(Circular) (Six-base) MAPSORT of: pF1KE2641.seq Check: 4172 from: 1 to: 4430 pF1KE2641 4430 bp With 182 enzymes: SgfI * October 5, 2016 15:26 .. AccI GT'mk_AC Cuts at: 1468 1468 Size: 4430 Acc65I G'GTAC_C Cuts at: 570 1446 570 Size: 876 3554 AclI AA'CG_TT Cuts at: 4270 4270 Size: 4430 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 560 781 919 1439 2255 2687 3434 3702 560 Size: 221 138 520 816 432 747 268 1288 Fragments arranged by size: 1288 816 747 520 432 268 221 138 AfeI AGC'GCT Cuts at: 916 916 Size: 4430 AflIII A'CryG_T Cuts at: 1020 1275 1782 2886 3863 1020 Size: 255 507 1104 977 1587 Fragments arranged by size: 1587 1104 977 507 255 AgeI A'CCGG_T Cuts at: 2818 2818 Size: 4430 AlwNI CAG_nnn'CTG Cuts at: 1735 3302 1735 Size: 1567 2863 ApaLI G'TGCA_C Cuts at: 3200 3200 Size: 4430 ApoI r'AATT_y Cuts at: 448 1095 1434 3783 4039 448 Size: 647 339 2349 256 839 Fragments arranged by size: 2349 839 647 339 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4430 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4430 AvaI C'yCGr_G Cuts at: 1450 1450 Size: 4430 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 782 815 782 Size: 33 4397 BamHI G'GATC_C Cuts at: 1455 1455 Size: 4430 BanI G'GyrC_C Cuts at: 570 1039 1446 1860 2108 2143 570 Size: 469 407 414 248 35 2857 Fragments arranged by size: 2857 469 414 407 248 35 BanII G_rGCy'C Cuts at: 1444 2474 1444 Size: 1030 3400 BbeI G_GCGC'C Cuts at: 1864 2112 1864 Size: 248 4182 BbsI GAAGACnn'nnnn_ Cuts at: 819 819 Size: 4430 BciVI GTATCCnnnnn_n' Cuts at: 2328 3089 4430 2328 Size: 761 1341 2328 Fragments arranged by size: 2328 1341 761 BclI T'GATC_A Cuts at: 110 702 1950 110 Size: 592 1248 2590 Fragments arranged by size: 2590 1248 592 BglII A'GATC_T Cuts at: 1945 3982 1945 Size: 2037 2393 BlpI GC'TnA_GC Cuts at: 1542 1542 Size: 4430 Bme1580I G_kGCm'C Cuts at: 1042 2055 2148 3204 1042 Size: 1013 93 1056 2268 Fragments arranged by size: 2268 1056 1013 93 BmrI ACTGGGnnnn_n' Cuts at: 1642 1723 2058 4202 1642 Size: 81 335 2144 1870 Fragments arranged by size: 2144 1870 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 153 832 2797 153 Size: 679 1965 1786 Fragments arranged by size: 1965 1786 679 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 779 986 1133 1309 779 Size: 207 147 176 3900 Fragments arranged by size: 3900 207 176 147 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 127 895 1606 2485 2977 3275 127 Size: 768 711 879 492 298 1282 Fragments arranged by size: 1282 879 768 711 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 187 4134 187 Size: 3947 483 BsaAI yAC'GTr Cuts at: 1783 1783 Size: 4430 BsaBI GATnn'nnATC Cuts at: 6 316 1944 3981 6 Size: 310 1628 2037 455 Fragments arranged by size: 2037 1628 455 310 BsaHI Gr'CG_yC Cuts at: 1861 2109 2811 4307 1861 Size: 248 702 1496 1984 Fragments arranged by size: 1984 1496 702 248 BsaWI w'CCGG_w Cuts at: 1619 2140 2818 3092 3239 3650 1619 Size: 521 678 274 147 411 2399 Fragments arranged by size: 2399 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 984 1014 1131 1161 1307 1337 984 Size: 30 117 30 146 30 4077 Fragments arranged by size: 4077 146 117 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 142 142 Size: 4430 BseYI C'CCAG_C Cuts at: 1855 2613 3190 3686 1855 Size: 758 577 496 2599 Fragments arranged by size: 2599 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1001 1001 Size: 4430 BsiEI CG_ry'CG Cuts at: 104 1972 2018 3226 104 Size: 1868 46 1208 1308 Fragments arranged by size: 1868 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 524 1444 2222 2412 3204 524 Size: 920 778 190 792 1750 Fragments arranged by size: 1750 920 792 778 190 BsmI GAATG_Cn' Cuts at: 543 1400 543 Size: 857 3573 Bsp1286I G_dGCh'C Cuts at: 524 1042 1444 2055 2148 2222 2412 2474 3204 Size: 518 402 611 93 74 190 62 730 Cuts at: 3204 524 Size: 1750 Fragments arranged by size: 1750 730 611 518 402 190 93 74 62 BspEI T'CCGG_A Cuts at: 3650 3650 Size: 4430 BspHI T'CATG_A Cuts at: 459 669 3606 459 Size: 210 2937 1283 Fragments arranged by size: 2937 1283 210 BspMI ACCTGCnnnn'nnnn_ Cuts at: 164 1480 1694 1996 2377 164 Size: 1316 214 302 381 2217 Fragments arranged by size: 2217 1316 381 302 214 BsrBI CCG'CTC Cuts at: 50 2722 2776 4260 4425 50 Size: 2672 54 1484 165 55 Fragments arranged by size: 2672 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2342 2342 Size: 4430 BsrFI r'CCGG_y Cuts at: 860 2428 2609 2818 860 Size: 1568 181 209 2472 Fragments arranged by size: 2472 1568 209 181 BssSI C'ACGA_G Cuts at: 2701 3059 2701 Size: 358 4072 BstAPI GCAn_nnn'nTGC Cuts at: 1807 1807 Size: 4430 BstBI TT'CG_AA Cuts at: 2791 2791 Size: 4430 BstXI CCAn_nnnn'nTGG Cuts at: 177 177 Size: 4430 BstYI r'GATC_y Cuts at: 1 480 1455 1922 1945 2280 2526 3538 3624 Size: 479 975 467 23 335 246 1012 86 Cuts at: 3624 3636 3982 1 Size: 12 346 449 Fragments arranged by size: 1012 975 479 467 449 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 196 549 196 Size: 353 4077 BtgI C'CryG_G Cuts at: 1237 1237 Size: 4430 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2380 2561 2724 2380 Size: 181 163 4086 Fragments arranged by size: 4086 181 163 BtsI GCAGTG_nn' Cuts at: 1673 1673 Size: 4430 DraI TTT'AAA Cuts at: 1429 1429 Size: 4430 DrdI GACnn_nn'nnGTC Cuts at: 2136 2994 2136 Size: 858 3572 EaeI y'GGCC_r Cuts at: 354 2015 2189 2580 2607 3952 354 Size: 1661 174 391 27 1345 832 Fragments arranged by size: 1661 1345 832 391 174 27 EagI C'GGCC_G Cuts at: 2015 2015 Size: 4430 EarI CTCTTCn'nnn_ Cuts at: 1331 2453 2663 1331 Size: 1122 210 3098 Fragments arranged by size: 3098 1122 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2638 2948 3094 4240 2638 Size: 310 146 1146 2828 Fragments arranged by size: 2828 1146 310 146 EcoICRI GAG'CTC Cuts at: 1442 1442 Size: 4430 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 560 779 781 919 986 1133 1309 1439 2255 Size: 219 2 138 67 147 176 130 816 Cuts at: 2255 2687 3434 3702 560 Size: 432 747 268 1288 Fragments arranged by size: 1288 816 747 432 268 219 176 147 138 130 67 2 EcoNI CCTnn'n_nnAGG Cuts at: 578 806 578 Size: 228 4202 EcoO109I rG'GnC_Cy Cuts at: 1404 1569 1404 Size: 165 4265 EcoRI G'AATT_C Cuts at: 448 1095 1434 448 Size: 647 339 3444 Fragments arranged by size: 3444 647 339 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 299 2449 299 Size: 2150 2280 FspI TGC'GCA Cuts at: 2211 2211 Size: 4430 HaeII r_GCGC'y Cuts at: 918 1864 2112 3134 4113 918 Size: 946 248 1022 979 1235 Fragments arranged by size: 1235 1022 979 946 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 165 197 391 423 595 627 984 1016 2272 Size: 32 194 32 172 32 357 32 1256 Cuts at: 2272 2302 2304 2334 165 Size: 30 2 30 2261 Fragments arranged by size: 2261 1256 357 194 172 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1127 1281 1469 1127 Size: 154 188 4088 Fragments arranged by size: 4088 188 154 HindIII A'AGCT_T Cuts at: 664 664 Size: 4430 KasI G'GCGC_C Cuts at: 1860 2108 1860 Size: 248 4182 KpnI G_GTAC'C Cuts at: 574 1450 574 Size: 876 3554 MluI A'CGCG_T Cuts at: 3863 3863 Size: 4430 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3101 3285 3101 Size: 184 4246 MscI TGG'CCA Cuts at: 356 2191 356 Size: 1835 2595 MslI CAynn'nnrTG Cuts at: 2419 2546 2419 Size: 127 4303 MspA1I CmG'CkG Cuts at: 958 1541 1855 2215 3228 3473 958 Size: 583 314 360 1013 245 1915 Fragments arranged by size: 1915 1013 583 360 314 245 NaeI GCC'GGC Cuts at: 2611 2611 Size: 4430 NarI GG'CG_CC Cuts at: 1861 2109 1861 Size: 248 4182 NcoI C'CATG_G Cuts at: 1237 1237 Size: 4430 NgoMIV G'CCGG_C Cuts at: 2609 2609 Size: 4430 NspI r_CATG'y Cuts at: 1024 1279 1483 1983 2890 1024 Size: 255 204 500 907 2564 Fragments arranged by size: 2564 907 500 255 204 PciI A'CATG_T Cuts at: 1020 1275 2886 1020 Size: 255 1611 2564 Fragments arranged by size: 2564 1611 255 PmeI GTTT'AAAC Cuts at: 1429 1429 Size: 4430 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3569 3601 3569 Size: 32 4398 PshAI GACnn'nnGTC Cuts at: 1150 1150 Size: 4430 PstI C_TGCA'G Cuts at: 161 933 1477 2162 161 Size: 772 544 685 2429 Fragments arranged by size: 2429 772 685 544 PvuI CG_AT'CG Cuts at: 104 104 Size: 4430 PvuII CAG'CTG Cuts at: 958 1855 2215 958 Size: 897 360 3173 Fragments arranged by size: 3173 897 360 RsrII CG'GwC_CG Cuts at: 2625 2625 Size: 4430 SacI G_AGCT'C Cuts at: 1444 1444 Size: 4430 SalI G'TCGA_C Cuts at: 1467 1467 Size: 4430 SapI GCTCTTCn'nnn_ Cuts at: 1331 2453 2663 1331 Size: 1122 210 3098 Fragments arranged by size: 3098 1122 210 SbfI CC_TGCA'GG Cuts at: 1477 1477 Size: 4430 ScaI AGT'ACT Cuts at: 3947 3947 Size: 4430 SfcI C'TryA_G Cuts at: 33 157 929 1473 2158 3151 3342 33 Size: 124 772 544 685 993 191 1121 Fragments arranged by size: 1121 993 772 685 544 191 124 SfoI GGC'GCC Cuts at: 1862 2110 1862 Size: 248 4182 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4430 SmaI CCC'GGG Cuts at: 1452 1452 Size: 4430 SmlI C'TyrA_G Cuts at: 142 910 1585 2500 2992 3254 142 Size: 768 675 915 492 262 1318 Fragments arranged by size: 1318 915 768 675 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4430 SphI G_CATG'C Cuts at: 1483 1983 1483 Size: 500 3930 StyI C'CwwG_G Cuts at: 890 1237 1564 1918 890 Size: 347 327 354 3402 Fragments arranged by size: 3402 354 347 327 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2013 2146 2605 2814 2013 Size: 133 459 209 3629 Fragments arranged by size: 3629 459 209 133 TatI w'GTAC_w Cuts at: 146 3945 146 Size: 3799 631 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3947 3979 3947 Size: 32 4398 Tth111I GACn'n_nGTC Cuts at: 1029 2227 1029 Size: 1198 3232 XbaI T'CTAG_A Cuts at: 123 1461 123 Size: 1338 3092 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1925 1925 Size: 4430 XmaI C'CCGG_G Cuts at: 1450 1450 Size: 4430 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PshAI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AflII AhdI AleI AlfI AloI ApaI AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI