################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MflI MluI MroI MseI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcsI 286 AAATTC [GA]^AATT[CT] AfaI 109 GTAC GT^AC AlwI 430 GATCC GGATC(4/5) AlwNI 274 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 286 AAATTC [GA]^AATT[CT] BalI 192 TGGCCA TGG^CCA BciVI 91 GGATAC GTATCC(6/5) BfaI 503 CTAG C^TAG BglI 46 GCCCGACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 313 GAGACC GGTCTC(1/5) BseRI 498 CTCCTC GAGGAG(10/8) BsiEI 49 CGACCG CG[GA][CT]^CG BsrFI 51 ACCGGC [GA]^CCGG[CT] BssSI 413 CTCGTG CACGAG(-5/-1) BstXI 189 CCATGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 438 GCCCGC GC[ACGT]^[ACGT]GC Cfr10I 51 ACCGGC [GA]^CCGG[CT] Cfr13I 377 GGCCC G^G[ACGT]CC DdeI 394 CTGAG C^T[ACGT]AG EaeI 192 TGGCCA [CT]^GGCC[GA] EarI 208 CTCTTC CTCTTC(1/4) EcoNI 389 CCTCTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 439 CCCGC CCCGC(4/6) HapII 52 CCGG C^CGG HpaII 52 CCGG C^CGG HphI 330 GGTGA GGTGA(8/7) Ksp632I 208 CTCTTC CTCTTC(1/4) MaeI 503 CTAG C^TAG MboII 209 TCTTC GAAGA(8/7) MfeI 257 CAATTG C^AATTG MluNI 192 TGGCCA TGG^CCA MscI 192 TGGCCA TGG^CCA MspA1I 440 CCGCTG C[AC]G^C[GT]G MspI 52 CCGG C^CGG MunI 257 CAATTG C^AATTG PshAI 315 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 422 CTGCAG CTGCA^G RsaI 109 GTAC GT^AC Sau96I 377 GGCCC G^G[ACGT]CC SfaNI 152 GCATC GCATC(5/9) SfcI 422 CTGCAG C^T[GA][CT]AG Tsp45I 506 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### BpmI 446 CTCCAG CTGGAG(16/14) 310 CTGGAG BsmAI 313 GAGAC GTCTC(1/5) 482 GAGAC BsmFI 519 GGGAC GGGAC(10/14) 322 GTCCC BssKI 156 CCTGG ^CC[ACGT]GG 528 CCAGG BstF5I 153 CATCC GGATG(2/0) 168 CATCC BstNI 156 CCTGG CC^[AT]GG 528 CCAGG DpnII 345 GATC ^GATC 430 GATC DsaI 189 CCATGG C^C[GA][CT]GG 195 CCATGG EcoRII 156 CCTGG ^CC[AT]GG 528 CCAGG EcoT14I 189 CCATGG C^C[AT][AT]GG 195 CCATGG FokI 153 CATCC GGATG(9/13) 168 CATCC HaeIII 193 GGCC GG^CC 377 GGCC HinfI 175 GAGTC G^A[ACGT]TC 521 GACTC MaeIII 8 GTAAC ^GT[ACGT]AC 506 GTGAC MboI 345 GATC ^GATC 430 GATC MslI 190 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 409 CACTCTCGTG MvaI 156 CCTGG CC^[AT]GG 528 CCAGG NcoI 189 CCATGG C^CATGG 195 CCATGG NdeII 345 GATC ^GATC 430 GATC PleI 521 GACTC GAGTC(4/5) 175 GAGTC Sau3AI 345 GATC ^GATC 430 GATC ScrFI 156 CCTGG CC^[ACGT]GG 528 CCAGG StyI 189 CCATGG C^C[AT][AT]GG 195 CCATGG TaqI 405 TCGA T^CGA 428 TCGA Tsp509I 258 AATT ^AATT 287 AATT TthHB8I 405 TCGA T^CGA 428 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AciI AluI BbvI BsaJI BsiYI BslI BsrI Fnu4HI ItaI MnlI MwoI NlaIII NlaIV TseI