################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BspEI BspHI BspLU11I BspMI BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CpoI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FseI FspI HaeII HincII HindII HindIII HpaI KasI Ksp632I KspI MaeII MamI MfeI MflI MluI MroI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StyI SwaI Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 674 GGTACC G^GTACC AccI 109 GTAGAC GT^[AC][GT]AC AccII 753 CGCG CG^CG AcyI 781 GACGCC G[GA]^CG[CT]C Asp718I 674 GGTACC G^GTACC BalI 238 TGGCCA TGG^CCA BanII 789 GAGCCC G[GA]GC[CT]^C BlpI 823 GCTGAGC GC^T[ACGT]AGC Bpu1102I 823 GCTGAGC GC^T[ACGT]AGC BsaHI 781 GACGCC G[GA]^CG[CT]C BsgI 715 CTGCAC GTGCAG(16/14) Bsp1407I 571 TGTACA T^GTACA BspDI 577 ATCGAT AT^CGAT BsrDI 179 GCAATG GCAATG(2/0) BsrGI 571 TGTACA T^GTACA BstUI 753 CGCG CG^CG CelII 823 GCTGAGC GC^T[ACGT]AGC ClaI 577 ATCGAT AT^CGAT DraI 517 TTTAAA TTT^AAA EcoNI 813 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 473 GATATC GAT^ATC FauI 765 GCGGG CCCGC(4/6) HgaI 781 GACGC GACGC(5/10) Hin1I 781 GACGCC G[GA]^CG[CT]C KpnI 674 GGTACC GGTAC^C MboII 942 GAAGA GAAGA(8/7) MluNI 238 TGGCCA TGG^CCA MscI 238 TGGCCA TGG^CCA MvnI 753 CGCG CG^CG NaeI 724 GCCGGC GCC^GGC NgoMIV 724 GCCGGC G^CCGGC PflMI 241 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SmaI 949 CCCGGG CCC^GGG SspBI 571 TGTACA T^GTACA TaqI 578 TCGA T^CGA TfiI 945 GATTC G^A[AT]TC Tsp45I 184 GTCAC ^GT[GC]AC TthHB8I 578 TCGA T^CGA Van91I 241 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 949 CCCGGG C^CCGGG XmaI 949 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AspHI 76 GAGCAC G[AT]GC[AT]^C 805 GTGCTC AvaII 270 GGTCC G^G[AT]CC 415 GGTCC BanI 251 GGCACC G^G[CT][GA]CC 674 GGTACC BclI 842 TGATCA T^GATCA 926 TGATCA BglI 204 GCCTGGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 724 GCCGGCAAGGC BpmI 73 CTGGAG CTGGAG(16/14) 817 CTGGAG BsiHKAI 76 GAGCAC G[AT]GC[AT]^C 805 GTGCTC BsmAI 364 GAGAC GTCTC(1/5) 690 GAGAC BsmFI 314 GTCCC GGGAC(10/14) 888 GTCCC BsmI 170 GCATTC GAATGC(1/-1) 527 GCATTC BsrFI 587 ACCGGC [GA]^CCGG[CT] 724 GCCGGC BstAPI 593 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 798 GCAGCTAGTGC Cfr10I 587 ACCGGC [GA]^CCGG[CT] 724 GCCGGC DdeI 787 CTGAG C^T[ACGT]AG 824 CTGAG FbaI 842 TGATCA T^GATCA 926 TGATCA MseI 29 TTAA T^TAA 518 TTAA MslI 289 CATCTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 879 CATGATGATG MspA1I 712 CAGCTG C[AC]G^C[GT]G 930 CAGCTG PleI 312 GAGTC GAGTC(4/5) 485 GAGTC PstI 51 CTGCAG CTGCA^G 453 CTGCAG PvuII 712 CAGCTG CAG^CTG 930 CAGCTG SfaNI 433 GATGC GCATC(5/9) 466 GCATC Tru9I 29 TTAA T^TAA 518 TTAA Tsp509I 31 AATT ^AATT 163 AATT XcmI 62 CCATCTTACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 226 CCAAATGTGTTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AfaI AluI AvaI BbvI BcnI BfaI BmyI BsaJI BsiYI BslI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DpnII EaeI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfcI StuI TseI TspRI