Restriction map for
pF1KE6677
(Linear) (Six-base) MAPSORT of: KIEE6677.seq Check: 3605 from: 1 to: 891
KIEE6677.orf
With 182 enzymes: SgfI *
October 17, 2018 19:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 369 891
Size: 369 522
Acc65I G'GTAC_C
Cuts at: 0 167 891
Size: 167 724
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 244 891
Size: 166 78 647
Fragments arranged by size:
647 166 78
AflIII A'CryG_T
Cuts at: 0 89 891
Size: 89 802
ApoI r'AATT_y
Cuts at: 0 703 843 891
Size: 703 140 48
Fragments arranged by size:
703 140 48
AvaI C'yCGr_G
Cuts at: 0 38 499 891
Size: 38 461 392
Fragments arranged by size:
461 392 38
BanI G'GyrC_C
Cuts at: 0 167 891
Size: 167 724
BanII G_rGCy'C
Cuts at: 0 334 677 891
Size: 334 343 214
Fragments arranged by size:
343 334 214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 891
Size: 496 395
Bpu10I CC'TnA_GC
Cuts at: 0 277 891
Size: 277 614
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 891
Size: 229 662
BsaI GGTCTCn'nnnn_
Cuts at: 0 559 891
Size: 559 332
BsaAI yAC'GTr
Cuts at: 0 213 891
Size: 213 678
BsaHI Gr'CG_yC
Cuts at: 0 407 891
Size: 407 484
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 133 208 265 279 517 891
Size: 52 81 75 57 14 238 374
Fragments arranged by size:
374 238 81 75 57 52 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 355 891
Size: 84 271 536
Fragments arranged by size:
536 271 84
BsiHKAI G_wGCw'C
Cuts at: 0 62 677 891
Size: 62 615 214
Fragments arranged by size:
615 214 62
BsmI GAATG_Cn'
Cuts at: 0 405 891
Size: 405 486
Bsp1286I G_dGCh'C
Cuts at: 0 62 334 677 891
Size: 62 272 343 214
Fragments arranged by size:
343 272 214 62
BspHI T'CATG_A
Cuts at: 0 743 891
Size: 743 148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 369 891
Size: 369 522
BsrGI T'GTAC_A
Cuts at: 0 86 891
Size: 86 805
BstAPI GCAn_nnn'nTGC
Cuts at: 0 837 891
Size: 837 54
BstYI r'GATC_y
Cuts at: 0 635 891
Size: 635 256
BtgI C'CryG_G
Cuts at: 0 152 891
Size: 152 739
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 317 891
Size: 317 574
BtsI GCAGTG_nn'
Cuts at: 0 130 440 891
Size: 130 310 451
Fragments arranged by size:
451 310 130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 797 832 891
Size: 797 35 59
Fragments arranged by size:
797 59 35
EaeI y'GGCC_r
Cuts at: 0 416 773 891
Size: 416 357 118
Fragments arranged by size:
416 357 118
EcoICRI GAG'CTC
Cuts at: 0 675 891
Size: 675 216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 244 496 891
Size: 166 78 252 395
Fragments arranged by size:
395 252 166 78
EcoRI G'AATT_C
Cuts at: 0 703 891
Size: 703 188
KpnI G_GTAC'C
Cuts at: 0 171 891
Size: 171 720
MscI TGG'CCA
Cuts at: 0 418 775 891
Size: 418 357 116
Fragments arranged by size:
418 357 116
MslI CAynn'nnrTG
Cuts at: 0 698 748 891
Size: 698 50 143
Fragments arranged by size:
698 143 50
MspA1I CmG'CkG
Cuts at: 0 126 474 891
Size: 126 348 417
Fragments arranged by size:
417 348 126
NspI r_CATG'y
Cuts at: 0 93 891
Size: 93 798
PasI CC'CwG_GG
Cuts at: 0 137 891
Size: 137 754
PciI A'CATG_T
Cuts at: 0 89 891
Size: 89 802
PstI C_TGCA'G
Cuts at: 0 260 585 891
Size: 260 325 306
Fragments arranged by size:
325 306 260
PvuII CAG'CTG
Cuts at: 0 126 474 891
Size: 126 348 417
Fragments arranged by size:
417 348 126
SacI G_AGCT'C
Cuts at: 0 677 891
Size: 677 214
SfcI C'TryA_G
Cuts at: 0 256 457 581 891
Size: 256 201 124 310
Fragments arranged by size:
310 256 201 124
SmaI CCC'GGG
Cuts at: 0 40 891
Size: 40 851
SmlI C'TyrA_G
Cuts at: 0 244 891
Size: 244 647
StuI AGG'CCT
Cuts at: 0 852 891
Size: 852 39
StyI C'CwwG_G
Cuts at: 0 560 891
Size: 560 331
TatI w'GTAC_w
Cuts at: 0 86 891
Size: 86 805
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 478 747 891
Size: 478 269 144
Fragments arranged by size:
478 269 144
XmaI C'CCGG_G
Cuts at: 0 38 891
Size: 38 853
Enzymes that do cut:
AarI Acc65I AcuI AflIII ApoI AvaI BanI BanII
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BseRI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrGI BstAPI BstYI
BtgI BtgZI BtsI CspCI EaeI EcoICRI Eco57MI EcoRI
KpnI MscI MslI MspA1I NspI PasI PciI PstI
PvuII SacI SfcI SmaI SmlI StuI StyI TatI
XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BsaBI BsaWI BsaXI BseYI BsiEI BsiWI
BsmBI BspEI BsrBI BsrDI BsrFI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I ClaI DraI DraIII DrdI
EagI EarI EciI EcoNI EcoO109I EcoRV FalI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TstI Tth111I XbaI XhoI
XmnI ZraI