################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII BbeI BcgI BciVI BglI BsaHI BsiEI BsiWI BsmBI BsoBI BspLU11I BsrBI BssHII BssSI Bst1107I BstUI BstZ17I Bsu36I CpoI DraI DraIII EagI EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FseI FspI Hin1I HincII HindII HpaI KasI KpnI KspI MfeI MluI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PmeI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccIII 489 TCCGGA T^CCGGA AflII 967 CTTAAG C^TTAAG AflIII 1469 ACGTGT A^C[GA][CT]GT AgeI 932 ACCGGT A^CCGGT AhdI 2337 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 1997 GGGCCC GGGCC^C AspEI 2337 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 2076 TGGCCA TGG^CCA BamHI 529 GGATCC G^GATCC BbrPI 1468 CACGTG CAC^GTG BclI 1580 TGATCA T^GATCA BfrI 967 CTTAAG C^TTAAG BlpI 961 GCTCAGC GC^T[ACGT]AGC Bpu1102I 961 GCTCAGC GC^T[ACGT]AGC BsaAI 1468 CACGTG [CT]AC^GT[GA] BseAI 489 TCCGGA T^CCGGA Bsp1407I 2190 TGTACA T^GTACA BspDI 2893 ATCGAT AT^CGAT BspEI 489 TCCGGA T^CCGGA BspHI 1490 TCATGA T^CATGA BspMI 1620 GCAGGT ACCTGC(4/8) BsrDI 2768 GCAATG GCAATG(2/0) BsrGI 2190 TGTACA T^GTACA BstAPI 21 GCATTCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1148 TTCGAA TT^CGAA BstEII 415 GGTAACC G^GT[ACGT]ACC BstPI 415 GGTAACC G^GT[ACGT]ACC CelII 961 GCTCAGC GC^T[ACGT]AGC ClaI 2893 ATCGAT AT^CGAT DrdI 1390 GACCTGTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 2076 TGGCCA [CT]^GGCC[GA] Eam1105I 2337 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 415 GGTAACC G^GT[ACGT]ACC FbaI 1580 TGATCA T^GATCA HaeII 2857 AGCGCC [GA]GCGC^[CT] MluNI 2076 TGGCCA TGG^CCA MroI 489 TCCGGA T^CCGGA MscI 2076 TGGCCA TGG^CCA NspI 702 GCATGC [GA]CATG^[CT] NspV 1148 TTCGAA TT^CGAA PflMI 2810 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 932 ACCGGT A^CCGGT PmaCI 1468 CACGTG CAC^GTG PmlI 1468 CACGTG CAC^GTG PshAI 2143 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1490 TCATGA T^CATGA SapI 2635 GAAGAGC GCTCTTC(1/4) SexAI 419 ACCAGGT A^CC[AT]GGT SfuI 1148 TTCGAA TT^CGAA SmlI 967 CTTAAG C^T[CT][GA]AG SphI 702 GCATGC GCATG^C SspBI 2190 TGTACA T^GTACA Van91I 2810 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Alw44I 1473 GTGCAC G^TGCAC 2046 GTGCAC AlwNI 877 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1397 CAGTCGCTG ApaLI 1473 GTGCAC G^TGCAC 2046 GTGCAC AvrII 945 CCTAGG C^CTAGG 2592 CCTAGG BanI 1174 GGCACC G^G[CT][GA]CC 2155 GGTGCC BglII 2449 AGATCT A^GATCT 2708 AGATCT BlnI 945 CCTAGG C^CTAGG 2592 CCTAGG BsaBI 2074 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2554 GATGAGCATC BsaI 1125 GGTCTC GGTCTC(1/5) 2019 GGTCTC BsaWI 489 TCCGGA [AT]^CCGG[AT] 932 ACCGGT BsgI 1450 CTGCAC GTGCAG(16/14) 2051 CTGCAC BsmI 21 GCATTC GAATGC(1/-1) 1908 GCATTC BstXI 719 CCAAATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1818 CCAGCCTCTTGG CfoI 195 GCGC GCG^C 2858 GCGC EcoNI 562 CCTTCGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1165 CCTGTGTCAGG HgaI 541 GACGC GACGC(5/10) 2401 GACGC HhaI 195 GCGC GCG^C 2858 GCGC HinP1I 195 GCGC G^CGC 2858 GCGC MamI 2074 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2554 GATGAGCATC MslI 693 CATAGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2644 CACTTTGATG NcoI 2810 CCATGG C^CATGG 2933 CCATGG XcmI 1212 CCAGGACTGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2810 CCATGGAGTGGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr10I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII