Restriction map for pF1KE9652
##################################
# NO CUT ENZYMES (   52 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        AscI        
Asp718I     AvrII       BbeI        BbrPI       BbvCI       
BcgI        BlnI        BsiEI       BsiWI       BspDI       
BspLU11I    BssHII      BstBI       ClaI        CpoI        
EagI        EclXI       Eco52I      EcoT22I     FseI        
KasI        KpnI        MluI        NarI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        RsrII       SalI        SfiI        
SfoI        SfuI        SgrAI       SpeI        SphI        
StuI        SwaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccIII       4655  TCCGGA               T^CCGGA
AflIII       7334  ACACGT               A^C[GA][CT]GT
AhdI         4440  GACCATTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       7436  GTGCAC               G^TGCAC
Aor51HI      2096  AGCGCT               AGC^GCT
ApaLI        7436  GTGCAC               G^TGCAC
AseI         3646  ATTAAT               AT^TAAT
AsnI         3646  ATTAAT               AT^TAAT
AspEI        4440  GACCATTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        3272  TGCGCA               TGC^GCA
BclI         2768  TGATCA               T^GATCA
BlpI          559  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      559  GCTCAGC              GC^T[ACGT]AGC
BsaAI        2167  TACGTA               [CT]AC^GT[GA]
BsaWI        4655  TCCGGA               [AT]^CCGG[AT]
BseAI        4655  TCCGGA               T^CCGGA
BsmBI        1719  GAGACG               CGTCTC(1/5)
Bsp1407I     1131  TGTACA               T^GTACA
BspEI        4655  TCCGGA               T^CCGGA
BspHI        3360  TCATGA               T^CATGA
BsrGI        1131  TGTACA               T^GTACA
Bst1107I     5964  GTATAC               GTA^TAC
BstEII       5187  GGTGACC              G^GT[ACGT]ACC
BstPI        5187  GGTGACC              G^GT[ACGT]ACC
BstZ17I      5964  GTATAC               GTA^TAC
CelII         559  GCTCAGC              GC^T[ACGT]AGC
Eam1105I     4440  GACCATTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2096  AGCGCT               AGC^GCT
EcoO65I      5187  GGTGACC              G^GT[ACGT]ACC
EcoRV        4633  GATATC               GAT^ATC
FbaI         2768  TGATCA               T^GATCA
FspI         3272  TGCGCA               TGC^GCA
HpaI         6786  GTTAAC               GTT^AAC
KspI         1422  CCGCGG               CCGC^GG
MfeI         5000  CAATTG               C^AATTG
MroI         4655  TCCGGA               T^CCGGA
MunI         5000  CAATTG               C^AATTG
NaeI         3394  GCCGGC               GCC^GGC
NgoMIV       3394  GCCGGC               G^CCGGC
PshBI        3646  ATTAAT               AT^TAAT
RcaI         3360  TCATGA               T^CATGA
SacI         1966  GAGCTC               GAGCT^C
SacII        1422  CCGCGG               CCGC^GG
ScaI         5534  AGTACT               AGT^ACT
SmaI         3626  CCCGGG               CCC^GGG
SnaBI        2167  TACGTA               TAC^GTA
SspBI        1131  TGTACA               T^GTACA
SspI         4495  AATATT               AAT^ATT
XbaI         7165  TCTAGA               T^CTAGA
XmaCI        3626  CCCGGG               C^CCGGG
XmaI         3626  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccII        1423  CGCG                 CG^CG
             4214  CGCG                
AflII         484  CTTAAG               C^TTAAG
             3073  CTTAAG              
AocI         4758  CCTGAGG              CC^T[ACGT]AGG
             7606  CCTGAGG             
ApaI          796  GGGCCC               GGGCC^C
             7253  GGGCCC              
AspI         1119  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6493  GACCTCGTC           
BamHI        4652  GGATCC               G^GATCC
             6941  GGATCC              
BciVI        2078  GGATAC               GTATCC(6/5)
             6133  GTATCC              
BfrI          484  CTTAAG               C^TTAAG
             3073  CTTAAG              
BsrBI        6330  CCGCTC               CCGCTC(-3/-3)
             4282  GAGCGG              
BstUI        1423  CGCG                 CG^CG
             4214  CGCG                
Bsu36I       4758  CCTGAGG              CC^T[ACGT]AGG
             7606  CCTGAGG             
DraIII       2911  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             6082  CACGAGGTG           
Eco81I       4758  CCTGAGG              CC^T[ACGT]AGG
             7606  CCTGAGG             
HaeII        2096  AGCGCT               [GA]GCGC^[CT]
             5681  GGCGCT              
HincII       5053  GTTGAC               GT[CT]^[GA]AC
             6786  GTTAAC              
HindII       5053  GTTGAC               GT[CT]^[GA]AC
             6786  GTTAAC              
MvnI         1423  CGCG                 CG^CG
             4214  CGCG                
NdeI         3932  CATATG               CA^TATG
             4827  CATATG              
NspI          909  GCATGT               [GA]CATG^[CT]
             3239  ACATGC              
PaeR7I       1442  CTCGAG               C^TCGAG
             1688  CTCGAG              
PflFI        1119  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6493  GACCTCGTC           
SbfI         3372  CCTGCAGG             CCTGCA^GG
             3708  CCTGCAGG            
Sse8387I     3372  CCTGCAGG             CCTGCA^GG
             3708  CCTGCAGG            
Tth111I      1119  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6493  GACCTCGTC           
XcmI         3812  CCAGCCTCAAGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4861  CCAACAACTTGGTGG     
XhoI         1442  CTCGAG               C^TCGAG
             1688  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  129 enzymes) #
#########################################

AccI        AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApoI        Asp700I     
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BglI        BglII       BmyI        BpmI        BpuAI       
BsaBI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspMI       
BsrDI       BsrFI       BsrI        BssKI       BssSI       
BstAPI      BstF5I      BstNI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraI        DraII       DrdI        DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRI       
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MamI        MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SexAI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoII       XmnI        
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