(Circular) (Six-base) MAPSORT of: pF1KE2467.seq Check: 2388 from: 1 to: 3806 pF1KE2467 3806 bp With 182 enzymes: SgfI * March 29, 2013 15:36 .. AccI GT'mk_AC Cuts at: 389 844 389 Size: 455 3351 Acc65I G'GTAC_C Cuts at: 822 822 Size: 3806 AclI AA'CG_TT Cuts at: 3646 3646 Size: 3806 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1631 2063 2810 3078 1631 Size: 432 747 268 2359 Fragments arranged by size: 2359 747 432 268 AflIII A'CryG_T Cuts at: 1158 2262 3239 1158 Size: 1104 977 1725 Fragments arranged by size: 1725 1104 977 AgeI A'CCGG_T Cuts at: 2194 2194 Size: 3806 AlwNI CAG_nnn'CTG Cuts at: 1111 2678 1111 Size: 1567 2239 ApaLI G'TGCA_C Cuts at: 2576 2576 Size: 3806 ApoI r'AATT_y Cuts at: 129 628 810 3159 3415 129 Size: 499 182 2349 256 520 Fragments arranged by size: 2349 520 499 256 182 AseI AT'TA_AT Cuts at: 20 20 Size: 3806 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3806 AvaI C'yCGr_G Cuts at: 826 826 Size: 3806 BamHI G'GATC_C Cuts at: 831 831 Size: 3806 BanI G'GyrC_C Cuts at: 822 1236 1484 1519 822 Size: 414 248 35 3109 Fragments arranged by size: 3109 414 248 35 BanII G_rGCy'C Cuts at: 709 820 1850 709 Size: 111 1030 2665 Fragments arranged by size: 2665 1030 111 BbeI G_GCGC'C Cuts at: 1240 1488 1240 Size: 248 3558 BciVI GTATCCnnnnn_n' Cuts at: 1704 2465 3806 1704 Size: 761 1341 1704 Fragments arranged by size: 1704 1341 761 BclI T'GATC_A Cuts at: 1326 1326 Size: 3806 BglII A'GATC_T Cuts at: 1321 3358 1321 Size: 2037 1769 BlpI GC'TnA_GC Cuts at: 918 918 Size: 3806 Bme1580I G_kGCm'C Cuts at: 780 1431 1524 2580 780 Size: 651 93 1056 2006 Fragments arranged by size: 2006 1056 651 93 BmrI ACTGGGnnnn_n' Cuts at: 190 1018 1099 1434 3578 190 Size: 828 81 335 2144 418 Fragments arranged by size: 2144 828 418 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2173 2173 Size: 3806 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 218 982 1861 2353 2651 218 Size: 764 879 492 298 1373 Fragments arranged by size: 1373 879 764 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 429 3510 429 Size: 3081 725 BsaAI yAC'GTr Cuts at: 387 1159 387 Size: 772 3034 BsaBI GATnn'nnATC Cuts at: 6 1320 3357 6 Size: 1314 2037 455 Fragments arranged by size: 2037 1314 455 BsaHI Gr'CG_yC Cuts at: 1237 1485 2187 3683 1237 Size: 248 702 1496 1360 Fragments arranged by size: 1496 1360 702 248 BsaWI w'CCGG_w Cuts at: 995 1516 2194 2468 2615 3026 995 Size: 521 678 274 147 411 1775 Fragments arranged by size: 1775 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 1231 1989 2566 3062 1231 Size: 758 577 496 1975 Fragments arranged by size: 1975 758 577 496 BsiEI CG_ry'CG Cuts at: 104 784 1348 1394 2602 104 Size: 680 564 46 1208 1308 Fragments arranged by size: 1308 1208 680 564 46 BsiHKAI G_wGCw'C Cuts at: 820 1598 1788 2580 820 Size: 778 190 792 2046 Fragments arranged by size: 2046 792 778 190 Bsp1286I G_dGCh'C Cuts at: 709 780 820 1431 1524 1598 1788 1850 2580 Size: 71 40 611 93 74 190 62 730 Cuts at: 2580 709 Size: 1935 Fragments arranged by size: 1935 730 611 190 93 74 71 62 40 BspEI T'CCGG_A Cuts at: 3026 3026 Size: 3806 BspHI T'CATG_A Cuts at: 2982 2982 Size: 3806 BspMI ACCTGCnnnn'nnnn_ Cuts at: 856 1070 1372 1753 856 Size: 214 302 381 2909 Fragments arranged by size: 2909 381 302 214 BsrBI CCG'CTC Cuts at: 50 2098 2152 3636 3801 50 Size: 2048 54 1484 165 55 Fragments arranged by size: 2048 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 192 1718 192 Size: 1526 2280 BsrFI r'CCGG_y Cuts at: 783 1804 1985 2194 783 Size: 1021 181 209 2395 Fragments arranged by size: 2395 1021 209 181 BssSI C'ACGA_G Cuts at: 2077 2435 2077 Size: 358 3448 BstAPI GCAn_nnn'nTGC Cuts at: 1183 1183 Size: 3806 BstBI TT'CG_AA Cuts at: 2167 2167 Size: 3806 BstYI r'GATC_y Cuts at: 1 519 831 1298 1321 1656 1902 2914 3000 Size: 518 312 467 23 335 246 1012 86 Cuts at: 3000 3012 3358 1 Size: 12 346 449 Fragments arranged by size: 1012 518 467 449 346 335 312 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 772 772 Size: 3806 BtgI C'CryG_G Cuts at: 107 107 Size: 3806 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1756 1937 2100 1756 Size: 181 