Restriction map for pF1KE2710
 (Linear) (Six-base) MAPSORT of: KIEE2710.seq  Check: 6866  from: 1  to: 540



KIEE2710.orf

 With 182 enzymes: SgfI * 

                        January 5, 2018 10:00  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     191     388     540 
   Size:        191     197     152

  Fragments arranged by size: 

                 197     191     152
 
AlwNI CAG_nnn'CTG

Cuts at:      0     452     540 
   Size:        452      88
 
AvaI C'yCGr_G

Cuts at:      0     134     159     540 
   Size:        134      25     381

  Fragments arranged by size: 

                 381     134      25
 
BanI G'GyrC_C

Cuts at:      0     112     175     540 
   Size:        112      63     365

  Fragments arranged by size: 

                 365     112      63
 
BbeI G_GCGC'C

Cuts at:      0     179     540 
   Size:        179     361
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     140     174     540 
   Size:        140      34     366

  Fragments arranged by size: 

                 366     140      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      56      83     540 
   Size:         56      27     457

  Fragments arranged by size: 

                 457      56      27
 
BlpI GC'TnA_GC

Cuts at:      0     515     540 
   Size:        515      25
 
Bme1580I G_kGCm'C

Cuts at:      0      22     540 
   Size:         22     518
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     442     540 
   Size:        442      98
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     540 
   Size:        283     257
 
BsaBI GATnn'nnATC

Cuts at:      0     481     540 
   Size:        481      59
 
BsaHI Gr'CG_yC

Cuts at:      0     176     540 
   Size:        176     364
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     185     540 
   Size:        185     355
 
BsmI GAATG_Cn'

Cuts at:      0      65     540 
   Size:         65     475
 
Bsp1286I G_dGCh'C

Cuts at:      0      22     540 
   Size:         22     518
 
BsrDI GCAATG_nn'

Cuts at:      0      93     540 
   Size:         93     447
 
Bsu36I CC'TnA_GG

Cuts at:      0     525     540 
   Size:        525      15
 
BtgI C'CryG_G

Cuts at:      0     116     181     296     540 
   Size:        116      65     115     244

  Fragments arranged by size: 

                 244     116     115      65
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      53     540 
   Size:         53     487
 
BtsI GCAGTG_nn'

Cuts at:      0     496     540 
   Size:        496      44
 
DraIII CAC_nnn'GTG

Cuts at:      0     491     540 
   Size:        491      49
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     191     388     442     540 
   Size:        191     197      54      98

  Fragments arranged by size: 

                 197     191      98      54
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     328     524     540 
   Size:        328     196      16

  Fragments arranged by size: 

                 328     196      16
 
EcoO109I rG'GnC_Cy

Cuts at:      0      58     130     155     540 
   Size:         58      72      25     385

  Fragments arranged by size: 

                 385      72      58      25
 
HaeII r_GCGC'y

Cuts at:      0     179     540 
   Size:        179     361
 
KasI G'GCGC_C

Cuts at:      0     175     540 
   Size:        175     365
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     429     540 
   Size:        429     111
 
MspA1I CmG'CkG

Cuts at:      0     183     540 
   Size:        183     357
 
NarI GG'CG_CC

Cuts at:      0     176     540 
   Size:        176     364
 
NcoI C'CATG_G

Cuts at:      0     296     540 
   Size:        296     244
 
NruI TCG'CGA

Cuts at:      0     289     540 
   Size:        289     251
 
PasI CC'CwG_GG

Cuts at:      0      79     540 
   Size:         79     461
 
PpuMI rG'GwC_Cy

Cuts at:      0     130     540 
   Size:        130     410
 
PstI C_TGCA'G

Cuts at:      0     229     420     437     540 
   Size:        229     191      17     103

  Fragments arranged by size: 

                 229     191     103      17
 
SacII CC_GC'GG

Cuts at:      0     184     540 
   Size:        184     356
 
SfcI C'TryA_G

Cuts at:      0     225     416     433     540 
   Size:        225     191      17     107

  Fragments arranged by size: 

                 225     191     107      17
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      56     540 
   Size:         56     484
 
SfoI GGC'GCC

Cuts at:      0     177     540 
   Size:        177     363
 
SmaI CCC'GGG

Cuts at:      0     136     540 
   Size:        136     404
 
SmlI C'TyrA_G

Cuts at:      0     262     540 
   Size:        262     278
 
StyI C'CwwG_G

Cuts at:      0     296     540 
   Size:        296     244
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     309     540 
   Size:        309     231
 
XmaI C'CCGG_G

Cuts at:      0     134     540 
   Size:        134     406
 
XmnI GAAnn'nnTTC

Cuts at:      0     373     540 
   Size:        373     167
 

 Enzymes that do cut:

     AcuI    AlwNI     AvaI     BanI     BbeI     BcgI     BglI     BlpI
 Bme1580I     BpmI    BpuEI    BsaBI    BsaHI    BseRI     BsmI Bsp1286I
    BsrDI   Bsu36I     BtgI    BtgZI     BtsI   DraIII  Eco57MI    EcoNI
 EcoO109I    HaeII     KasI     MmeI   MspA1I     NarI     NcoI     NruI
     PasI    PpuMI     PstI    SacII     SfcI     SfiI     SfoI     SmaI
     SmlI     StyI    TaqII     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbsI
    BbvCI    BciVI     BclI    BfrBI    BglII    BmgBI     BmrI     BmtI
     BplI   Bpu10I     BsaI    BsaAI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MscI     MslI     NaeI     NdeI   NgoMIV     NheI     NotI     NsiI
     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     ZraI
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