Restriction map for pF1KE0686
 (Linear) (Six-base) MAPSORT of: KIEE0686.seq  Check: 3707  from: 1  to: 1200



KIEE0686.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268    1045    1171    1200 
   Size:        268     777     126      29

  Fragments arranged by size: 

                 777     268     126      29
 
AflIII A'CryG_T

Cuts at:      0     734    1200 
   Size:        734     466
 
AhdI GACnn_n'nnGTC

Cuts at:      0     738    1200 
   Size:        738     462
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     250    1200 
   Size:        250     950
 
AlwNI CAG_nnn'CTG

Cuts at:      0     207     528    1200 
   Size:        207     321     672

  Fragments arranged by size: 

                 672     321     207
 
ApaI G_GGCC'C

Cuts at:      0    1176    1200 
   Size:       1176      24
 
ApoI r'AATT_y

Cuts at:      0     265     853    1200 
   Size:        265     588     347

  Fragments arranged by size: 

                 588     347     265
 
AvaI C'yCGr_G

Cuts at:      0    1169    1200 
   Size:       1169      31
 
BanI G'GyrC_C

Cuts at:      0      33    1200 
   Size:         33    1167
 
BanII G_rGCy'C

Cuts at:      0      28     929    1176    1200 
   Size:         28     901     247      24

  Fragments arranged by size: 

                 901     247      28      24
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     832    1200 
   Size:        832     368
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     788    1200 
   Size:        788     412
 
BfrBI ATG'CAT

Cuts at:      0    1132    1200 
   Size:       1132      68
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1180    1200 
   Size:       1180      20
 
BglII A'GATC_T

Cuts at:      0     239    1200 
   Size:        239     961
 
Bme1580I G_kGCm'C

Cuts at:      0     343    1176    1200 
   Size:        343     833      24

  Fragments arranged by size: 

                 833     343      24
 
BmgBI CAC'GTC

Cuts at:      0     141    1200 
   Size:        141    1059
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     330    1200 
   Size:        330     870
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     677    1198    1200 
   Size:        677     521       2

  Fragments arranged by size: 

                 677     521       2
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     455    1200 
   Size:        455     745
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     473    1075    1200 
   Size:        473     602     125

  Fragments arranged by size: 

                 602     473     125
 
BseYI C'CCAG_C

Cuts at:      0     213     702    1200 
   Size:        213     489     498

  Fragments arranged by size: 

                 498     489     213
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304     991    1200 
   Size:        304     687     209

  Fragments arranged by size: 

                 687     304     209
 
BsiWI C'GTAC_G

Cuts at:      0     351    1200 
   Size:        351     849
 
BsmI GAATG_Cn'

Cuts at:      0     469     531    1200 
   Size:        469      62     669

  Fragments arranged by size: 

                 669     469      62
 
Bsp1286I G_dGCh'C

Cuts at:      0      28     343     929    1176    1200 
   Size:         28     315     586     247      24

  Fragments arranged by size: 

                 586     315     247      28      24
 
BspHI T'CATG_A

Cuts at:      0     497    1200 
   Size:        497     703
 
BsrBI CCG'CTC

Cuts at:      0    1041    1200 
   Size:       1041     159
 
BsrDI GCAATG_nn'

Cuts at:      0     542    1200 
   Size:        542     658
 
BsrFI r'CCGG_y

Cuts at:      0      30    1200 
   Size:         30    1170
 
BsrGI T'GTAC_A

Cuts at:      0     758    1200 
   Size:        758     442
 
BstEII G'GTnAC_C

Cuts at:      0     192    1200 
   Size:        192    1008
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1077    1200 
   Size:       1077     123
 
BstYI r'GATC_y

Cuts at:      0     239    1200 
   Size:        239     961
 
BtgI C'CryG_G

Cuts at:      0     798    1200 
   Size:        798     402
 
BtsI GCAGTG_nn'

Cuts at:      0    1003    1200 
   Size:       1003     197
 
ClaI AT'CG_AT

Cuts at:      0     179    1200 
   Size:        179    1021
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     190    1200 
   Size:        190    1010
 
EaeI y'GGCC_r

Cuts at:      0    1079    1200 
   Size:       1079     121
 
EarI CTCTTCn'nnn_

Cuts at:      0     496    1147    1200 
   Size:        496     651      53

  Fragments arranged by size: 

                 651     496      53
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1173    1200 
   Size:       1173      27
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     677    1045    1171    1198    1200 
   Size:        268     409     368     126      27       2

  Fragments arranged by size: 

                 409     368     268     126      27       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     588    1027    1200 
   Size:        588     439     173

  Fragments arranged by size: 

                 588     439     173
 
EcoO109I rG'GnC_Cy

Cuts at:      0     204     254     990    1020    1173    1200 
   Size:        204      50     736      30     153      27

  Fragments arranged by size: 

                 736     204     153      50      30      27
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     724     756    1200 
   Size:        724      32     444

  Fragments arranged by size: 

                 724     444      32
 
HincII GTy'rAC

Cuts at:      0    1095    1200 
   Size:       1095     105
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     818    1160    1200 
   Size:         32     786     342      40

  Fragments arranged by size: 

                 786     342      40      32
 
MscI TGG'CCA

Cuts at:      0    1081    1200 
   Size:       1081     119
 
MslI CAynn'nnrTG

Cuts at:      0     554     838    1200 
   Size:        554     284     362

  Fragments arranged by size: 

                 554     362     284
 
NsiI A_TGCA'T

Cuts at:      0    1134    1200 
   Size:       1134      66
 
NspI r_CATG'y

Cuts at:      0     738    1200 
   Size:        738     462
 
PasI CC'CwG_GG

Cuts at:      0      15     744    1200 
   Size:         15     729     456

  Fragments arranged by size: 

                 729     456      15
 
PciI A'CATG_T

Cuts at:      0     734    1200 
   Size:        734     466
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     207    1200 
   Size:        207     993
 
PfoI T'CCnGG_A

Cuts at:      0     449    1200 
   Size:        449     751
 
PpuMI rG'GwC_Cy

Cuts at:      0     204     254     990    1020    1200 
   Size:        204      50     736      30     180

  Fragments arranged by size: 

                 736     204     180      50      30
 
PspOMI G'GGCC_C

Cuts at:      0    1172    1200 
   Size:       1172      28
 
PstI C_TGCA'G

Cuts at:      0     158    1200 
   Size:        158    1042
 
SapI GCTCTTCn'nnn_

Cuts at:      0     496    1200 
   Size:        496     704
 
SfcI C'TryA_G

Cuts at:      0     154    1200 
   Size:        154    1046
 
SgrAI Cr'CCGG_yG

Cuts at:      0      30    1200 
   Size:         30    1170
 
StyI C'CwwG_G

Cuts at:      0     296     632     967    1076    1200 
   Size:        296     336     335     109     124

  Fragments arranged by size: 

                 336     335     296     124     109
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     736     946    1200 
   Size:        736     210     254

  Fragments arranged by size: 

                 736     254     210
 
TatI w'GTAC_w

Cuts at:      0     758    1200 
   Size:        758     442
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     130     162    1200 
   Size:        130      32    1038

  Fragments arranged by size: 

                1038     130      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     204    1200 
   Size:        204     996
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     AlfI    AlwNI     ApaI     ApoI     AvaI
     BanI    BanII     BbsI    BciVI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmrI     BpmI     BsaI    BseRI    BseYI     BsgI    BsiWI
     BsmI Bsp1286I    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BstEII
    BstXI    BstYI     BtgI     BtsI     ClaI     DrdI     EaeI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    Hin4I   HincII     MmeI     MscI
     MslI     NsiI     NspI     PasI     PciI    PflMI     PfoI    PpuMI
   PspOMI     PstI     SapI     SfcI    SgrAI     StyI    TaqII     TatI
     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AleI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BbvCI     BcgI     BclI     BlpI     BmtI     BplI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI
  BsiHKAI    BsmBI    BspEI    BspMI   BssHII    BssSI   BstAPI    BstBI
  BstZ17I   Bsu36I    BtgZI    CspCI     DraI   DraIII     EagI  EcoICRI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI
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