(Linear) (Six-base) MAPSORT of: KIEE2691.seq Check: 3272 from: 1 to: 2445 KIEE2691.orf With 182 enzymes: SgfI * June 2, 2017 11:42 .. AatII G_ACGT'C Cuts at: 0 479 2445 Size: 479 1966 AccI GT'mk_AC Cuts at: 0 932 1508 2445 Size: 932 576 937 Fragments arranged by size: 937 932 576 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1168 2230 2445 Size: 1168 1062 215 Fragments arranged by size: 1168 1062 215 AfeI AGC'GCT Cuts at: 0 1546 1699 2377 2445 Size: 1546 153 678 68 Fragments arranged by size: 1546 678 153 68 AflIII A'CryG_T Cuts at: 0 291 852 2445 Size: 291 561 1593 Fragments arranged by size: 1593 561 291 AgeI A'CCGG_T Cuts at: 0 1652 2445 Size: 1652 793 AleI CACnn'nnGTG Cuts at: 0 292 1960 2445 Size: 292 1668 485 Fragments arranged by size: 1668 485 292 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2305 2445 Size: 2305 140 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2414 2445 Size: 2414 31 AlwNI CAG_nnn'CTG Cuts at: 0 1578 1935 1998 2445 Size: 1578 357 63 447 Fragments arranged by size: 1578 447 357 63 ApaI G_GGCC'C Cuts at: 0 2097 2445 Size: 2097 348 ApoI r'AATT_y Cuts at: 0 743 2445 Size: 743 1702 AvaI C'yCGr_G Cuts at: 0 2090 2179 2445 Size: 2090 89 266 Fragments arranged by size: 2090 266 89 BamHI G'GATC_C Cuts at: 0 323 2238 2445 Size: 323 1915 207 Fragments arranged by size: 1915 323 207 BanI G'GyrC_C Cuts at: 0 559 2055 2445 Size: 559 1496 390 Fragments arranged by size: 1496 559 390 BanII G_rGCy'C Cuts at: 0 1040 1712 2097 2445 Size: 1040 672 385 348 Fragments arranged by size: 1040 672 385 348 BbsI GAAGACnn'nnnn_ Cuts at: 0 471 993 1610 1927 2322 2445 Size: 471 522 617 317 395 123 Fragments arranged by size: 617 522 471 395 317 123 BbvCI CC'TCA_GC Cuts at: 0 2255 2445 Size: 2255 190 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 288 322 2445 Size: 288 34 2123 Fragments arranged by size: 2123 288 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2363 2445 Size: 2363 82 BclI T'GATC_A Cuts at: 0 237 434 2445 Size: 237 197 2011 Fragments arranged by size: 2011 237 197 BglI GCCn_nnn'nGGC Cuts at: 0 537 641 2445 Size: 537 104 1804 Fragments arranged by size: 1804 537 104 BglII A'GATC_T Cuts at: 0 311 2445 Size: 311 2134 BlpI GC'TnA_GC Cuts at: 0 115 2445 Size: 115 2330 Bme1580I G_kGCm'C Cuts at: 0 562 589 2097 2445 Size: 562 27 1508 348 Fragments arranged by size: 1508 562 348 27 BmrI ACTGGGnnnn_n' Cuts at: 0 2339 2445 Size: 2339 106 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 2047 2445 Size: 94 1953 398 Fragments arranged by size: 1953 398 94 Bpu10I CC'TnA_GC Cuts at: 0 886 2255 2405 2445 Size: 886 1369 150 40 Fragments arranged by size: 1369 886 150 40 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 360 2445 Size: 360 2085 BsaI GGTCTCn'nnnn_ Cuts at: 0 1165 2445 Size: 1165 1280 BsaAI yAC'GTr Cuts at: 0 294 855 2445 Size: 294 561 1590 Fragments arranged by size: 1590 561 294 BsaHI Gr'CG_yC Cuts at: 0 476 800 1196 2445 Size: 476 324 396 1249 Fragments arranged by size: 1249 476 396 324 BsaWI w'CCGG_w Cuts at: 0 192 1652 2003 2445 Size: 192 1460 351 442 Fragments arranged by size: 1460 442 351 192 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 171 201 2293 2323 2445 Size: 171 30 2092 30 122 Fragments arranged by size: 2092 171 122 