Restriction map for pF1KB9893
 (Linear) (Six-base) MAPSORT of: KIBB9893.seq  Check: 4326  from: 1  to: 3876



KIBB9893.orf

 With 182 enzymes: SgfI * 

                        January 5, 2011 13:33  ..

AccI GT'mk_AC

Cuts at:      0     446     800    2673    2889    3876 
   Size:        446     354    1873     216     987

  Fragments arranged by size: 

                1873     987     446     354     216
 
Acc65I G'GTAC_C

Cuts at:      0    2211    3876 
   Size:       2211    1665
 
AclI AA'CG_TT

Cuts at:      0    2811    3876 
   Size:       2811    1065
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1224    1369    1416    1514    1560    2173    2237    3637 
   Size:       1224     145      47      98      46     613      64    1400

Cuts at:   3637    3694    3841    3876 
   Size:         57     147      35

  Fragments arranged by size: 

                1400    1224     613     147     145      98      64      57
                  47      46      35
 
AflIII A'CryG_T

Cuts at:      0     257    3876 
   Size:        257    3619
 
AhdI GACnn_n'nnGTC

Cuts at:      0      22     673    2270    3876 
   Size:         22     651    1597    1606

  Fragments arranged by size: 

                1606    1597     651      22
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3853    3876 
   Size:       3853      23
 
AlwNI CAG_nnn'CTG

Cuts at:      0     683    1347    3832    3876 
   Size:        683     664    2485      44

  Fragments arranged by size: 

                2485     683     664      44
 
ApoI r'AATT_y

Cuts at:      0     300     457     750    1063    1092    1624    2686    2768 
   Size:        300     157     293     313      29     532    1062      82

Cuts at:   2768    2896    3876 
   Size:        128     980

  Fragments arranged by size: 

                1062     980     532     313     300     293     157     128
                  82      29
 
AvaI C'yCGr_G

Cuts at:      0    2933    3876 
   Size:       2933     943
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     118     151    3876 
   Size:        118      33    3725

  Fragments arranged by size: 

                3725     118      33
 
BamHI G'GATC_C

Cuts at:      0    3770    3876 
   Size:       3770     106
 
BanI G'GyrC_C

Cuts at:      0    2211    3277    3297    3876 
   Size:       2211    1066      20     579

  Fragments arranged by size: 

                2211    1066     579      20
 
BanII G_rGCy'C

Cuts at:      0     112    1982    2368    3201    3373    3876 
   Size:        112    1870     386     833     172     503

  Fragments arranged by size: 

                1870     833     503     386     172     112
 
BbvCI CC'TCA_GC

Cuts at:      0    2819    3056    3876 
   Size:       2819     237     820

  Fragments arranged by size: 

                2819     820     237
 
BclI T'GATC_A

Cuts at:      0    2309    3876 
   Size:       2309    1567
 
BfrBI ATG'CAT

Cuts at:      0    3523    3876 
   Size:       3523     353
 
Bme1580I G_kGCm'C

Cuts at:      0    3191    3876 
   Size:       3191     685
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1665    2093    2981    3876 
   Size:       1665     428     888     895

  Fragments arranged by size: 

                1665     895     888     428
 
BmtI G_CTAG'C

Cuts at:      0    2593    3876 
   Size:       2593    1283
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1662    3876 
   Size:       1662    2214
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     316     725     844    1985    2686    3876 
   Size:        208     108     409     119    1141     701    1190

  Fragments arranged by size: 

                1190    1141     701     409     208     119     108
 
Bpu10I CC'TnA_GC

Cuts at:      0     714    2175    2483    2819    3056    3876 
   Size:        714    1461     308     336     237     820

  Fragments arranged by size: 

                1461     820     714     336     308     237
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1294    2076    3876 
   Size:       1294     782    1800

  Fragments arranged by size: 

                1800    1294     782
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2930    3479    3876 
   Size:       2930     549     397

  Fragments arranged by size: 

                2930     549     397
 
BsaAI yAC'GTr

Cuts at:      0     258    3876 
   Size:        258    3618
 
BsaBI GATnn'nnATC

Cuts at:      0    1829    3876 
   Size:       1829    2047
 
BsaWI w'CCGG_w

Cuts at:      0    2342    3860    3876 
   Size:       2342    1518      16

  Fragments arranged by size: 

