Restriction map for pF1KE2394
##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

Acc65I      AccIII      AclI        AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AviII       
AvrII       BalI        BamHI       BbvCI       BcgI        
BglI        BglII       BlnI        BseAI       BsiEI       
BsiWI       Bsp1407I    BspDI       BspEI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoRI       EcoT22I     
FseI        FspI        KpnI        KspI        MluI        
MluNI       MroI        MscI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PinAI       PmeI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RsrII       SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI         1249  GTCTAC               GT^[AC][GT]AC
AflII        2136  CTTAAG               C^TTAAG
AflIII       1929  ACATGT               A^C[GA][CT]GT
ApaI          854  GGGCCC               GGGCC^C
AspI         1827  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          647  CTCGAG               C^[CT]CG[GA]G
BanI           28  GGCGCC               G^G[CT][GA]CC
BbeI           28  GGCGCC               GGCGC^C
BfaI         1996  CTAG                 C^TAG
BfrI         2136  CTTAAG               C^TTAAG
BsaI          628  GAGACC               GGTCTC(1/5)
BsaWI        1946  TCCGGT               [AT]^CCGG[AT]
BsgI          788  GTGCAG               GTGCAG(16/14)
BsoBI         647  CTCGAG               C^[CT]CG[GA]G
BspLU11I     1929  ACATGT               A^CATGT
BsrBI         456  GAGCGG               CCGCTC(-3/-3)
BsrFI          31  GCCGGC               [GA]^CCGG[CT]
BstAPI        417  GCAGGTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1186  GGTGACC              G^GT[ACGT]ACC
BstPI        1186  GGTGACC              G^GT[ACGT]ACC
BstXI        1806  CCAGAGAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I         31  GCCGGC               [GA]^CCGG[CT]
EaeI           65  TGGCCG               [CT]^GGCC[GA]
EcoO65I      1186  GGTGACC              G^GT[ACGT]ACC
HaeII          28  GGCGCC               [GA]GCGC^[CT]
HgaI         1267  GCGTC                GACGC(5/10)
KasI           28  GGCGCC               G^GCGCC
MaeI         1996  CTAG                 C^TAG
MfeI         1685  CAATTG               C^AATTG
MunI         1685  CAATTG               C^AATTG
NaeI           31  GCCGGC               GCC^GGC
NarI           28  GGCGCC               GG^CGCC
NgoMIV         31  GCCGGC               G^CCGGC
NspI         1929  ACATGT               [GA]CATG^[CT]
PaeR7I        647  CTCGAG               C^TCGAG
PflFI        1827  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
ScaI         2156  AGTACT               AGT^ACT
SfcI          249  CTATAG               C^T[GA][CT]AG
SfoI           28  GGCGCC               GGC^GCC
Tth111I      1827  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1995  TCTAGA               T^CTAGA
XhoI          647  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AatII         757  GACGTC               GACGT^C
             1488  GACGTC              
AccII           9  CGCG                 CG^CG
               55  CGCG                
AcsI         1381  GAATTT               [GA]^AATT[CT]
             1560  AAATTT              
ApoI         1381  GAATTT               [GA]^AATT[CT]
             1560  AAATTT              
BbrPI         785  CACGTG               CAC^GTG
             1222  CACGTG              
BbsI         2101  GAAGAC               GAAGAC(2/6)
             1833  GTCTTC              
BciVI         579  GGATAC               GTATCC(6/5)
              656  GTATCC              
BclI          491  TGATCA               T^GATCA
             2349  TGATCA              
BlpI          637  GCTGAGC              GC^T[ACGT]AGC
              768  GCTTAGC             
Bpu1102I      637  GCTGAGC              GC^T[ACGT]AGC
              768  GCTTAGC             
BpuAI        2101  GAAGAC               GAAGAC(2/6)
             1833  GTCTTC              
BsaAI         785  CACGTG               [CT]AC^GT[GA]
             1222  CACGTG              
BsmBI         759  CGTCTC               CGTCTC(1/5)
             1876  CGTCTC              
BspHI        1317  TCATGA               T^CATGA
             1419  TCATGA              
BspMI         347  ACCTGC               ACCTGC(4/8)
              417  GCAGGT              
BsrDI        1641  CATTGC               GCAATG(2/0)
             1079  GCAATG              
BstUI           9  CGCG                 CG^CG
               55  CGCG                
CelII         637  GCTGAGC              GC^T[ACGT]AGC
              768  GCTTAGC             
EcoNI        2132  CCTCCTTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2190  CCTCAGAAAGG         
EcoRV        1711  GATATC               GAT^ATC
             1721  GATATC              
FauI          662  CCCGC                CCCGC(4/6)
             1401  GCGGG               
FbaI          491  TGATCA               T^GATCA
             2349  TGATCA              
HincII       2245  GTTAAC               GT[CT]^[GA]AC
             2437  GTTAAC              
HindII       2245  GTTAAC               GT[CT]^[GA]AC
             2437  GTTAAC              
HindIII       766  AAGCTT               A^AGCTT
             1940  AAGCTT              
HpaI         2245  GTTAAC               GTT^AAC
             2437  GTTAAC              
MvnI            9  CGCG                 CG^CG
               55  CGCG                
PmaCI         785  CACGTG               CAC^GTG
             1222  CACGTG              
PmlI          785  CACGTG               CAC^GTG
             1222  CACGTG              
PpuMI         869  GGGACCT              [GA]G^G[AT]CC[CT]
             1147  GGGACCT             
PvuII         962  CAGCTG               CAG^CTG
             1292  CAGCTG              
RcaI         1317  TCATGA               T^CATGA
             1419  TCATGA              


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       Asp700I     AspHI       AvaII       BanII       
BbvI        BcnI        BmyI        BpmI        BsaBI       
BsaHI       BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EarI        EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MamI        
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PflMI       
PleI        RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII       XmnI        
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