Restriction map for pF1KE1499
 (Linear) (Six-base) MAPSORT of: KIEE1499.seq  Check: 4876  from: 1  to: 9291



KIEE1499.orf

 With 182 enzymes: SgfI * 

                        August 2, 2010 17:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1719    2154    2741    2886    9291 
   Size:       1719     435     587     145    6405

  Fragments arranged by size: 

                6405    1719     587     435     145
 
AatII G_ACGT'C

Cuts at:      0    2534    3248    4933    9291 
   Size:       2534     714    1685    4358

  Fragments arranged by size: 

                4358    2534    1685     714
 
AccI GT'mk_AC

Cuts at:      0    1412    2615    3513    7715    9291 
   Size:       1412    1203     898    4202    1576

  Fragments arranged by size: 

                4202    1576    1412    1203     898
 
Acc65I G'GTAC_C

Cuts at:      0    8711    9291 
   Size:       8711     580
 
AclI AA'CG_TT

Cuts at:      0     265    9291 
   Size:        265    9026
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     445     694     808     832    1021    1123    3232    3731 
   Size:        445     249     114      24     189     102    2109     499

Cuts at:   3731    4676    4810    4822    6352    8251    8684    9291 
   Size:        945     134      12    1530    1899     433     607

  Fragments arranged by size: 

                2109    1899    1530     945     607     499     445     433
                 249     189     134     114     102      24      12
 
AfeI AGC'GCT

Cuts at:      0    2134    9291 
   Size:       2134    7157
 
AflII C'TTAA_G

Cuts at:      0    4711    9291 
   Size:       4711    4580
 
AflIII A'CryG_T

Cuts at:      0    1178    1334    2012    2090    4638    6167    7760    8083 
   Size:       1178     156     678      78    2548    1529    1593     323

Cuts at:   8083    8270    9185    9291 
   Size:        187     915     106

  Fragments arranged by size: 

                2548    1593    1529    1178     915     678     323     187
                 156     106      78
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2406    7095    9291 
   Size:       2406    4689    2196

  Fragments arranged by size: 

                4689    2406    2196
 
AleI CACnn'nnGTG

Cuts at:      0    2035    7690    7841    9291 
   Size:       2035    5655     151    1450

  Fragments arranged by size: 

                5655    2035    1450     151
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3653    5332    7450    9291 
   Size:       3653    1679    2118    1841

  Fragments arranged by size: 

                3653    2118    1841    1679
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    7715    7747    8804    8836    8890    8922    9291 
   Size:       7715      32    1057      32      54      32     369

  Fragments arranged by size: 

                7715    1057     369      54      32      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     843     972    1579    1695    2289    2640    2757    6977 
   Size:        843     129     607     116     594     351     117    4220

Cuts at:   6977    8856    8881    9147    9291 
   Size:       1879      25     266     144

  Fragments arranged by size: 

                4220    1879     843     607     594     351     266     144
                 129     117     116      25
 
ApaI G_GGCC'C

Cuts at:      0    3145    5280    5296    6939    7121    8161    9291 
   Size:       3145    2135      16    1643     182    1040    1130

  Fragments arranged by size: 

                3145    2135    1643    1130    1040     182      16
 
ApaLI G'TGCA_C

Cuts at:      0    1600    9291 
   Size:       1600    7691
 
ApoI r'AATT_y

Cuts at:      0     526     532    4045    4660    6703    6803    7736    8056 
   Size:        526       6    3513     615    2043     100     933     320

Cuts at:   8056    8869    9291 
   Size:        813     422

  Fragments arranged by size: 

                3513    2043     933     813     615     526     422     320
                 100       6
 
AvaI C'yCGr_G

Cuts at:      0      75    2404    2627    3079    5547    7071    7114    7147 
   Size:         75    2329     223     452    2468    1524      43      33

Cuts at:   7147    7275    7346    7506    8893    9291 
   Size:        128      71     160    1387     398

  Fragments arranged by size: 

                2468    2329    1524    1387     452     398     223     160
                 128      75      71      43      33
 
AvrII C'CTAG_G

Cuts at:      0    8328    9291 
   Size:       8328     963
 
BamHI G'GATC_C

Cuts at:      0    2765    3458    6133    6785    7019    9291 
   Size:       2765     693    2675     652     234    2272

  Fragments arranged by size: 

                2765    2675    2272     693     652     234
 
BanI G'GyrC_C

Cuts at:      0      91    6985    7261    7324    8711    9291 
   Size:         91    6894     276      63    1387     580

  Fragments arranged by size: 

                6894    1387     580     276      91      63
 
BanII G_rGCy'C

Cuts at:      0     759    2321    2351    3145    3508    3533    3899    4800 
   Size:        759    1562      30     794     363      25     366     901

Cuts at:   4800    4811    5280    5287    5296    5726    6939    7121    7252 
   Size:         11     469       7       9     430    1213     182     131

Cuts at:   7252    7288    7415    7450    7462    8161    9291 
   Size:         36     127      35      12     699    1130

  Fragments arranged by size: 

                1562    1213    1130     901     794     759     699     469
                 430     366     363     182     131     127      36      35
                  30      25      12      11       9       7
 
BbeI G_GCGC'C

Cuts at:      0    6989    7265    9291 
   Size:       6989     276    2026

  Fragments arranged by size: 

                6989    2026     276
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     668    1964    3119    3240    5913    9291 
   Size:        668    1296    1155     121    2673    3378

  Fragments arranged by size: 

                3378    2673    1296    1155     668     121
 
BbvCI CC'TCA_GC

Cuts at:      0    1024    4408    7805    9291 
   Size:       1024    3384    3397    1486

  Fragments arranged by size: 

                3397    3384    1486    1024
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2077    2111    3060    3094    5443    5477    9291 
   Size:       2077      34     949      34    2349      34    3814

  Fragments arranged by size: 

                3814    2349    2077     949      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     848    3811    3939    6363    7557    8913    9291 
   Size:        848    2963     128    2424    1194    1356     378

  Fragments arranged by size: 

                2963    2424    1356    1194     848     378     128
 
BclI T'GATC_A

Cuts at:      0     470    2180    3176    6290    8439    9291 
   Size:        470    1710     996    3114    2149     852

  Fragments arranged by size: 

                3114    2149    1710     996     852     470
 
BglI GCCn_nnn'nGGC

Cuts at:      0      58      67    3445    3640    6962    7150    7173    7490 
   Size:         58       9    3378     195    3322     188      23     317

Cuts at:   7490    9291 
   Size:       1801

  Fragments arranged by size: 

                3378    3322    1801     317     195     188      58      23
                   9
 
BglII A'GATC_T

Cuts at:      0    2457    2550    6282    9291 
   Size:       2457      93    3732    3009

  Fragments arranged by size: 

                3732    3009    2457      93
 
BlpI GC'TnA_GC

Cuts at:      0    3893    4372    5350    8399    9291 
   Size:       3893     479     978    3049     892

  Fragments arranged by size: 

                3893    3049     978     892     479
 
Bme1580I G_kGCm'C

Cuts at:      0    1604    3145    5280    5296    5375    5603    6939    7121 
   Size:       1604    1541    2135      16      79     228    1336     182

Cuts at:   7121    7327    7798    8161    9291 
   Size:        206     471     363    1130

  Fragments arranged by size: 

                2135    1604    1541    1336    1130     471     363     228
                 206     182      79      16
 
BmgBI CAC'GTC

Cuts at:      0    2054    2091    4322    6732    9188    9291 
   Size:       2054      37    2231    2410    2456     103

  Fragments arranged by size: 

                2456    2410    2231    2054     103      37
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1919    6935    8866    9291 
   Size:       1919    5016    1931     425

  Fragments arranged by size: 

                5016    1931    1919     425
 
BmtI G_CTAG'C

Cuts at:      0    7319    9291 
   Size:       7319    1972
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     721    1297    1519    1672    2080    2214    2365    2467 
   Size:        721     576     222     153     408     134     151     102

Cuts at:   2467    2665    2728    2821    3211    3492    3760    4381    5077 
   Size:        198      63      93     390     281     268     621     696

Cuts at:   5077    5145    5202    5242    5626    5788    6694    7264    7368 
   Size:         68      57      40     384     162     906     570     104

Cuts at:   7368    7476    7510    8524    9118    9291 
   Size:        108      34    1014     594     173

  Fragments arranged by size: 

                1014     906     721     696     621     594     576     570
                 408     390     384     281     268     222     198     173
                 162     153     151     134     108     104     102      93
                  68      63      57      40      34
 
Bpu10I CC'TnA_GC

Cuts at:      0     894    1024    2212    4408    5515    7805    9291 
   Size:        894     130    1188    2196    1107    2290    1486

  Fragments arranged by size: 

                2290    2196    1486    1188    1107     894     130
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2986    3004    6328    7861    9291 
   Size:       2986      18    3324    1533    1430

  Fragments arranged by size: 

                3324    2986    1533    1430      18
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     555    3920    4643    6733    7062    8092    8572    8989 
   Size:        555    3365     723    2090     329    1030     480     417

Cuts at:   8989    9291 
   Size:        302

  Fragments arranged by size: 

                3365    2090    1030     723     555     480     417     329
                 302
 
BsaAI yAC'GTr

Cuts at:      0    3165    3420    3950    5376    7583    8084    8273    8924 
   Size:       3165     255     530    1426    2207     501     189     651

