Restriction map for pF1KE0686
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspHI       AspI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BcgI        BclI        BfaI        BfrI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsiEI       BsiHKAI     BsmBI       
BspEI       BspMI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstZ17I     Bsu36I      CelII       
CpoI        DraI        DraIII      EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoRI       EcoRV       
FbaI        FseI        FspI        HaeII       HgaI        
Hin1I       HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MroI        MseI        MspA1I      MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        Tru9I       
Tth111I     XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AccII        1090  CGCG                 CG^CG
AflIII        734  ACATGT               A^C[GA][CT]GT
AhdI          733  GACATGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI         1172  GGGCCC               GGGCC^C
AspEI         733  GACATGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         1169  CTCGGG               C^[CT]CG[GA]G
BalI         1079  TGGCCA               TGG^CCA
BanI           33  GGTGCC               G^G[CT][GA]CC
BbsI          825  GAAGAC               GAAGAC(2/6)
BciVI         777  GTATCC               GTATCC(6/5)
BglI         1174  GCCCTGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         239  AGATCT               A^GATCT
BpuAI         825  GAAGAC               GAAGAC(2/6)
BsaI          461  GAGACC               GGTCTC(1/5)
BsiWI         351  CGTACG               C^GTACG
BsmAI         461  GAGAC                GTCTC(1/5)
BsoBI        1169  CTCGGG               C^[CT]CG[GA]G
Bsp1407I      758  TGTACA               T^GTACA
BspDI         178  ATCGAT               AT^CGAT
BspHI         497  TCATGA               T^CATGA
BspLU11I      734  ACATGT               A^CATGT
BsrBI        1039  GAGCGG               CCGCTC(-3/-3)
BsrDI         543  CATTGC               GCAATG(2/0)
BsrFI          30  GCCGGT               [GA]^CCGG[CT]
BsrGI         758  TGTACA               T^GTACA
BstEII        192  GGTCACC              G^GT[ACGT]ACC
BstPI         192  GGTCACC              G^GT[ACGT]ACC
BstUI        1090  CGCG                 CG^CG
BstXI        1070  CCAGTGCCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         239  AGATCT               [GA]^GATC[CT]
Cfr10I         30  GCCGGT               [GA]^CCGG[CT]
ClaI          178  ATCGAT               AT^CGAT
DdeI          566  CTGAG                C^T[ACGT]AG
DrdI          184  GACTACAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          798  CCACGG               C^C[GA][CT]GG
EaeI         1079  TGGCCA               [CT]^GGCC[GA]
Eam1105I      733  GACATGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       192  GGTCACC              G^GT[ACGT]ACC
EcoT22I      1130  ATGCAT               ATGCA^T
HincII       1093  GTTGAC               GT[CT]^[GA]AC
HindII       1093  GTTGAC               GT[CT]^[GA]AC
MaeII         140  ACGT                 A^CGT
MflI          239  AGATCT               [GA]^GATC[CT]
MluNI        1079  TGGCCA               TGG^CCA
MscI         1079  TGGCCA               TGG^CCA
MvnI         1090  CGCG                 CG^CG
NsiI         1130  ATGCAT               ATGCA^T
NspI          734  ACATGT               [GA]CATG^[CT]
PflMI         201  CCAGGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          828  GACTC                GAGTC(4/5)
PstI          154  CTGCAG               CTGCA^G
RcaI          497  TCATGA               T^CATGA
SapI          501  GAAGAGC              GCTCTTC(1/4)
SfcI          154  CTGCAG               C^T[GA][CT]AG
SgrAI          29  CGCCGGTG             C[GA]^CCGG[CT]G
SspBI         758  TGTACA               T^GTACA
Van91I        201  CCAGGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          197  CCAGCCAGGTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         239  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcsI          265  AAATTC               [GA]^AATT[CT]
              853  GAATTT              
AluI          317  AGCT                 AG^CT
             1167  AGCT                
AlwNI         202  CAGGTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              523  CAGTATCTG           
ApoI          265  AAATTC               [GA]^AATT[CT]
              853  GAATTT              
BpmI          692  CTCCAG               CTGGAG(16/14)
             1177  CTGGAG              
BseRI         458  GAGGAG               GAGGAG(10/8)
             1060  GAGGAG              
BsgI          319  CTGCAC               GTGCAG(16/14)
             1006  CTGCAC              
BsmFI         741  GTCCC                GGGAC(10/14)
              991  GTCCC               
BsmI          469  GCATTC               GAATGC(1/-1)
              531  GCATTC              
CfoI           97  GCGC                 GCG^C
             1089  GCGC                
EarI          501  GAAGAG               CTCTTC(1/4)
             1152  GAAGAG              
EcoNI         584  CCTCCAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1023  CCTGAAGGAGG         
HhaI           97  GCGC                 GCG^C
             1089  GCGC                
HinP1I         97  GCGC                 G^CGC
             1089  GCGC                
Ksp632I       501  GAAGAG               CTCTTC(1/4)
             1152  GAAGAG              
MaeIII        193  GTCAC                ^GT[ACGT]AC
              731  GTGAC               
MslI          550  CATCGGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              834  CATCCGAATG          
TaqI          179  TCGA                 T^CGA
              456  TCGA                
Tsp45I        193  GTCAC                ^GT[GC]AC
              731  GTGAC               
Tsp509I       266  AATT                 ^AATT
              854  AATT                
TthHB8I       179  TCGA                 T^CGA
              456  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AciI        AfaI        AlwI        AvaII       BanII       
BbvI        BcnI        BmyI        BsaJI       BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DpnII       DraII       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        MboI        MboII       MnlI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PpuMI       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       StyI        TfiI        
TseI        TspRI       
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