(Linear) (Six-base) MAPSORT of: KIEE3547.seq Check: 1835 from: 1 to: 6189 KIEE3547.orf With 182 enzymes: SgfI * July 9, 2014 09:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1519 1651 3726 6189 Size: 1519 132 2075 2463 Fragments arranged by size: 2463 2075 1519 132 AatII G_ACGT'C Cuts at: 0 3017 4763 5696 6189 Size: 3017 1746 933 493 Fragments arranged by size: 3017 1746 933 493 AccI GT'mk_AC Cuts at: 0 1368 5195 5384 5690 6189 Size: 1368 3827 189 306 499 Fragments arranged by size: 3827 1368 499 306 189 Acc65I G'GTAC_C Cuts at: 0 1987 6189 Size: 1987 4202 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1711 2126 2261 3307 3691 3979 4474 6189 Size: 1711 415 135 1046 384 288 495 1715 Fragments arranged by size: 1715 1711 1046 495 415 384 288 135 AfeI AGC'GCT Cuts at: 0 61 5777 6189 Size: 61 5716 412 Fragments arranged by size: 5716 412 61 AflIII A'CryG_T Cuts at: 0 2865 5252 5552 5645 6189 Size: 2865 2387 300 93 544 Fragments arranged by size: 2865 2387 544 300 93 AgeI A'CCGG_T Cuts at: 0 3914 6189 Size: 3914 2275 AhdI GACnn_n'nnGTC Cuts at: 0 1212 2131 2342 2736 4923 6146 6189 Size: 1212 919 211 394 2187 1223 43 Fragments arranged by size: 2187 1223 1212 919 394 211 43 AleI CACnn'nnGTG Cuts at: 0 3047 6189 Size: 3047 3142 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1992 3280 4504 5771 6189 Size: 1992 1288 1224 1267 418 Fragments arranged by size: 1992 1288 1267 1224 418 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 3028 3060 6189 Size: 3028 32 3129 Fragments arranged by size: 3129 3028 32 AlwNI CAG_nnn'CTG Cuts at: 0 1726 1915 1954 2149 2354 3285 3603 4209 Size: 1726 189 39 195 205 931 318 606 Cuts at: 4209 4272 6189 Size: 63 1917 Fragments arranged by size: 1917 1726 931 606 318 205 195 189 63 39 ApaI G_GGCC'C Cuts at: 0 194 352 681 772 2079 2533 2664 2850 Size: 194 158 329 91 1307 454 131 186 Cuts at: 2850 6189 Size: 3339 Fragments arranged by size: 3339 1307 454 329 194 186 158 131 91 ApaLI G'TGCA_C Cuts at: 0 5707 6189 Size: 5707 482 AvaI C'yCGr_G Cuts at: 0 76 149 302 485 680 822 877 1880 Size: 76 73 153 183 195 142 55 1003 Cuts at: 1880 2742 2843 2975 3007 3352 4327 4669 4842 Size: 862 101 132 32 345 975 342 173 Cuts at: 4842 4941 5007 5165 5998 6189 Size: 99 66 158 833 191 Fragments arranged by size: 1003 975 862 833 345 342 195 191 183 173 158 153 142 132 101 99 76 73 66 55 32 BamHI G'GATC_C Cuts at: 0 1740 3635 6189 Size: 1740 1895 2554 Fragments arranged by size: 2554 1895 1740 BanI G'GyrC_C Cuts at: 0 12 463 1321 1729 1816 1987 2110 2422 Size: 12 451 858 408 87 171 123 312 Cuts at: 2422 2629 2910 2997 4333 4665 4731 4896 5110 Size: 207 281 87 1336 332 66 165 214 Cuts at: 5110 5443 5809 5871 6038 6189 Size: 333 366 62 167 151 Fragments arranged by size: 1336 858 451 408 366 333 332 312 281 214 207 171 167 165 151 123 87 87 66 62 12 BanII G_rGCy'C Cuts at: 0 194 352 681 772 792 884 979 986 Size: 194 158 329 91 20 92 95 7 Cuts at: 986 1005 1280 1480 1712 1907 2079 2284 2293 Size: 19 275 200 232 195 172 205 9 Cuts at: 2293 2306 2533 2546 2564 2591 2664 2850 2982 Size: 13 227 13 18 27 73 186 132 Cuts at: 2982 3359 3438 4535 4742 5136 5218 5458 5530 Size: 377 79 1097 207 394 82 240 72 Cuts at: 5530 6189 Size: 659 Fragments arranged by size: 1097 659 394 377 329 275 240 232 227 207 205 200 195 194 186 172 158 132 95 92 91 82 79 73 72 27 20 19 18 13 13 9 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 4073 4919 6189 Size: 4073 846 1270 Fragments arranged by size: 4073 1270 846 BbvCI CC'TCA_GC Cuts at: 0 82 1453 2216 2316 3113 4711 6189 Size: 82 1371 763 100 797 1598 1478 Fragments arranged by size: 1598 1478 1371 797 763 100 82 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 378 412 2434 2468 4753 4787 6189 Size: 378 34 2022 34 2285 34 1402 Fragments arranged by size: 2285 2022 1402 378 34 34 34 BclI T'GATC_A Cuts at: 0 3617 6189 Size: 3617 2572 BglI GCCn_nnn'nGGC Cuts at: 0 165 1154 3538 3778 4746 6189 Size: 165 989 2384 240 968 1443 Fragments arranged by size: 2384 1443 989 968 240 165 BglII A'GATC_T Cuts at: 0 3335 6189 Size: 3335 2854 BlpI GC'TnA_GC Cuts at: 0 3743 4591 6189 Size: 3743 848 1598 Fragments arranged by size: 3743 1598 848 Bme1580I G_kGCm'C Cuts at: 0 15 194 352 681 772 1821 2079 2115 Size: 15 179 158 329 91 1049 258 36 Cuts at: 2115 2533 2649 2664 2850 2888 4336 4670 4901 Size: 418 116 15 186 38 1448 334 231 Cuts at: 4901 5233 5446 5643 5711 5812 5874 6189 Size: 332 213 197 68 101 62 315 Fragments arranged by size: 1448 1049 418 334 332 329 315 258 231 213 197 186 179 158 116 101 91 68 62 38 36 15 15 BmgBI CAC'GTC Cuts at: 0 4614 5916 6189 Size: 4614 1302 273 Fragments arranged by size: 4614 1302 273 BmrI ACTGGGnnnn_n' Cuts at: 0 1872 2261 3058 6189 Size: 1872 389 797 3131 Fragments arranged by size: 3131 1872 797 389 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1923 3457 4736 6189 Size: 1923 1534 1279 1453 Fragments arranged by size: 1923 1534 1453 1279 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 76 84 396 732 806 1337 2555 2844 Size: 76 8 312 336 74 531 1218 289 Cuts at: 2844 3382 3403 3733 3826 4327 4706 4968 5130 Size: 538 21 330 93 501 379 262 162 Cuts at: 5130 5287 5566 5589 6037 6189 Size: 157 279 23 448 152 Fragments arranged by size: 1218 538 531 501 448 379 336 330 312 289 279 262 162 157 152 93 76 74 23 21 8 Bpu10I CC'TnA_GC Cuts at: 0 82 883 980 1453 1955 2216 2316 2915 Size: 82 801 97 473 502 261 100 599 Cuts at: 2915 3113 4711 5524 6189 Size: 198 1598 813 665 Fragments arranged by size: 1598 813 801 665 599 502 473 261 198 100 97 82 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 132 1180 1585 3541 5197 6189 Size: 132 1048 405 1956 1656 992 Fragments arranged by size: 1956 1656 1048 992 405 132 BsaI GGTCTCn'nnnn_ Cuts at: 0 568 1183 1402 2086 3923 4996 5026 6189 Size: 568 615 219 684 1837 1073 30 1163 Fragments arranged by size: 1837 1163 1073 684 615 568 219 30 BsaAI yAC'GTr Cuts at: 0 3207 4572 5253 5562 5646 6189 Size: 3207 1365 681 309 84 543 Fragments arranged by size: 3207 1365 681 543 309 84 BsaHI Gr'CG_yC Cuts at: 0 184 314 508 3014 4370 4760 4858 5693 Size: 184 130 194 2506 1356 390 98 835 Cuts at: 5693 6189 Size: 496 Fragments arranged by size: 2506 1356 835 496 390 194 184 130 98 BsaWI w'CCGG_w Cuts at: 0 3046 3914 4734 6189 Size: 3046 868 820 1455 Fragments arranged by size: 3046 1455 868 820 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 274 304 3028 3058 5200 5230 5248 5278 Size: 274 30 2724 30 2142 30 18 30 Cuts at: 5278 5363 5393 5539 5569 5785 5815 6013 6043 Size: 85 30 146 30 216 30 198 30 Cuts at: 6043 6189 Size: 146 Fragments arranged by size: 2724 2142 274 216 198 146 146 85 30 30 30 30 30 30 30 30 18 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 103 374 684 687 690 769 778 810 Size: 103 271 310 3 3 79 9 32 Cuts at: 810 1115 1692 1750 1915 2847 3790 4691 4728 Size: 305 577 58 165 932 943 901 37 Cuts at: 4728 4971 5014 5062 5065 5690 5806 6163 6189 Size: 243 43 48 3 625 116 357 26 Fragments arranged by size: 943 932 901 625 577 357 310 305 271 243 165 116 103 79 58 48 43 37 32 26 9 3 3 3 BseYI C'CCAG_C Cuts at: 0 23 295 351 370 421 451 863 1235 Size: 23 272 56 19 51 30 412 372 Cuts at: 1235 1267 1566 1572 2491 2512 2775 3419 4933 Size: 32 299 6 919 21 263 644 1514 Cuts at: 4933 5226 6189 Size: 293 963 Fragments arranged by size: 1514 963 919 644 412 372 299 293 272 263 56 51 32 30 23 21 19 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1795 3025 3083 3649 3933 6189 Size: 1795 1230 58 566 284 2256 Fragments arranged by size: 2256 1795 1230 566 284 58 BsiEI CG_ry'CG Cuts at: 0 136 329 344 598 840 3899 6189 Size: 136 193 15 254 242 3059 2290 Fragments arranged by size: 3059 2290 254 242 193 136 15 BsiHKAI G_wGCw'C Cuts at: 0 1480 1689 1712 1907 3311 3359 3368 5218 Size: 1480 209 23 195 1404 48 9 1850 Cuts at: 5218 5552 5711 6023 6189 Size: 334 159 312 166 Fragments arranged by size: 1850 1480 1404 334 312 209 195 166 159 48 23 9 BsmBI CGTCTCn'nnnn_ Cuts at: 0 45 299 836 2801 4720 6146 6189 Size: 45 254 537 1965 1919 1426 43 Fragments arranged by size: 1965 1919 1426 537 254 45 43 Bsp1286I G_dGCh'C Cuts at: 0 15 194 352 681 772 792 884 979 Size: 15 179 158 329 91 20 92 95 Cuts at: 979 986 1005 1280 1480 1689 1712 1821 1907 Size: 7 19 275 200 209 23 109 86 Cuts at: 1907 2079 2115 2284 2293 2306 2533 2546 2564 Size: 172 36 169 9 13 227 13 18 Cuts at: 2564 2591 2649 2664 2850 2888 2982 3311 3359 Size: 27 58 15 186 38 94 329 48 Cuts at: 3359 3368 3438 4336 4535 4670 4742 4901 5136 Size: 9 70 898 199 135 72 159 235 Cuts at: 5136 5218 5233 5446 5458 5530 5552 5643 5711 Size: 82 15 213 12 72 22 91 68 Cuts at: 5711 5812 5874 6023 6189 Size: 101 62 149 166 Fragments arranged by size: 898 329 329 275 235 227 213 209 200 199 186 179 172 169 166 159 158 149 135 109 101 95 94 92 91 91 86 82 72 72 70 68 62 58 48 38 36 27 23 22 20 19 18 15 15 15 13 13 12 9 9 7 BspHI T'CATG_A Cuts at: 0 3614 4469 6189 Size: 3614 855 1720 Fragments arranged by size: 3614 1720 855 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1519 