Cuts at:   8924    8949    9291 
   Size:         25     342

  Fragments arranged by size: 

                3165    2207    1426     651     530     501     342     255
                 189      25
 
BsaBI GATnn'nnATC

Cuts at:      0     643    4915    9291 
   Size:        643    4272    4376

  Fragments arranged by size: 

                4376    4272     643
 
BsaHI Gr'CG_yC

Cuts at:      0    2381    2531    2977    3169    3245    4930    5228    5432 
   Size:       2381     150     446     192      76    1685     298     204

Cuts at:   5432    5594    6986    7193    7262    7292    9176    9291 
   Size:        162    1392     207      69      30    1884     115

  Fragments arranged by size: 

                2381    1884    1685    1392     446     298     207     204
                 192     162     150     115      76      69      30
 
BsaWI w'CCGG_w

Cuts at:      0    1961    8708    9291 
   Size:       1961    6747     583

  Fragments arranged by size: 

                6747    1961     583
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1270    1300    1324    1354    2239    2269    2584    2614 
   Size:       1270      30      24      30     885      30     315      30

Cuts at:   2614    5194    5224    7504    7534    7715    7745    8185    8215 
   Size:       2580      30    2280      30     181      30     440      30

Cuts at:   8215    8362    8392    8804    8834    8890    8920    9064    9094 
   Size:        147      30     412      30      56      30     144      30

Cuts at:   9094    9291 
   Size:        197

  Fragments arranged by size: 

                2580    2280    1270     885     440     412     315     197
                 181     147     144      56      30      30      30      30
                  30      30      30      30      30      30      30      30
                  24
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     124     364    2077    2329    3447    3562    5506    5532 
   Size:        124     240    1713     252    1118     115    1944      26

Cuts at:   5532    5535    5596    5629    5847    7050    7578    8554    9291 
   Size:          3      61      33     218    1203     528     976     737

  Fragments arranged by size: 

                1944    1713    1203    1118     976     737     528     252
                 240     218     124     115      61      33      26       3
            
 
BseYI C'CCAG_C

Cuts at:      0     343    2757    3138    5112    5779    6970    6991    7025 
   Size:        343    2414     381    1974     667    1191      21      34

Cuts at:   7025    7159    7168    7423    9291 
   Size:        134       9     255    1868

  Fragments arranged by size: 

                2414    1974    1868    1191     667     381     343     255
                 134      34      21       9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     937     958    1618    2137    2740    2920    3247    5735 
   Size:        937      21     660     519     603     180     327    2488

Cuts at:   5735    6322    7630    7865    8701    9291 
   Size:        587    1308     235     836     590

  Fragments arranged by size: 

                2488    1308     937     836     660     603     590     587
                 519     327     235     180      21
 
BsiEI CG_ry'CG

Cuts at:      0    5065    5305    5489    9291 
   Size:       5065     240     184    3802

  Fragments arranged by size: 

                5065    3802     240     184
 
BsiHKAI G_wGCw'C

Cuts at:      0    1324    1604    2321    2351    2849    3533    3545    3899 
   Size:       1324     280     717      30     498     684      12     354

Cuts at:   3899    5287    5726    6209    7462    7582    7588    8942    9291 
   Size:       1388     439     483    1253     120       6    1354     349

  Fragments arranged by size: 

                1388    1354    1324    1253     717     684     498     483
                 439     354     349     280     120      30      12       6
            
 
BsmI GAATG_Cn'

Cuts at:      0    3205    3243    6753    9291 
   Size:       3205      38    3510    2538

  Fragments arranged by size: 

                3510    3205    2538      38
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2266    2984    3148    3387    4880    4937    5220    5476 
   Size:       2266     718     164     239    1493      57     283     256

Cuts at:   5476    9291 
   Size:       3815

  Fragments arranged by size: 

                3815    2266    1493     718     283     256     239     164
                  57
 
Bsp1286I G_dGCh'C

Cuts at:      0     759    1324    1604    2321    2351    2849    3145    3508 
   Size:        759     565     280     717      30     498     296     363

Cuts at:   3508    3533    3545    3899    4800    4811    5280    5287    5296 
   Size:         25      12     354     901      11     469       7       9

Cuts at:   5296    5375    5603    5726    6209    6939    7121    7252    7288 
   Size:         79     228     123     483     730     182     131      36

Cuts at:   7288    7327    7415    7450    7462    7582    7588    7798    8161 
   Size:         39      88      35      12     120       6     210     363

Cuts at:   8161    8942    9291 
   Size:        781     349

  Fragments arranged by size: 

                 901     781     759     730     717     565     498     483
                 469     363     363     354     349     296     280     228
                 210     182     131     123     120      88      79      39
                  36      35      30      25      12      12      11       9
                   7       6
 
BspHI T'CATG_A

Cuts at:      0     285    1827    2655    3837    6311    9291 
   Size:        285    1542     828    1182    2474    2980

  Fragments arranged by size: 

                2980    2474    1542    1182     828     285
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     917    1719    2154    2648    2741    2886    3054    3269 
   Size:        917     802     435     494      93     145     168     215

Cuts at:   3269    8723    9007    9291 
   Size:       5454     284     284

  Fragments arranged by size: 

                5454     917     802     494     435     284     284     215
                 168     145      93
 
BsrBI CCG'CTC

Cuts at:      0       5    1317    4776    4831    5061    5108    7380    9213 
   Size:          5    1312    3459      55     230      47    2272    1833

Cuts at:   9213    9291 
   Size:         78

  Fragments arranged by size: 

                3459    2272    1833    1312     230      78      55      47
                   5
 
BsrDI GCAATG_nn'

Cuts at:      0     681     906    1813    5229    9291 
   Size:        681     225     907    3416    4062

  Fragments arranged by size: 

                4062    3416     907     681     225
 
BsrFI r'CCGG_y

Cuts at:      0      94     947    5434    7007    7153    9154    9291 
   Size:         94     853    4487    1573     146    2001     137

  Fragments arranged by size: 

                4487    2001    1573     853     146     137      94
 
BsrGI T'GTAC_A

Cuts at:      0    6170    9291 
   Size:       6170    3121
 
BssHII G'CGCG_C

Cuts at:      0    2888    7163    7309    7311    9291 
   Size:       2888    4275     146       2    1980

  Fragments arranged by size: 

                4275    2888    1980     146       2
 
BssSI C'ACGA_G

Cuts at:      0    1624    2848    3544    5746    6151    6996    9291 
   Size:       1624    1224     696    2202     405     845    2295

  Fragments arranged by size: 

                2295    2202    1624    1224     845     696     405
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     972    2718    7608    8550    9147    9291 
   Size:        972    1746    4890     942     597     144

  Fragments arranged by size: 

                4890    1746     972     942     597     144
 
BstBI TT'CG_AA

Cuts at:      0     162    4503    9291 
   Size:        162    4341    4788

  Fragments arranged by size: 

                4788    4341     162
 
BstEII G'GTnAC_C

Cuts at:      0    8355    8508    9291 
   Size:       8355     153     783

  Fragments arranged by size: 

                8355     783     153
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     126     942    1221    2584    3469    3975    7668    8521 
   Size:        126     816     279    1363     885     506    3693     853

Cuts at:   8521    8799    9291 
   Size:        278     492

  Fragments arranged by size: 

                3693    1363     885     853     816     506     492     279
                 278     126
 
BstYI r'GATC_y

Cuts at:      0     306     428    2000    2457    2550    2765    3458    4869 
   Size:        306     122    1572     457      93     215     693    1411

Cuts at:   4869    6117    6133    6282    6785    7019    9291 
   Size:       1248      16     149     503     234    2272

  Fragments arranged by size: 

                2272    1572    1411    1248     693     503     457     306
                 234     215     149     122      93      16
 
BstZ17I GTA'TAC

Cuts at:      0    3514    9291 
   Size:       3514    5777
 
Bsu36I CC'TnA_GG

Cuts at:      0     740    8320    9291 
   Size:        740    7580     971

  Fragments arranged by size: 

                7580     971     740
 
BtgI C'CryG_G

Cuts at:      0      53    1130    1752    3500    5045    5246    5615    8249 
   Size:         53    1077     622    1748    1545     201     369    2634

Cuts at:   8249    9291 
   Size:       1042

  Fragments arranged by size: 

                2634    1748    1545    1077    1042     622     369     201
                  53
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     120    4902    5136    6827    8262    8661    9291 
   Size:        120    4782     234    1691    1435     399     630

  Fragments arranged by size: 

                4782    1691    1435     630     399     234     120
 
BtsI GCAGTG_nn'

Cuts at:      0     870    2719    7697    8468    9148    9291 
   Size:        870    1849    4978     771     680     143

  Fragments arranged by size: 

                4978    1849     870     771     680     143
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    8457    8492    9291 
   Size:       8457      35     799

  Fragments arranged by size: 

                8457     799      35
 
DraI TTT'AAA

Cuts at:      0    4307    5900    6830    9048    9291 
   Size:       4307    1593     930    2218     243

  Fragments arranged by size: 

                4307    2218    1593     930     243
 
DraIII CAC_nnn'GTG

Cuts at:      0    1629    5713    9291 
   Size:       1629    4084    3578

  Fragments arranged by size: 

                4084    3578    1629
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    5485    9291 
   Size:       5485    3806
 
EaeI y'GGCC_r

Cuts at:      0      59     116     128    1211    1233    2139    5062    5302 
   Size:         59      57      12    1083      22     906    2923     240

