Restriction map for pF1KE2553
 (Circular) (Six-base) MAPSORT of: pF1K.seq  Check: 1693  from: 1  to: 3450




 With 182 enzymes: SgfI * 

                        September 4, 2008 14:04  ..

AatII G_ACGT'C

Cuts at:    245     245 
   Size:       3450
 
AccI GT'mk_AC

Cuts at:    488     488 
   Size:       3450
 
Acc65I G'GTAC_C

Cuts at:    466     466 
   Size:       3450
 
AclI AA'CG_TT

Cuts at:   3290    3290 
   Size:       3450
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    136     339    1275    1707    2454    2722     136 
   Size:        203     936     432     747     268     864

  Fragments arranged by size: 

                 936     864     747     432     268     203
 
AflIII A'CryG_T

Cuts at:    125     802    1906    2883     125 
   Size:        677    1104     977     692

  Fragments arranged by size: 

                1104     977     692     677
 
AgeI A'CCGG_T

Cuts at:   1838    1838 
   Size:       3450
 
AlwNI CAG_nnn'CTG

Cuts at:    755    2322     755 
   Size:       1567    1883
 
ApaLI G'TGCA_C

Cuts at:   2220    2220 
   Size:       3450
 
ApoI r'AATT_y

Cuts at:    360     454    2803    3059     360 
   Size:         94    2349     256     751

  Fragments arranged by size: 

                2349     751     256      94
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3450
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3450
 
AvaI C'yCGr_G

Cuts at:    470     470 
   Size:       3450
 
BamHI G'GATC_C

Cuts at:    475     475 
   Size:       3450
 
BanI G'GyrC_C

Cuts at:    466     880    1128    1163     466 
   Size:        414     248      35    2753

  Fragments arranged by size: 

                2753     414     248      35
 
BanII G_rGCy'C

Cuts at:    464    1494     464 
   Size:       1030    2420
 
BbeI G_GCGC'C

Cuts at:    884    1132     884 
   Size:        248    3202
 
BciVI GTATCCnnnnn_n'

Cuts at:   1348    2109    3450    1348 
   Size:        761    1341    1348

  Fragments arranged by size: 

                1348    1341     761
 
BclI T'GATC_A

Cuts at:    970     970 
   Size:       3450
 
BglII A'GATC_T

Cuts at:    965    3002     965 
   Size:       2037    1413
 
BlpI GC'TnA_GC

Cuts at:    562     562 
   Size:       3450
 
Bme1580I G_kGCm'C

Cuts at:   1075    1168    2224    1075 
   Size:         93    1056    2301

  Fragments arranged by size: 

                2301    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    662     743    1078    3222     662 
   Size:         81     335    2144     890

  Fragments arranged by size: 

                2144     890     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1817    1817 
   Size:       3450
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    283     283 
   Size:       3450
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    366     626    1505    1997    2295     366 
   Size:        260     879     492     298    1521

  Fragments arranged by size: 

                1521     879     492     298     260
 
BsaI GGTCTCn'nnnn_

Cuts at:   3154    3154 
   Size:       3450
 
BsaAI yAC'GTr

Cuts at:    803     803 
   Size:       3450
 
BsaBI GATnn'nnATC

Cuts at:      6     964    3001       6 
   Size:        958    2037     455

  Fragments arranged by size: 

                2037     958     455
 
BsaHI Gr'CG_yC

Cuts at:    242     881    1129    1831    3327     242 
   Size:        639     248     702    1496     365

  Fragments arranged by size: 

                1496     702     639     365     248
 
BsaWI w'CCGG_w

Cuts at:    368     639    1160    1838    2112    2259    2670     368 
   Size:        271     521     678     274     147     411    1148

  Fragments arranged by size: 

                1148     678     521     411     274     271     147
 
BseYI C'CCAG_C

Cuts at:    212     875    1633    2210    2706     212 
   Size:        663     758     577     496     956

  Fragments arranged by size: 

                 956     758     663     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     992    1038    2246     104 
   Size:        888      46    1208    1308

  Fragments arranged by size: 

                1308    1208     888      46
 
BsiHKAI G_wGCw'C

Cuts at:    464    1242    1432    2224     464 
   Size:        778     190     792    1690

  Fragments arranged by size: 

                1690     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    464    1075    1168    1242    1432    1494    2224     464 
   Size:        611      93      74     190      62     730    1690

  Fragments arranged by size: 

                1690     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2670    2670 
   Size:       3450
 
BspHI T'CATG_A

Cuts at:   2626    2626 
   Size:       3450
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    500     714    1016    1397     500 
   Size:        214     302     381    2553

  Fragments arranged by size: 

                2553     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1742    1796    3280    3445      50 
   Size:       1692      54    1484     165      55

  Fragments arranged by size: 

                1692    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1362    1362 
   Size:       3450
 
BsrFI r'CCGG_y

Cuts at:   1448    1629    1838    1448 
   Size:        181     209    3060

  Fragments arranged by size: 

                3060     209     181
 
BssSI C'ACGA_G

Cuts at:   1721    2079    1721 
   Size:        358    3092
 
BstAPI GCAn_nnn'nTGC

Cuts at:    827     827 
   Size:       3450
 
BstBI TT'CG_AA

Cuts at:   1811    1811 
   Size:       3450
 
BstYI r'GATC_y

Cuts at:      1     475     942     965    1300    1546    2558    2644    2656 
   Size:        474     467      23     335     246    1012      86      12

Cuts at:   2656    3002       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     474     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3450
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1400    1581    1744    1400 
   Size:        181     163    3106

  Fragments arranged by size: 

                3106     181     163
 
BtsI GCAGTG_nn'

Cuts at:    693     693 
   Size:       3450
 
DraI TTT'AAA

Cuts at:    449     449 
   Size:       3450
 
DrdI GACnn_nn'nnGTC

Cuts at:   1156    2014    1156 
   Size:        858    2592
 
EaeI y'GGCC_r

Cuts at:   1035    1209    1600    1627    2972    1035 
   Size:        174     391      27    1345    1513

  Fragments arranged by size: 

                1513    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1035    1035 
   Size:       3450
 
EarI CTCTTCn'nnn_

Cuts at:   1473    1683    1473 
   Size:        210    3240
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    281    1658    1968    2114    3260     281 
   Size:       1377     310     146    1146     471

  Fragments arranged by size: 

                1377    1146     471     310     146
 
EcoICRI GAG'CTC

Cuts at:    462     462 
   Size:       3450
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    136     283     339    1275    1707    2454    2722     136 
   Size:        147      56     936     432     747     268     864

  Fragments arranged by size: 

                 936     864     747     432     268     147      56
 
EcoO109I rG'GnC_Cy

Cuts at:    589     589 
   Size:       3450
 
EcoRI G'AATT_C

Cuts at:    454     454 
   Size:       3450
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    250    1469     250 
   Size:       1219    2231
 
FspI TGC'GCA

Cuts at:   1231    1231 
   Size:       3450
 
HaeII r_GCGC'y

Cuts at:    884    1132    2154    3133     884 
   Size:        248    1022     979    1201

  Fragments arranged by size: 

                1201    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    262     294    1292    1322    1324    1354     262 
   Size:         32     998      30       2      30    2358

  Fragments arranged by size: 

                2358     998      32      30      30       2
 
HincII GTy'rAC

Cuts at:    489     489 
   Size:       3450
 
KasI G'GCGC_C

Cuts at:    880    1128     880 
   Size:        248    3202
 
KpnI G_GTAC'C

Cuts at:    470     470 
   Size:       3450
 
MluI A'CGCG_T

Cuts at:   2883    2883 
   Size:       3450
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2121    2305    2121 
   Size:        184    3266
 
MscI TGG'CCA

Cuts at:   1211    1211 
   Size:       3450
 
MslI CAynn'nnrTG

Cuts at:   1439    1566    1439 
   Size:        127    3323
 
MspA1I CmG'CkG

Cuts at:    561     875    1235    2248    2493     561 
   Size:        314     360    1013     245    1518

  Fragments arranged by size: 

                1518    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1631    1631 
   Size:       3450
 
NarI GG'CG_CC

Cuts at:    881    1129     881 
   Size:        248    3202
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3450
 
NgoMIV G'CCGG_C

Cuts at:   1629    1629 
   Size:       3450
 
NspI r_CATG'y

Cuts at:    503    1003    1910     503 
   Size:        500     907    2043

  Fragments arranged by size: 

                2043     907     500
 
PciI A'CATG_T

Cuts at:   1906    1906 
   Size:       3450
 
PmeI GTTT'AAAC

Cuts at:    449     449 
   Size:       3450
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2589    2621    2589 
   Size:         32    3418
 
PstI C_TGCA'G

Cuts at:    497    1182     497 
   Size:        685    2765
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3450
 
PvuII CAG'CTG

Cuts at:    875    1235     875 
   Size:        360    3090
 
RsrII CG'GwC_CG

Cuts at:   1645    1645 
   Size:       3450
 
SacI G_AGCT'C

Cuts at:    464     464 
   Size:       3450
 
SalI G'TCGA_C

Cuts at:    487     487 
   Size:       3450
 
SapI GCTCTTCn'nnn_

Cuts at:   1473    1683    1473 
   Size:        210    3240
 
SbfI CC_TGCA'GG

Cuts at:    497     497 
   Size:       3450
 
ScaI AGT'ACT

Cuts at:   2967    2967 
   Size:       3450
 
SfcI C'TryA_G

Cuts at:     33     493    1178    2171    2362      33 
   Size:        460     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     460     191
 
SfoI GGC'GCC

Cuts at:    882    1130     882 
   Size:        248    3202
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3450
 
SmaI CCC'GGG

Cuts at:    472     472 
   Size:       3450
 
SmlI C'TyrA_G

Cuts at:    381     605    1520    2012    2274     381 
   Size:        224     915     492     262    1557

  Fragments arranged by size: 

                1557     915     492     262     224
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3450
 
SphI G_CATG'C

Cuts at:    503    1003     503 
   Size:        500    2950
 
StyI C'CwwG_G

Cuts at:    107     584     938     107 
   Size:        477     354    2619

  Fragments arranged by size: 

                2619     477     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    204    1033    1166    1625    1834     204 
   Size:        829     133     459     209    1820

  Fragments arranged by size: 

                1820     829     459     209     133
 
TatI w'GTAC_w

Cuts at:   2965    2965 
   Size:       3450
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2967    2999    2967 
   Size:         32    3418
 
Tth111I GACn'n_nGTC

Cuts at:   1247    1247 
   Size:       3450
 
XbaI T'CTAG_A

Cuts at:    481     481 
   Size:       3450
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    945     945 
   Size:       3450
 
XmaI C'CCGG_G

Cuts at:    470     470 
   Size:       3450
 
ZraI GAC'GTC

Cuts at:    243     243 
   Size:       3450
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PciI     PmeI     PpiI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI     BsmI
    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI
     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     XmnI
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