Restriction map for pF1KE3105
 (Linear) (Six-base) MAPSORT of: KIEE3105.seq  Check: 7219  from: 1  to: 777



KIEE3105.orf

 With 182 enzymes: SgfI * 

                        July 3, 2020 20:21  ..

AatII G_ACGT'C

Cuts at:      0     403     777 
   Size:        403     374
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     503     777 
   Size:         70     433     274

  Fragments arranged by size: 

                 433     274      70
 
AflIII A'CryG_T

Cuts at:      0     308     777 
   Size:        308     469
 
AlwNI CAG_nnn'CTG

Cuts at:      0      48     777 
   Size:         48     729
 
ApaI G_GGCC'C

Cuts at:      0     379     777 
   Size:        379     398
 
ApoI r'AATT_y

Cuts at:      0     208     777 
   Size:        208     569
 
AvaI C'yCGr_G

Cuts at:      0     124     777 
   Size:        124     653
 
BanI G'GyrC_C

Cuts at:      0      84     671     691     777 
   Size:         84     587      20      86

  Fragments arranged by size: 

                 587      86      84      20
 
BanII G_rGCy'C

Cuts at:      0      27     107     353     379     656     777 
   Size:         27      80     246      26     277     121

  Fragments arranged by size: 

                 277     246     121      80      27      26
 
BbeI G_GCGC'C

Cuts at:      0     695     777 
   Size:        695      82
 
BbvCI CC'TCA_GC

Cuts at:      0     757     777 
   Size:        757      20
 
BclI T'GATC_A

Cuts at:      0     239     777 
   Size:        239     538
 
Bme1580I G_kGCm'C

Cuts at:      0     379     777 
   Size:        379     398
 
BmgBI CAC'GTC

Cuts at:      0     564     777 
   Size:        564     213
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     221     356     777 
   Size:        221     135     421

  Fragments arranged by size: 

                 421     221     135
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     640     777 
   Size:        640     137
 
Bpu10I CC'TnA_GC

Cuts at:      0     355     757     777 
   Size:        355     402      20

  Fragments arranged by size: 

                 402     355      20
 
BsaAI yAC'GTr

Cuts at:      0     311     753     777 
   Size:        311     442      24

  Fragments arranged by size: 

                 442     311      24
 
BsaHI Gr'CG_yC

Cuts at:      0     400     692     777 
   Size:        400     292      85

  Fragments arranged by size: 

                 400     292      85
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     508     774     777 
   Size:        508     266       3

  Fragments arranged by size: 

                 508     266       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     451     777 
   Size:        451     326
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     391     574     777 
   Size:        391     183     203

  Fragments arranged by size: 

                 391     203     183
 
Bsp1286I G_dGCh'C

Cuts at:      0      27     107     353     379     656     777 
   Size:         27      80     246      26     277     121

  Fragments arranged by size: 

                 277     246     121      80      27      26
 
BsrFI r'CCGG_y

Cuts at:      0     668     777 
   Size:        668     109
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     733     777 
   Size:        733      44
 
BstBI TT'CG_AA

Cuts at:      0     115     777 
   Size:        115     662
 
BtgI C'CryG_G

Cuts at:      0      32     675     777 
   Size:         32     643     102

  Fragments arranged by size: 

                 643     102      32
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     750     777 
   Size:        750      27
 
BtsI GCAGTG_nn'

Cuts at:      0     434     777 
   Size:        434     343
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     503     640     777 
   Size:         70     433     137     137

  Fragments arranged by size: 

                 433     137     137      70
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     167     777 
   Size:        167     610
 
EcoO109I rG'GnC_Cy

Cuts at:      0     375     376     777 
   Size:        375       1     401

  Fragments arranged by size: 

                 401     375       1
 
HaeII r_GCGC'y

Cuts at:      0     695     777 
   Size:        695      82
 
KasI G'GCGC_C

Cuts at:      0     691     777 
   Size:        691      86
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     383     777 
   Size:         52     331     394

  Fragments arranged by size: 

                 394     331      52
 
MspA1I CmG'CkG

Cuts at:      0     609     777 
   Size:        609     168
 
NarI GG'CG_CC

Cuts at:      0     692     777 
   Size:        692      85
 
NspI r_CATG'y

Cuts at:      0     625     777 
   Size:        625     152
 
PasI CC'CwG_GG

Cuts at:      0     384     777 
   Size:        384     393
 
PmlI CAC'GTG

Cuts at:      0     311     777 
   Size:        311     466
 
PspOMI G'GGCC_C

Cuts at:      0     375     777 
   Size:        375     402
 
PvuII CAG'CTG

Cuts at:      0     609     777 
   Size:        609     168
 
SfoI GGC'GCC

Cuts at:      0     693     777 
   Size:        693      84
 
SmlI C'TyrA_G

Cuts at:      0     124     777 
   Size:        124     653
 
SphI G_CATG'C

Cuts at:      0     625     777 
   Size:        625     152
 
SspI AAT'ATT

Cuts at:      0     120     777 
   Size:        120     657
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     676     777 
   Size:        676     101
 
XhoI C'TCGA_G

Cuts at:      0     124     777 
   Size:        124     653
 
XmnI GAAnn'nnTTC

Cuts at:      0     476     777 
   Size:        476     301
 
ZraI GAC'GTC

Cuts at:      0     401     777 
   Size:        401     376
 

 Enzymes that do cut:

    AatII     AcuI   AflIII    AlwNI     ApaI     ApoI     AvaI     BanI
    BanII     BbeI    BbvCI     BclI Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BsaAI    BsaHI    BseRI     BsgI    BsmBI Bsp1286I    BsrFI
   BstAPI    BstBI     BtgI    BtgZI     BtsI  Eco57MI    EcoNI EcoO109I
    HaeII     KasI     MmeI   MspA1I     NarI     NspI     PasI     PmlI
   PspOMI    PvuII     SfoI     SmlI     SphI     SspI    TaqII     XhoI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbsI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI     BmtI     BplI    BpuEI     BsaI    BsaBI    BsaWI    BsaXI
    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MscI     MslI     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PfoI     PmeI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI
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