Restriction map for pF1KE5136
##################################
# NO CUT ENZYMES (  159 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AfaI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AvaII       AvrII       
BamHI       BbrPI       BbsI        BbvCI       BciVI       
BclI        BfaI        BfrI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr13I      ClaI        CpoI        DraII       
DraIII      DrdI        Eam1105I    EarI        Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HincII      HindII      
HindIII     HinfI       HpaI        HphI        KpnI        
Ksp632I     MaeI        MaeII       MamI        MboII       
MfeI        MluI        MroI        MunI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsaI        
RsrII       SalI        SapI        Sau96I      SbfI        
ScaI        SexAI       SfiI        SfuI        SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TaqI        TfiI        
Tsp509I     Tth111I     TthHB8I     XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

AccI           30  GTCTAC               GT^[AC][GT]AC
AcyI           45  GGCGCC               G[GA]^CG[CT]C
AlwI          153  GATCC                GGATC(4/5)
AlwNI         205  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI        85  AGCGCT               AGC^GCT
AspHI         258  GAGCTC               G[AT]GC[AT]^C
AviII         225  TGCGCA               TGC^GCA
BalI           62  TGGCCA               TGG^CCA
BanII         258  GAGCTC               G[GA]GC[CT]^C
BbeI           45  GGCGCC               GGCGC^C
BbvI           73  GCAGC                GCAGC(8/12)
BcgI           15  GCAAACTGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          130  CCGGG                CC^[GC]GG
BglI           64  GCCACTAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI          45  GGCGCC               G[GA]^CG[CT]C
BsiEI         127  CGGCCG               CG[GA][CT]^CG
BsiHKAI       258  GAGCTC               G[AT]GC[AT]^C
BspMI         245  ACCTGC               ACCTGC(4/8)
BsrI           34  ACTGG                ACTGG(1/-1)
BstNI         210  CCTGG                CC^[AT]GG
BstYI         152  AGATCC               [GA]^GATC[CT]
DpnII         153  GATC                 ^GATC
DraI          235  TTTAAA               TTT^AAA
EagI          127  CGGCCG               C^GGCCG
EclXI         127  CGGCCG               C^GGCCG
Eco47III       85  AGCGCT               AGC^GCT
Eco52I        127  CGGCCG               C^GGCCG
EcoRII        210  CCTGG                ^CC[AT]GG
EcoT14I       365  CCAAGG               C^C[AT][AT]GG
FspI          225  TGCGCA               TGC^GCA
HgaI          350  GCGTC                GACGC(5/10)
Hin1I          45  GGCGCC               G[GA]^CG[CT]C
KasI           45  GGCGCC               G^GCGCC
KspI          393  CCGCGG               CCGC^GG
MaeIII        352  GTCAC                ^GT[ACGT]AC
MboI          153  GATC                 ^GATC
MflI          152  AGATCC               [GA]^GATC[CT]
MluNI          62  TGGCCA               TGG^CCA
MscI           62  TGGCCA               TGG^CCA
MseI          236  TTAA                 T^TAA
MslI          354  CACTATCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI          210  CCTGG                CC^[AT]GG
NarI           45  GGCGCC               GG^CGCC
NciI          130  CCGGG                CC^[GC]GG
NdeII         153  GATC                 ^GATC
NlaIII        360  CATG                 CATG^
PflMI         204  CCAGCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          277  CTGCAG               CTGCA^G
SacI          258  GAGCTC               GAGCT^C
SacII         393  CCGCGG               CCGC^GG
Sau3AI        153  GATC                 ^GATC
SfaNI         389  GCATC                GCATC(5/9)
SfcI          277  CTGCAG               C^T[GA][CT]AG
SfoI           45  GGCGCC               GGC^GCC
SgrAI          96  CACCGGCG             C[GA]^CCGG[CT]G
StuI          293  AGGCCT               AGG^CCT
StyI          365  CCAAGG               C^C[AT][AT]GG
Tru9I         236  TTAA                 T^TAA
TseI           73  GCAGC                G^C[AT]GC
Tsp45I        352  GTCAC                ^GT[GC]AC
TspRI         174  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I        204  CCAGCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         152  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AluI          259  AGCT                 AG^CT
              377  AGCT                
BanI           45  GGCGCC               G^G[CT][GA]CC
              133  GGCACC              
BmyI          132  GGGCAC               G[AGT]GC[ACT]^C
              258  GAGCTC              
BsiYI          91  CCGGCCACCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              204  CCAGCGCCTGG         
BslI           91  CCGGCCACCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              204  CCAGCGCCTGG         
Bsp1286I      132  GGGCAC               G[AGT]GC[ACT]^C
              258  GAGCTC              
BsrFI          97  ACCGGC               [GA]^CCGG[CT]
              125  ACCGGC              
BssKI         130  CCGGG                ^CC[ACGT]GG
              210  CCTGG               
Cfr10I         97  ACCGGC               [GA]^CCGG[CT]
              125  ACCGGC              
DdeI           11  CTAAG                C^T[ACGT]AG
               68  CTAAG               
DsaI          137  CCGTGG               C^C[GA][CT]GG
              393  CCGCGG              
FauI           77  CCCGC                CCCGC(4/6)
              383  CCCGC               
MspA1I        263  CCGCTG               C[AC]G^C[GT]G
              393  CCGCGG              
NlaIV          45  GGCGCC               GG[ACGT]^[ACGT]CC
              133  GGCACC              
ScrFI         130  CCGGG                CC^[ACGT]GG
              210  CCTGG               


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AccII       AciI        BsaJI       BstF5I      BstUI       
Cac8I       CfoI        EaeI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HpaII       ItaI        MnlI        MspI        MvnI        
MwoI        
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