Restriction map for pF1KE2320
 (Linear) (Six-base) MAPSORT of: KIEE2320.seq  Check: 1972  from: 1  to: 1758



KIEE2320.orf

 With 182 enzymes: SgfI * 

                        January 11, 2011 13:49  ..

AccI GT'mk_AC

Cuts at:      0     407    1758 
   Size:        407    1351
 
Acc65I G'GTAC_C

Cuts at:      0     579    1758 
   Size:        579    1179
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331    1042    1758 
   Size:        331     711     716

  Fragments arranged by size: 

                 716     711     331
 
AflIII A'CryG_T

Cuts at:      0     251    1758 
   Size:        251    1507
 
AleI CACnn'nnGTG

Cuts at:      0     433    1758 
   Size:        433    1325
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     357    1261    1758 
   Size:        357     904     497

  Fragments arranged by size: 

                 904     497     357
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1425    1758 
   Size:       1425     333
 
ApoI r'AATT_y

Cuts at:      0     472     610    1543    1758 
   Size:        472     138     933     215

  Fragments arranged by size: 

                 933     472     215     138
 
AvaI C'yCGr_G

Cuts at:      0    1616    1679    1758 
   Size:       1616      63      79

  Fragments arranged by size: 

                1616      79      63
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1327    1360    1758 
   Size:       1327      33     398

  Fragments arranged by size: 

                1327     398      33
 
BanI G'GyrC_C

Cuts at:      0     150     446     579    1237    1758 
   Size:        150     296     133     658     521

  Fragments arranged by size: 

                 658     521     296     150     133
 
BanII G_rGCy'C

Cuts at:      0      65    1606    1758 
   Size:         65    1541     152

  Fragments arranged by size: 

                1541     152      65
 
BbeI G_GCGC'C

Cuts at:      0     154    1758 
   Size:        154    1604
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     570    1758 
   Size:        570    1188
 
BbvCI CC'TCA_GC

Cuts at:      0    1001    1169    1758 
   Size:       1001     168     589

  Fragments arranged by size: 

                1001     589     168
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     880    1355    1758 
   Size:        880     475     403

  Fragments arranged by size: 

                 880     475     403
 
BglI GCCn_nnn'nGGC

Cuts at:      0     283    1758 
   Size:        283    1475
 
BlpI GC'TnA_GC

Cuts at:      0     419     463    1758 
   Size:        419      44    1295

  Fragments arranged by size: 

                1295     419      44
 
Bme1580I G_kGCm'C

Cuts at:      0     451    1240    1758 
   Size:        451     789     518

  Fragments arranged by size: 

                 789     518     451
 
BmgBI CAC'GTC

Cuts at:      0     252     426    1758 
   Size:        252     174    1332

  Fragments arranged by size: 

                1332     252     174
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     531    1758 
   Size:        531    1227
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1470    1758 
   Size:       1470     288
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1277    1590    1758 
   Size:       1277     313     168

  Fragments arranged by size: 

                1277     313     168
 
Bpu10I CC'TnA_GC

Cuts at:      0    1001    1169    1487    1758 
   Size:       1001     168     318     271

  Fragments arranged by size: 

                1001     318     271     168
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1606    1758 
   Size:       1606     152
 
BsaHI Gr'CG_yC

Cuts at:      0     151    1758 
   Size:        151    1607
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     357     387    1531    1561    1758 
   Size:        357      30    1144      30     197

  Fragments arranged by size: 

                1144     357     197      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      90     365    1539    1758 
   Size:         90     275    1174     219

  Fragments arranged by size: 

                1174     275     219      90
 
BseYI C'CCAG_C

Cuts at:      0     172    1758 
   Size:        172    1586
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1311    1758 
   Size:       1311     447
 
BsiHKAI G_wGCw'C

Cuts at:      0     274     425    1758 
   Size:        274     151    1333

  Fragments arranged by size: 

                1333     274     151
 
Bsp1286I G_dGCh'C

Cuts at:      0      65     274     425     451    1240    1606    1758 
   Size:         65     209     151      26     789     366     152

  Fragments arranged by size: 

                 789     366     209     152     151      65      26
 
BsrFI r'CCGG_y

Cuts at:      0    1529    1758 
   Size:       1529     229
 
BsrGI T'GTAC_A

Cuts at:      0     626    1758 
   Size:        626    1132
 
BstYI r'GATC_y

Cuts at:      0     549     746     898    1758 
   Size:        549     197     152     860

  Fragments arranged by size: 

                 860     549     197     152
 
Bsu36I CC'TnA_GG

Cuts at:      0     377    1758 
   Size:        377    1381
 
BtgI C'CryG_G

Cuts at:      0    1457    1758 
   Size:       1457     301
 
BtsI GCAGTG_nn'

