Restriction map for
pF1KE0969
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII AccII AccIII AclI AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspI AvaI AviII
AvrII BalI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BglI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmI BsoBI
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI Bsu36I CfoI
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoT14I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KspI MamI MluI MluNI MroI
MscI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 449 GGTACC G^GTACC
AccI 998 GTATAC GT^[AC][GT]AC
AflII 306 CTTAAG C^TTAAG
AlwNI 1500 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 449 GGTACC G^GTACC
AspHI 96 GAGCAC G[AT]GC[AT]^C
BamHI 544 GGATCC G^GATCC
BfrI 306 CTTAAG C^TTAAG
BlpI 147 GCTCAGC GC^T[ACGT]AGC
BmyI 96 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 147 GCTCAGC GC^T[ACGT]AGC
BsaJI 142 CCGTGG C^C[ACGT][ACGT]GG
BsiHKAI 96 GAGCAC G[AT]GC[AT]^C
BsmBI 721 CGTCTC CGTCTC(1/5)
BsmFI 1296 GTCCC GGGAC(10/14)
Bsp1286I 96 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 1496 TGTACA T^GTACA
BspHI 713 TCATGA T^CATGA
BsrFI 706 GCCGGT [GA]^CCGG[CT]
BsrGI 1496 TGTACA T^GTACA
Bst1107I 998 GTATAC GTA^TAC
BstXI 1285 CCAATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 998 GTATAC GTA^TAC
CelII 147 GCTCAGC GC^T[ACGT]AGC
Cfr10I 706 GCCGGT [GA]^CCGG[CT]
DsaI 142 CCGTGG C^C[GA][CT]GG
EcoRV 728 GATATC GAT^ATC
EcoT22I 1203 ATGCAT ATGCA^T
FauI 1551 GCGGG CCCGC(4/6)
HapII 707 CCGG C^CGG
HindIII 1081 AAGCTT A^AGCTT
HpaII 707 CCGG C^CGG
KpnI 449 GGTACC GGTAC^C
MaeII 1241 ACGT A^CGT
MfeI 154 CAATTG C^AATTG
MslI 856 CATTTAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 707 CCGG C^CGG
MunI 154 CAATTG C^AATTG
NsiI 1203 ATGCAT ATGCA^T
NspI 1201 ACATGC [GA]CATG^[CT]
PshAI 494 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 713 TCATGA T^CATGA
SapI 106 GAAGAGC GCTCTTC(1/4)
SfaNI 132 GCATC GCATC(5/9)
SmlI 306 CTTAAG C^T[CT][GA]AG
SspBI 1496 TGTACA T^GTACA
TfiI 529 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AcsI 1214 AAATTT [GA]^AATT[CT]
1413 AAATTT
ApoI 1214 AAATTT [GA]^AATT[CT]
1413 AAATTT
Asp700I 106 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1306 GAAGTTTTTC
AvaII 852 GGTCC G^G[AT]CC
1295 GGTCC
BanI 449 GGTACC G^G[CT][GA]CC
1470 GGCACC
BfaI 575 CTAG C^TAG
976 CTAG
BpmI 334 CTGGAG CTGGAG(16/14)
1141 CTGGAG
BsaI 368 GAGACC GGTCTC(1/5)
709 GGTCTC
BsgI 61 GTGCAG GTGCAG(16/14)
190 GTGCAG
BstF5I 24 CATCC GGATG(2/0)
1248 GGATG
FokI 24 CATCC GGATG(9/13)
1248 GGATG
MaeI 575 CTAG C^TAG
976 CTAG
PvuII 767 CAGCTG CAG^CTG
1500 CAGCTG
XmnI 106 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1306 GAAGTTTTTC
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BsiYI BslI BsmAI BsrI BssKI
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI HaeIII
HinfI HphI ItaI Ksp632I MaeIII
MboI MboII MflI MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII