nnn'CTG

Cuts at:      0     629    1290    1407 
   Size:        629     661     117

  Fragments arranged by size: 

                 661     629     117
 
AscI GG'CGCG_CC

Cuts at:      0     176    1407 
   Size:        176    1231
 
AvaI C'yCGr_G

Cuts at:      0     195     233     578    1407 
   Size:        195      38     345     829

  Fragments arranged by size: 

                 829     345     195      38
 
BanI G'GyrC_C

Cuts at:      0    1068    1302    1407 
   Size:       1068     234     105

  Fragments arranged by size: 

                1068     234     105
 
BbeI G_GCGC'C

Cuts at:      0    1306    1407 
   Size:       1306     101
 
BglI GCCn_nnn'nGGC

Cuts at:      0     679    1407 
   Size:        679     728
 
Bme1580I G_kGCm'C

Cuts at:      0    1073    1407 
   Size:       1073     334
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     273     686    1407 
   Size:        273     413     721

  Fragments arranged by size: 

                 721     413     273
 
BmtI G_CTAG'C

Cuts at:      0      64    1407 
   Size:         64    1343
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     181    1062    1407 
   Size:        115      66     881     345

  Fragments arranged by size: 

                 881     345     115      66
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1183    1407 
   Size:       1183     224
 
BsaBI GATnn'nnATC

Cuts at:      0     247    1407 
   Size:        247    1160
 
BsaHI Gr'CG_yC

Cuts at:      0     484     933    1303    1352    1407 
   Size:        484     449     370      49      55

  Fragments arranged by size: 

                 484     449     370      55      49
 
BsaWI w'CCGG_w

Cuts at:      0     135     572     940    1407 
   Size:        135     437     368     467

  Fragments arranged by size: 

                 467     437     368     135
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     745    1407 
   Size:        745     662
 
BseYI C'CCAG_C

Cuts at:      0     153     765     789     819     837    1390    1407 
   Size:        153     612      24      30      18     553      17

  Fragments arranged by size: 

                 612     553     153      30      24      18      17
 
BsiEI CG_ry'CG

Cuts at:      0     591     609     888     941    1319    1407 
   Size:        591      18     279      53     378      88

  Fragments arranged by size: 

                 591     378     279      88      53      18
 
BsiHKAI G_wGCw'C

Cuts at:      0     398    1277    1407 
   Size:        398     879     130

  Fragments arranged by size: 

                 879     398     130
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1229    1407 
   Size:       1229     178
 
Bsp1286I G_dGCh'C

Cuts at:      0     398    1073    1277    1407 
   Size:        398     675     204     130

  Fragments arranged by size: 

                 675     398     204     130
 
BspEI T'CCGG_A

Cuts at:      0     135    1407 
   Size:        135    1272
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     305     869    1340    1391    1407 
   Size:        305     564     471      51      16

  Fragments arranged by size: 

                 564     471     305      51      16
 
BssHII G'CGCG_C

Cuts at:      0     176    1407 
   Size:        176    1231
 
BssSI C'ACGA_G

Cuts at:      0     108    1171    1276    1407 
   Size:        108    1063     105     131

  Fragments arranged by size: 

                1063     131     108     105
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     813    1407 
   Size:        813     594
 
BstEII G'GTnAC_C

Cuts at:      0     969    1407 
   Size:        969     438
 
BstYI r'GATC_y

Cuts at:      0     659    1407 
   Size:        659     748
 
BstZ17I GTA'TAC

Cuts at:      0    1104    1407 
   Size:       1104     303
 
BtgI C'CryG_G

Cuts at:      0     476     710     917    1319    1407 
   Size:        476     234     207     402      88

  Fragments arranged by size: 

                 476     402     234     207      88
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     864    1203    1407 
   Size:        864     339     204

  Fragments arranged by size: 

                 864     339     204
 
BtsI GCAGTG_nn'

Cuts at:      0     518    1407 
   Size:        518     889
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      93     128    1407 
   Size:         93      35    1279

  Fragments arranged by size: 

                1279      93      35
 
EaeI y'GGCC_r

Cuts at:      0       8     101     588     606     638     671    1316    1407 
   Size:          8      93     487      18      32      33     645      91

  Fragments arranged by size: 

                 645     487      93      91      33      32      18       8
            
 
EagI C'GGCC_G

Cuts at:      0     588     606    1316    1407 
   Size:        588      18     710      91

  Fragments arranged by size: 

                 710     588      91      18
 
EarI CTCTTCn'nnn_

Cuts at:      0      76     313     361     559    1407 
   Size:         76     237      48     198     848

  Fragments arranged by size: 

                 848     237     198      76      48
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     115     181     583    1059    1062    1407 
   Size:         57      58      66     402     476       3     345

  Fragments arranged by size: 

                 476     402     345      66      58      57       3
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      94    1096    1407 
   Size:         94    1002     311

  Fragments arranged by size: 

                1002     311      94
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1296    1407 
   Size:       1296     111
 
HaeII r_GCGC'y

Cuts at:      0     376     649     953    1033    1198    1306    1377    1407 
   Size:        376     273     304      80     165     108      71      30

  Fragments arranged by size: 

                 376     304     273     165     108      80      71      30
            
 
KasI G'GCGC_C

Cuts at:      0    1302    1407 
   Size:       1302     105
 
MluI A'CGCG_T

Cuts at:      0     119    1106    1407 
   Size:        119     987     301

  Fragments arranged by size: 

                 987     301     119
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1163    1407 
   Size:       1163     244
 
MslI CAynn'nnrTG

Cuts at:      0     253     493     523     529    1240    1407 
   Size:        253     240      30       6     711     167

  Fragments arranged by size: 

                 711     253     240     167      30       6
 
MspA1I CmG'CkG

Cuts at:      0     203     626     837     919    1145    1321    1367    1407 
   Size:        203     423     211      82     226     176      46      40

  Fragments arranged by size: 

                 423     226     211     203     176      82      46      40
            
 
NarI GG'CG_CC

Cuts at:      0    1303    1407 
   Size:       1303     104
 
NcoI C'CATG_G

Cuts at:      0     476    1407 
   Size:        476     931
 
NheI G'CTAG_C

Cuts at:      0      60    1407 
   Size:         60    1347
 
NotI GC'GGCC_GC

Cuts at:      0    1316    1407 
   Size:       1316      91
 
NspI r_CATG'y

Cuts at:      0     327     498    1245    1407 
   Size:        327     171     747     162

  Fragments arranged by size: 

                 747     327     171     162
 
PasI CC'CwG_GG

Cuts at:      0     279    1407 
   Size:        279    1128
 
PciI A'CATG_T

Cuts at:      0     494    1407 
   Size:        494     913
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     549    1407 
   Size:        549     858
 
PfoI T'CCnGG_A

Cuts at:      0      83     752    1407 
   Size:         83     669     655

  Fragments arranged by size: 

                 669     655      83
 
PstI C_TGCA'G

Cuts at:      0     131    1295    1407 
   Size:        131    1164     112

  Fragments arranged by size: 

                1164     131     112
 
PvuII CAG'CTG

Cuts at:      0     626    1145    1407 
   Size:        626     519     262

  Fragments arranged by size: 

                 626     519     262
 
SacII CC_GC'GG

Cuts at:      0     920    1322    1407 
   Size:        920     402      85

  Fragments arranged by size: 

                 920     402      85
 
SapI GCTCTTCn'nnn_

Cuts at:      0     313     559    1407 
   Size:        313     246     848

  Fragments arranged by size: 

                 848     313     246
 
SfcI C'TryA_G

Cuts at:      0     127    1291    1362    1407 
   Size:        127    1164      71      45

  Fragments arranged by size: 

                1164     127      71      45
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     679    1407 
   Size:        679     728
 
SfoI GGC'GCC

Cuts at:      0    1304    1407 
   Size:       1304     103
 
SmaI CCC'GGG

Cuts at:      0     197     235    1407 
   Size:        197      38    1172

  Fragments arranged by size: 

                1172     197      38
 
SmlI C'TyrA_G

Cuts at:      0    1198    1407 
   Size:       1198     209
 
SphI G_CATG'C

Cuts at:      0     327    1245    1407 
   Size:        327     918     162

  Fragments arranged by size: 

                 918     327     162
 
StyI C'CwwG_G

Cuts at:      0     476     641    1407 
   Size:        476     165     766

  Fragments arranged by size: 

                 766     476     165
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     255    1407 
   Size:        255    1152
 
TatI w'GTAC_w

Cuts at:      0     260    1407 
   Size:        260    1147
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     130     162    1407 
   Size:        130      32    1245

  Fragments arranged by size: 

                1245     130      32
 
XmaI C'CCGG_G

Cuts at:      0     195     233    1407 
   Size:        195      38    1174

  Fragments arranged by size: 

                1174     195      38
 
ZraI GAC'GTC

Cuts at:      0     485    1407 
   Size:        485     922
 

 Enzymes that do cut:

    AatII     AccI     AcuI   AflIII    AlwNI     AscI     AvaI     BanI
     BbeI     BglI Bme1580I     BmrI     BmtI     BpmI    BpuEI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BspMI   BssHII    BssSI   BstAPI   BstEII    BstYI  BstZ17I
     BtgI    BtgZI     BtsI    CspCI     EaeI     EagI     EarI  Eco57MI
    EcoNI EcoO109I    HaeII     KasI     MluI     MmeI     MslI   MspA1I
     NarI     NcoI     NheI     NotI     NspI     PasI     PciI    PflMI
     PfoI     PstI    PvuII    SacII     SapI     SfcI     SfiI     SfoI
     SmaI     SmlI     SphI     StyI    TaqII     TatI     TstI     XmaI
     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     ApoI     AseI    AsiSI    AvrII
     BaeI    BamHI    BanII     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI    BmgBI     BplI   Bpu10I     BsaI    BsaAI
    BsaXI     BsgI    BsiWI     BsmI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI    BstBI    BstXI   Bsu36I     ClaI     DraI   DraIII     DrdI
     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MscI     NaeI
     NdeI   NgoMIV     NruI     NsiI     PacI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI     SalI    SanDI     SbfI     ScaI    SexAI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI
     XcmI     XhoI     XmnI
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