Restriction map for pF1KE2538
 (Linear) (Six-base) MAPSORT of: KIEE2538.seq  Check: 3539  from: 1  to: 7692



KIEE2538.orf

 With 182 enzymes: SgfI * 

                        February 10, 2017 14:54  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     594    6162    7692 
   Size:        594    5568    1530

  Fragments arranged by size: 

                5568    1530     594
 
AccI GT'mk_AC

Cuts at:      0     329     548    3094    3684    3933    7692 
   Size:        329     219    2546     590     249    3759

  Fragments arranged by size: 

                3759    2546     590     329     249     219
 
AclI AA'CG_TT

Cuts at:      0    4987    7692 
   Size:       4987    2705
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     213     307     962    1547    2426    2441    2632 
   Size:         89     124      94     655     585     879      15     191

Cuts at:   2632    4063    4246    4344    4846    4955    5320    5371    6123 
   Size:       1431     183      98     502     109     365      51     752

Cuts at:   6123    6967    7526    7582    7692 
   Size:        844     559      56     110

  Fragments arranged by size: 

                1431     879     844     752     655     585     559     502
                 365     191     183     124     110     109      98      94
                  89      56      51      15
 
AflIII A'CryG_T

Cuts at:      0     795    2964    3732    3886    6681    6901    7692 
   Size:        795    2169     768     154    2795     220     791

  Fragments arranged by size: 

                2795    2169     795     791     768     220     154
 
AhdI GACnn_n'nnGTC

Cuts at:      0    4092    7692 
   Size:       4092    3600
 
AleI CACnn'nnGTG

Cuts at:      0    4889    7692 
   Size:       4889    2803
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2073    7692 
   Size:       2073    5619
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1598    1630    7692 
   Size:       1598      32    6062

  Fragments arranged by size: 

                6062    1598      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     967    2041    2587    5602    5866    6429    6694    7455 
   Size:        967    1074     546    3015     264     563     265     761

Cuts at:   7455    7692 
   Size:        237

  Fragments arranged by size: 

                3015    1074     967     761     563     546     265     264
                 237
 
ApaI G_GGCC'C

Cuts at:      0    4340    7692 
   Size:       4340    3352
 
ApoI r'AATT_y

Cuts at:      0     422     872    1018    1702    1710    1915    2517    2536 
   Size:        422     450     146     684       8     205     602      19

Cuts at:   2536    2898    3015    3222    3346    3425    3552    4063    6071 
   Size:        362     117     207     124      79     127     511    2008

Cuts at:   6071    7692 
   Size:       1621

  Fragments arranged by size: 

                2008    1621     684     602     511     450     422     362
                 207     205     146     127     124     117      79      19
                   8
 
AseI AT'TA_AT

Cuts at:      0    1808    5921    7692 
   Size:       1808    4113    1771

  Fragments arranged by size: 

                4113    1808    1771
 
AvaI C'yCGr_G

Cuts at:      0    4772    7193    7692 
   Size:       4772    2421     499

  Fragments arranged by size: 

                4772    2421     499
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2330    2363    3838    3871    7692 
   Size:       2330      33    1475      33    3821

  Fragments arranged by size: 

                3821    2330    1475      33      33
 
BamHI G'GATC_C

Cuts at:      0    3918    4036    6531    7251    7692 
   Size:       3918     118    2495     720     441

  Fragments arranged by size: 

                3918    2495     720     441     118
 
BanI G'GyrC_C

Cuts at:      0     172    6675    7692 
   Size:        172    6503    1017

  Fragments arranged by size: 

                6503    1017     172
 
BanII G_rGCy'C

Cuts at:      0    1063    1312    3764    4340    4530    5885    7692 
   Size:       1063     249    2452     576     190    1355    1807

  Fragments arranged by size: 

                2452    1807    1355    1063     576     249     190
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1405    2647    2931    3035    4561    6169    6973    7192 
   Size:       1405    1242     284     104    1526    1608     804     219

Cuts at:   7192    7514    7692 
   Size:        322     178

  Fragments arranged by size: 

                1608    1526    1405    1242     804     322     284     219
                 178     104
 
BbvCI CC'TCA_GC

Cuts at:      0    7082    7692 
   Size:       7082     610
 
BclI T'GATC_A

Cuts at:      0    1980    5727    6609    7229    7692 
   Size:       1980    3747     882     620     463

  Fragments arranged by size: 

                3747    1980     882     620     463
 
BfrBI ATG'CAT

Cuts at:      0    2141    3052    7692 
   Size:       2141     911    4640

  Fragments arranged by size: 

                4640    2141     911
 
BglII A'GATC_T

Cuts at:      0     706    1210    7692 
   Size:        706     504    6482

  Fragments arranged by size: 

                6482     706     504
 
BlpI GC'TnA_GC

Cuts at:      0    1062    5206    5590    6617    7692 
   Size:       1062    4144     384    1027    1075

  Fragments arranged by size: 

                4144    1075    1062    1027     384
 
Bme1580I G_kGCm'C

Cuts at:      0    4316    4340    7692 
   Size:       4316      24    3352

  Fragments arranged by size: 

                4316    3352      24
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1618    3697    3924    3956    6580    6708    6784    7431 
   Size:       1618    2079     227      32    2624     128      76     647

Cuts at:   7431    7692 
   Size:        261

  Fragments arranged by size: 

                2624    2079    1618     647     261     227     128      76
                  32
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1229    1251    4765    5202    7692 
   Size:       1229      22    3514     437    2490

  Fragments arranged by size: 

                3514    2490    1229     437      22
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293    1826    2699    3181    6738    7371    7609    7692 
   Size:        293    1533     873     482    3557     633     238      83

  Fragments arranged by size: 

                3557    1533     873     633     482     293     238      83
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    7082    7692 
   Size:       7082     610
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1392    2870    3773    3953    4514    4710    6857    7312 
   Size:       1392    1478     903     180     561     196    2147     455

Cuts at:   7312    7692 
   Size:        380

  Fragments arranged by size: 

                2147    1478    1392     903     561     455     380     196
                 180
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1436    1486    1844    5188    5486    6182    7692 
   Size:       1436      50     358    3344     298     696    1510

  Fragments arranged by size: 

                3344    1510    1436     696     358     298      50
 
BsaAI yAC'GTr

Cuts at:      0     798    3809    3889    7692 
   Size:        798    3011      80    3803

  Fragments arranged by size: 

                3803    3011     798      80
 
BsaBI GATnn'nnATC

Cuts at:      0    1182    2609    5726    7692 
   Size:       1182    1427    3117    1966

  Fragments arranged by size: 

                3117    1966    1427    1182
 
BsaWI w'CCGG_w

Cuts at:      0    3420    7466    7692 
   Size:       3420    4046     226

  Fragments arranged by size: 

                4046    3420     226
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2616    2646    2951    2981    5598    5628    5768    5798 
   Size:       2616      30     305      30    2617      30     140      30

Cuts at:   5798    5944    5974    6736    6766    7019    7049    7549    7579 
   Size:        146      30     762      30     253      30     500      30

Cuts at:   7579    7692 
   Size:        113

  Fragments arranged by size: 

                2617    2616     762     500     305     253     146     140
                 113      30      30      30      30      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2966    4027    5084    5965    7136    7692 
   Size:       2966    1061    1057     881    1171     556

  Fragments arranged by size: 

                2966    1171    1061    1057     881     556
 
BseYI C'CCAG_C

Cuts at:      0    1834    4682    6535    6574    6971    7692 
   Size:       1834    2848    1853      39     397     721

  Fragments arranged by size: 

                2848    1853    1834     721     397      39
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     254    6848    7692 
   Size:        124     130    6594     844

  Fragments arranged by size: 

                6594     844     130     124
 
BsiEI CG_ry'CG

Cuts at:      0    5367    7692 
   Size:       5367    2325
 
BsiHKAI G_wGCw'C

Cuts at:      0      59    1063    1312    1365    3764    4530    5207    5553 
   Size:         59    1004     249      53    2399     766     677     346

Cuts at:   5553    5596    6563    7692 
   Size:         43     967    1129

  Fragments arranged by size: 

                2399    1129    1004     967     766     677     346     249
                  59      53      43
 
BsmI GAATG_Cn'

Cuts at:      0    4493    5809    7692 
   Size:       4493    1316    1883

  Fragments arranged by size: 

                4493    1883    1316
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3199    7692 
   Size:       3199    4493
 
Bsp1286I G_dGCh'C

Cuts at:      0      59    1063    1312    1365    3764    4316    4340    4530 
   Size:         59    1004     249      53    2399     552      24     190

Cuts at:   4530    5207    5553    5596    5885    6563    7692 
   Size:        677     346      43     289     678    1129

  Fragments arranged by size: 

                2399    1129    1004     678     677     552     346     289
                 249     190      59      53      43      24
 
BspEI T'CCGG_A

Cuts at:      0    3420    7692 
   Size:       3420    4272
 
BspHI T'CATG_A

Cuts at:      0    3102    4574    4877    7692 
   Size:       3102    1472     303    2815

  Fragments arranged by size: 

                3102    2815    1472     303
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     594    2212    6162    7692 
   Size:        594    1618    3950    1530

  Fragments arranged by size: 

                3950    1618    1530     594
 
BsrBI CCG'CTC

Cuts at:      0    6267    7692 
   Size:       6267    1425
 
BsrDI GCAATG_nn'

Cuts at:      0     285    3912    7602    7692 
   Size:        285    3627    3690      90

  Fragments arranged by size: 

                3690    3627     285      90
 
BsrFI r'CCGG_y

Cuts at:      0    6777    7692 
   Size:       6777     915
 
BsrGI T'GTAC_A

Cuts at:      0    1723    4501    6843    6957    7692 
   Size:       1723    2778    2342     114     735

  Fragments arranged by size: 

                2778    2342    1723     735     114
 
BssSI C'ACGA_G

Cuts at:      0    1364    7692 
   Size:       1364    6328
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    6420    7692 
   Size:       6420    1272
 
BstBI TT'CG_AA

Cuts at:      0    4714    7692 
   Size:       4714    2978
 
BstEII G'GTnAC_C

Cuts at:      0    3544    5059    6496    7692 
   Size:       3544    1515    1437    1196

  Fragments arranged by size: 

                3544    1515    1437    1196
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    4376    4962    5011    6036    6733    7692 
   Size:       4376     586      49    1025     697     959

  Fragments arranged by size: 

                4376    1025     959     697     586      49
 
BstYI r'GATC_y

Cuts at:      0     706    1083    1210    2023    3918    4036    5339    5721 
   Size:        706     377     127     813    1895     118    1303     382

Cuts at:   5721    6531    7128    7197    7251    7692 
   Size:        810     597      69      54     441

  Fragments arranged by size: 

                1895    1303     813     810     706     597     441     382
                 377     127     118      69      54
 
Bsu36I CC'TnA_GG

Cuts at:      0    1447    7580    7692 
   Size:       1447    6133     112

  Fragments arranged by size: 

                6133    1447     112
 
BtgI C'CryG_G

Cuts at:      0    3023    3236    7692 
   Size:       3023     213    4456

  Fragments arranged by size: 

                4456    3023     213
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    4389    5528    7692 
   Size:       4389    1139    2164

  Fragments arranged by size: 

                4389    2164    1139
 
BtsI GCAGTG_nn'

Cuts at:      0     266     463     530    2735    4498    6445    7058    7692 
   Size:        266     197      67    2205    1763    1947     613     634

  Fragments arranged by size: 

                2205    1947    1763     634     613     266     197      67
            
 
ClaI AT'CG_AT

Cuts at:      0    1857    7692 
   Size:       1857    5835
 
DraI TTT'AAA

Cuts at:      0    1016    1053    1073    1707    1880    2762    4767    7515 
   Size:       1016      37      20     634     173     882    2005    2748

Cuts at:   7515    7692 
   Size:        177

  Fragments arranged by size: 

                2748    2005    1016     882     634     177     173      37
                  20
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    6902    7692 
   Size:       6902     790
 
EaeI y'GGCC_r

Cuts at:      0    1342    5364    6023    7692 
   Size:       1342    4022     659    1669

  Fragments arranged by size: 

                4022    1669    1342     659
 
EagI C'GGCC_G

Cuts at:      0    5364    7692 
   Size:       5364    2328
 
EarI CTCTTCn'nnn_

Cuts at:      0     326    1382    1437    1512    2651    2913    3126    3593 
   Size:        326    1056      55      75    1139     262     213     467

Cuts at:   3593    3752    4004    5387    5750    5942    6258    7692 
   Size:        159     252    1383     363     192     316    1434

  Fragments arranged by size: 

                1434    1383    1139    1056     467     363     326     316
                 262     252     213     192     159      75      55
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     557    1218    7692 
   Size:        557     661    6474

  Fragments arranged by size: 

                6474     661     557
 
EcoICRI GAG'CTC

Cuts at:      0    1061    1310    3762    4528    7692 
   Size:       1061     249    2452     766    3164

  Fragments arranged by size: 

                3164    2452    1061     766     249
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     213     293     307     962    1547    1826    2426 
   Size:         89     124      80      14     655     585     279     600

Cuts at:   2426    2441    2632    2699    3181    4063    4246    4344    4846 
   Size:         15     191      67     482     882     183      98     502

Cuts at:   4846    4955    5320    5371    6123    6738    6967    7371    7526 
   Size:        109     365      51     752     615     229     404     155

Cuts at:   7526    7582    7609    7692 
   Size:         56      27      83

  Fragments arranged by size: 

                 882     752     655     615     600     585     502     482
                 404     365     279     229     191     183     155     124
                 109      98      89      83      80      67      56      51
                  27      15      14
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1257    2813    6058    7692 
   Size:       1257    1556    3245    1634

  Fragments arranged by size: 

                3245    1634    1556    1257
 
EcoO109I rG'GnC_Cy

Cuts at:      0    4204    4337    4365    7584    7692 
   Size:       4204     133      28    3219     108

  Fragments arranged by size: 

                4204    3219     133     108      28
 
EcoRI G'AATT_C

Cuts at:      0    2898    3222    6071    7692 
   Size:       2898     324    2849    1621

  Fragments arranged by size: 

                2898    2849    1621     324
 
EcoRV GAT'ATC

Cuts at:      0    4191    7692 
   Size:       4191    3501
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    4576    5740    7692 
   Size:       4576    1164    1952

  Fragments arranged by size: 

                4576    1952    1164
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     355     387     760     792     817     849    1948    1980 
   Size:        355      32     373      32      25      32    1099      32

Cuts at:   1980    2695    2727    2918    2950    3514    3546    3910    3942 
   Size:        715      32     191      32     564      32     364      32

Cuts at:   3942    4078    4110    5072    5104    5527    5559    5944    5976 
   Size:        136      32     962      32     423      32     385      32

Cuts at:   5976    7104    7136    7692 
   Size:       1128      32     556

  Fragments arranged by size: 

                1128    1099     962     715     564     556     423     385
                 373     364     355     191     136      32      32      32
                  32      32      32      32      32      32      32      32
                  32      32      25
 
HincII GTy'rAC

Cuts at:      0    4985    7692 
   Size:       4985    2707
 
HindIII A'AGCT_T

Cuts at:      0    5383    7692 
   Size:       5383    2309
 
HpaI GTT'AAC

Cuts at:      0    4985    7692 
   Size:       4985    2707
 
MfeI C'AATT_G

Cuts at:      0    5049    6953    7692 
   Size:       5049    1904     739

  Fragments arranged by size: 

                5049    1904     739
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1641    5423    6025    6931    7348    7692 
   Size:       1641    3782     602     906     417     344

  Fragments arranged by size: 

                3782    1641     906     602     417     344
 
MscI TGG'CCA

Cuts at:      0    6025    7692 
   Size:       6025    1667
 
MslI CAynn'nnrTG

Cuts at:      0    1280    2284    2600    3057    3335    3521    3737    4374 
   Size:       1280    1004     316     457     278     186     216     637

Cuts at:   4374    4649    4889    7692 
   Size:        275     240    2803

  Fragments arranged by size: 

                2803    1280    1004     637     457     316     278     275
                 240     216     186
 
MspA1I CmG'CkG

Cuts at:      0       9     313    4889    5796    7692 
   Size:          9     304    4576     907    1896

  Fragments arranged by size: 

                4576    1896     907     304       9
 
NaeI GCC'GGC

Cuts at:      0    6779    7692 
   Size:       6779     913
 
NcoI C'CATG_G

Cuts at:      0    3023    3236    7692 
   Size:       3023     213    4456

  Fragments arranged by size: 

                4456    3023     213
 
NdeI CA'TA_TG

Cuts at:      0    7329    7692 
   Size:       7329     363
 
NgoMIV G'CCGG_C

Cuts at:      0    6777    7692 
   Size:       6777     915
 
NsiI A_TGCA'T

Cuts at:      0    2143    3054    7692 
   Size:       2143     911    4638

  Fragments arranged by size: 

                4638    2143     911
 
NspI r_CATG'y

Cuts at:      0    2141    2145    2211    2546    2770    2968    3736    4547 
   Size:       2141       4      66     335     224     198     768     811

Cuts at:   4547    4648    6685    6905    7644    7692 
   Size:        101    2037     220     739      48

  Fragments arranged by size: 

                2141    2037     811     768     739     335     224     220
                 198     101      66      48       4
 
PciI A'CATG_T

Cuts at:      0    2964    3732    6681    6901    7692 
   Size:       2964     768    2949     220     791

  Fragments arranged by size: 

                2964    2949     791     768     220
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    6720    7692 
   Size:       6720     972
 
PfoI T'CCnGG_A

Cuts at:      0    3939    6035    7692 
   Size:       3939    2096    1657

  Fragments arranged by size: 

                3939    2096    1657
 
PmlI CAC'GTG

Cuts at:      0    3889    7692 
   Size:       3889    3803
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    5269    5301    7692 
   Size:       5269      32    2391

  Fragments arranged by size: 

                5269    2391      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    4365    7584    7692 
   Size:       4365    3219     108

  Fragments arranged by size: 

                4365    3219     108
 
PshAI GACnn'nnGTC

Cuts at:      0    1623    5497    7692 
   Size:       1623    3874    2195

  Fragments arranged by size: 

                3874    2195    1623
 
PsiI TTA'TAA

Cuts at:      0     730    1664    1921    5401    5650    6470    7692 
   Size:        730     934     257    3480     249     820    1222

  Fragments arranged by size: 

                3480    1222     934     820     730     257     249
 
PspOMI G'GGCC_C

Cuts at:      0    4336    7692 
   Size:       4336    3356
 
PspXI vC'TCGA_Gb

Cuts at:      0    4772    7692 
   Size:       4772    2920
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2562    2594    2733    2765    7692 
   Size:       2562      32     139      32    4927

  Fragments arranged by size: 

                4927    2562     139      32      32
 
PstI C_TGCA'G

Cuts at:      0      14     381    7692 
   Size:         14     367    7311

  Fragments arranged by size: 

                7311     367      14
 
PvuII CAG'CTG

Cuts at:      0       9     313    4889    5796    7692 
   Size:          9     304    4576     907    1896

  Fragments arranged by size: 

                4576    1896     907     304       9
 
SacI G_AGCT'C

Cuts at:      0    1063    1312    3764    4530    7692 
   Size:       1063     249    2452     766    3162

  Fragments arranged by size: 

                3162    2452    1063     766     249
 
SanDI GG'GwC_CC

Cuts at:      0    4365    7692 
   Size:       4365    3327
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3126    3593    3752    7692 
   Size:       3126     467     159    3940

  Fragments arranged by size: 

                3940    3126     467     159
 
ScaI AGT'ACT

Cuts at:      0    5459    6856    7567    7692 
   Size:       5459    1397     711     125

  Fragments arranged by size: 

                5459    1397     711     125
 
SexAI A'CCwGG_T

Cuts at:      0    5198    6504    7692 
   Size:       5198    1306    1188

  Fragments arranged by size: 

                5198    1306    1188
 
SfcI C'TryA_G

Cuts at:      0      10     377     545    3095    5747    6350    6638    7692 
   Size:         10     367     168    2550    2652     603     288    1054

  Fragments arranged by size: 

                2652    2550    1054     603     367     288     168      10
            
 
SmlI C'TyrA_G

Cuts at:      0    1407    2885    3788    3968    4529    4689    4772    6872 
   Size:       1407    1478     903     180     561     160      83    2100

Cuts at:   6872    7193    7291    7692 
   Size:        321      98     401

  Fragments arranged by size: 

                2100    1478    1407     903     561     401     321     180
                 160      98      83
 
SpeI A'CTAG_T

Cuts at:      0    2575    7692 
   Size:       2575    5117
 
SphI G_CATG'C

Cuts at:      0    2141    2145    2211    2770    4648    7692 
   Size:       2141       4      66     559    1878    3044

  Fragments arranged by size: 

                3044    2141    1878     559      66       4
 
SspI AAT'ATT

Cuts at:      0     809    1129    2169    2433    4735    7692 
   Size:        809     320    1040     264    2302    2957

  Fragments arranged by size: 

                2957    2302    1040     809     320     264
 
StuI AGG'CCT

Cuts at:      0    6523    7351    7692 
   Size:       6523     828     341

  Fragments arranged by size: 

                6523     828     341
 
StyI C'CwwG_G

Cuts at:      0    3023    3236    6623    7619    7692 
   Size:       3023     213    3387     996      73

  Fragments arranged by size: 

                3387    3023     996     213      73
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3563    6588    7692 
   Size:       3563    3025    1104

  Fragments arranged by size: 

                3563    3025    1104
 
TatI w'GTAC_w

Cuts at:      0    1723    2383    3508    4501    5457    6741    6843    6854 
   Size:       1723     660    1125     993     956    1284     102      11

Cuts at:   6854    6957    7565    7625    7637    7692 
   Size:        103     608      60      12      55

  Fragments arranged by size: 

                1723    1284    1125     993     956     660     608     103
                 102      60      55      12      11
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2657    2689    2951    2983    4371    4403    5275    5307 
   Size:       2657      32     262      32    1388      32     872      32

Cuts at:   5307    5559    5591    5598    5630    6574    6606    6736    6768 
   Size:        252      32       7      32     944      32     130      32

Cuts at:   6768    7002    7034    7576    7608    7692 
   Size:        234      32     542      32      84

  Fragments arranged by size: 

                2657    1388     944     872     542     262     252     234
                 130      84      32      32      32      32      32      32
                  32      32      32      32       7
 
XbaI T'CTAG_A

Cuts at:      0    1237    7692 
   Size:       1237    6455
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     465    3644    6033    6576    7692 
   Size:        465    3179    2389     543    1116

  Fragments arranged by size: 

                3179    2389    1116     543     465
 
XhoI C'TCGA_G

Cuts at:      0    4772    7193    7692 
   Size:       4772    2421     499

  Fragments arranged by size: 

                4772    2421     499
 
XmnI GAAnn'nnTTC

Cuts at:      0     872    5032    7692 
   Size:        872    4160    2660

  Fragments arranged by size: 

                4160    2660     872
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI   AflIII     AhdI     AleI     AlfI
     AloI    AlwNI     ApaI     ApoI     AseI     AvaI     BaeI    BamHI
     BanI    BanII     BbsI    BbvCI     BclI    BfrBI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI    Hin4I   HincII  HindIII     HpaI     MfeI     MmeI
     MscI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NsiI
     NspI     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI    PvuII     SacI    SanDI
     SapI     ScaI    SexAI     SfcI     SmlI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI     XbaI     XcmI     XhoI
     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AfeI    AflII     AgeI    ApaLI     AscI    AsiSI
    AvrII     BbeI     BcgI    BciVI     BglI    BmgBI     BmtI    BsaHI
    BsiWI   BssHII  BstZ17I    CspCI   DraIII     FseI     FspI    FspAI
    HaeII     KasI     KpnI     MluI     NarI     NheI     NotI     NruI
     PacI     PasI     PmeI     PvuI    RsrII    SacII     SalI     SbfI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SrfI     SwaI
  Tth111I     XmaI     ZraI
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