                2342    1518      16
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     428     458     758     788    2213    2243    3170    3200 
   Size:        428      30     300      30    1425      30     927      30

Cuts at:   3200    3876 
   Size:        676

  Fragments arranged by size: 

                1425     927     676     428     300      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1687    3073    3392    3466    3876 
   Size:       1687    1386     319      74     410

  Fragments arranged by size: 

                1687    1386     410     319      74
 
BseYI C'CCAG_C

Cuts at:      0      64     590    2349    3876 
   Size:         64     526    1759    1527

  Fragments arranged by size: 

                1759    1527     526      64
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     107     659    1845    2817    3876 
   Size:        107     552    1186     972    1059

  Fragments arranged by size: 

                1186    1059     972     552     107
 
BsiHKAI G_wGCw'C

Cuts at:      0     112     209     830    1741    2432    3706    3876 
   Size:        112      97     621     911     691    1274     170

  Fragments arranged by size: 

                1274     911     691     621     170     112      97
 
BsmI GAATG_Cn'

Cuts at:      0     859    3876 
   Size:        859    3017
 
Bsp1286I G_dGCh'C

Cuts at:      0     112     209     830    1741    1982    2368    2432    3191 
   Size:        112      97     621     911     241     386      64     759

Cuts at:   3191    3201    3373    3706    3876 
   Size:         10     172     333     170

  Fragments arranged by size: 

                 911     759     621     386     333     241     172     170
                 112      97      64      10
 
BspHI T'CATG_A

Cuts at:      0    1890    3876 
   Size:       1890    1986
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     296    3734    3876 
   Size:        296    3438     142

  Fragments arranged by size: 

                3438     296     142
 
BsrDI GCAATG_nn'

Cuts at:      0     609    3876 
   Size:        609    3267
 
BsrFI r'CCGG_y

Cuts at:      0       6    3876 
   Size:          6    3870
 
BsrGI T'GTAC_A

Cuts at:      0    1729    3876 
   Size:       1729    2147
 
BssSI C'ACGA_G

Cuts at:      0      40    2425    3374    3876 
   Size:         40    2385     949     502

  Fragments arranged by size: 

                2385     949     502      40
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3238    3876 
   Size:       3238     638
 
BstEII G'GTnAC_C

Cuts at:      0    1260    3876 
   Size:       1260    2616
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1656    2379    2601    2654    3597    3876 
   Size:       1656     723     222      53     943     279

  Fragments arranged by size: 

                1656     943     723     279     222      53
 
BstYI r'GATC_y

Cuts at:      0    1589    3770    3876 
   Size:       1589    2181     106

  Fragments arranged by size: 

                2181    1589     106
 
BstZ17I GTA'TAC

Cuts at:      0    2674    3876 
   Size:       2674    1202
 
Bsu36I CC'TnA_GG

Cuts at:      0     722    3074    3876 
   Size:        722    2352     802

  Fragments arranged by size: 

                2352     802     722
 
BtgI C'CryG_G

Cuts at:      0     508    1707    1909    2400    3876 
   Size:        508    1199     202     491    1476

  Fragments arranged by size: 

                1476    1199     508     491     202
 
ClaI AT'CG_AT

Cuts at:      0     339    3876 
   Size:        339    3537
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     744     779    1748    1783    3336    3371    3876 
   Size:        744      35     969      35    1553      35     505

  Fragments arranged by size: 

                1553     969     744     505      35      35      35
 
DraI TTT'AAA

Cuts at:      0     362    1749    3876 
   Size:        362    1387    2127

  Fragments arranged by size: 

                2127    1387     362
 
DraIII CAC_nnn'GTG

Cuts at:      0    3238    3876 
   Size:       3238     638
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     531    3876 
   Size:        531    3345
 
EaeI y'GGCC_r

Cuts at:      0     582    1219    3876 
   Size:        582     637    2657

  Fragments arranged by size: 

                2657     637     582
 
EarI CTCTTCn'nnn_

Cuts at:      0    1269    1435    1987    2026    2149    3023    3876 
   Size:       1269     166     552      39     123     874     853

  Fragments arranged by size: 

                1269     874     853     552     166     123      39
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3783    3876 
   Size:       3783      93
 
EcoICRI GAG'CTC

Cuts at:      0     110    3876 
   Size:        110    3766
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     316     725     844    1224    1369    1416    1514 
   Size:        208     108     409     119     380     145      47      98

Cuts at:   1514    1560    1985    2173    2237    2686    3637    3694    3841 
   Size:         46     425     188      64     449     951      57     147

Cuts at:   3841    3876 
   Size:         35

  Fragments arranged by size: 

                 951     449     425     409     380     208     188     147
                 145     119     108      98      64      57      47      46
                  35
 
EcoO109I rG'GnC_Cy

Cuts at:      0     216     595     718    1125    1540    1717    3876 
   Size:        216     379     123     407     415     177    2159

  Fragments arranged by size: 

                2159     415     407     379     216     177     123
 
EcoRI G'AATT_C

Cuts at:      0    2768    3876 
   Size:       2768    1108
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2143    3876 
   Size:       2143    1733
 
FspI TGC'GCA

Cuts at:      0     148    3876 
   Size:        148    3728
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       8      40      61      93     659     691    1017    1049 
   Size:          8      32      21      32     566      32     326      32

Cuts at:   1049    1574    1606    2256    2288    3876 
   Size:        525      32     650      32    1588

  Fragments arranged by size: 

                1588     650     566     525     326      32      32      32
                  32      32      32      21       8
 
HincII GTy'rAC

Cuts at:      0     536    3876 
   Size:        536    3340
 
HindIII A'AGCT_T

Cuts at:      0     995    2980    3876 
   Size:        995    1985     896

  Fragments arranged by size: 

                1985     995     896
 
KpnI G_GTAC'C

Cuts at:      0    2215    3876 
   Size:       2215    1661
 
MfeI C'AATT_G

Cuts at:      0    1139    3876 
   Size:       1139    2737
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     542     832    2393    2797    2848    3876 
   Size:        542     290    1561     404      51    1028

  Fragments arranged by size: 

                1561    1028     542     404     290      51
 
MscI TGG'CCA

Cuts at:      0    1221    3876 
   Size:       1221    2655
 
MslI CAynn'nnrTG

Cuts at:      0    1654    2699    3876 
   Size:       1654    1045    1177

  Fragments arranged by size: 

                1654    1177    1045
 
MspA1I CmG'CkG

Cuts at:      0    1963    2379    3876 
   Size:       1963     416    1497

  Fragments arranged by size: 

                1963    1497     416
 
NaeI GCC'GGC

Cuts at:      0       8    3876 
   Size:          8    3868
 
NcoI C'CATG_G

Cuts at:      0     508    1707    1909    2400    3876 
   Size:        508    1199     202     491    1476

  Fragments arranged by size: 

                1476    1199     508     491     202
 
NdeI CA'TA_TG

Cuts at:      0     852    3876 
   Size:        852    3024
 
NgoMIV G'CCGG_C

Cuts at:      0       6    3876 
   Size:          6    3870
 
NheI G'CTAG_C

Cuts at:      0    2589    3876 
   Size:       2589    1287
 
NsiI A_TGCA'T

Cuts at:      0    3525    3876 
   Size:       3525     351
 
NspI r_CATG'y

Cuts at:      0     773    1706    1942    3876 
   Size:        773     933     236    1934

  Fragments arranged by size: 

                1934     933     773     236
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     485    1418    3876 
   Size:        485     933    2458

  Fragments arranged by size: 

                2458     933     485
 
PfoI T'CCnGG_A

Cuts at:      0    1460    3876 
   Size:       1460    2416
 
PpuMI rG'GwC_Cy

Cuts at:      0     216     595     718    1125    1540    1717    3876 
   Size:        216     379     123     407     415     177    2159

  Fragments arranged by size: 

                2159     415     407     379     216     177     123
 
PshAI GACnn'nnGTC

Cuts at:      0    1556    3876 
   Size:       1556    2320
 
PsiI TTA'TAA

Cuts at:      0    2198    3755    3876 
   Size:       2198    1557     121

  Fragments arranged by size: 

                2198    1557     121
 
PstI C_TGCA'G

Cuts at:      0    3731    3876 
   Size:       3731     145
 
PvuII CAG'CTG

Cuts at:      0    1963    2379    3876 
   Size:       1963     416    1497

  Fragments arranged by size: 