163 3462 Fragments arranged by size: 3462 181 163 BtsI GCAGTG_nn' Cuts at: 254 1049 254 Size: 795 3011 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 660 695 660 Size: 35 3771 DraI TTT'AAA Cuts at: 805 805 Size: 3806 DrdI GACnn_nn'nnGTC Cuts at: 1512 2370 1512 Size: 858 2948 EaeI y'GGCC_r Cuts at: 531 781 1391 1565 1956 1983 3328 531 Size: 250 610 174 391 27 1345 1009 Fragments arranged by size: 1345 1009 610 391 250 174 27 EagI C'GGCC_G Cuts at: 781 1391 781 Size: 610 3196 EarI CTCTTCn'nnn_ Cuts at: 199 427 1829 2039 199 Size: 228 1402 210 1966 Fragments arranged by size: 1966 1402 228 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2014 2324 2470 3616 2014 Size: 310 146 1146 2204 Fragments arranged by size: 2204 1146 310 146 EcoICRI GAG'CTC Cuts at: 818 818 Size: 3806 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1631 2063 2810 3078 1631 Size: 432 747 268 2359 Fragments arranged by size: 2359 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 945 945 Size: 3806 EcoRI G'AATT_C Cuts at: 810 810 Size: 3806 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1825 1825 Size: 3806 FspI TGC'GCA Cuts at: 1587 1587 Size: 3806 HaeII r_GCGC'y Cuts at: 1240 1488 2510 3489 1240 Size: 248 1022 979 1557 Fragments arranged by size: 1557 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1648 1678 1680 1710 1648 Size: 30 2 30 3744 Fragments arranged by size: 3744 30 30 2 HincII GTy'rAC Cuts at: 465 669 845 465 Size: 204 176 3426 Fragments arranged by size: 3426 204 176 KasI G'GCGC_C Cuts at: 1236 1484 1236 Size: 248 3558 KpnI G_GTAC'C Cuts at: 826 826 Size: 3806 MluI A'CGCG_T Cuts at: 3239 3239 Size: 3806 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2477 2661 2477 Size: 184 3622 MscI TGG'CCA Cuts at: 533 1567 533 Size: 1034 2772 MslI CAynn'nnrTG Cuts at: 760 1795 1922 760 Size: 1035 127 2644 Fragments arranged by size: 2644 1035 127 MspA1I CmG'CkG Cuts at: 917 1231 1591 2604 2849 917 Size: 314 360 1013 245 1874 Fragments arranged by size: 1874 1013 360 314 245 NaeI GCC'GGC Cuts at: 785 1987 785 Size: 1202 2604 NarI GG'CG_CC Cuts at: 1237 1485 1237 Size: 248 3558 NcoI C'CATG_G Cuts at: 107 107 Size: 3806 NgoMIV G'CCGG_C Cuts at: 783 1985 783 Size: 1202 2604 NspI r_CATG'y Cuts at: 692 859 1359 2266 692 Size: 167 500 907 2232 Fragments arranged by size: 2232 907 500 167 PciI A'CATG_T Cuts at: 2262 2262 Size: 3806 PmeI GTTT'AAAC Cuts at: 805 805 Size: 3806 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2945 2977 2945 Size: 32 3774 PstI C_TGCA'G Cuts at: 853 1538 853 Size: 685 3121 PvuI CG_AT'CG Cuts at: 104 104 Size: 3806 PvuII CAG'CTG Cuts at: 1231 1591 1231 Size: 360 3446 RsrII CG'GwC_CG Cuts at: 2001 2001 Size: 3806 SacI G_AGCT'C Cuts at: 820 820 Size: 3806 SalI G'TCGA_C Cuts at: 843 843 Size: 3806 SapI GCTCTTCn'nnn_ Cuts at: 1829 2039 1829 Size: 210 3596 SbfI CC_TGCA'GG Cuts at: 853 853 Size: 3806 ScaI AGT'ACT Cuts at: 3323 3323 Size: 3806 SfcI C'TryA_G Cuts at: 33 410 849 1534 2527 2718 33 Size: 377 439 685 993 191 1121 Fragments arranged by size: 1121 993 685 439 377 191 SfoI GGC'GCC Cuts at: 1238 1486 1238 Size: 248 3558 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3806 SmaI CCC'GGG Cuts at: 828 828 Size: 3806 SmlI C'TyrA_G Cuts at: 233 961 1876 2368 2630 233 Size: 728 915 492 262 1409 Fragments arranged by size: 1409 915 728 492 262 SnaBI TAC'GTA Cuts at: 387 387 Size: 3806 SpeI A'CTAG_T Cuts at: 67 67 Size: 3806 SphI G_CATG'C Cuts at: 859 1359 859 Size: 500 3306 StyI C'CwwG_G Cuts at: 107 940 1294 107 Size: 833 354 2619 Fragments arranged by size: 2619 833 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 229 1389 1522 1981 2190 229 Size: 1160 133 459 209 1845 Fragments arranged by size: 1845 1160 459 209 133 TatI w'GTAC_w Cuts at: 3321 3321 Size: 3806 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3323 3355 3323 Size: 32 3774 Tth111I GACn'n_nGTC Cuts at: 1603 1603 Size: 3806 XbaI T'CTAG_A Cuts at: 837 837 Size: 3806 XcmI CCAnnnn_n'nnnnTGG Cuts at: 585 1301 585 Size: 716 3090 XmaI C'CCGG_G Cuts at: 826 826 Size: 3806 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI XhoI XmnI ZraI