Cuts at:   5302    5465    5618    6261    6954    7592    7742    8127    8312 
   Size:        163     153     643     693     638     150     385     185

Cuts at:   8312    8465    9291 
   Size:        153     826

  Fragments arranged by size: 

                2923    1083     906     826     693     643     638     385
                 240     185     163     153     153     150      59      57
                  22      12
 
EagI C'GGCC_G

Cuts at:      0    5062    5302    9291 
   Size:       5062     240    3989

  Fragments arranged by size: 

                5062    3989     240
 
EarI CTCTTCn'nnn_

Cuts at:      0    1802    1922    2326    2356    3352    3385    3845    3974 
   Size:       1802     120     404      30     996      33     460     129

Cuts at:   3974    4531    5398    5564    5676    5856    8987    9291 
   Size:        557     867     166     112     180    3131     304

  Fragments arranged by size: 

                3131    1802     996     867     557     460     404     304
                 180     166     129     120     112      33      30
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      32    3145    5677    9291 
   Size:         32    3113    2532    3614

  Fragments arranged by size: 

                3614    3113    2532      32
 
EcoICRI GAG'CTC

Cuts at:      0    2319    2349    3531    3897    5285    5724    7460    9291 
   Size:       2319      30    1182     366    1388     439    1736    1831

  Fragments arranged by size: 

                2319    1831    1736    1388    1182     439     366      30
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     445     694     721     808     832    1021    1123    1297 
   Size:        445     249      27      87      24     189     102     174

Cuts at:   1297    1519    1672    2080    2214    2365    2467    2665    2728 
   Size:        222     153     408     134     151     102     198      63

Cuts at:   2728    2821    3211    3232    3492    3731    3760    4381    4676 
   Size:         93     390      21     260     239      29     621     295

Cuts at:   4676    4810    4822    5077    5145    5202    5242    5626    5788 
   Size:        134      12     255      68      57      40     384     162

Cuts at:   5788    6352    6694    7264    7368    7476    7510    8251    8524 
   Size:        564     342     570     104     108      34     741     273

Cuts at:   8524    8684    9118    9291 
   Size:        160     434     173

  Fragments arranged by size: 

                 741     621     570     564     445     434     408     390
                 384     342     295     273     260     255     249     239
                 222     198     189     174     173     162     160     153
                 151     134     134     108     104     102     102      93
                  87      68      63      57      40      34      29      27
                  24      21      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3091    7438    8218    8689    9091    9094    9291 
   Size:       3091    4347     780     471     402       3     197

  Fragments arranged by size: 

                4347    3091     780     471     402     197       3
 
EcoO109I rG'GnC_Cy

Cuts at:      0     123     253     807     819    1434    2121    2196    2637 
   Size:        123     130     554      12     615     687      75     441

Cuts at:   2637    3716    4205    4485    5276    5293    5511    5958    6935 
   Size:       1079     489     280     791      17     218     447     977

Cuts at:   6935    6936    7118    8157    8158    8325    9099    9291 
   Size:          1     182    1039       1     167     774     192

  Fragments arranged by size: 

                1079    1039     977     791     774     687     615     554
                 489     447     441     280     218     192     182     167
                 130     123      75      17      12       1       1
 
EcoRI G'AATT_C

Cuts at:      0    8056    8869    9291 
   Size:       8056     813     422

  Fragments arranged by size: 

                8056     813     422
 
EcoRV GAT'ATC

Cuts at:      0    3786    9291 
   Size:       3786    5505
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     384    6633    7431    9291 
   Size:        384    6249     798    1860

  Fragments arranged by size: 

                6249    1860     798     384
 
FseI GG_CCGG'CC

Cuts at:      0    7157    9291 
   Size:       7157    2134
 
FspI TGC'GCA

Cuts at:      0    2365    5320    9291 
   Size:       2365    2955    3971

  Fragments arranged by size: 

                3971    2955    2365
 
HaeII r_GCGC'y

Cuts at:      0    2136    2481    5429    6989    7265    9291 
   Size:       2136     345    2948    1560     276    2026

  Fragments arranged by size: 

                2948    2136    2026    1560     345     276
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1254    1286    1324    1356    1405    1437    1501    1533 
   Size:       1254      32      38      32      49      32      64      32

Cuts at:   1533    2287    2319    2380    2392    2412    2424    2997    3029 
   Size:        754      32      61      12      20      12     573      32

Cuts at:   3029    3756    3788    4442    4474    4945    4977    5194    5226 
   Size:        727      32     654      32     471      32     217      32

Cuts at:   5226    5422    5454    5653    5685    7081    7113    7504    7536 
   Size:        196      32     199      32    1396      32     391      32