                1963    1497     416
 
SacI G_AGCT'C

Cuts at:      0     112    3876 
   Size:        112    3764
 
SanDI GG'GwC_CC

Cuts at:      0     595    1125    3876 
   Size:        595     530    2751

  Fragments arranged by size: 

                2751     595     530
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1987    2026    3876 
   Size:       1987      39    1850

  Fragments arranged by size: 

                1987    1850      39
 
SbfI CC_TGCA'GG

Cuts at:      0    3731    3876 
   Size:       3731     145
 
ScaI AGT'ACT

Cuts at:      0     154    1280    3064    3876 
   Size:        154    1126    1784     812

  Fragments arranged by size: 

                1784    1126     812     154
 
SexAI A'CCwGG_T

Cuts at:      0    1256    3876 
   Size:       1256    2620
 
SfcI C'TryA_G

Cuts at:      0     366    1284    1333    3192    3727    3876 
   Size:        366     918      49    1859     535     149

  Fragments arranged by size: 

                1859     918     535     366     149      49
 
SmlI C'TyrA_G

Cuts at:      0    1309    2055    2933    3876 
   Size:       1309     746     878     943

  Fragments arranged by size: 

                1309     943     878     746
 
SpeI A'CTAG_T

Cuts at:      0    1885    3876 
   Size:       1885    1991
 
SspI AAT'ATT

Cuts at:      0     356    3748    3876 
   Size:        356    3392     128

  Fragments arranged by size: 

                3392     356     128
 
StyI C'CwwG_G

Cuts at:      0     347     508    1707    1866    1909    2169    2276    2400 
   Size:        347     161    1199     159      43     260     107     124

Cuts at:   2400    3539    3596    3876 
   Size:       1139      57     280

  Fragments arranged by size: 

                1199    1139     347     280     260     161     159     124
                 107      57      43
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      18    1859    1879    2127    2341    2707    3340    3552 
   Size:         18    1841      20     248     214     366     633     212

Cuts at:   3552    3731    3876 
   Size:        179     145

  Fragments arranged by size: 

                1841     633     366     248     214     212     179     145
                  20      18
 
TatI w'GTAC_w

Cuts at:      0     152     577     845    1278    1486    1729    2621    3062 
   Size:        152     425     268     433     208     243     892     441

Cuts at:   3062    3734    3876 
   Size:        672     142

  Fragments arranged by size: 

                 892     672     441     433     425     268     243     208
                 152     142
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     621     653    2213    2245    2324    2356    3170    3202 
   Size:        621      32    1560      32      79      32     814      32

Cuts at:   3202    3290    3322    3508    3540    3876 
   Size:         88      32     186      32     336

  Fragments arranged by size: 

                1560     814     621     336     186      88      79      32
                  32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1715    3876 
   Size:       1715    2161
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     819    2283    3876 
   Size:        819    1464    1593

  Fragments arranged by size: 

                1593    1464     819
 
XhoI C'TCGA_G

Cuts at:      0    2933    3876 
   Size:       2933     943
 
XmnI GAAnn'nnTTC

Cuts at:      0     457    3876 
   Size:        457    3419
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AhdI     AlfI    AlwNI
     ApoI     AvaI     BaeI    BamHI     BanI    BanII    BbvCI     BclI
    BfrBI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    Hin4I   HincII
  HindIII     KpnI     MfeI     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI     NdeI   NgoMIV     NheI     NsiI     NspI    PflMI     PfoI
    PpuMI    PshAI     PsiI     PstI    PvuII     SacI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SmlI     SpeI     SspI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AleI     AloI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BbeI     BbsI     BcgI
    BciVI     BglI    BglII     BlpI    BmgBI    BsaHI    BsiEI    BsiWI
    BsmBI    BspEI    BsrBI   BssHII    BstBI    BtgZI     BtsI     EagI
    EcoNI    EcoRV     FseI    FspAI    HaeII     HpaI     KasI     MluI
     NarI     NotI     NruI     PacI     PasI     PciI     PmeI     PmlI
     PpiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI
     StuI     SwaI     XbaI     XmaI     ZraI
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