Restriction map for pF1KE1399
##################################
# NO CUT ENZYMES (   51 enzymes) #
##################################

Acc65I      AgeI        AhdI        AscI        AseI        
AsnI        Asp718I     AspEI       AviII       AvrII       
BbvCI       BciVI       BclI        BlnI        BsiWI       
BspLU11I    BssHII      Bst1107I    BstBI       BstZ17I     
DraI        EagI        Eam1105I    EclXI       Eco52I      
FbaI        FseI        FspI        KpnI        MfeI        
MluI        MunI        NdeI        NheI        NotI        
NruI        NspV        PacI        PinAI       PmeI        
PshBI       PvuI        SalI        ScaI        SfuI        
SnaBI       SpeI        SphI        SspI        SwaI        
XbaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AatII        4819  GACGTC               GACGT^C
AccIII       2294  TCCGGA               T^CCGGA
AclI         4864  AACGTT               AA^CGTT
AflII        2140  CTTAAG               C^TTAAG
AflIII       3911  ACACGT               A^C[GA][CT]GT
Alw44I       3946  GTGCAC               G^TGCAC
ApaLI        3946  GTGCAC               G^TGCAC
Asp700I      1800  GAAGCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI         3478  GGCGCC               GGCGC^C
BbrPI         928  CACGTG               CAC^GTG
BfrI         2140  CTTAAG               C^TTAAG
BglII        4079  AGATCT               A^GATCT
BsaBI        3897  GATCGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        2294  TCCGGA               T^CCGGA
BsiEI        3261  CGACCG               CG[GA][CT]^CG
Bsp1407I      265  TGTACA               T^GTACA
BspEI        2294  TCCGGA               T^CCGGA
BspHI        4991  TCATGA               T^CATGA
BsrGI         265  TGTACA               T^GTACA
CpoI         2291  CGGTCCG              CG^G[AT]CCG
DrdI         1294  GACTGGGCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV        4264  GATATC               GAT^ATC
HpaI         2062  GTTAAC               GTT^AAC
KasI         3478  GGCGCC               G^GCGCC
MamI         3897  GATCGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2294  TCCGGA               T^CCGGA
NarI         3478  GGCGCC               GG^CGCC
NcoI         4139  CCATGG               C^CATGG
PmaCI         928  CACGTG               CAC^GTG
PmlI          928  CACGTG               CAC^GTG
PshAI        1875  GACGGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     4864  AACGTT               AA^CGTT
RcaI         4991  TCATGA               T^CATGA
RsrII        2291  CGGTCCG              CG^G[AT]CCG
SfiI         3711  GGCCCCCAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         3478  GGCGCC               GGC^GCC
SgrAI        1207  CGCCGGCG             C[GA]^CCGG[CT]G
SspBI         265  TGTACA               T^GTACA
XmnI         1800  GAAGCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AccI         2746  GTCTAC               GT^[AC][GT]AC
             2998  GTCTAC              
AocI         3816  CCTGAGG              CC^T[ACGT]AGG
             5102  CCTCAGG             
Aor51HI      1285  AGCGCT               AGC^GCT
             3409  AGCGCT              
AspI         1651  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4450  GACGCCGTC           
BamHI         257  GGATCC               G^GATCC
              561  GGATCC              
BfaI         3488  CTAG                 C^TAG
             4541  CTAG                
BlpI          613  GCTCAGC              GC^T[ACGT]AGC
             3412  GCTGAGC             
Bpu1102I      613  GCTCAGC              GC^T[ACGT]AGC
             3412  GCTGAGC             
BsaAI         928  CACGTG               [CT]AC^GT[GA]
             1399  TACGTG              
BsmBI        3829  GAGACG               CGTCTC(1/5)
             4513  GAGACG              
BspDI        5434  ATCGAT               AT^CGAT
             5476  ATCGAT              
Bsu36I       3816  CCTGAGG              CC^T[ACGT]AGG
             5102  CCTCAGG             
CelII         613  GCTCAGC              GC^T[ACGT]AGC
             3412  GCTGAGC             
ClaI         5434  ATCGAT               AT^CGAT
             5476  ATCGAT              
Eco47III     1285  AGCGCT               AGC^GCT
             3409  AGCGCT              
Eco81I       3816  CCTGAGG              CC^T[ACGT]AGG
             5102  CCTCAGG             
EcoNI         345  CCTGGATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             5457  CCTGCAGAAGG         
EcoT22I       210  ATGCAT               ATGCA^T
              498  ATGCAT              
HincII       2062  GTTAAC               GT[CT]^[GA]AC
             4621  GTTGAC              
HindII       2062  GTTAAC               GT[CT]^[GA]AC
             4621  GTTGAC              
HindIII      1801  AAGCTT               A^AGCTT
             4443  AAGCTT              
KspI         1644  CCGCGG               CCGC^GG
             1704  CCGCGG              
MaeI         3488  CTAG                 C^TAG
             4541  CTAG                
NsiI          210  ATGCAT               ATGCA^T
              498  ATGCAT              
NspI         1485  GCATGT               [GA]CATG^[CT]
             3890  ACATGC              
PflFI        1651  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4450  GACGCCGTC           
SacI          662  GAGCTC               GAGCT^C
             2272  GAGCTC              
SacII        1644  CCGCGG               CCGC^GG
             1704  CCGCGG              
SapI         2584  GAAGAGC              GCTCTTC(1/4)
             1587  GCTCTTC             
SbfI          914  CCTGCAGG             CCTGCA^GG
             3981  CCTGCAGG            
SexAI        2132  ACCTGGT              A^CC[AT]GGT
             2806  ACCAGGT             
Sse8387I      914  CCTGCAGG             CCTGCA^GG
             3981  CCTGCAGG            
Tth111I      1651  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4450  GACGCCGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  135 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        ApoI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvI        BcgI        BcnI        
BglI        BmyI        BpmI        BpuAI       BsaHI       
BsaI        BsaJI       BsaWI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    BspMI       BsrBI       
BsrDI       BsrFI       BsrI        BssKI       BssSI       
BstAPI      BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DraIII      DsaI        EaeI        EarI        EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
PaeR7I      PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        SmlI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoI        XhoII       XmaCI       XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com