Cuts at:   7536    8105    8137    8408    8440    9291 
   Size:        569      32     271      32     851

  Fragments arranged by size: 

                1396    1254     851     754     727     654     573     569
                 471     391     271     217     199     196      64      61
                  49      38      32      32      32      32      32      32
                  32      32      32      32      32      32      32      32
                  32      32      20      12      12
 
HincII GTy'rAC

Cuts at:      0    2175    3030    5796    7886    9291 
   Size:       2175     855    2766    2090    1405

  Fragments arranged by size: 

                2766    2175    2090    1405     855
 
HindIII A'AGCT_T

Cuts at:      0     870    1397    8017    9291 
   Size:        870     527    6620    1274

  Fragments arranged by size: 

                6620    1274     870     527
 
KasI G'GCGC_C

Cuts at:      0    6985    7261    9291 
   Size:       6985     276    2030

  Fragments arranged by size: 

                6985    2030     276
 
KpnI G_GTAC'C

Cuts at:      0    8715    9291 
   Size:       8715     576
 
MfeI C'AATT_G

Cuts at:      0    5735    9291 
   Size:       5735    3556
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2743    3375    3779    4086    5055    5477    7059    8913 
   Size:       2743     632     404     307     969     422    1582    1854

Cuts at:   8913    9291 
   Size:        378

  Fragments arranged by size: 

                2743    1854    1582     969     632     422     404     378
                 307
 
MscI TGG'CCA

Cuts at:      0    1213    1235    2141    5620    6263    7744    8467    9291 
   Size:       1213      22     906    3479     643    1481     723     824

  Fragments arranged by size: 

                3479    1481    1213     906     824     723     643      22
            
 
MslI CAynn'nnrTG

Cuts at:      0     460     685    1124    1207    1781    2017    2033    2035 
   Size:        460     225     439      83     574     236      16       2

Cuts at:   2035    2947    3467    3635    5176    7669    7690    7841    9227 
   Size:        912     520     168    1541    2493      21     151    1386

Cuts at:   9227    9291 
   Size:         64

  Fragments arranged by size: 

                2493    1541    1386     912     574     520     460     439
                 236     225     168     151      83      64      21      16
                   2
 
MspA1I CmG'CkG

Cuts at:      0      14      55     786    1023    1275    1539    1644    1650 
   Size:         14      41     731     237     252     264     105       6

Cuts at:   1650    1701    1899    1953    2289    2361    2724    3603    4185 
   Size:         51     198      54     336      72     363     879     582

Cuts at:   4185    5116    5357    5522    6324    6872    6896    6905    6923 
   Size:        931     241     165     802     548      24       9      18

Cuts at:   6923    6953    6977    7368    7530    7804    9291 
   Size:         30      24     391     162     274    1487

  Fragments arranged by size: 

                1487     931     879     802     731     582     548     391
                 363     336     274     264     252     241     237     198
                 165     162     105      72      54      51      41      30
                  24      24      18      14       9       6
 
NaeI GCC'GGC

Cuts at:      0      96    5436    7009    7155    9156    9291 
   Size:         96    5340    1573     146    2001     135

  Fragments arranged by size: 

                5340    2001    1573     146     135      96
 
NarI GG'CG_CC

Cuts at:      0    6986    7262    9291 
   Size:       6986     276    2029

  Fragments arranged by size: 

                6986    2029     276
 
NcoI C'CATG_G

Cuts at:      0    1130    5615    9291 
   Size:       1130    4485    3676

  Fragments arranged by size: 

                4485    3676    1130
 
NgoMIV G'CCGG_C

Cuts at:      0      94    5434    7007    7153    9154    9291 
   Size:         94    5340    1573     146    2001     137

  Fragments arranged by size: 

                5340    2001    1573     146     137      94
 
NheI G'CTAG_C

Cuts at:      0    7315    9291 
   Size:       7315    1976
 
NotI GC'GGCC_GC

Cuts at:      0    5062    9291 
   Size:       5062    4229
 
NruI TCG'CGA

Cuts at:      0    1267    9291 
   Size:       1267    8024
 
NspI r_CATG'y

Cuts at:      0     390     690     991    1338    2016    2866    3285    3634 
   Size:        390     300     301     347     678     850     419     349

Cuts at:   3634    4032    4201    4416    4642    6171    7764    9291 
   Size:        398     169     215     226    1529    1593    1527

  Fragments arranged by size: 

                1593    1529    1527     850     678     419     398     390
                 349     347     301     300     226     215     169
 
PasI CC'CwG_GG

Cuts at:      0    3735    6480    9291 
   Size:       3735    2745    2811

  Fragments arranged by size: 

                3735    2811    2745
 
PciI A'CATG_T

Cuts at:      0    1334    2012    4638    6167    7760    9291 
   Size:       1334     678    2626    1529    1593    1531

