(Circular) (Six-base) MAPSORT of: pF1KE2763.seq Check: 2447 from: 1 to: 3467 pF1KE2763 3467 bp With 182 enzymes: SgfI * October 3, 2019 17:18 .. AccI GT'mk_AC Cuts at: 505 505 Size: 3467 Acc65I G'GTAC_C Cuts at: 483 483 Size: 3467 AclI AA'CG_TT Cuts at: 3307 3307 Size: 3467 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 361 472 1292 1724 2471 2739 361 Size: 111 820 432 747 268 1089 Fragments arranged by size: 1089 820 747 432 268 111 AflIII A'CryG_T Cuts at: 819 1923 2900 819 Size: 1104 977 1386 Fragments arranged by size: 1386 1104 977 AgeI A'CCGG_T Cuts at: 1855 1855 Size: 3467 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 383 383 Size: 3467 AlwNI CAG_nnn'CTG Cuts at: 772 2339 772 Size: 1567 1900 ApaLI G'TGCA_C Cuts at: 2237 2237 Size: 3467 ApoI r'AATT_y Cuts at: 471 2820 3076 471 Size: 2349 256 862 Fragments arranged by size: 2349 862 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3467 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3467 AvaI C'yCGr_G Cuts at: 487 487 Size: 3467 BamHI G'GATC_C Cuts at: 492 492 Size: 3467 BanI G'GyrC_C Cuts at: 132 483 897 1145 1180 132 Size: 351 414 248 35 2419 Fragments arranged by size: 2419 414 351 248 35 BanII G_rGCy'C Cuts at: 325 481 1511 325 Size: 156 1030 2281 Fragments arranged by size: 2281 1030 156 BbeI G_GCGC'C Cuts at: 901 1149 901 Size: 248 3219 BbsI GAAGACnn'nnnn_ Cuts at: 150 186 460 150 Size: 36 274 3157 Fragments arranged by size: 3157 274 36 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 90 Size: 34 3433 BciVI GTATCCnnnnn_n' Cuts at: 1365 2126 3467 1365 Size: 761 1341 1365 Fragments arranged by size: 1365 1341 761 BclI T'GATC_A Cuts at: 987 987 Size: 3467 BglI GCCn_nnn'nGGC Cuts at: 263 263 Size: 3467 BglII A'GATC_T Cuts at: 982 3019 982 Size: 2037 1430 BlpI GC'TnA_GC Cuts at: 579 579 Size: 3467 Bme1580I G_kGCm'C Cuts at: 241 1092 1185 2241 241 Size: 851 93 1056 1467 Fragments arranged by size: 1467 1056 851 93 BmgBI CAC'GTC Cuts at: 354 354 Size: 3467 BmrI ACTGGGnnnn_n' Cuts at: 679 760 1095 3239 679 Size: 81 335 2144 907 Fragments arranged by size: 2144 907 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1834 1834 Size: 3467 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 419 419 Size: 3467 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 643 1522 2014 2312 643 Size: 879 492 298 1798 Fragments arranged by size: 1798 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3171 3171 Size: 3467 BsaAI yAC'GTr Cuts at: 820 820 Size: 3467 BsaBI GATnn'nnATC Cuts at: 6 981 3018 6 Size: 975 2037 455 Fragments arranged by size: 2037 975 455 BsaHI Gr'CG_yC Cuts at: 191 898 1146 1848 3344 191 Size: 707 248 702 1496 314 Fragments arranged by size: 1496 707 702 314 248 BsaWI w'CCGG_w Cuts at: 656 1177 1855 2129 2276 2687 656 Size: 521 678 274 147 411 1436 Fragments arranged by size: 1436 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 307 337 417 447 307 Size: 30 80 30 3327 Fragments arranged by size: 3327 80 30 30 BseYI C'CCAG_C Cuts at: 892 1650 2227 2723 892 Size: 758 577 496 1636 Fragments arranged by size: 1636 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 283 283 Size: 3467 BsiEI CG_ry'CG Cuts at: 104 1009 1055 2263 104 Size: 905 46 1208 1308 Fragments arranged by size: 1308 1208 905 46 BsiHKAI G_wGCw'C Cuts at: 481 1259 1449 2241 481 Size: 778 190 792 1707 Fragments arranged by size: 1707 792 778 190 Bsp1286I G_dGCh'C Cuts at: 241 325 481 1092 1185 1259 1449 1511 2241 Size: 84 156 611 93 74 190 62 730 Cuts at: 2241 241 Size: 1467 Fragments arranged by size: 1467 730 611 190 156 93 84 74 62 BspEI T'CCGG_A Cuts at: 2687 2687 Size: 3467 BspHI T'CATG_A Cuts at: 2643 2643 Size: 3467 BspMI ACCTGCnnnn'nnnn_ Cuts at: 305 450 517 731 1033 1414 305 Size: 145 67 214 302 381 2358 Fragments arranged by size: 2358 381 302 214 145 67 BsrBI CCG'CTC Cuts at: 50 1759 1813 3297 3462 50 Size: 1709 54 1484 165 55 Fragments arranged by size: 1709 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1379 1379 Size: 3467 BsrFI r'CCGG_y Cuts at: 1465 1646 1855 1465 Size: 181 209 3077 Fragments arranged by size: 3077 209 181 BsrGI T'GTAC_A Cuts at: 332 332 Size: 3467 BssSI C'ACGA_G Cuts at: 1738 2096 1738 Size: 358 3109 BstAPI GCAn_nnn'nTGC Cuts at: 844 844 Size: 3467 BstBI TT'CG_AA Cuts at: 1828 1828 Size: 3467 BstYI r'GATC_y Cuts at: 1 492 959 982 1317 1563 2575 2661 2673 Size: 491 467 23 335 246 1012 86 12 Cuts at: 2673 3019 1 Size: 346 449 Fragments arranged by size: 1012 491 467 449 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 224 224 Size: 3467 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1417 1598 1761 1417 Size: 181 163 3123 Fragments arranged by size: 3123 181 163 BtsI GCAGTG_nn' Cuts at: 710 710 Size: 3467 DraI TTT'AAA Cuts at: 466 466 Size: 3467 DrdI GACnn_nn'nnGTC Cuts at: 1173 2031 1173 Size: 858 2609 EaeI y'GGCC_r Cuts at: 1052 1226 1617 1644 2989 1052 Size: 174 391 27 1345 1530 Fragments arranged by size: 1530 1345 391 174 27 EagI C'GGCC_G Cuts at: 1052 1052 Size: 3467 EarI CTCTTCn'nnn_ Cuts at: 1490 1700 1490 Size: 210 3257 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1675 1985 2131 3277 1675 Size: 310 146 1146 1865 Fragments arranged by size: 1865 1146 310 146 EcoICRI GAG'CTC Cuts at: 479 479 Size: 3467 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 361 419 472 1292 1724 2471 2739 361 Size: 58 53 820 432 747 268 1089 Fragments arranged by size: 1089 820 747 432 268 58 53 EcoNI CCTnn'n_nnAGG Cuts at: 223 223 Size: 3467 EcoO109I rG'GnC_Cy Cuts at: 255 606 255 Size: 351 3116 EcoRI G'AATT_C Cuts at: 471 471 Size: 3467 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 381 1486 381 Size: 1105 2362 FspI TGC'GCA Cuts at: 1248 1248 Size: 3467 HaeII r_GCGC'y Cuts at: 901 1149 2171 3150 901 Size: 248 1022 979 1218 Fragments arranged by size: 1218 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 377 409 1309 1339 1341 1371 377 Size: 32 900 30 2 30 2473 Fragments arranged by size: 2473 900 32 30 30 2 HincII GTy'rAC Cuts at: 506 506 Size: 3467 KasI G'GCGC_C Cuts at: 897 1145 897 Size: 248 3219 KpnI G_GTAC'C Cuts at: 487 487 Size: 3467 MluI A'CGCG_T Cuts at: 2900 2900 Size: 3467 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2138 2322 2138 Size: 184 3283 MscI TGG'CCA Cuts at: 1228 1228 Size: 3467 MslI CAynn'nnrTG Cuts at: 1456 1583 1456 Size: 127 3340 MspA1I CmG'CkG Cuts at: 578 892 1252 2265 2510 578 Size: 314 360 1013 245 1535 Fragments arranged by size: 1535 1013 360 314 245 NaeI GCC'GGC Cuts at: 1648 1648 Size: 3467 NarI GG'CG_CC Cuts at: 898 1146 898 Size: 248 3219 NgoMIV G'CCGG_C Cuts at: 1646 1646 Size: 3467 NspI r_CATG'y Cuts at: 210 520 1020 1927 210 Size: 310 500 907 1750 Fragments arranged by size: 1750 907 500 310 PciI A'CATG_T Cuts at: 1923 1923 Size: 3467 PmeI GTTT'AAAC Cuts at: 466 466 Size: 3467 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2606 2638 2606 Size: 32 3435 PstI C_TGCA'G Cuts at: 131 514 1199 131 Size: 383 685 2399 Fragments arranged by size: 2399 685 383 PvuI CG_AT'CG Cuts at: 104 104 Size: 3467 PvuII CAG'CTG Cuts at: 892 1252 892 Size: 360 3107 RsrII CG'GwC_CG Cuts at: 1662 1662 Size: 3467 SacI G_AGCT'C Cuts at: 481 481 Size: 3467 SalI G'TCGA_C Cuts at: 504 504 Size: 3467 SapI GCTCTTCn'nnn_ Cuts at: 1490 1700 1490 Size: 210 3257 SbfI CC_TGCA'GG Cuts at: 514 514 Size: 3467 ScaI AGT'ACT Cuts at: 2984 2984 Size: 3467 SfcI C'TryA_G Cuts at: 33 127 510 1195 2188 2379 33 Size: 94 383 685 993 191 1121 Fragments arranged by size: 1121 993 685 383 191 94 SfoI GGC'GCC Cuts at: 899 1147 899 Size: 248 3219 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3467 SmaI CCC'GGG Cuts at: 489 489 Size: 3467 SmlI C'TyrA_G Cuts at: 622 1537 2029 2291 622 Size: 915 492 262 1798 Fragments arranged by size: 1798 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3467 SphI G_CATG'C Cuts at: 210 520 1020 210 Size: 310 500 2657 Fragments arranged by size: 2657 500 310 StyI C'CwwG_G Cuts at: 601 955 601 Size: 354 3113 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1050 1183 1642 1851 1050 Size: 133 459 209 2666 Fragments arranged by size: 2666 459 209 133 TatI w'GTAC_w Cuts at: 332 2982 332 Size: 2650 817 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 307 339 417 449 2984 3016 307 Size: 32 78 32 2535 32 758 Fragments arranged by size: 2535 758 78 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1264 1264 Size: 3467 XbaI T'CTAG_A Cuts at: 498 498 Size: 3467 XcmI CCAnnnn_n'nnnnTGG Cuts at: 962 962 Size: 3467 XmaI C'CCGG_G Cuts at: 487 487 Size: 3467 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BmtI Bpu10I BseRI BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I BtgI ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI