################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## Acc65I AclI AflII AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AvrII BbeI BfaI BfrI BlnI BsaBI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrGI BssHII Bst1107I BstZ17I ClaI CpoI DraI Eam1105I EcoRI EcoRV EcoT22I FseI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI NspI PacI PaeR7I PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 475 GACGTC GACGT^C AccIII 2003 TCCGGA T^CCGGA AcsI 743 AAATTC [GA]^AATT[CT] AfaI 624 GTAC GT^AC AgeI 1652 ACCGGT A^CCGGT ApaI 2093 GGGCCC GGGCC^C ApoI 743 AAATTC [GA]^AATT[CT] AspHI 1036 GAGCTC G[AT]GC[AT]^C AviII 296 TGCGCA TGC^GCA BalI 1614 TGGCCA TGG^CCA BbvCI 2254 GCTGAGG CCTCAGC(-5/-2) BcgI 299 GCACCCCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2369 GGATAC GTATCC(6/5) BglII 311 AGATCT A^GATCT BlpI 114 GCTCAGC GC^T[ACGT]AGC Bpu1102I 114 GCTCAGC GC^T[ACGT]AGC BsaI 1171 GAGACC GGTCTC(1/5) BseAI 2003 TCCGGA T^CCGGA BsiEI 704 CGGCCG CG[GA][CT]^CG BsiHKAI 1036 GAGCTC G[AT]GC[AT]^C BspEI 2003 TCCGGA T^CCGGA BsrDI 1719 CATTGC GCAATG(2/0) BstAPI 1929 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 137 TTCGAA TT^CGAA CelII 114 GCTCAGC GC^T[ACGT]AGC DraIII 588 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2137 GACCTGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 704 CGGCCG C^GGCCG EclXI 704 CGGCCG C^GGCCG Eco52I 704 CGGCCG C^GGCCG FspI 296 TGCGCA TGC^GCA MluNI 1614 TGGCCA TGG^CCA MroI 2003 TCCGGA T^CCGGA MscI 1614 TGGCCA TGG^CCA NaeI 1210 GCCGGC GCC^GGC NcoI 102 CCATGG C^CATGG NgoMIV 1210 GCCGGC G^CCGGC NspV 137 TTCGAA TT^CGAA PinAI 1652 ACCGGT A^CCGGT PpuMI 2135 AGGACCT [GA]G^G[AT]CC[CT] PshAI 1233 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 466 CTGCAG CTGCA^G PvuII 2023 CAGCTG CAG^CTG RsaI 624 GTAC GT^AC SacI 1036 GAGCTC GAGCT^C SfuI 137 TTCGAA TT^CGAA SmlI 375 CTCAAG C^T[CT][GA]AG StuI 260 AGGCCT AGG^CCT Tsp509I 744 AATT ^AATT ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccI 931 GTCTAC GT^[AC][GT]AC 1507 GTAGAC AflIII 291 ACACGT A^C[GA][CT]GT 852 ACACGT AocI 1073 CCTCAGG CC^T[ACGT]AGG 1902 CCTGAGG Asp700I 597 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1413 GAAGCACTTC AvaI 2090 CTCGGG C^[CT]CG[GA]G 2179 CCCGAG BamHI 323 GGATCC G^GATCC 2238 GGATCC BanI 559 GGCACC G^G[CT][GA]CC 2055 GGTGCC BbrPI 292 CACGTG CAC^GTG 853 CACGTG BclI 237 TGATCA T^GATCA 434 TGATCA BglI 531 GCCACTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 635 GCCTGGTGGGC BpmI 109 CTCCAG CTGGAG(16/14) 2026 CTGGAG BsaAI 292 CACGTG [CT]AC^GT[GA] 853 CACGTG BsgI 763 CTGCAC GTGCAG(16/14) 1480 GTGCAG BsoBI 2090 CTCGGG C^[CT]CG[GA]G 2179 CCCGAG BspHI 341 TCATGA T^CATGA 2285 TCATGA BspMI 680 ACCTGC ACCTGC(4/8) 2138 ACCTGC BsrFI 1210 GCCGGC [GA]^CCGG[CT] 1652 ACCGGT BssSI 155 CACGAG CACGAG(-5/-1) 58 CTCGTG BstEII 198 GGTCACC G^GT[ACGT]ACC 1624 GGTCACC BstPI 198 GGTCACC G^GT[ACGT]ACC 1624 GGTCACC BstXI 503 CCATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1754 CCATCGAGCTGG Bsu36I 1073 CCTCAGG CC^T[ACGT]AGG 1902 CCTGAGG Cfr10I 1210 GCCGGC [GA]^CCGG[CT] 1652 ACCGGT Eco81I 1073 CCTCAGG CC^T[ACGT]AGG 1902 CCTGAGG EcoNI 432 CCTGATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 825 CCTCTTTGAGG EcoO65I 198 GGTCACC G^GT[ACGT]ACC 1624 GGTCACC FbaI 237 TGATCA T^GATCA 434 TGATCA MseI 221 TTAA T^TAA 1658 TTAA MspA1I 1930 CAGCGG C[AC]G^C[GT]G 2023 CAGCTG PflMI 1731 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2064 CCACAAGCTGG PmaCI 292 CACGTG CAC^GTG 853 CACGTG PmlI 292 CACGTG CAC^GTG 853 CACGTG RcaI 341 TCATGA T^CATGA 2285 TCATGA SexAI 1297 ACCTGGT A^CC[AT]GGT 2192 ACCTGGT SfcI 466 CTGCAG C^T[GA][CT]AG 1008 CTACAG Tru9I 221 TTAA T^TAA 1658 TTAA Van91I 1731 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2064 CCACAAGCTGG XcmI 1493 CCATTCGGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1754 CCATCGAGCTGGTGG XmnI 597 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1413 GAAGCACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AlwNI Aor51HI AspI AvaII BanII BbsI BbvI BcnI BmyI BpuAI BsaHI BsaJI BsaWI BseRI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflFI PleI SapI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp45I TspRI Tth111I TthHB8I XhoII