Restriction map for pF1KE2710
##################################
# NO CUT ENZYMES (  159 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BamHI       BanII       BbrPI       BbsI        
BbvCI       BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BpuAI       BsaAI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BsrI        BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Cfr10I      ClaI        CpoI        
DraI        DrdI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HgaI        HincII      HindII      HindIII     
HinfI       HpaI        HphI        KpnI        Ksp632I     
MaeI        MaeII       MaeIII      MboII       MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MslI        MunI        NaeI        NdeI        
NgoMIV      NheI        NotI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SalI        
SapI        SbfI        ScaI        SexAI       SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tru9I       Tsp45I      Tsp509I     Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AcyI          175  GGCGCC               G[GA]^CG[CT]C
AlwI          482  GGATC                GGATC(4/5)
AlwNI         447  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          524  CCTCAGG              CC^T[ACGT]AGG
Asp700I       369  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI          175  GGCGCC               GGCGC^C
BcgI          151  GCAAGGGCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI          514  GCTCAGC              GC^T[ACGT]AGC
BmyI           18  GGGCAC               G[AGT]GC[ACT]^C
BpmI          457  CTCCAG               CTGGAG(16/14)
Bpu1102I      514  GCTCAGC              GC^T[ACGT]AGC
BsaBI         477  GATGTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         175  GGCGCC               G[GA]^CG[CT]C
BseRI         170  GAGGAG               GAGGAG(10/8)
BsmAI         455  GTCTC                GTCTC(1/5)
BsmFI         131  GTCCC                GGGAC(10/14)
BsmI           65  GCATTC               GAATGC(1/-1)
Bsp1286I       18  GGGCAC               G[AGT]GC[ACT]^C
BsrDI          86  GCAATG               GCAATG(2/0)
Bsu36I        524  CCTCAGG              CC^T[ACGT]AGG
CelII         514  GCTCAGC              GC^T[ACGT]AGC
DpnII         483  GATC                 ^GATC
DraIII        486  CACGCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I        524  CCTCAGG              CC^T[ACGT]AGG
EcoT14I       296  CCATGG               C^C[AT][AT]GG
HaeII         175  GGCGCC               [GA]GCGC^[CT]
Hin1I         175  GGCGCC               G[GA]^CG[CT]C
KasI          175  GGCGCC               G^GCGCC
KspI          181  CCGCGG               CCGC^GG
MamI          477  GATGTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          483  GATC                 ^GATC
MspA1I        181  CCGCGG               C[AC]G^C[GT]G
NarI          175  GGCGCC               GG^CGCC
NcoI          296  CCATGG               C^CATGG
NdeII         483  GATC                 ^GATC
NruI          287  TCGCGA               TCG^CGA
PpuMI         129  AGGTCCC              [GA]G^G[AT]CC[CT]
SacII         181  CCGCGG               CCGC^GG
Sau3AI        483  GATC                 ^GATC
SfiI           49  GGCCCAGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          175  GGCGCC               GGC^GCC
SmaI          134  CCCGGG               CCC^GGG
SmlI          262  CTTGAG               C^T[CT][GA]AG
StyI          296  CCATGG               C^C[AT][AT]GG
TaqI           31  TCGA                 T^CGA
TthHB8I        31  TCGA                 T^CGA
XmaCI         134  CCCGGG               C^CCGGG
XmaI          134  CCCGGG               C^CCGGG
XmnI          369  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AvaI          134  CCCGGG               C^[CT]CG[GA]G
              159  CTCGGG              
AvaII         130  GGTCC                G^G[AT]CC
              187  GGTCC               
BanI          112  GGTGCC               G^G[CT][GA]CC
              175  GGCGCC              
BglI           50  GCCCAGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
               77  GCCCAGGGGGC         
BsoBI         134  CCCGGG               C^[CT]CG[GA]G
              159  CTCGGG              
BstF5I        220  GGATG                GGATG(2/0)
              333  GGATG               
DdeI          515  CTCAG                C^T[ACGT]AG
              525  CTCAG               
EcoNI         324  CCTGGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              520  CCTCCCTCAGG         
FokI          220  GGATG                GGATG(9/13)
              333  GGATG               
TspRI         378  CACTG                CA[GC]TG[ACGT][ACGT]^
              490  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AccII       AciI        AluI        BbvI        BcnI        
BsaJI       BsiYI       BslI        BssKI       BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DraII       
DsaI        EcoO109I    EcoRII      FauI        Fnu4HI      
HaeIII      HapII       HhaI        HinP1I      HpaII       
ItaI        MnlI        MspI        MvaI        MvnI        
MwoI        NciI        NlaIII      NlaIV       PstI        
Sau96I      ScrFI       SfaNI       SfcI        TseI        
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