1651 3047 3378 3726 5192 5332 5966 Size: 1519 132 1396 331 348 1466 140 634 Cuts at: 5966 6189 Size: 223 Fragments arranged by size: 1519 1466 1396 634 348 331 223 140 132 BsrBI CCG'CTC Cuts at: 0 68 222 615 1250 5144 6189 Size: 68 154 393 635 3894 1045 Fragments arranged by size: 3894 1045 635 393 154 68 BsrDI GCAATG_nn' Cuts at: 0 2175 4832 5001 6189 Size: 2175 2657 169 1188 Fragments arranged by size: 2657 2175 1188 169 BsrFI r'CCGG_y Cuts at: 0 131 593 3914 4967 5908 6189 Size: 131 462 3321 1053 941 281 Fragments arranged by size: 3321 1053 941 462 281 131 BsrGI T'GTAC_A Cuts at: 0 4001 6189 Size: 4001 2188 BssHII G'CGCG_C Cuts at: 0 4599 6189 Size: 4599 1590 BssSI C'ACGA_G Cuts at: 0 126 3370 6189 Size: 126 3244 2819 Fragments arranged by size: 3244 2819 126 BstAPI GCAn_nnn'nTGC Cuts at: 0 3030 3173 3402 3517 3576 4652 5715 6189 Size: 3030 143 229 115 59 1076 1063 474 Fragments arranged by size: 3030 1076 1063 474 229 143 115 59 BstEII G'GTnAC_C Cuts at: 0 4876 6189 Size: 4876 1313 BstXI CCAn_nnnn'nTGG Cuts at: 0 2047 5121 6189 Size: 2047 3074 1068 Fragments arranged by size: 3074 2047 1068 BstYI r'GATC_y Cuts at: 0 1740 1802 3335 3347 3635 3837 6189 Size: 1740 62 1533 12 288 202 2352 Fragments arranged by size: 2352 1740 1533 288 202 62 12 Bsu36I CC'TnA_GG Cuts at: 0 2669 3128 3683 4541 5201 6189 Size: 2669 459 555 858 660 988 Fragments arranged by size: 2669 988 858 660 555 459 BtgI C'CryG_G Cuts at: 0 140 323 329 807 1161 1488 1658 2040 Size: 140 183 6 478 354 327 170 382 Cuts at: 2040 2321 2499 5024 5151 5866 6189 Size: 281 178 2525 127 715 323 Fragments arranged by size: 2525 715 478 382 354 327 323 281 183 178 170 140 127 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 690 1853 2987 4611 6189 Size: 690 1163 1134 1624 1578 Fragments arranged by size: 1624 1578 1163 1134 690 BtsI GCAGTG_nn' Cuts at: 0 834 2373 2690 3396 4219 4488 6123 6141 Size: 834 1539 317 706 823 269 1635 18 Cuts at: 6141 6189 Size: 48 Fragments arranged by size: 1635 1539 834 823 706 317 269 48 18 ClaI AT'CG_AT Cuts at: 0 3494 6189 Size: 3494 2695 DraIII CAC_nnn'GTG Cuts at: 0 2110 6189 Size: 2110 4079 DrdI GACnn_nn'nnGTC Cuts at: 0 313 3943 6189 Size: 313 3630 2246 Fragments arranged by size: 3630 2246 313 EaeI y'GGCC_r Cuts at: 0 133 197 326 341 595 813 855 1115 Size: 133 64 129 15 254 218 42 260 Cuts at: 1115 1142 3209 3530 5363 5895 5910 6189 Size: 27 2067 321 1833 532 15 279 Fragments arranged by size: 2067 1833 532 321 279 260 254 218 133 129 64 42 27 15 15 EagI C'GGCC_G Cuts at: 0 133 326 341 595 6189 Size: 133 193 15 254 5594 Fragments arranged by size: 5594 254 193 133 15 EarI CTCTTCn'nnn_ Cuts at: 0 400 755 2237 2366 3928 6189 Size: 400 355 1482 129 1562 2261 Fragments arranged by size: 2261 1562 1482 400 355 129 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 1034 2107 2743 3796 4629 6189 Size: 19 1015 1073 636 1053 833 1560 Fragments arranged by size: 1560 1073 1053 1015 833 636 19 EcoICRI GAG'CTC Cuts at: 0 1478 1710 1905 3357 5216 6189 Size: 1478 232 195 1452 1859 973 Fragments arranged by size: 1859 1478 1452 973 232 195 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 76 84 396 732 806 1337 1711 2126 Size: 76 8 312 336 74 531 374 415 Cuts at: 2126 2261 2555 2844 3307 3382 3403 3691 3733 Size: 135 294 289 463 75 21 288 42 Cuts at: 3733 3826 3979 4327 4474 4706 4968 5130 5287 Size: 93 153 348 147 232 262 162 157 Cuts at: 5287 5566 5589 6037 6189 Size: 279 23 448 152 Fragments arranged by size: 531 463 448 415 374 348 336 312 294 289 288 279 262 232 162 157 153 152 147 135 93 76 75 74 42 23 21 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 2668 3265 3385 3712 3868 4141 4870 5896 Size: 2668 597 120 327 156 273 729 1026 Cuts at: 5896 6189 Size: 293 Fragments arranged by size: 2668 1026 729 597 327 293 273 156 120 EcoO109I rG'GnC_Cy Cuts at: 0 191 299 348 677 768 1060 1312 1723 Size: 191 108 49 329 91 292 252 411 Cuts at: 1723 1912 2076 2195 2416 2529 2530 2660 2661 Size: 189 164 119 221 113 1 130 1 Cuts at: 2661 3054 3663 3846 4146 4227 4251 5181 5412 Size: 393 609 183 300 81 24 930 231 Cuts at: 5412 5995 6046 6189 Size: 583 51 143 Fragments arranged by size: 930 609 583 411 393 329 300 292 252 231 221 191 189 183 164 143 130 119 113 108 91 81 51 49 24 1 1 EcoRV GAT'ATC Cuts at: 0 1398 2386 6189 Size: 1398 988 3803 Fragments arranged by size: 3803 1398 988 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1143 6189 Size: 1143 5046 FspI TGC'GCA Cuts at: 0 388 3256 6189 Size: 388 2868 2933 Fragments arranged by size: 2933 2868 388 HaeII r_GCGC'y Cuts at: 0 63 264 428 2223 2775 3604 3789 4273 Size: 63 201 164 1795 552 829 185 484 Cuts at: 4273 5779 6189 Size: 1506 410 Fragments arranged by size: 1795 1506 829 552 484 410 201 185 164 63 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 274 306 421 453 1198 1230 1387 1419 Size: 274 32 115 32 745 32 157 32 Cuts at: 1419 1507 1539 1602 1634 2117 2149 2328 2360 Size: 88 32 63 32 483 32 179 32 Cuts at: 2360 2722 2754 3801 3833 4897 4909 4929 4941 Size: 362 32 1047 32 1064 12 20 12 Cuts at: 4941 5504 5536 6132 6164 6189 Size: 563 32 596 32 25 Fragments arranged by size: 1064 1047 745 596 563 483 362 274 179 157 115 88 63 32 32 32 32 32 32 32 32 32 32 32 32 25 20 12 12 HincII GTy'rAC Cuts at: 0 1369 5196 6189 Size: 1369 3827 993 Fragments arranged by size: 3827 1369 993 KpnI G_GTAC'C Cuts at: 0 1991 6189 Size: 1991 4198 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 4238 5291 6132 6189 Size: 4238 1053 841 57 Fragments arranged by size: 4238 1053 841 57 MscI TGG'CCA Cuts at: 0 1117 1144 3211 3532 5365 6189 Size: 1117 27 2067 321 1833 824 Fragments arranged by size: 2067 1833 1117 824 321 27 MslI CAynn'nnrTG Cuts at: 0 382 1996 2879 2896 3047 3262 3794 3886 Size: 382 1614 883 17 151 215 532 92 Cuts at: 3886 5467 6189 Size: 1581 722 Fragments arranged by size: 1614 1581 883 722 532 382 215 151 92 17 MspA1I CmG'CkG Cuts at: 0 295 325 331 631 780 863 1163 1374 Size: 295 30 6 300 149 83 300 211 Cuts at: 1374 1746 1890 2607 3285 3633 3723 4062 4237 Size: 372 144 717 678 348 90 339 175 Cuts at: 4237 4523 4771 5544 5582 5679 5861 5940 6189 Size: 286 248 773 38 97 182 79 249 Fragments arranged by size: 773 717 678 372 348 339 300 300 295 286 249 248 211 182 175 149 144 97 90 83 79 38 30 6 NaeI GCC'GGC Cuts at: 0 133 595 6189 Size: 133 462 5594 Fragments arranged by size: 5594 462 133 NcoI C'CATG_G Cuts at: 0 1658 2040 2321 6189 Size: 1658 382 281 3868 Fragments arranged by size: 3868 1658 382 281 NgoMIV G'CCGG_C Cuts at: 0 131 593 6189 Size: 131 462 5596 Fragments arranged by size: 5596 462 131 NotI GC'GGCC_GC Cuts at: 0 326 6189 Size: 326 5863 NspI r_CATG'y Cuts at: 0 527 3033 3081 3198 3279 3405 3418 3579 Size: 527 2506 48 117 81 126 13 161 Cuts at: 3579 4491 5311 5556 6189 Size: 912 820 245 633 Fragments arranged by size: 2506 912 820 633 527 245 161 126 117 81 48 13 PasI CC'CwG_GG Cuts at: 0 2047 2427 4158 5448 6189 Size: 2047 380 1731 1290 741 Fragments arranged by size: 2047 1731 1290 741 380 PciI A'CATG_T Cuts at: 0 5552 6189 Size: 5552 637 PflMI CCAn_nnn'nTGG Cuts at: 0 634 1812 4209 5080 5125 6189 Size: 634 1178 2397 871 45 1064 Fragments arranged by size: 2397 1178 1064 871 634 45 PfoI T'CCnGG_A Cuts at: 0 412 1022 1760 3359 3380 6189 Size: 412 610 738 1599 21 2809 Fragments arranged by size: 2809 1599 738 610 412 21 PmlI CAC'GTG Cuts at: 0 3207 5253 5646 6189 Size: 3207 2046 393 543 Fragments arranged by size: 3207 2046 543 393 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3028 3060 6189 Size: 3028 32 3129 Fragments arranged by size: 3129 3028 32 PpuMI rG'GwC_Cy Cuts at: 0 299 1060 1312 2195 3054 3663 4146 4227 Size: 299 761 252 883 859 609 483 81 Cuts at: 4227 4251 5181 5412 6189 Size: 24 930 231 777 Fragments arranged by size: 930 883 859 777 761 609 483 299 252 231 81 24 PspOMI G'GGCC_C Cuts at: 0 190 348 677 768 2075 2529 2660 2846 Size: 190 158 329 91 1307 454 131 186 Cuts at: 2846 6189 Size: 3343 Fragments arranged by size: 3343 1307 454 329 190 186 158 131 91 PspXI vC'TCGA_Gb Cuts at: 0 3007 6189 Size: 3007 3182 PstI C_TGCA'G Cuts at: 0 919 3147 3178 3806 4679 4776 4955 5543 Size: 919 2228 31 628 873 97 179 588 Cuts at: 5543 5934 6189 Size: 391 255 Fragments arranged by size: 2228 919 873 628 588 391 255 179 97 31 PvuI CG_AT'CG Cuts at: 0 3899 6189 Size: 3899 2290 PvuII CAG'CTG Cuts at: 0 780 4237 4523 5544 5679 5861 5940 6189 Size: 780 3457 286 1021 135 182 79 249 Fragments arranged by size: 3457 1021 780 286 249 182 135 79 SacI G_AGCT'C Cuts at: 0 1480 1712 1907 3359 5218 6189 Size: 1480 232 195 1452 1859 971 Fragments arranged by size: 1859 1480 1452 971 232 195 SacII CC_GC'GG Cuts at: 0 326 332 1164 6189 Size: 326 6 832 5025 Fragments arranged by size: 5025 832 326 6 SalI G'TCGA_C Cuts at: 0 1367 5194 6189 Size: 1367 3827 995 Fragments arranged by size: 3827 1367 995 SanDI GG'GwC_CC Cuts at: 0 299 5412 6189 Size: 299 5113 777 Fragments arranged by size: 5113 777 299 SapI GCTCTTCn'nnn_ Cuts at: 0 400 2237 6189 Size: 400 1837 3952 Fragments arranged by size: 3952 1837 400 SexAI A'CCwGG_T Cuts at: 0 1467 2106 2625 5345 6189 Size: 1467 639 519 2720 844 Fragments arranged by size: 2720 1467 844 639 519 SfcI C'TryA_G Cuts at: 0 915 2190 3143 3174 3802 4675 4772 4951 Size: 915 1275 953 31 628 873 97 179 Cuts at: 4951 5539 5930 6189 Size: 588 391 259 Fragments arranged by size: 1275 953 915 873 628 588 391 259 179 97 31 SmaI CCC'GGG Cuts at: 0 78 151 682 879 4671 5167 6189 Size: 78 73 531 197 3792 496 1022 Fragments arranged by size: 3792 1022 531 496 197 78 73 SmlI C'TyrA_G Cuts at: 0 111 1195 1600 3007 3556 5176 6189 Size: 111 1084 405 1407 549 1620 1013 Fragments arranged by size: 1620 1407 1084 1013 549 405 111 SphI G_CATG'C Cuts at: 0 3405 3418 3579 4491 5311 6189 Size: 3405 13 161 912 820 878 Fragments arranged by size: 3405 912 878 820 161 13 SrfI GCCC'GGGC Cuts at: 0 682 879 4671 6189 Size: 682 197 3792 1518 Fragments arranged by size: 3792 1518 682 197 StuI AGG'CCT Cuts at: 0 888 921 2188 3546 4393 4621 5206 5891 Size: 888 33 1267 1358 847 228 585 685 Cuts at: 5891 6063 6189 Size: 172 126 Fragments arranged by size: 1358 1267 888 847 685 585 228 172 126 33 StyI C'CwwG_G Cuts at: 0 1118 1658 2040 2321 3458 3533 4188 4377 Size: 1118 540 382 281 1137 75 655 189 Cuts at: 4377 5015 5438 6034 6189 Size: 638 423 596 155 Fragments arranged by size: 1137 1118 655 638 596 540 423 382 281 189 155 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 592 854 1804 5461 5470 5602 5610 5793 Size: 592 262 950 3657 9 132 8 183 Cuts at: 5793 6189 Size: 396 Fragments arranged by size: 3657 950 592 396 262 183 132 9 8 TatI w'GTAC_w Cuts at: 0 3026 4001 4037 6189 Size: 3026 975 36 2152 Fragments arranged by size: 3026 2152 975 36 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 5363 5395 5785 5817 6013 6045 6189 Size: 5363 32 390 32 196 32 144 Fragments arranged by size: 5363 390 196 144 32 32 32 XbaI T'CTAG_A Cuts at: 0 4437 6189 Size: 4437 1752 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1828 2044 2319 3219 4413 5466 5944 6189 Size: 1828 216 275 900 1194 1053 478 245 Fragments arranged by size: 1828 1194 1053 900 478 275 245 216 XhoI C'TCGA_G Cuts at: 0 3007 6189 Size: 3007 3182 XmaI C'CCGG_G Cuts at: 0 76 149 680 877 4669 5165 6189 Size: 76 73 531 197 3792 496 1024 Fragments arranged by size: 3792 1024 531 496 197 76 73 ZraI GAC'GTC Cuts at: 0 3015 4761 5694 6189 Size: 3015 1746 933 495 Fragments arranged by size: 3015 1746 933 495 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AfeI AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NotI NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SexAI SfcI SmaI SmlI SphI SrfI StuI StyI TaqII TatI TstI XbaI XcmI XhoI XmaI ZraI Enzymes that do not cut: AclI AflII ApoI AscI AseI AsiSI AvrII BaeI BbeI BciVI BfrBI BmtI BsaBI BsiWI BsmI BspEI BstBI BstZ17I CspCI DraI EcoRI FseI FspAI HindIII HpaI KasI MfeI MluI NarI NdeI NheI NruI NsiI PacI PmeI PshAI PsiI PsrI RsrII SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SspI SwaI Tth111I XmnI