Restriction map for pF1KE5136
 (Circular) (Six-base) MAPSORT of: pF1KE5136.seq  Check: 9585  from: 1  to: 3521



pF1KE5136  3521 bp

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:50  ..

AccI GT'mk_AC

Cuts at:    139     559     139 
   Size:        420    3101
 
Acc65I G'GTAC_C

Cuts at:    537     537 
   Size:       3521
 
AclI AA'CG_TT

Cuts at:   3361    3361 
   Size:       3521
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1346    1778    2525    2793    1346 
   Size:        432     747     268    2074

  Fragments arranged by size: 

                2074     747     432     268
 
AfeI AGC'GCT

Cuts at:    195     195 
   Size:       3521
 
AflIII A'CryG_T

Cuts at:    873    1977    2954     873 
   Size:       1104     977    1440

  Fragments arranged by size: 

                1440    1104     977
 
AgeI A'CCGG_T

Cuts at:   1909    1909 
   Size:       3521
 
AlwNI CAG_nnn'CTG

Cuts at:    318     826    2393     318 
   Size:        508    1567    1446

  Fragments arranged by size: 

                1567    1446     508
 
ApaLI G'TGCA_C

Cuts at:   2291    2291 
   Size:       3521
 
ApoI r'AATT_y

Cuts at:    525    2874    3130     525 
   Size:       2349     256     916

  Fragments arranged by size: 

                2349     916     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3521
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3521
 
AvaI C'yCGr_G

Cuts at:    541     541 
   Size:       3521
 
BamHI G'GATC_C

Cuts at:    546     546 
   Size:       3521
 
BanI G'GyrC_C

Cuts at:    153     241     537     951    1199    1234     153 
   Size:         88     296     414     248      35    2440

  Fragments arranged by size: 

                2440     414     296     248      88      35
 
BanII G_rGCy'C

Cuts at:    370     535    1565     370 
   Size:        165    1030    2326

  Fragments arranged by size: 

                2326    1030     165
 
BbeI G_GCGC'C

Cuts at:    157     955    1203     157 
   Size:        798     248    2475

  Fragments arranged by size: 

                2475     798     248
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    112     146     112 
   Size:         34    3487
 
BciVI GTATCCnnnnn_n'

Cuts at:   1419    2180    3521    1419 
   Size:        761    1341    1419

  Fragments arranged by size: 

                1419    1341     761
 
BclI T'GATC_A

Cuts at:   1041    1041 
   Size:       3521
 
BglI GCCn_nnn'nGGC

Cuts at:    178     178 
   Size:       3521
 
BglII A'GATC_T

Cuts at:   1036    3073    1036 
   Size:       2037    1484
 
BlpI GC'TnA_GC

Cuts at:    633     633 
   Size:       3521
 
Bme1580I G_kGCm'C

Cuts at:    244    1146    1239    2295     244 
   Size:        902      93    1056    1470

  Fragments arranged by size: 

                1470    1056     902      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    733     814    1149    3293     733 
   Size:         81     335    2144     961

  Fragments arranged by size: 

                2144     961     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1888    1888 
   Size:       3521
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    697    1576    2068    2366     697 
   Size:        879     492     298    1852

  Fragments arranged by size: 

                1852     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3225    3225 
   Size:       3521
 
BsaAI yAC'GTr

Cuts at:    874     874 
   Size:       3521
 
BsaBI GATnn'nnATC

Cuts at:      6    1035    3072       6 
   Size:       1029    2037     455

  Fragments arranged by size: 

                2037    1029     455
 
BsaHI Gr'CG_yC

Cuts at:    154     952    1200    1902    3398     154 
   Size:        798     248     702    1496     277

  Fragments arranged by size: 

                1496     798     702     277     248
 
BsaWI w'CCGG_w

Cuts at:    710    1231    1909    2183    2330    2741     710 
   Size:        521     678     274     147     411    1490

  Fragments arranged by size: 

                1490     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    946    1704    2281    2777     946 
   Size:        758     577     496    1690

  Fragments arranged by size: 

                1690     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     238    1063    1109    2317     104 
   Size:        134     825      46    1208    1308

  Fragments arranged by size: 

