Restriction map for pF1KE2538
##################################
# NO CUT ENZYMES (   58 enzymes) #
##################################

AatII       Acc65I      AcyI        AflII       AgeI        
Alw44I      Aor51HI     ApaLI       AscI        Asp718I     
AspI        AviII       AvrII       BbeI        BcgI        
BciVI       BfrI        BglI        BlnI        BsaHI       
BsiWI       BssHII      Bst1107I    BstZ17I     CpoI        
DraIII      Eco47III    FseI        FspI        HaeII       
Hin1I       KasI        KpnI        KspI        MluI        
NarI        NheI        NotI        NruI        PacI        
PflFI       PinAI       PmeI        PvuI        RsrII       
SacII       SalI        SbfI        SfiI        SfoI        
SgrAI       SmaI        SnaBI       Sse8387I    SwaI        
Tth111I     XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccIII       3420  TCCGGA               T^CCGGA
AclI         4986  AACGTT               AA^CGTT
AhdI         4087  GACAAGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI         4336  GGGCCC               GGGCC^C
AspEI        4087  GACAAGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         6023  TGGCCA               TGG^CCA
BbrPI        3887  CACGTG               CAC^GTG
BbvCI        7081  CCTCAGC              CCTCAGC(-5/-2)
BseAI        3420  TCCGGA               T^CCGGA
BsiEI        5364  CGGCCG               CG[GA][CT]^CG
BsmBI        3193  CGTCTC               CGTCTC(1/5)
BspDI        1856  ATCGAT               AT^CGAT
BspEI        3420  TCCGGA               T^CCGGA
BsrBI        6265  GAGCGG               CCGCTC(-3/-3)
BsrFI        6777  GCCGGC               [GA]^CCGG[CT]
BssSI        1364  CACGAG               CACGAG(-5/-1)
BstAPI       6414  GCAACAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        4713  TTCGAA               TT^CGAA
CfoI         1332  GCGC                 GCG^C
Cfr10I       6777  GCCGGC               [GA]^CCGG[CT]
ClaI         1856  ATCGAT               AT^CGAT
DrdI         6896  GACAGACATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         5364  CGGCCG               C^GGCCG
Eam1105I     4087  GACAAGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        5364  CGGCCG               C^GGCCG
Eco52I       5364  CGGCCG               C^GGCCG
EcoRV        4189  GATATC               GAT^ATC
HhaI         1332  GCGC                 GCG^C
HinP1I       1332  GCGC                 G^CGC
HincII       4983  GTTAAC               GT[CT]^[GA]AC
HindII       4983  GTTAAC               GT[CT]^[GA]AC
HindIII      5383  AAGCTT               A^AGCTT
HpaI         4983  GTTAAC               GTT^AAC
MluNI        6023  TGGCCA               TGG^CCA
MroI         3420  TCCGGA               T^CCGGA
MscI         6023  TGGCCA               TGG^CCA
NaeI         6777  GCCGGC               GCC^GGC
NdeI         7328  CATATG               CA^TATG
NgoMIV       6777  GCCGGC               G^CCGGC
NspV         4713  TTCGAA               TT^CGAA
PflMI        6714  CCAGTATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        3887  CACGTG               CAC^GTG
PmlI         3887  CACGTG               CAC^GTG
Psp1406I     4986  AACGTT               AA^CGTT
SfuI         4713  TTCGAA               TT^CGAA
SpeI         2575  ACTAGT               A^CTAGT
Van91I       6714  CCAGTATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1237  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccII        1331  CGCG                 CG^CG
             5546  CGCG                
AocI         1446  CCTAAGG              CC^T[ACGT]AGG
             7579  CCTGAGG             
AseI         1807  ATTAAT               AT^TAAT
             5920  ATTAAT              
AsnI         1807  ATTAAT               AT^TAAT
             5920  ATTAAT              
Asp700I       868  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5028  GAAATGTTTC          
AvaI         4772  CTCGAG               C^[CT]CG[GA]G
             7193  CTCGAG              
BanI          172  GGCACC               G^G[CT][GA]CC
             6675  GGTGCC              
BglII         706  AGATCT               A^GATCT
             1210  AGATCT              
BsaWI        3420  TCCGGA               [AT]^CCGG[AT]
             7466  ACCGGA              
BsmI         4487  GAATGC               GAATGC(1/-1)
             5803  GAATGC              
BsoBI        4772  CTCGAG               C^[CT]CG[GA]G
             7193  CTCGAG              
BstUI        1331  CGCG                 CG^CG
             5546  CGCG                
Bsu36I       1446  CCTAAGG              CC^T[ACGT]AGG
             7579  CCTGAGG             
DsaI         3023  CCATGG               C^C[GA][CT]GG
             3236  CCATGG              
Eco81I       1446  CCTAAGG              CC^T[ACGT]AGG
             7579  CCTGAGG             
EcoT22I      2139  ATGCAT               ATGCA^T
             3050  ATGCAT              
FauI         6477  CCCGC                CCCGC(4/6)
             6267  GCGGG               
HgaI         1329  GACGC                GACGC(5/10)
             5869  GACGC               
MfeI         5049  CAATTG               C^AATTG
             6953  CAATTG              
MunI         5049  CAATTG               C^AATTG
             6953  CAATTG              
MvnI         1331  CGCG                 CG^CG
             5546  CGCG                
NcoI         3023  CCATGG               C^CATGG
             3236  CCATGG              
NsiI         2139  ATGCAT               ATGCA^T
             3050  ATGCAT              
PaeR7I       4772  CTCGAG               C^TCGAG
             7193  CTCGAG              
PpuMI        4364  GGGACCC              [GA]G^G[AT]CC[CT]
             7583  AGGACCT             
PshAI        1619  GACGAGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             5493  GACTGCTGTC          
PshBI        1807  ATTAAT               AT^TAAT
             5920  ATTAAT              
PstI           10  CTGCAG               CTGCA^G
              377  CTGCAG              
SexAI        5198  ACCAGGT              A^CC[AT]GGT
             6504  ACCAGGT             
StuI         6521  AGGCCT               AGG^CCT
             7349  AGGCCT              
XhoI         4772  CTCGAG               C^TCGAG
             7193  CTCGAG              
XmnI          868  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5028  GAAATGTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  120 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaII       BamHI       BanII       BbsI        BbvI        
BclI        BcnI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
Bsp1407I    BspHI       BspLU11I    BspMI       BsrDI       
BsrGI       BsrI        BssKI       BstEII      BstF5I      
BstNI       BstPI       BstXI       BstYI       Cac8I       
CelII       Cfr13I      DdeI        DpnII       DraI        
DraII       EaeI        EarI        EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoT14I     FbaI        
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MamI        MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PvuII       
RcaI        RsaI        SacI        SapI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SmlI        SphI        SspBI       SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com