  Fragments arranged by size: 

                2626    1593    1531    1529    1334     678
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1611    1953    5046    8856    9291 
   Size:       1611     342    3093    3810     435

  Fragments arranged by size: 

                3810    3093    1611     435     342
 
PfoI T'CCnGG_A

Cuts at:      0    1610    2324    2633    2954    3932    4769    7015    8630 
   Size:       1610     714     309     321     978     837    2246    1615

Cuts at:   8630    8975    9134    9291 
   Size:        345     159     157

  Fragments arranged by size: 

                2246    1615    1610     978     837     714     345     321
                 309     159     157
 
PmlI CAC'GTG

Cuts at:      0    3165    5376    7583    8084    8273    9291 
   Size:       3165    2211    2207     501     189    1018

  Fragments arranged by size: 

                3165    2211    2207    1018     501     189
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     877     909    4508    4540    7715    7747    8804    8836 
   Size:        877      32    3599      32    3175      32    1057      32

Cuts at:   8836    8890    8922    9291 
   Size:         54      32     369

  Fragments arranged by size: 

                3599    3175    1057     877     369      54      32      32
                  32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     123     253     819    1434    2121    2196    2637    3716 
   Size:        123     130     566     615     687      75     441    1079

Cuts at:   3716    4205    4485    5958    8325    9099    9291 
   Size:        489     280    1473    2367     774     192

  Fragments arranged by size: 

                2367    1473    1079     774     687     615     566     489
                 441     280     192     130     123      75
 
PshAI GACnn'nnGTC

Cuts at:      0    3407    5084    5231    8621    9291 
   Size:       3407    1677     147    3390     670

  Fragments arranged by size: 

                3407    3390    1677     670     147
 
PsiI TTA'TAA

Cuts at:      0    4598    9291 
   Size:       4598    4693
 
PspOMI G'GGCC_C

Cuts at:      0    3141    5276    5292    6935    7117    8157    9291 
   Size:       3141    2135      16    1643     182    1040    1134

  Fragments arranged by size: 

                3141    2135    1643    1134    1040     182      16
 
PspXI vC'TCGA_Gb

Cuts at:      0    2404    7071    9291 
   Size:       2404    4667    2220

  Fragments arranged by size: 

                4667    2404    2220
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    4445    4477    9291 
   Size:       4445      32    4814

  Fragments arranged by size: 

                4814    4445      32
 
PstI C_TGCA'G

Cuts at:      0     437     914    1433    1700    2072    2165    2285    2360 
   Size:        437     477     519     267     372      93     120      75

Cuts at:   2360    2561    2738    2897    3073    6550    7068    7439    8534 
   Size:        201     177     159     176    3477     518     371    1095

Cuts at:   8534    8728    9095    9152    9269    9291 
   Size:        194     367      57     117      22

  Fragments arranged by size: 

                3477    1095     519     518     477     437     372     371
                 367     267     201     194     177     176     159     120
                 117      93      75      57      22
 
PvuII CAG'CTG

Cuts at:      0     786    1023    1275    1650    1701    1899    1953    2289 
   Size:        786     237     252     375      51     198      54     336

Cuts at:   2289    2361    2724    3603    4185    6324    6977    7804    9291 
   Size:         72     363     879     582    2139     653     827    1487

  Fragments arranged by size: 

                2139    1487     879     827     786     653     582     375
                 363     336     252     237     198      72      54      51
            
 
SacI G_AGCT'C

Cuts at:      0    2321    2351    3533    3899    5287    5726    7462    9291 
   Size:       2321      30    1182     366    1388     439    1736    1829

  Fragments arranged by size: 

                2321    1829    1736    1388    1182     439     366      30
            
 
SacII CC_GC'GG

Cuts at:      0      56    9291 
   Size:         56    9235
 
SanDI GG'GwC_CC

Cuts at:      0    4485    9291 
   Size:       4485    4806
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2326    2356    5398    9291 
   Size:       2326      30    3042    3893

  Fragments arranged by size: 

                3893    3042    2326      30
 
SbfI CC_TGCA'GG

Cuts at:      0     437    2165    2738    9095    9291 
   Size:        437    1728     573    6357     196

  Fragments arranged by size: 

                6357    1728     573     437     196
 
SexAI A'CCwGG_T

Cuts at:      0    8504    9291 
   Size:       8504     787
 
SfcI C'TryA_G

Cuts at:      0     433     910    1429    1696    2068    2161    2281    2356 
   Size:        433     477     519     267     372      93     120      75

Cuts at:   2356    2557    2734    2893    2923    3069    4462    6546    7064 
   Size:        201     177     159      30     146    1393    2084     518

Cuts at:   7064    7435    8193    8205    8385    8530    8724    9091    9148 
   Size:        371     758      12     180     145     194     367      57

Cuts at:   9148    9265    9291 
   Size:        117      26

  Fragments arranged by size: 

                2084    1393     758     519     518     477     433     372
                 371     367     267     201     194     180     177     159
                 146     145     120     117      93      75      57      30
                  26      12
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    7150    9291 
   Size:       7150    2141
 
SfoI GGC'GCC

Cuts at:      0    6987    7263    9291 
   Size:       6987     276    2028

  Fragments arranged by size: 

                6987    2028     276
 
SmaI CCC'GGG

Cuts at:      0    7116    7348    7508    9291 
   Size:       7116     232     160    1783

  Fragments arranged by size: 

                7116    1783     232     160
 
SmlI C'TyrA_G

Cuts at:      0    2404    3001    3019    3079    4711    6343    7071    7876 
   Size:       2404     597      18      60    1632    1632     728     805

Cuts at:   7876    9291 
   Size:       1415

  Fragments arranged by size: 

                2404    1632    1632    1415     805     728     597      60
                  18
 
SnaBI TAC'GTA

Cuts at:      0    3950    8949    9291 
   Size:       3950    4999     342

  Fragments arranged by size: 

                4999    3950     342
 
SpeI A'CTAG_T

Cuts at:      0    5928    9291 
   Size:       5928    3363
 
SphI G_CATG'C

Cuts at:      0    2866    3634    4416    9291 
   Size:       2866     768     782    4875

  Fragments arranged by size: 

                4875    2866     782     768
 
StuI AGG'CCT

Cuts at:      0     563    1082    2581    5126    5668    9291 
   Size:        563     519    1499    2545     542    3623

  Fragments arranged by size: 

                3623    2545    1499     563     542     519
 
StyI C'CwwG_G

Cuts at:      0     119     926    1130    1592    2541    4881    5615    5961 
   Size:        119     807     204     462     949    2340     734     346

Cuts at:   5961    7301    7719    8328    9068    9291 
   Size:       1340     418     609     740     223

  Fragments arranged by size: 

                2340    1340     949     807     740     734     609     462
                 418     346     223     204     119
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     697    2047    3538    4615    4662    5628    7633    8527 
   Size:        697    1350    1491    1077      47     966    2005     894

Cuts at:   8527    9291 
   Size:        764

  Fragments arranged by size: 

                2005    1491    1350    1077     966     894     764     697
                  47
 
TatI w'GTAC_w

Cuts at:      0    3098    3710    6170    9291 
   Size:       3098     612    2460    3121

  Fragments arranged by size: 

                3121    3098    2460     612
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1270    1302    1375    1407    2239    2271    2584    2616 
   Size:       1270      32      73      32     832      32     313      32

Cuts at:   2616    6388    6420    9064    9096    9291 
   Size:       3772      32    2644      32     195

  Fragments arranged by size: 

                3772    2644    1270     832     313     195      73      32
                  32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    8506    9291 
   Size:       8506     785
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     488     819    1221    3447    5169    5871    7552    8460 
   Size:        488     331     402    2226    1722     702    1681     908

Cuts at:   8460    8595    9291 
   Size:        135     696

  Fragments arranged by size: 

                2226    1722    1681     908     702     696     488     402
                 331     135
 
XhoI C'TCGA_G

Cuts at:      0    2404    3079    7071    9291 
   Size:       2404     675    3992    2220

  Fragments arranged by size: 

                3992    2404    2220     675
 
XmaI C'CCGG_G

Cuts at:      0    7114    7346    7506    9291 
   Size:       7114     232     160    1785

  Fragments arranged by size: 

                7114    1785     232     160
 
XmnI GAAnn'nnTTC

Cuts at:      0     872    1763    2443    3880    3983    6337    6616    9291 
   Size:        872     891     680    1437     103    2354     279    2675

  Fragments arranged by size: 

                2675    2354    1437     891     872     680     279     103
            
 
ZraI GAC'GTC

Cuts at:      0    2532    3246    4931    9291 
   Size:       2532     714    1685    4360

  Fragments arranged by size: 

                4360    2532    1685     714
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AvaI    AvrII    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    HaeII
    Hin4I   HincII  HindIII     KasI     KpnI     MfeI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NspI     PasI     PciI    PflMI     PfoI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI    PvuII
     SacI    SacII    SanDI     SapI     SbfI    SexAI     SfcI     SfiI
     SfoI     SmaI     SmlI    SnaBI     SpeI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AgeI     AscI     AseI    AsiSI     BaeI    BfrBI     BplI    BsiWI
    BspEI     ClaI    FspAI     HpaI     MluI     NdeI     NsiI     PacI
     PmeI     PvuI    RsrII     SalI     ScaI     SgfI    SgrAI     SrfI
     SspI     SwaI     XbaI
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