Restriction map for pF1KE3105
##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

Acc65I      AccI        AccIII      AciI        AclI        
AflII       AgeI        AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AvaII       AviII       AvrII       BalI        BamHI       
BbsI        BcgI        BciVI       BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BsaI        BsaWI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FseI        FspI        HincII      
HindII      HindIII     HpaI        HphI        KpnI        
Ksp632I     KspI        MaeI        MaeIII      MamI        
MboII       MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MunI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PflFI       PflMI       
PinAI       PmeI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
StuI        StyI        SwaI        Tsp45I      Tth111I     
Van91I      XbaI        XcmI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AatII         399  GACGTC               GACGT^C
AccII         768  CGCG                 CG^CG
AcsI          208  GAATTT               [GA]^AATT[CT]
AfaI          750  GTAC                 GT^AC
AflIII        308  ACACGT               A^C[GA][CT]GT
AlwNI          43  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          375  GGGCCC               GGGCC^C
ApoI          208  GAATTT               [GA]^AATT[CT]
Asp700I       472  GAATTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          124  CTCGAG               C^[CT]CG[GA]G
BbeI          691  GGCGCC               GGCGC^C
BbrPI         309  CACGTG               CAC^GTG
BbvCI         756  GCTGAGG              CCTCAGC(-5/-2)
BclI          239  TGATCA               T^GATCA
BpmI          655  CTCCAG               CTGGAG(16/14)
BsgI          430  GTGCAG               GTGCAG(16/14)
BsmFI         637  GTCCC                GGGAC(10/14)
BsoBI         124  CTCGAG               C^[CT]CG[GA]G
BsrFI         668  ACCGGC               [GA]^CCGG[CT]
BstAPI        727  GCAGCCTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         114  TTCGAA               TT^CGAA
BstF5I        339  CATCC                GGATG(2/0)
BstUI         768  CGCG                 CG^CG
Cfr10I        668  ACCGGC               [GA]^CCGG[CT]
DpnII         240  GATC                 ^GATC
EcoNI         163  CCTTATGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          239  TGATCA               T^GATCA
FokI          339  CATCC                GGATG(9/13)
HaeII         691  GGCGCC               [GA]GCGC^[CT]
HgaI          582  GACGC                GACGC(5/10)
KasI          691  GGCGCC               G^GCGCC
MboI          240  GATC                 ^GATC
MspA1I        607  CAGCTG               C[AC]G^C[GT]G
MvnI          768  CGCG                 CG^CG
NarI          691  GGCGCC               GG^CGCC
NdeII         240  GATC                 ^GATC
NspI          621  GCATGC               [GA]CATG^[CT]
NspV          114  TTCGAA               TT^CGAA
PaeR7I        124  CTCGAG               C^TCGAG
PmaCI         309  CACGTG               CAC^GTG
PmlI          309  CACGTG               CAC^GTG
PvuII         607  CAGCTG               CAG^CTG
RsaI          750  GTAC                 GT^AC
Sau3AI        240  GATC                 ^GATC
SfaNI         764  GCATC                GCATC(5/9)
SfoI          691  GGCGCC               GGC^GCC
SfuI          114  TTCGAA               TT^CGAA
SmlI          124  CTCGAG               C^T[CT][GA]AG
SphI          621  GCATGC               GCATG^C
SspI          118  AATATT               AAT^ATT
TfiI          603  GATTC                G^A[AT]TC
TspRI         428  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoI          124  CTCGAG               C^TCGAG
XmnI          472  GAATTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AcyI          399  GACGTC               G[GA]^CG[CT]C
              691  GGCGCC              
AluI          358  AGCT                 AG^CT
              608  AGCT                
BsaAI         309  CACGTG               [CT]AC^GT[GA]
              751  TACGTG              
BsaHI         399  GACGTC               G[GA]^CG[CT]C
              691  GGCGCC              
BseRI         493  GAGGAG               GAGGAG(10/8)
              759  GAGGAG              
BsmBI         397  GAGACG               CGTCTC(1/5)
              580  GAGACG              
Cac8I         621  GCATGC               GC[ACGT]^[ACGT]GC
              660  GCAGGC              
DraII         374  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              375  GGGCCCC             
DsaI           32  CCACGG               C^C[GA][CT]GG
              675  CCACGG              
EcoO109I      374  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              375  GGGCCCC             
Hin1I         399  GACGTC               G[GA]^CG[CT]C
              691  GGCGCC              
PleI          546  GACTC                GAGTC(4/5)
              680  GACTC               
TaqI          115  TCGA                 T^CGA
              125  TCGA                
TthHB8I       115  TCGA                 T^CGA
              125  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

BanI        BanII       BbvI        BcnI        BmyI        
BsaJI       BsiYI       BslI        BsmAI       Bsp1286I    
BsrI        BssKI       BstNI       CfoI        Cfr13I      
DdeI        EcoRII      Fnu4HI      HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       ItaI        
MaeII       MnlI        MseI        MspI        MvaI        
MwoI        NciI        NlaIII      NlaIV       Sau96I      
ScrFI       Tru9I       TseI        Tsp509I     
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