(Circular) (Six-base) MAPSORT of: pF1KE2464.seq Check: 6305 from: 1 to: 5402 pF1KE2464 5402 bp With 182 enzymes: SgfI * March 1, 2013 10:20 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2073 2073 Size: 5402 AccI GT'mk_AC Cuts at: 2440 2440 Size: 5402 Acc65I G'GTAC_C Cuts at: 2418 2418 Size: 5402 AclI AA'CG_TT Cuts at: 5242 5242 Size: 5402 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 179 340 478 859 1093 1235 1462 1525 1738 Size: 161 138 381 234 142 227 63 213 Cuts at: 1738 1921 3227 3659 4406 4674 179 Size: 183 1306 432 747 268 907 Fragments arranged by size: 1306 907 747 432 381 268 234 227 213 183 161 142 138 63 AflIII A'CryG_T Cuts at: 1179 2327 2754 3858 4835 1179 Size: 1148 427 1104 977 1746 Fragments arranged by size: 1746 1148 1104 977 427 AgeI A'CCGG_T Cuts at: 3790 3790 Size: 5402 AlwNI CAG_nnn'CTG Cuts at: 687 916 1503 2707 4274 687 Size: 229 587 1204 1567 1815 Fragments arranged by size: 1815 1567 1204 587 229 ApaLI G'TGCA_C Cuts at: 4172 4172 Size: 5402 ApoI r'AATT_y Cuts at: 2406 4755 5011 2406 Size: 2349 256 2797 Fragments arranged by size: 2797 2349 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5402 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5402 AvaI C'yCGr_G Cuts at: 1489 2422 1489 Size: 933 4469 BamHI G'GATC_C Cuts at: 2427 2427 Size: 5402 BanI G'GyrC_C Cuts at: 2418 2832 3080 3115 2418 Size: 414 248 35 4705 Fragments arranged by size: 4705 414 248 35 BanII G_rGCy'C Cuts at: 872 1134 2416 3446 872 Size: 262 1282 1030 2828 Fragments arranged by size: 2828 1282 1030 262 BbeI G_GCGC'C Cuts at: 2836 3084 2836 Size: 248 5154 BbsI GAAGACnn'nnnn_ Cuts at: 1370 1570 1370 Size: 200 5202 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1147 1181 1803 1837 1147 Size: 34 622 34 4712 Fragments arranged by size: 4712 622 34 34 BciVI GTATCCnnnnn_n' Cuts at: 591 3300 4061 5402 591 Size: 2709 761 1341 591 Fragments arranged by size: 2709 1341 761 591 BclI T'GATC_A Cuts at: 2922 2922 Size: 5402 BglII A'GATC_T Cuts at: 1320 2917 4954 1320 Size: 1597 2037 1768 Fragments arranged by size: 2037 1768 1597 BlpI GC'TnA_GC Cuts at: 688 1169 2161 2514 688 Size: 481 992 353 3576 Fragments arranged by size: 3576 992 481 353 Bme1580I G_kGCm'C Cuts at: 1944 3027 3120 4176 1944 Size: 1083 93 1056 3170 Fragments arranged by size: 3170 1083 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 2614 2695 3030 5174 2614 Size: 81 335 2144 2842 Fragments arranged by size: 2842 2144 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 828 3769 828 Size: 2941 2461 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 196 394 415 1039 1260 1366 1447 1864 1936 Size: 198 21 624 221 106 81 417 72 Cuts at: 1936 1948 2032 2278 196 Size: 12 84 246 3320 Fragments arranged by size: 3320 624 417 246 221 198 106 84 81 72 21 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 884 1303 2170 2578 3457 3949 4247 884 Size: 419 867 408 879 492 298 2039 Fragments arranged by size: 2039 879 867 492 419 408 298 BsaI GGTCTCn'nnnn_ Cuts at: 213 697 716 1088 1271 2146 5106 213 Size: 484 19 372 183 875 2960 509 Fragments arranged by size: 2960 875 509 484 372 183 19 BsaAI yAC'GTr Cuts at: 1182 2755 1182 Size: 1573 3829 BsaBI GATnn'nnATC Cuts at: 6 2916 4953 6 Size: 2910 2037 455 Fragments arranged by size: 2910 2037 455 BsaHI Gr'CG_yC Cuts at: 2833 3081 3783 5279 2833 Size: 248 702 1496 2956 Fragments arranged by size: 2956 1496 702 248 BsaWI w'CCGG_w Cuts at: 2591 3112 3790 4064 4211 4622 2591 Size: 521 678 274 147 411 3371 Fragments arranged by size: 3371 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 508 538 2095 2125 2352 2382 508 Size: 30 1557 30 227 30 3528 Fragments arranged by size: 3528 1557 227 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 412 487 715 1174 1257 1363 1996 2029 2091 Size: 75 228 459 83 106 633 33 62 Cuts at: 2091 2378 412 Size: 287 3436 Fragments arranged by size: 3436 633 459 287 228 106 83 75 62 33 BseYI C'CCAG_C Cuts at: 1595 2000 2156 2827 3585 4162 4658 1595 Size: 405 156 671 758 577 496 2339 Fragments arranged by size: 2339 758 671 577 496 405 156 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 621 621 Size: 5402 BsiEI CG_ry'CG Cuts at: 104 2944 2990 4198 104 Size: 2840 46 1208 1308 Fragments arranged by size: 2840 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 431 694 786 872 2105 2275 2416 3194 3384 Size: 263 92 86 1233 170 141 778 190 Cuts at: 3384 4176 431 Size: 792 1657 Fragments arranged by size: 1657 1233 792 778 263 190 170 141 92 86 BsmI GAATG_Cn' Cuts at: 309 1417 309 Size: 1108 4294 Bsp1286I G_dGCh'C Cuts at: 431 694 786 872 1134 1944 2105 2275 2416 Size: 263 92 86 262 810 161 170 141 Cuts at: 2416 3027 3120 3194 3384 3446 4176 431 Size: 611 93 74 190 62 730 1657 Fragments arranged by size: 1657 810 730 611 263 262 190 170 161 141 93 92 86 74 62 BspEI T'CCGG_A Cuts at: 4622 4622 Size: 5402 BspHI T'CATG_A Cuts at: 4578 4578 Size: 5402 BspMI ACCTGCnnnn'nnnn_ Cuts at: 570 643 1703 1817 2073 2452 2666 2968 3349 Size: 73 1060 114 256 379 214 302 381 Cuts at: 3349 570 Size: 2623 Fragments arranged by size: 2623 1060 381 379 302 256 214 114 73 BsrBI CCG'CTC Cuts at: 50 1773 2378 3694 3748 5232 5397 50 Size: 1723 605 1316 54 1484 165 55 Fragments arranged by size: 1723 1484 1316 605 165 55 54 BsrDI GCAATG_nn' Cuts at: 168 3314 168 Size: 3146 2256 BsrFI r'CCGG_y Cuts at: 2231 3400 3581 3790 2231 Size: 1169 181 209 3843 Fragments arranged by size: 3843 1169 209 181 BssHII G'CGCG_C Cuts at: 823 823 Size: 5402 BssSI C'ACGA_G Cuts at: 430 3673 4031 430 Size: 3243 358 1801 Fragments arranged by size: 3243 1801 358 BstAPI GCAn_nnn'nTGC Cuts at: 2779 2779 Size: 5402 BstBI TT'CG_AA Cuts at: 3763 3763 Size: 5402 BstXI CCAn_nnnn'nTGG Cuts at: 543 543 Size: 5402 BstYI r'GATC_y Cuts at: 1 1200 1320 1625 2427 2894 2917 3252 3498 Size: 1199 120 305 802 467 23 335 246 Cuts at: 3498 4510 4596 4608 4954 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1199 1012 802 467 449 346 335 305 246 120 86 23 12 Bsu36I CC'TnA_GG Cuts at: 730 730 Size: 5402 BtgI C'CryG_G Cuts at: 932 932 Size: 5402 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3352 3533 3696 3352 Size: 181 163 5058 Fragments arranged by size: 5058 181 163 BtsI GCAGTG_nn' Cuts at: 741 784 2645 741 Size: 43 1861 3498 Fragments arranged by size: 3498 1861 43 DraI TTT'AAA Cuts at: 2401 2401 Size: 5402 DrdI GACnn_nn'nnGTC Cuts at: 1394 3108 3966 1394 Size: 1714 858 2830 Fragments arranged by size: 2830 1714 858 EaeI y'GGCC_r Cuts at: 359 2987 3161 3552 3579 4924 359 Size: 2628 174 391 27 1345 837 Fragments arranged by size: 2628 1345 837 391 174 27 EagI C'GGCC_G Cuts at: 2987 2987 Size: 5402 EarI CTCTTCn'nnn_ Cuts at: 383 860 1147 3425 3635 383 Size: 477 287 2278 210 2150 Fragments arranged by size: 2278 2150 477 287 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 150 565 817 2211 3610 3920 4066 5212 150 Size: 415 252 1394 1399 310 146 1146 340 Fragments arranged by size: 1399 1394 1146 415 340 310 252 146 EcoICRI GAG'CTC Cuts at: 870 2414 870 Size: 1544 3858 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 179 196 340 394 415 478 859 1039 1093 Size: 17 144 54 21 63 381 180 54 Cuts at: 1093 1235 1260 1366 1447 1462 1525 1738 1864 Size: 142 25 106 81 15 63 213 126 Cuts at: 1864 1921 1936 1948 2032 2278 3227 3659 4406 Size: 57 15 12 84 246 949 432 747 Cuts at: 4406 4674 179 Size: 268 907 Fragments arranged by size: 949 907 747 432 381 268 246 213 180 144 142 126 106 84 81 63 63 57 54 54 25 21 17 15 15 12 EcoNI CCTnn'n_nnAGG Cuts at: 511 511 Size: 5402 EcoO109I rG'GnC_Cy Cuts at: 2036 2541 2036 Size: 505 4897 EcoRI G'AATT_C Cuts at: 2406 2406 Size: 5402 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 889 1467 3421 889 Size: 578 1954 2870 Fragments arranged by size: 2870 1954 578 FspI TGC'GCA Cuts at: 1813 3183 1813 Size: 1370 4032 HaeII r_GCGC'y Cuts at: 354 2836 3084 4106 5085 354 Size: 2482 248 1022 979 671 Fragments arranged by size: 2482 1022 979 671 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 235 267 279 311 999 1031 2352 2384 3244 Size: 32 12 32 688 32 1321 32 860 Cuts at: 3244 3274 3276 3306 235 Size: 30 2 30 2331 Fragments arranged by size: 2331 1321 860 688 32 32 32 32 30 30 12 2 HincII GTy'rAC Cuts at: 2441 2441 Size: 5402 HindIII A'AGCT_T Cuts at: 1217 1217 Size: 5402 KasI G'GCGC_C Cuts at: 2832 3080 2832 Size: 248 5154 KpnI G_GTAC'C Cuts at: 2422 2422 Size: 5402 MluI A'CGCG_T Cuts at: 4835 4835 Size: 5402 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2033 4073 4257 2033 Size: 2040 184 3178 Fragments arranged by size: 3178 2040 184 MscI TGG'CCA Cuts at: 361 3163 361 Size: 2802 2600 MslI CAynn'nnrTG Cuts at: 2028 3391 3518 2028 Size: 1363 127 3912 Fragments arranged by size: 3912 1363 127 MspA1I CmG'CkG Cuts at: 1028 1071 1503 1686 1893 2004 2160 2190 2513 Size: 43 432 183 207 111 156 30 323 Cuts at: 2513 2827 3187 4200 4445 1028 Size: 314 360 1013 245 1985 Fragments arranged by size: 1985 1013 432 360 323 314 245 207 183 156 111 43 30 NaeI GCC'GGC Cuts at: 2233 3583 2233 Size: 1350 4052 NarI GG'CG_CC Cuts at: 2833 3081 2833 Size: 248 5154 NcoI C'CATG_G Cuts at: 932 932 Size: 5402 NgoMIV G'CCGG_C Cuts at: 2231 3581 2231 Size: 1350 4052 NspI r_CATG'y Cuts at: 1005 2289 2455 2955 3862 1005 Size: 1284 166 500 907 2545 Fragments arranged by size: 2545 1284 907 500 166 PciI A'CATG_T