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 847 1897 1900 2218 2272 2445 Size: 847 1050 3 318 54 173 Fragments arranged by size: 1050 847 318 173 54 3 BseYI C'CCAG_C Cuts at: 0 1278 1287 1712 2356 2445 Size: 1278 9 425 644 89 Fragments arranged by size: 1278 644 425 89 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 748 1501 2445 Size: 748 753 944 Fragments arranged by size: 944 753 748 BsiEI CG_ry'CG Cuts at: 0 707 2445 Size: 707 1738 BsiHKAI G_wGCw'C Cuts at: 0 1040 2445 Size: 1040 1405 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1203 1531 1806 1815 2445 Size: 1203 328 275 9 630 Fragments arranged by size: 1203 630 328 275 9 Bsp1286I G_dGCh'C Cuts at: 0 562 589 1040 1712 2097 2445 Size: 562 27 451 672 385 348 Fragments arranged by size: 672 562 451 385 348 27 BspEI T'CCGG_A Cuts at: 0 2003 2445 Size: 2003 442 BspHI T'CATG_A Cuts at: 0 341 2285 2445 Size: 341 1944 160 Fragments arranged by size: 1944 341 160 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 689 2147 2445 Size: 689 1458 298 Fragments arranged by size: 1458 689 298 BsrBI CCG'CTC Cuts at: 0 187 993 1772 2445 Size: 187 806 779 673 Fragments arranged by size: 806 779 673 187 BsrDI GCAATG_nn' Cuts at: 0 1718 2445 Size: 1718 727 BsrFI r'CCGG_y Cuts at: 0 1210 1652 2445 Size: 1210 442 793 Fragments arranged by size: 1210 793 442 BssSI C'ACGA_G Cuts at: 0 58 155 2445 Size: 58 97 2290 Fragments arranged by size: 2290 97 58 BstAPI GCAn_nnn'nTGC Cuts at: 0 1935 2445 Size: 1935 510 BstBI TT'CG_AA Cuts at: 0 138 2445 Size: 138 2307 BstEII G'GTnAC_C Cuts at: 0 198 1624 2445 Size: 198 1426 821 Fragments arranged by size: 1426 821 198 BstXI CCAn_nnnn'nTGG Cuts at: 0 510 1761 2445 Size: 510 1251 684 Fragments arranged by size: 1251 684 510 BstYI r'GATC_y Cuts at: 0 41 311 323 1562 1787 1889 2238 2445 Size: 41 270 12 1239 225 102 349 207 Fragments arranged by size: 1239 349 270 225 207 102 41 12 Bsu36I CC'TnA_GG Cuts at: 0 1074 1903 2445 Size: 1074 829 542 Fragments arranged by size: 1074 829 542 BtgI C'CryG_G Cuts at: 0 102 578 707 716 1202 2445 Size: 102 476 129 9 486 1243 Fragments arranged by size: 1243 486 476 129 102 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 927 1251 1338 1365 2445 Size: 927 324 87 27 1080 Fragments arranged by size: 1080 927 324 87 27 BtsI GCAGTG_nn' Cuts at: 0 532 1442 2445 Size: 532 910 1003 Fragments arranged by size: 1003 910 532 DraIII CAC_nnn'GTG Cuts at: 0 593 2445 Size: 593 1852 DrdI GACnn_nn'nnGTC Cuts at: 0 2143 2445 Size: 2143 302 EaeI y'GGCC_r Cuts at: 0 704 1208 1614 2445 Size: 704 504 406 831 Fragments arranged by size: 831 704 504 406 EagI C'GGCC_G Cuts at: 0 704 2445 Size: 704 1741 EarI CTCTTCn'nnn_ Cuts at: 0 493 691 1000 1045 1261 1553 2445 Size: 493 198 309 45 216 292 892 Fragments arranged by size: 892 493 309 292 216 198 45 EcoICRI GAG'CTC Cuts at: 0 1038 2445 Size: 1038 1407 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 1168 2047 2230 2445 Size: 94 1074 879 183 215 Fragments arranged by size: 1074 879 215 183 94 EcoNI CCTnn'n_nnAGG Cuts at: 0 436 829 2445 Size: 436 393 1616 Fragments arranged by size: 1616 436 393 EcoO109I rG'GnC_Cy Cuts at: 0 429 1365 2136 2445 Size: 429 936 771 309 Fragments arranged by size: 936 771 429 309 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1434 2445 Size: 1434 1011 FspI TGC'GCA Cuts at: 0 298 2445 Size: 298 2147 FspAI rTGC'GCAy Cuts at: 0 298 2445 Size: 298 2147 HaeII r_GCGC'y Cuts at: 0 1066 1548 1701 2379 2445 Size: 1066 482 153 678 66 Fragments arranged by size: 1066 678 482 153 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1364 1396 2223 2255 2293 2325 2445 Size: 1364 32 827 32 38 32 120 Fragments arranged by size: 1364 827 120 38 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 497 2445 Size: 497 1948 MscI TGG'CCA Cuts at: 0 1616 2445 Size: 1616 829 MslI CAynn'nnrTG Cuts at: 0 292 752 1960 2287 2445 Size: 292 460 1208 327 158 Fragments arranged by size: 1208 460 327 292 158 MspA1I CmG'CkG Cuts at: 0 1932 2025 2445 Size: 1932 93 420 Fragments arranged by size: 1932 420 93 NaeI GCC'GGC Cuts at: 0 1212 2445 Size: 1212 1233 NcoI C'CATG_G Cuts at: 0 102 2445 Size: 102 2343 NgoMIV G'CCGG_C Cuts at: 0 1210 2445 Size: 1210 1235 PasI CC'CwG_GG Cuts at: 0 906 2445 Size: 906 1539 PflMI CCAn_nnn'nTGG Cuts at: 0 1737 2070 2445 Size: 1737 333 375 Fragments arranged by size: 1737 375 333 PfoI T'CCnGG_A Cuts at: 0 386 509 671 1403 1577 2445 Size: 386 123 162 732 174 868 Fragments arranged by size: 868 732 386 174 162 123 PmlI CAC'GTG Cuts at: 0 294 855 2445 Size: 294 561 1590 Fragments arranged by size: 1590 561 294 PpuMI rG'GwC_Cy Cuts at: 0 2136 2445 Size: 2136 309 PshAI GACnn'nnGTC Cuts at: 0 1237 2445 Size: 1237 1208 PspOMI G'GGCC_C Cuts at: 0 2093 2445 Size: 2093 352 PstI C_TGCA'G Cuts at: 0 470 2445 Size: 470 1975 PvuII CAG'CTG Cuts at: 0 2025 2445 Size: 2025 420 SacI G_AGCT'C Cuts at: 0 1040 2445 Size: 1040 1405 SapI GCTCTTCn'nnn_ Cuts at: 0 691 1000 1045 1261 2445 Size: 691 309 45 216 1184 Fragments arranged by size: 1184 691 309 216 45 SexAI A'CCwGG_T Cuts at: 0 1297 2192 2445 Size: 1297 895 253 Fragments arranged by size: 1297 895 253 SfcI C'TryA_G Cuts at: 0 466 1008 2445 Size: 466 542 1437 Fragments arranged by size: 1437 542 466 SmlI C'TyrA_G Cuts at: 0 375 2445 Size: 375 2070 StuI AGG'CCT Cuts at: 0 262 2445 Size: 262 2183 StyI C'CwwG_G Cuts at: 0 102 1337 1703 1871 2435 2445 Size: 102 1235 366 168 564 10 Fragments arranged by size: 1235 564 366 168 102 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2094 2445 Size: 2094 351 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 171 203 446 478 1819 1851 2445 Size: 171 32 243 32 1341 32 594 Fragments arranged by size: 1341 594 243 171 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 802 2307 2326 2445 Size: 802 1505 19 119 Fragments arranged by size: 1505 802 119 19 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1500 1761 2445 Size: 1500 261 684 Fragments arranged by size: 1500 684 261 XmnI GAAnn'nnTTC Cuts at: 0 601 1417 2445 Size: 601 816 1028 Fragments arranged by size: 1028 816 601 ZraI GAC'GTC Cuts at: 0 477 2445 Size: 477 1968 Enzymes that do cut: AatII AccI AcuI AfeI AflIII AgeI AleI AlfI AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SacI SapI SexAI SfcI SmlI StuI StyI TaqII TstI Tth111I XcmI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AflII AhdI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BfrBI BmgBI BmtI BplI BsaBI BsiWI BsmI BsrGI BssHII BstZ17I ClaI CspCI DraI EciI EcoRI EcoRV FseI HincII HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI XbaI XhoI XmaI