(Circular) (Six-base) MAPSORT of: pF1KE2310.seq Check: 5293 from: 1 to: 7037 pF1KE2310 7037 bp With 182 enzymes: SgfI * January 5, 2011 13:33 .. AccI GT'mk_AC Cuts at: 554 908 2781 2997 4075 554 Size: 354 1873 216 1078 3516 Fragments arranged by size: 3516 1873 1078 354 216 Acc65I G'GTAC_C Cuts at: 2319 4053 2319 Size: 1734 5303 AclI AA'CG_TT Cuts at: 2919 6877 2919 Size: 3958 3079 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1332 1477 1524 1622 1668 2281 2345 3793 3850 Size: 145 47 98 46 613 64 1448 57 Cuts at: 3850 3997 4862 5294 6041 6309 1332 Size: 147 865 432 747 268 2060 Fragments arranged by size: 2060 1448 865 747 613 432 268 147 145 98 64 57 47 46 AflIII A'CryG_T Cuts at: 365 4389 5493 6470 365 Size: 4024 1104 977 932 Fragments arranged by size: 4024 1104 977 932 AgeI A'CCGG_T Cuts at: 5425 5425 Size: 7037 AhdI GACnn_n'nnGTC Cuts at: 130 781 2378 130 Size: 651 1597 4789 Fragments arranged by size: 4789 1597 651 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 4009 4009 Size: 7037 AlwNI CAG_nnn'CTG Cuts at: 791 1455 3988 4342 5909 791 Size: 664 2533 354 1567 1919 Fragments arranged by size: 2533 1919 1567 664 354 ApaLI G'TGCA_C Cuts at: 5807 5807 Size: 7037 ApoI r'AATT_y Cuts at: 408 565 858 1171 1200 1732 2794 2876 3004 Size: 157 293 313 29 532 1062 82 128 Cuts at: 3004 4041 6390 6646 408 Size: 1037 2349 256 799 Fragments arranged by size: 2349 1062 1037 799 532 313 293 256 157 128 82 29 AseI AT'TA_AT Cuts at: 20 20 Size: 7037 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 7037 AvaI C'yCGr_G Cuts at: 3041 4057 3041 Size: 1016 6021 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 226 259 226 Size: 33 7004 BamHI G'GATC_C Cuts at: 3926 4062 3926 Size: 136 6901 BanI G'GyrC_C Cuts at: 2319 3433 3453 4053 4467 4715 4750 2319 Size: 1114 20 600 414 248 35 4606 Fragments arranged by size: 4606 1114 600 414 248 35 20 BanII G_rGCy'C Cuts at: 220 2090 2476 3357 3529 4051 5081 220 Size: 1870 386 881 172 522 1030 2176 Fragments arranged by size: 2176 1870 1030 881 522 386 172 BbeI G_GCGC'C Cuts at: 4471 4719 4471 Size: 248 6789 BbvCI CC'TCA_GC Cuts at: 2927 3164 2927 Size: 237 6800 BciVI GTATCCnnnnn_n' Cuts at: 4935 5696 7037 4935 Size: 761 1341 4935 Fragments arranged by size: 4935 1341 761 BclI T'GATC_A Cuts at: 2417 4557 2417 Size: 2140 4897 BfrBI ATG'CAT Cuts at: 3679 3679 Size: 7037 BglII A'GATC_T Cuts at: 4552 6589 4552 Size: 2037 5000 BlpI GC'TnA_GC Cuts at: 4149 4149 Size: 7037 Bme1580I G_kGCm'C Cuts at: 3347 4662 4755 5811 3347 Size: 1315 93 1056 4573 Fragments arranged by size: 4573 1315 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1773 2201 3089 4249 4330 4665 6809 1773 Size: 428 888 1160 81 335 2144 2001 Fragments arranged by size: 2144 2001 1160 888 428 335 81 BmtI G_CTAG'C Cuts at: 2701 2701 Size: 7037 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1770 5404 1770 Size: 3634 3403 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 316 424 833 952 2093 2794 316 Size: 108 409 119 1141 701 4559 Fragments arranged by size: 4559 1141 701 409 119 108 Bpu10I CC'TnA_GC Cuts at: 822 2283 2591 2927 3164 822 Size: 1461 308 336 237 4695 Fragments arranged by size: 4695 1461 336 308 237 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1402 2184 4213 5092 5584 5882 1402 Size: 782 2029 879 492 298 2557 Fragments arranged by size: 2557 2029 879 782 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3038 3635 6741 3038 Size: 597 3106 3334 Fragments arranged by size: 3334 3106 597 BsaAI yAC'GTr Cuts at: 366 4390 366 Size: 4024 3013 BsaBI GATnn'nnATC Cuts at: 6 1937 4551 6588 6 Size: 1931 2614 2037 455 Fragments arranged by size: 2614 2037 1931 455 BsaHI Gr'CG_yC Cuts at: 4468 4716 5418 6914 4468 Size: 248 702 1496 4591 Fragments arranged by size: 4591 1496 702 248 BsaWI w'CCGG_w Cuts at: 2450 4016 4226 4747 5425 5699 5846 6257 2450 Size: 1566 210 521 678 274 147 411 3230 Fragments arranged by size: 3230 1566 678 521 411 274 210 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 536 566 866 896 2321 2351 3326 3356 536 Size: 30 300 30 1425 30 975 30 4217 Fragments arranged by size: 4217 1425 975 300 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1795 3181 3254 3548 3622 1795 Size: 1386 73 294 74 5210 Fragments arranged by size: 5210 1386 294 74 73 BseYI C'CCAG_C Cuts at: 172 698 2457 4462 5220 5797 6293 172 Size: 526 1759 2005 758 577 496 916 Fragments arranged by size: 2005 1759 916 758 577 526 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 215 767 1953 2925 215 Size: 552 1186 972 4327 Fragments arranged by size: 4327 1186 972 552 BsiEI CG_ry'CG Cuts at: 104 4579 4625 5833 104 Size: 4475 46 1208 1308 Fragments arranged by size: 4475 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 220 317 938 1849 2540 3862 4051 4829 5019 Size: 97 621 911 691 1322 189 778 190 Cuts at: 5019 5811 220 Size: 792 1446 Fragments arranged by size: 1446 1322 911 792 778 691 621 190 189 97 BsmI GAATG_Cn' Cuts at: 967 967 Size: 7037 Bsp1286I G_dGCh'C Cuts at: 220 317 938 1849 2090 2476 2540 3347 3357 Size: 97 621 911 241 386 64 807 10 Cuts at: 3357 3529 3862 4051 4662 4755 4829 5019 5081 Size: 172 333 189 611 93 74 190 62 Cuts at: 5081 5811 220 Size: 730 1446 Fragments arranged by size: 1446 911 807 730 621 611 386 333 241 190 189 172 97 93 74 64 62 10 BspEI T'CCGG_A Cuts at: 6257 6257 Size: 7037 BspHI T'CATG_A Cuts at: 1998 6213 1998 Size: 4215 2822 BspMI ACCTGCnnnn'nnnn_ Cuts at: 404 3890 4087 4301 4603 4984 404 Size: 3486 197 214 302 381 2457 Fragments arranged by size: 3486 2457 381 302 214 197 BsrBI CCG'CTC Cuts at: 50 5329 5383 6867 7032 50 Size: 5279 54 1484 165 55 Fragments arranged by size: 5279 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 717 4949 717 Size: 4232 2805 BsrFI r'CCGG_y Cuts at: 114 5035 5216 5425 114 Size: 4921 181 209 1726 Fragments arranged by size: 4921 1726 209 181 BsrGI T'GTAC_A Cuts at: 1837 1837 Size: 7037 BssSI C'ACGA_G Cuts at: 148 2533 3530 5308 5666 148 Size: 2385 997 1778 358 1519 Fragments arranged by size: 2385 1778 1519 997 358 BstAPI GCAn_nnn'nTGC Cuts at: 3394 4414 3394 Size: 1020 6017 BstBI TT'CG_AA Cuts at: 5398 5398 Size: 7037 BstEII G'GTnAC_C Cuts at: 1368 1368 Size: 7037 BstXI CCAn_nnnn'nTGG Cuts at: 1764 2487 2709 2762 3753 1764 Size: 723 222 53 991 5048 Fragments arranged by size: 5048 991 723 222 53 BstYI r'GATC_y Cuts at: 1 1697 3926 4062 4529 4552 4887 5133 6145 Size: 1696 2229 136 467 23 335 246 1012 Cuts at: 6145 6231 6243 6589 1 Size: 86 12 346 449 Fragments arranged by size: 2229 1696 1012 467 449 346 335 246 136 86 23 12 BstZ17I GTA'TAC Cuts at: 2782 2782 Size: 7037 Bsu36I CC'TnA_GG Cuts at: 830 3182 830 Size: 2352 4685 BtgI C'CryG_G Cuts at: 616 1815 2017 2508 616 Size: 1199 202 491 5145 Fragments arranged by size: 5145 1199 491 202 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 4987 5168 5331 4987 Size: 181 163 6693 Fragments arranged by size: 6693 181 163 BtsI GCAGTG_nn' Cuts at: 4280 4280 Size: 7037 ClaI AT'CG_AT Cuts at: 447 447 Size: 7037 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 852 887 1856 1891 3492 3527 852 Size: 35 969 35 1601 35 4362 Fragments arranged by size: 4362 1601 969 35 35 35 DraI TTT'AAA Cuts at: 470 1857 4036 470 Size: 1387 2179 3471 Fragments arranged by size: 3471 2179 1387 DraIII CAC_nnn'GTG Cuts at: 3394 3394 Size: 7037 DrdI GACnn_nn'nnGTC Cuts at: 639 4743 5601 639 Size: 4104 858 2075 Fragments arranged by size: 4104 2075 858 EaeI y'GGCC_r Cuts at: 690 1327 4622 4796 5187 5214 6559 690 Size: 637 3295 174 391 27 1345 1168 Fragments arranged by size: 3295 1345 1168 637 391 174 27 EagI C'GGCC_G Cuts at: 4622 4622 Size: 7037 EarI CTCTTCn'nnn_ Cuts at: 1377 1543 2095 2134 2257 3131 5060 5270 1377 Size: 166 552 39 123 874 1929 210 3144 Fragments arranged by size: 3144 1929 874 552 210 166 123 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3939 5245 5555 5701 6847 3939 Size: 1306 310 146 1146 4129 Fragments arranged by size: 4129 1306 1146 310 146 EcoICRI GAG'CTC Cuts at: 218 4049 218 Size: 3831 3206 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 316 424 833 952 1332 1477 1524 1622 1668 Size: 108 409 119 380 145 47 98 46 Cuts at: 1668 2093 2281 2345 2794 3793 3850 3997 4862 Size: 425 188 64 449 999 57 147 865 Cuts at: 4862 5294 6041 6309 316 Size: 432 747 268 1044 Fragments arranged by size: 1044 999 865 747 449 432 425 409 380 268 188 147 145 119 108 98 64 57 47 46 EcoO109I rG'GnC_Cy Cuts at: 324 703 826 1233 1648 1825 4176 324 Size: 379 123 407 415 177 2351 3185 Fragments arranged by size: 3185 2351 415 407 379 177 123 EcoRI G'AATT_C Cuts at: 2876 4041 2876 Size: 1165 5872 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2251 5056 2251 Size: 2805 4232 FspI TGC'GCA Cuts at: 256 4818 256 Size: 4562 2475 HaeII r_GCGC'y Cuts at: 4471 4719 5741 6720 4471 Size: 248 1022 979 4788 Fragments arranged by size: 4788 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 116 148 169 201 767 799 1125 1157 1682 Size: 32 21 32 566 32 326 32 525 Cuts at: 1682 1714 2364 2396 4879 4909 4911 4941 116 Size: 32 650 32 2483 30 2 30 2212 Fragments arranged by size: 2483 2212 650 566 525 326 32 32 32 32 32 32 30 30 21 2 HincII GTy'rAC Cuts at: 644 4076 644 Size: 3432 3605 HindIII A'AGCT_T Cuts at: 1103 3088 1103 Size: 1985 5052 KasI G'GCGC_C Cuts at: 4467 4715 4467 Size: 248 6789 KpnI G_GTAC'C Cuts at: 2323 4057 2323 Size: 1734 5303 MfeI C'AATT_G Cuts at: 1247 1247 Size: 7037 MluI A'CGCG_T Cuts at: 6470 6470 Size: 7037 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 650 940 2501 2905 2956 5708 5892 650 Size: 290 1561 404 51 2752 184 1795 Fragments arranged by size: 2752 1795 1561 404 290 184 51 MscI TGG'CCA Cuts at: 1329 4798 1329 Size: 3469 3568 MslI CAynn'nnrTG Cuts at: 1762 2807 3280 5026 5153 1762 Size: 1045 473 1746 127 3646 Fragments arranged by size: 3646 1746 1045 473 127 MspA1I CmG'CkG Cuts at: 2071 2487 4148 4462 4822 5835 6080 2071 Size: 416 1661 314 360 1013 245 3028 Fragments arranged by size: 3028 1661 1013 416 360 314 245 NaeI GCC'GGC Cuts at: 116 5218 116 Size: 5102 1935 NarI GG'CG_CC Cuts at: 4468 4716 4468 Size: 248 6789 NcoI C'CATG_G Cuts at: 616 1815 2017 2508 616 Size: 1199 202 491 5145 Fragments arranged by size: 5145 1199 491 202 NdeI CA'TA_TG Cuts at: 960 960 Size: 7037 NgoMIV G'CCGG_C Cuts at: 114 5216 114 Size: 5102 1935 NheI G'CTAG_C Cuts at: 2697 2697 Size: 7037 NsiI A_TGCA'T Cuts at: 3681 3681 Size: 7037 NspI r_CATG'y Cuts at: 881 1814 2050 4090 4590 5497 881 Size: 933 236 2040 500 907 2421 Fragments arranged by size: 2421 2040 933 907 500 236 PciI A'CATG_T Cuts at: 5493 5493 Size: 7037 PflMI CCAn_nnn'nTGG Cuts at: 593 1526 593 Size: 933 6104 PfoI T'CCnGG_A Cuts at: 1568 1568 Size: 7037 PmeI GTTT'AAAC Cuts at: 4036 4036 Size: 7037 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6176 6208 6176 Size: 32 7005 PpuMI rG'GwC_Cy Cuts at: 324 703 826 1233 1648 1825 324 Size: 379 123 407 415 177 5536 Fragments arranged by size: 5536 415 407 379 177 123 PshAI GACnn'nnGTC Cuts at: 1664 1664 Size: 7037 PsiI TTA'TAA Cuts at: 2306 3911 2306 Size: 1605 5432 PstI C_TGCA'G Cuts at: 3887 4084 4769 3887 Size: 197 685 6155 Fragments arranged by size: 6155 685 197 PvuI CG_AT'CG Cuts at: 104 104 Size: 7037 PvuII CAG'CTG Cuts at: 2071 2487 4462 4822 2071 Size: 416 1975 360 4286 Fragments arranged by size: 4286 1975 416 360 RsrII CG'GwC_CG Cuts at: 5232 5232 Size: 7037 SacI G_AGCT'C Cuts at: 220 4051 220 Size: 3831 3206 SalI G'TCGA_C Cuts at: 4074 4074 Size: 7037 SanDI GG'GwC_CC Cuts at: 703 1233 703 Size: 530 6507 SapI GCTCTTCn'nnn_ Cuts at: 2095 2134 5060 5270 2095 Size: 39 2926 210 3862 Fragments arranged by size: 3862 2926 210 39 SbfI CC_TGCA'GG Cuts at: 3887 4084 3887 Size: 197 6840 ScaI AGT'ACT Cuts at: 262 1388 3172 6554 262 Size: 1126 1784 3382 745 Fragments arranged by size: 3382 1784 1126 745 SexAI A'CCwGG_T Cuts at: 1364 1364 Size: 7037 SfcI C'TryA_G Cuts at: 33 474 1392 1441 3348 3883 4080 4765 5758 Size: 441 918 49 1907 535 197 685 993 Cuts at: 5758 5949 33 Size: 191 1121 Fragments arranged by size: 1907 1121 993 918 685 535 441 197 191 49 SfoI GGC'GCC Cuts at: 4469 4717 4469 Size: 248 6789 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 7037 SmaI CCC'GGG Cuts at: 4059 4059 Size: 7037 SmlI C'TyrA_G Cuts at: 1417 2163 3041 4192 5107 5599 5861 1417 Size: 746 878 1151 915 492 262 2593 Fragments arranged by size: 2593 1151 915 878 746 492 262 SpeI A'CTAG_T Cuts at: 67 1993 67 Size: 1926 5111 SphI G_CATG'C Cuts at: 4090 4590 4090 Size: 500 6537 SspI AAT'ATT Cuts at: 464 3904 464 Size: 3440 3597 StyI C'CwwG_G Cuts at: 455 616 1815 1974 2017 2277 2384 2508 3695 Size: 161 1199 159 43 260 107 124 1187 Cuts at: 3695 3752 4171 4525 455 Size: 57 419 354 2967 Fragments arranged by size: 2967 1199 1187 419 354 260 161 159 124 107 57 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 126 1967 1987 2235 2449 2815 3496 3708 3887 Size: 1841 20 248 214 366 681 212 179 Cuts at: 3887 4620 4753 5212 5421 126 Size: 733 133 459 209 1742 Fragments arranged by size: 1841 1742 733 681 459 366 248 214 212 209 179 133 20 TatI w'GTAC_w Cuts at: 260 685 953 1386 1594 1837 2729 3170 3890 Size: 425 268 433 208 243 892 441 720 Cuts at: 3890 6552 260 Size: 2662 745 Fragments arranged by size: 2662 892 745 720 441 433 425 268 243 208 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 729 761 2321 2353 2432 2464 3326 3358 3446 Size: 32 1560 32 79 32 862 32 88 Cuts at: 3446 3478 3664 3696 6554 6586 729 Size: 32 186 32 2858 32 1180 Fragments arranged by size: 2858 1560 1180 862 186 88 79 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1823 4834 1823 Size: 3011 4026 XbaI T'CTAG_A Cuts at: 4068 4068 Size: 7037 XcmI CCAnnnn_n'nnnnTGG Cuts at: 927 2391 4532 927 Size: 1464 2141 3432 Fragments arranged by size: 3432 2141 1464 XhoI C'TCGA_G Cuts at: 3041 3041 Size: 7037 XmaI C'CCGG_G Cuts at: 4057 4057 Size: 7037 XmnI GAAnn'nnTTC Cuts at: 565 565 Size: 7037 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AleI AloI ApaI AscI AvrII BbsI BcgI BglI BmgBI BsiWI BsmBI BssHII EcoNI EcoRV FseI FspAI HpaI NotI NruI PacI PasI PmlI PspOMI PspXI PsrI SacII SfiI SgrAI SnaBI SrfI StuI SwaI ZraI