Cuts at:      0    1323    1587    1758 
   Size:       1323     264     171

  Fragments arranged by size: 

                1323     264     171
 
EaeI y'GGCC_r

Cuts at:      0     284    1758 
   Size:        284    1474
 
EarI CTCTTCn'nnn_

Cuts at:      0     759    1758 
   Size:        759     999
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     793    1645    1758 
   Size:        793     852     113

  Fragments arranged by size: 

                 852     793     113
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331    1042    1277    1590    1758 
   Size:        331     711     235     313     168

  Fragments arranged by size: 

                 711     331     313     235     168
 
EcoRI G'AATT_C

Cuts at:      0     472     610    1543    1758 
   Size:        472     138     933     215

  Fragments arranged by size: 

                 933     472     215     138
 
HaeII r_GCGC'y

Cuts at:      0     154     846    1758 
   Size:        154     692     912

  Fragments arranged by size: 

                 912     692     154
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     178     210     357     374     389     406     480     512 
   Size:        178      32     147      17      15      17      74      32

Cuts at:    512    1490    1522    1637    1669    1758 
   Size:        978      32     115      32      89

  Fragments arranged by size: 

                 978     178     147     115      89      74      32      32
                  32      32      17      17      15
 
KasI G'GCGC_C

Cuts at:      0     150    1758 
   Size:        150    1608
 
KpnI G_GTAC'C

Cuts at:      0     583    1758 
   Size:        583    1175
 
MfeI C'AATT_G

Cuts at:      0    1010    1758 
   Size:       1010     748
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     242     636    1758 
   Size:        242     394    1122

  Fragments arranged by size: 

                1122     394     242
 
MslI CAynn'nnrTG

Cuts at:      0     433    1228    1243    1249    1758 
   Size:        433     795      15       6     509

  Fragments arranged by size: 

                 795     509     433      15       6
 
NarI GG'CG_CC

Cuts at:      0     151    1758 
   Size:        151    1607
 
NcoI C'CATG_G

Cuts at:      0    1457    1758 
   Size:       1457     301
 
NdeI CA'TA_TG

Cuts at:      0    1092    1758 
   Size:       1092     666
 
NspI r_CATG'y

Cuts at:      0     344    1248    1504    1758 
   Size:        344     904     256     254

  Fragments arranged by size: 

                 904     344     256     254
 
PasI CC'CwG_GG

Cuts at:      0    1419    1758 
   Size:       1419     339
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1255    1758 
   Size:       1255     503
 
PfoI T'CCnGG_A

Cuts at:      0    1520    1758 
   Size:       1520     238
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      85     117    1758 
   Size:         85      32    1641

  Fragments arranged by size: 

                1641      85      32
 
SfcI C'TryA_G

Cuts at:      0    1589    1758 
   Size:       1589     169
 
SfoI GGC'GCC

Cuts at:      0     152    1758 
   Size:        152    1606
 
SmaI CCC'GGG

Cuts at:      0    1618    1758 
   Size:       1618     140
 
StyI C'CwwG_G

Cuts at:      0    1457    1758 
   Size:       1457     301
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1255    1297    1758 
   Size:       1255      42     461

  Fragments arranged by size: 

                1255     461      42
 
TatI w'GTAC_w

Cuts at:      0      47     626     817    1758 
   Size:         47     579     191     941

  Fragments arranged by size: 

                 941     579     191      47
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     194     226     854     886    1531    1563    1758 
   Size:        194      32     628      32     645      32     195

  Fragments arranged by size: 

                 645     628     195     194      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1464    1758 
   Size:       1464     294
 
XmaI C'CCGG_G

Cuts at:      0    1616    1758 
   Size:       1616     142
 
XmnI GAAnn'nnTTC

Cuts at:      0    1696    1758 
   Size:       1696      62
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI   AflIII     AleI     AlfI    AlwNI     ApoI
     AvaI     BaeI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
     BsaI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BsrFI    BsrGI    BstYI   Bsu36I     BtgI     BtsI     EaeI     EarI
     EciI  Eco57MI    EcoRI    HaeII    Hin4I     KasI     KpnI     MfeI
     MmeI     MslI     NarI     NcoI     NdeI     NspI     PasI    PflMI
     PfoI     PpiI     SfcI     SfoI     SmaI     StyI    TaqII     TatI
     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII     AgeI     AhdI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII    BamHI     BcgI
     BclI    BfrBI    BglII     BmtI    BpuEI    BsaAI    BsaBI    BsaWI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI
    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     MluI     MscI   MspA1I     NaeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XhoI     ZraI
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