                1308    1208     825     134      46
 
BsiHKAI G_wGCw'C

Cuts at:    370     535    1313    1503    2295     370 
   Size:        165     778     190     792    1596

  Fragments arranged by size: 

                1596     792     778     190     165
 
Bsp1286I G_dGCh'C

Cuts at:    244     370     535    1146    1239    1313    1503    1565    2295 
   Size:        126     165     611      93      74     190      62     730

Cuts at:   2295     244 
   Size:       1470

  Fragments arranged by size: 

                1470     730     611     190     165     126      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:   2741    2741 
   Size:       3521
 
BspHI T'CATG_A

Cuts at:   2697    2697 
   Size:       3521
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    362     571     785    1087    1468     362 
   Size:        209     214     302     381    2415

  Fragments arranged by size: 

                2415     381     302     214     209
 
BsrBI CCG'CTC

Cuts at:     50    1813    1867    3351    3516      50 
   Size:       1763      54    1484     165      55

  Fragments arranged by size: 

                1763    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1433    1433 
   Size:       3521
 
BsrFI r'CCGG_y

Cuts at:    205     233    1519    1700    1909     205 
   Size:         28    1286     181     209    1817

  Fragments arranged by size: 

                1817    1286     209     181      28
 
BssSI C'ACGA_G

Cuts at:   1792    2150    1792 
   Size:        358    3163
 
BstAPI GCAn_nnn'nTGC

Cuts at:    898     898 
   Size:       3521
 
BstBI TT'CG_AA

Cuts at:   1882    1882 
   Size:       3521
 
BstYI r'GATC_y

Cuts at:      1     260     546    1013    1036    1371    1617    2629    2715 
   Size:        259     286     467      23     335     246    1012      86

Cuts at:   2715    2727    3073       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     467     449     346     335     286     259     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     245     501     107 
   Size:        138     256    3127

  Fragments arranged by size: 

                3127     256     138
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1471    1652    1815    1471 
   Size:        181     163    3177

  Fragments arranged by size: 

                3177     181     163
 
BtsI GCAGTG_nn'

Cuts at:    764     764 
   Size:       3521
 
DraI TTT'AAA

Cuts at:    345     520     345 
   Size:        175    3346
 
DrdI GACnn_nn'nnGTC

Cuts at:   1227    2085    1227 
   Size:        858    2663
 
EaeI y'GGCC_r

Cuts at:    170     200     235    1106    1280    1671    1698    3043     170 
   Size:         30      35     871     174     391      27    1345     648

  Fragments arranged by size: 

                1345     871     648     391     174      35      30      27
            
 
EagI C'GGCC_G

Cuts at:    235    1106     235 
   Size:        871    2650
 
EarI CTCTTCn'nnn_

Cuts at:   1544    1754    1544 
   Size:        210    3311
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    253    1729    2039    2185    3331     253 
   Size:       1476     310     146    1146     443

  Fragments arranged by size: 

                1476    1146     443     310     146
 
EcoICRI GAG'CTC

Cuts at:    368     533     368 
   Size:        165    3356
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1346    1778    2525    2793    1346 
   Size:        432     747     268    2074

  Fragments arranged by size: 

                2074     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    660     660 
   Size:       3521
 
EcoRI G'AATT_C

Cuts at:    525     525 
   Size:       3521
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1540    1540 
   Size:       3521
 
FspI TGC'GCA

Cuts at:    335    1302     335 
   Size:        967    2554
 
HaeII r_GCGC'y

Cuts at:    157     197     318     955    1203    2225    3204     157 
   Size:         40     121     637     248    1022     979     474

  Fragments arranged by size: 

                1022     979     637     474     248     121      40
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1363    1393    1395    1425    1363 
   Size:         30       2      30    3459

  Fragments arranged by size: 

                3459      30      30       2
 
HincII GTy'rAC

Cuts at:    560     560 
   Size:       3521
 
KasI G'GCGC_C

Cuts at:    153     951    1199     153 
   Size:        798     248    2475

  Fragments arranged by size: 

                2475     798     248
 
KpnI G_GTAC'C

Cuts at:    541     541 
   Size:       3521
 
MluI A'CGCG_T

Cuts at:   2954    2954 
   Size:       3521
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2192    2376    2192 
   Size:        184    3337
 
MscI TGG'CCA

Cuts at:    172    1282     172 
   Size:       1110    2411
 
MslI CAynn'nnrTG

Cuts at:    466    1510    1637     466 
   Size:       1044     127    2350

  Fragments arranged by size: 

                2350    1044     127
 
MspA1I CmG'CkG

Cuts at:    373     503     632     946    1306    2319    2564     373 
   Size:        130     129     314     360    1013     245    1330

  Fragments arranged by size: 

                1330    1013     360     314     245     130     129
 
NaeI GCC'GGC

Cuts at:   1702    1702 
   Size:       3521
 
NarI GG'CG_CC

Cuts at:    154     952    1200     154 
   Size:        798     248    2475

  Fragments arranged by size: 

                2475     798     248
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3521
 
NgoMIV G'CCGG_C

Cuts at:   1700    1700 
   Size:       3521
 
NspI r_CATG'y

Cuts at:    574    1074    1981     574 
   Size:        500     907    2114

  Fragments arranged by size: 

                2114     907     500
 
PciI A'CATG_T

Cuts at:   1977    1977 
   Size:       3521
 
PflMI CCAn_nnn'nTGG

Cuts at:    318     318 
   Size:       3521
 
PmeI GTTT'AAAC

Cuts at:    520     520 
   Size:       3521
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2660    2692    2660 
   Size:         32    3489
 
PstI C_TGCA'G

Cuts at:    389     568    1253     389 
   Size:        179     685    2657

  Fragments arranged by size: 

                2657     685     179
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3521
 
PvuII CAG'CTG

Cuts at:    946    1306     946 
   Size:        360    3161
 
RsrII CG'GwC_CG

Cuts at:   1716    1716 
   Size:       3521
 
SacI G_AGCT'C

Cuts at:    370     535     370 
   Size:        165    3356
 
SacII CC_GC'GG

Cuts at:    504     504 
   Size:       3521
 
SalI G'TCGA_C

Cuts at:    558     558 
   Size:       3521
 
SapI GCTCTTCn'nnn_

Cuts at:   1544    1754    1544 
   Size:        210    3311
 
SbfI CC_TGCA'GG

Cuts at:    568     568 
   Size:       3521
 
ScaI AGT'ACT

Cuts at:   3038    3038 
   Size:       3521
 
SfcI C'TryA_G

Cuts at:     33     385     564    1249    2242    2433      33 
   Size:        352     179     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     352     191     179
 
SfoI GGC'GCC

Cuts at:    155     953    1201     155 
   Size:        798     248    2475

  Fragments arranged by size: 

                2475     798     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3521
 
SgrAI Cr'CCGG_yG

Cuts at:    205     205 
   Size:       3521
 
SmaI CCC'GGG

Cuts at:    543     543 
   Size:       3521
 
SmlI C'TyrA_G

Cuts at:    676    1591    2083    2345     676 
   Size:        915     492     262    1852

  Fragments arranged by size: 

                1852     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3521
 
SphI G_CATG'C

Cuts at:    574    1074     574 
   Size:        500    3021
 
StuI AGG'CCT

Cuts at:    403     403 
   Size:       3521
 
StyI C'CwwG_G

Cuts at:    107     473     655    1009     107 
   Size:        366     182     354    2619

  Fragments arranged by size: 

                2619     366     354     182
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1104    1237    1696    1905    1104 
   Size:        133     459     209    2720

  Fragments arranged by size: 

                2720     459     209     133
 
TatI w'GTAC_w

Cuts at:   3036    3036 
   Size:       3521
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3038    3070    3038 
   Size:         32    3489
 
Tth111I GACn'n_nGTC

Cuts at:   1318    1318 
   Size:       3521
 
XbaI T'CTAG_A

Cuts at:    552     552 
   Size:       3521
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1016    1016 
   Size:       3521
 
XmaI C'CCGG_G

Cuts at:    541     541 
   Size:       3521
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PciI    PflMI     PmeI     PpiI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI    SgrAI     SmaI     SmlI
     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI    BfrBI    BmgBI     BmtI
     BpmI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsmBI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI
   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PfoI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI    SanDI
    SexAI     SfiI    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com