Restriction map for pF1KE6679
 (Linear) (Six-base) MAPSORT of: KIEE6679.seq  Check: 4555  from: 1  to: 984



KIEE6679.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     240     910     984 
   Size:        240     670      74

  Fragments arranged by size: 

                 670     240      74
 
AccI GT'mk_AC

Cuts at:      0     278     984 
   Size:        278     706
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     267     344     984 
   Size:        267      77     640

  Fragments arranged by size: 

                 640     267      77
 
ApaI G_GGCC'C

Cuts at:      0     844     898     907     984 
   Size:        844      54       9      77

  Fragments arranged by size: 

                 844      77      54       9
 
ApoI r'AATT_y

Cuts at:      0      17     300     510     984 
   Size:         17     283     210     474

  Fragments arranged by size: 

                 474     283     210      17
 
AseI AT'TA_AT

Cuts at:      0     437     984 
   Size:        437     547
 
AvaI C'yCGr_G

Cuts at:      0     810     829     900     909     984 
   Size:        810      19      71       9      75

  Fragments arranged by size: 

                 810      75      71      19       9
 
BanII G_rGCy'C

Cuts at:      0     844     898     907     945     984 
   Size:        844      54       9      38      39

  Fragments arranged by size: 

                 844      54      39      38       9
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      10     984 
   Size:         10     974
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     488     522     984 
   Size:        488      34     462

  Fragments arranged by size: 

                 488     462      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     902     984 
   Size:        902      82
 
Bme1580I G_kGCm'C

Cuts at:      0     844     898     907     984 
   Size:        844      54       9      77

  Fragments arranged by size: 

                 844      77      54       9
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     305     528     984 
   Size:        305     223     456

  Fragments arranged by size: 

                 456     305     223
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     869     929     984 
   Size:        869      60      55

  Fragments arranged by size: 

                 869      60      55
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     665     984 
   Size:        665     319
 
BsaBI GATnn'nnATC

Cuts at:      0     715     984 
   Size:        715     269
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     579     984 
   Size:        579     405
 
BseYI C'CCAG_C

Cuts at:      0     657     781     984 
   Size:        657     124     203

  Fragments arranged by size: 

                 657     203     124
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     984 
   Size:         60     924
 
BsiHKAI G_wGCw'C

Cuts at:      0     945     984 
   Size:        945      39
 
Bsp1286I G_dGCh'C

Cuts at:      0     844     898     907     945     984 
   Size:        844      54       9      38      39

  Fragments arranged by size: 

                 844      54      39      38       9
 
BspHI T'CATG_A

Cuts at:      0     548     984 
   Size:        548     436
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      16     240     910     984 
   Size:         16     224     670      74

  Fragments arranged by size: 

                 670     224      74      16
 
BsrDI GCAATG_nn'

Cuts at:      0     244     984 
   Size:        244     740
 
BstBI TT'CG_AA

Cuts at:      0     351     508     984 
   Size:        351     157     476

  Fragments arranged by size: 

                 476     351     157
 
BstEII G'GTnAC_C

Cuts at:      0     877     984 
   Size:        877     107
 
Bsu36I CC'TnA_GG

Cuts at:      0     890     984 
   Size:        890      94
 
ClaI AT'CG_AT

Cuts at:      0     863     984 
   Size:        863     121
 
EaeI y'GGCC_r

Cuts at:      0     147     441     984 
   Size:        147     294     543

  Fragments arranged by size: 

                 543     294     147
 
EarI CTCTTCn'nnn_

Cuts at:      0     207     984 
   Size:        207     777
 
EcoICRI GAG'CTC

Cuts at:      0     943     984 
   Size:        943      41
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     267     344     869     929     984 
   Size:        267      77     525      60      55

  Fragments arranged by size: 

                 525     267      77      60      55
 
EcoO109I rG'GnC_Cy

Cuts at:      0     805     823     841     886     894     895     904     913 
   Size:        805      18      18      45       8       1       9       9

Cuts at:    913     984 
   Size:         71

  Fragments arranged by size: 

                 805      71      45      18      18       9       9       8
                   1
 
HaeII r_GCGC'y

Cuts at:      0     971     984 
   Size:        971      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      80     112     163     195     984 
   Size:         80      32      51      32     789

  Fragments arranged by size: 

                 789      80      51      32      32
 
HincII GTy'rAC

Cuts at:      0     588     984 
   Size:        588     396
 
MfeI C'AATT_G

Cuts at:      0     632     984 
   Size:        632     352
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     316     984 
   Size:        316     668
 
MscI TGG'CCA

Cuts at:      0     149     443     984 
   Size:        149     294     541

  Fragments arranged by size: 

                 541     294     149
 
MspA1I CmG'CkG

Cuts at:      0      70     984 
   Size:         70     914
 
PasI CC'CwG_GG

Cuts at:      0      56     984 
   Size:         56     928
 
PfoI T'CCnGG_A

Cuts at:      0      47     984 
   Size:         47     937
 
PpuMI rG'GwC_Cy

Cuts at:      0     805     823     886     913     984 
   Size:        805      18      63      27      71

  Fragments arranged by size: 

                 805      71      63      27      18
 
PshAI GACnn'nnGTC

Cuts at:      0     884     984 
   Size:        884     100
 
PspOMI G'GGCC_C

Cuts at:      0     840     894     903     984 
   Size:        840      54       9      81

  Fragments arranged by size: 

                 840      81      54       9
 
PstI C_TGCA'G

Cuts at:      0      69     921     984 
   Size:         69     852      63

  Fragments arranged by size: 

                 852      69      63
 
PvuII CAG'CTG

Cuts at:      0      70     984 
   Size:         70     914
 
SacI G_AGCT'C

Cuts at:      0     945     984 
   Size:        945      39
 
SapI GCTCTTCn'nnn_

Cuts at:      0     207     984 
   Size:        207     777
 
SbfI CC_TGCA'GG

Cuts at:      0     921     984 
   Size:        921      63
 
SfcI C'TryA_G

Cuts at:      0      65     917     984 
   Size:         65     852      67

  Fragments arranged by size: 

                 852      67      65
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     902     984 
   Size:        902      82
 
SmaI CCC'GGG

Cuts at:      0     812     831     902     911     984 
   Size:        812      19      71       9      73

  Fragments arranged by size: 

                 812      73      71      19       9
 
SmlI C'TyrA_G

Cuts at:      0     680     984 
   Size:        680     304
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     896     984 
   Size:        896      88
 
XbaI T'CTAG_A

Cuts at:      0     208     984 
   Size:        208     776
 
XmaI C'CCGG_G

Cuts at:      0     810     829     900     909     984 
   Size:        810      19      71       9      75

  Fragments arranged by size: 

                 810      75      71      19       9
 

 Enzymes that do cut:

     AarI     AccI     AcuI     ApaI     ApoI     AseI     AvaI    BanII
     BbsI     BcgI     BglI Bme1580I     BmrI     BpmI    BpuEI    BsaBI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI
    BstBI   BstEII   Bsu36I     ClaI     EaeI     EarI  EcoICRI  Eco57MI
 EcoO109I    HaeII    Hin4I   HincII     MfeI     MmeI     MscI   MspA1I
     PasI     PfoI    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI
     SapI     SbfI     SfcI     SfiI     SmaI     SmlI    TaqII     XbaI
     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI    ApaLI     AscI    AsiSI    AvrII
     BaeI    BamHI     BanI     BbeI    BbvCI    BciVI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI
     EagI     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     HpaI     KasI     KpnI     MluI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PmeI     PmlI     PpiI     PsiI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     ScaI
    SexAI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I     XcmI
     XhoI     XmnI     ZraI
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