Cuts at: 3858 3858 Size: 5402 PflMI CCAn_nnn'nTGG Cuts at: 414 414 Size: 5402 PfoI T'CCnGG_A Cuts at: 371 1237 2141 371 Size: 866 904 3632 Fragments arranged by size: 3632 904 866 PmeI GTTT'AAAC Cuts at: 2401 2401 Size: 5402 PmlI CAC'GTG Cuts at: 1182 1182 Size: 5402 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4541 4573 4541 Size: 32 5370 PpuMI rG'GwC_Cy Cuts at: 2036 2036 Size: 5402 PshAI GACnn'nnGTC Cuts at: 254 254 Size: 5402 PspXI vC'TCGA_Gb Cuts at: 1489 1489 Size: 5402 PstI C_TGCA'G Cuts at: 683 1700 1763 2449 3134 683 Size: 1017 63 686 685 2951 Fragments arranged by size: 2951 1017 686 685 63 PvuI CG_AT'CG Cuts at: 104 104 Size: 5402 PvuII CAG'CTG Cuts at: 1071 1503 1686 1893 2004 2160 2190 2827 3187 Size: 432 183 207 111 156 30 637 360 Cuts at: 3187 1071 Size: 3286 Fragments arranged by size: 3286 637 432 360 207 183 156 111 30 RsrII CG'GwC_CG Cuts at: 2214 3597 2214 Size: 1383 4019 SacI G_AGCT'C Cuts at: 872 2416 872 Size: 1544 3858 SalI G'TCGA_C Cuts at: 2439 2439 Size: 5402 SanDI GG'GwC_CC Cuts at: 2036 2036 Size: 5402 SapI GCTCTTCn'nnn_ Cuts at: 383 860 1147 3425 3635 383 Size: 477 287 2278 210 2150 Fragments arranged by size: 2278 2150 477 287 210 SbfI CC_TGCA'GG Cuts at: 2449 2449 Size: 5402 ScaI AGT'ACT Cuts at: 4919 4919 Size: 5402 SfcI C'TryA_G Cuts at: 33 236 679 1696 1759 1960 2445 3130 4123 Size: 203 443 1017 63 201 485 685 993 Cuts at: 4123 4314 33 Size: 191 1121 Fragments arranged by size: 1121 1017 993 685 485 443 203 201 191 63 SfoI GGC'GCC Cuts at: 2834 3082 2834 Size: 248 5154 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5402 SmaI CCC'GGG Cuts at: 2424 2424 Size: 5402 SmlI C'TyrA_G Cuts at: 899 1282 1489 2149 2557 3472 3964 4226 899 Size: 383 207 660 408 915 492 262 2075 Fragments arranged by size: 2075 915 660 492 408 383 262 207 SpeI A'CTAG_T Cuts at: 67 67 Size: 5402 SphI G_CATG'C Cuts at: 1005 2289 2455 2955 1005 Size: 1284 166 500 3452 Fragments arranged by size: 3452 1284 500 166 StuI AGG'CCT Cuts at: 1510 1510 Size: 5402 StyI C'CwwG_G Cuts at: 932 2096 2114 2536 2890 932 Size: 1164 18 422 354 3444 Fragments arranged by size: 3444 1164 422 354 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 959 1112 2985 3118 3577 3786 959 Size: 153 1873 133 459 209 2575 Fragments arranged by size: 2575 1873 459 209 153 133 TatI w'GTAC_w Cuts at: 4917 4917 Size: 5402 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 508 540 2095 2127 4919 4951 508 Size: 32 1555 32 2792 32 959 Fragments arranged by size: 2792 1555 959 32 32 32 Tth111I GACn'n_nGTC Cuts at: 3199 3199 Size: 5402 XbaI T'CTAG_A Cuts at: 2433 2433 Size: 5402 XcmI CCAnnnn_n'nnnnTGG Cuts at: 369 582 2897 369 Size: 213 2315 2874 Fragments arranged by size: 2874 2315 213 XhoI C'TCGA_G Cuts at: 1489 1489 Size: 5402 XmaI C'CCGG_G Cuts at: 2422 2422 Size: 5402 XmnI GAAnn'nnTTC Cuts at: 481 481 Size: 5402 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspXI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsiWI BsmBI BsrGI BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PsiI PspOMI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI ZraI