Restriction map for
pF1KE9399
(Linear) (Six-base) MAPSORT of: KIEE9399.seq Check: 3743 from: 1 to: 8145
KIEE9399.orf
With 182 enzymes: SgfI *
August 18, 2017 10:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4174 8145
Size: 4174 3971
AatII G_ACGT'C
Cuts at: 0 3947 7717 8145
Size: 3947 3770 428
Fragments arranged by size:
3947 3770 428
AccI GT'mk_AC
Cuts at: 0 3002 7736 8145
Size: 3002 4734 409
Fragments arranged by size:
4734 3002 409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1582 1996 2042 2552 2681 4513 4954 5930
Size: 1582 414 46 510 129 1832 441 976
Cuts at: 5930 8145
Size: 2215
Fragments arranged by size:
2215 1832 1582 976 510 441 414 129
46
AfeI AGC'GCT
Cuts at: 0 3808 4363 6150 8125 8145
Size: 3808 555 1787 1975 20
Fragments arranged by size:
3808 1975 1787 555 20
AflII C'TTAA_G
Cuts at: 0 7705 8145
Size: 7705 440
AflIII A'CryG_T
Cuts at: 0 2607 2919 3548 4821 7565 8145
Size: 2607 312 629 1273 2744 580
Fragments arranged by size:
2744 2607 1273 629 580 312
AhdI GACnn_n'nnGTC
Cuts at: 0 2934 8145
Size: 2934 5211
AleI CACnn'nnGTG
Cuts at: 0 3583 3761 7961 8145
Size: 3583 178 4200 184
Fragments arranged by size:
4200 3583 184 178
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 705 2896 4078 4441 7952 8116 8145
Size: 705 2191 1182 363 3511 164 29
Fragments arranged by size:
3511 2191 1182 705 363 164 29
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2359 2391 4855 4887 8145
Size: 2359 32 2464 32 3258
Fragments arranged by size:
3258 2464 2359 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 576 826 1080 2211 2220 2244 2794 2821
Size: 576 250 254 1131 9 24 550 27
Cuts at: 2821 3352 3971 4257 5012 5520 8145
Size: 531 619 286 755 508 2625
Fragments arranged by size:
2625 1131 755 619 576 550 531 508
286 254 250 27 24 9
ApaI G_GGCC'C
Cuts at: 0 307 600 815 1947 2977 3161 3191 3298
Size: 307 293 215 1132 1030 184 30 107
Cuts at: 3298 3598 3845 4224 4282 5923 6058 6309 6507
Size: 300 247 379 58 1641 135 251 198
Cuts at: 6507 7052 8145
Size: 545 1093
Fragments arranged by size:
1641 1132 1093 1030 545 379 307 300
293 251 247 215 198 184 135 107
58 30
ApaLI G'TGCA_C
Cuts at: 0 3502 3981 4390 4742 5413 7554 8145
Size: 3502 479 409 352 671 2141 591
Fragments arranged by size:
3502 2141 671 591 479 409 352
ApoI r'AATT_y
Cuts at: 0 3748 5336 8145
Size: 3748 1588 2809
Fragments arranged by size:
3748 2809 1588
AvaI C'yCGr_G
Cuts at: 0 31 60 181 306 446 656 1454 1844
Size: 31 29 121 125 140 210 798 390
Cuts at: 1844 2078 2575 2645 3161 3190 3289 3323 3857
Size: 234 497 70 516 29 99 34 534
Cuts at: 3857 4901 5000 6057 6233 7334 7529 7982 8145
Size: 1044 99 1057 176 1101 195 453 163
Fragments arranged by size:
1101 1057 1044 798 534 516 497 453
390 234 210 195 176 163 140 125
121 99 99 70 34 31 29 29
AvrII C'CTAG_G
Cuts at: 0 6360 8145
Size: 6360 1785
BamHI G'GATC_C
Cuts at: 0 3781 4674 4701 8145
Size: 3781 893 27 3444
Fragments arranged by size:
3781 3444 893 27
BanI G'GyrC_C
Cuts at: 0 76 603 1471 2655 3416 3875 4942 5330
Size: 76 527 868 1184 761 459 1067 388
Cuts at: 5330 5820 6414 7145 8116 8145
Size: 490 594 731 971 29
Fragments arranged by size:
1184 1067 971 868 761 731 594 527
490 459 388 76 29
BanII G_rGCy'C
Cuts at: 0 182 218 307 600 638 755 815 902
Size: 182 36 89 293 38 117 60 87
Cuts at: 902 1054 1947 2019 2172 2464 2813 2977 3161
Size: 152 893 72 153 292 349 164 184
Cuts at: 3161 3191 3290 3298 3598 3845 4112 4224 4267
Size: 30 99 8 300 247 267 112 43
Cuts at: 4267 4282 4487 4928 5441 5493 5589 5923 6005
Size: 15 205 441 513 52 96 334 82
Cuts at: 6005 6058 6165 6309 6507 6548 7028 7052 7174
Size: 53 107 144 198 41 480 24 122
Cuts at: 7174 7446 7970 8057 8145
Size: 272 524 87 88
Fragments arranged by size:
893 524 513 480 441 349 334 300
293 292 272 267 247 205 198 184
182 164 153 152 144 122 117 112
107 99 96 89 88 87 87 82
72 60 53 52 43 41 38 36
30 24 15 8
BbeI G_GCGC'C
Cuts at: 0 80 3420 3879 4946 6418 8120 8145
Size: 80 3340 459 1067 1472 1702 25
Fragments arranged by size:
3340 1702 1472 1067 459 80 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 1529 1652 3079 4043 4127 4845 5031 5918
Size: 1529 123 1427 964 84 718 186 887
Cuts at: 5918 7717 8145
Size: 1799 428
Fragments arranged by size:
1799 1529 1427 964 887 718 428 186
123 84
BbvCI CC'TCA_GC
Cuts at: 0 1160 2104 4680 4975 5810 6215 8145
Size: 1160 944 2576 295 835 405 1930
Fragments arranged by size:
2576 1930 1160 944 835 405 295
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 6214 6248 8145
Size: 6214 34 1897
Fragments arranged by size:
6214 1897 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2715 6918 8145
Size: 2715 4203 1227
Fragments arranged by size:
4203 2715 1227
BclI T'GATC_A
Cuts at: 0 911 8145
Size: 911 7234
BfrBI ATG'CAT
Cuts at: 0 3820 7929 8145
Size: 3820 4109 216
Fragments arranged by size:
4109 3820 216
BglI GCCn_nnn'nGGC
Cuts at: 0 863 872 4200 5358 6031 6053 8145
Size: 863 9 3328 1158 673 22 2092
Fragments arranged by size:
3328 2092 1158 863 673 22 9
BglII A'GATC_T
Cuts at: 0 1979 2504 8145
Size: 1979 525 5641
Fragments arranged by size:
5641 1979 525
BlpI GC'TnA_GC
Cuts at: 0 2066 2204 2458 4261 6790 8145
Size: 2066 138 254 1803 2529 1355
Fragments arranged by size:
2529 2066 1803 1355 254 138
Bme1580I G_kGCm'C
Cuts at: 0 307 600 815 1474 1602 1758 1947 2685
Size: 307 293 215 659 128 156 189 738
Cuts at: 2685 2977 3161 3191 3298 3506 3598 3845 3985
Size: 292 184 30 107 208 92 247 140
Cuts at: 3985 4224 4282 4394 4746 5001 5417 5603 5825
Size: 239 58 112 352 255 416 186 222
Cuts at: 5825 5923 6058 6247 6309 6507 7052 7558 8145
Size: 98 135 189 62 198 545 506 587
Fragments arranged by size:
738 659 587 545 506 416 352 307
293 292 255 247 239 222 215 208
198 189 189 186 184 156 140 135
128 112 107 98 92 62 58 30
BmgBI CAC'GTC
Cuts at: 0 3759 4824 7660 8145
Size: 3759 1065 2836 485
Fragments arranged by size:
3759 2836 1065 485
BmrI ACTGGGnnnn_n'
Cuts at: 0 395 1297 1771 3948 4095 4293 6634 7134
Size: 395 902 474 2177 147 198 2341 500
Cuts at: 7134 8145
Size: 1011
Fragments arranged by size:
2341 2177 1011 902 500 474 395 198
147
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1491 2583 3219 4128 5520 8145
Size: 1491 1092 636 909 1392 2625
Fragments arranged by size:
2625 1491 1392 1092 909 636
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1208 1380 2045 2156 2486 2843 3110 3709
Size: 1208 172 665 111 330 357 267 599
Cuts at: 3709 3817 3911 3977 4210 4234 4487 4652 4744
Size: 108 94 66 233 24 253 165 92
Cuts at: 4744 5313 5362 5404 5507 5546 6179 7039 7693
Size: 569 49 42 103 39 633 860 654
Cuts at: 7693 8145
Size: 452
Fragments arranged by size:
1208 860 665 654 633 599 569 452
357 330 267 253 233 172 165 111
108 103 94 92 66 49 42 39
24
Bpu10I CC'TnA_GC
Cuts at: 0 749 1160 2030 2072 2104 4213 4680 4975
Size: 749 411 870 42 32 2109 467 295
Cuts at: 4975 5495 5810 5815 6215 6542 7645 8145
Size: 520 315 5 400 327 1103 500
Fragments arranged by size:
2109 1103 870 749 520 500 467 411
400 327 315 295 42 32 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2371 3388 4861 7153 8145
Size: 2371 1017 1473 2292 992
Fragments arranged by size:
2371 2292 1473 1017 992
BsaI GGTCTCn'nnnn_
Cuts at: 0 659 1680 4441 4639 5699 8145
Size: 659 1021 2761 198 1060 2446
Fragments arranged by size:
2761 2446 1060 1021 659 198
BsaAI yAC'GTr
Cuts at: 0 1391 1753 2920 7995 8145
Size: 1391 362 1167 5075 150
Fragments arranged by size:
5075 1391 1167 362 150
BsaBI GATnn'nnATC
Cuts at: 0 7801 8145
Size: 7801 344
BsaHI Gr'CG_yC
Cuts at: 0 77 2734 3312 3417 3876 3944 4526 4943
Size: 77 2657 578 105 459 68 582 417
Cuts at: 4943 6415 7714 7919 8117 8145
Size: 1472 1299 205 198 28
Fragments arranged by size:
2657 1472 1299 582 578 459 417 205
198 105 77 68 28
BsaWI w'CCGG_w
Cuts at: 0 1518 2525 3005 7083 7547 8145
Size: 1518 1007 480 4078 464 598
Fragments arranged by size:
4078 1518 1007 598 480 464
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1254 1284 1464 1494 1989 2019 2359 2389
Size: 1254 30 180 30 495 30 340 30
Cuts at: 2389 4855 4885 5263 5293 5932 5962 7285 7315
Size: 2466 30 378 30 639 30 1323 30
Cuts at: 7315 8145
Size: 830
Fragments arranged by size:
2466 1323 1254 830 639 495 378 340
180 30 30 30 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 346 373 376 476 479 1177 1211
Size: 343 3 27 3 100 3 698 34
Cuts at: 1211 1229 1259 1361 1366 1469 1596 1754 1850
Size: 18 30 102 5 103 127 158 96
Cuts at: 1850 1908 1991 1994 1997 2033 2374 2380 2494
Size: 58 83 3 3 36 341 6 114
Cuts at: 2494 3181 3208 3296 3370 3706 4453 4873 5410
Size: 687 27 88 74 336 747 420 537
Cuts at: 5410 5549 5783 6088 6091 6412 6706 7513 8065
Size: 139 234 305 3 321 294 807 552
Cuts at: 8065 8145
Size: 80
Fragments arranged by size:
807 747 698 687 552 537 420 343
341 336 321 305 294 234 158 139
127 114 103 102 100 96 88 83
80 74 58 36 34 30 27 27
18 6 5 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 0 210 299 428 1383 1426 1495 1866 2237
Size: 210 89 129 955 43 69 371 371
Cuts at: 2237 3151 3713 3844 3986 4180 5718 5746 5991
Size: 914 562 131 142 194 1538 28 245
Cuts at: 5991 6028 6145 6465 6588 6613 7030 7445 7586
Size: 37 117 320 123 25 417 415 141
Cuts at: 7586 8145
Size: 559
Fragments arranged by size:
1538 955 914 562 559 417 415 371
371 320 245 210 194 142 141 131
129 123 117 89 69 43 37 28
25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 2422 2944 4278 4309 6244 7660 8145
Size: 589 1833 522 1334 31 1935 1416 485
Fragments arranged by size:
1935 1833 1416 1334 589 522 485 31
BsiEI CG_ry'CG
Cuts at: 0 70 888 6858 8145
Size: 70 818 5970 1287
Fragments arranged by size:
5970 1287 818 70
BsiHKAI G_wGCw'C
Cuts at: 0 1396 3506 3578 3985 4250 4267 4394 4746
Size: 1396 2110 72 407 265 17 127 352
Cuts at: 4746 4928 5192 5281 5299 5417 5493 7174 7558
Size: 182 264 89 18 118 76 1681 384
Cuts at: 7558 7601 8057 8145
Size: 43 456 88
Fragments arranged by size:
2110 1681 1396 456 407 384 352 265
264 182 127 118 89 88 76 72
43 18 17
BsmI GAATG_Cn'
Cuts at: 0 4687 8145
Size: 4687 3458
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1791 8145
Size: 1791 6354
Bsp1286I G_dGCh'C
Cuts at: 0 182 218 307 600 638 755 815 902
Size: 182 36 89 293 38 117 60 87
Cuts at: 902 1054 1396 1474 1602 1758 1947 2019 2172
Size: 152 342 78 128 156 189 72 153
Cuts at: 2172 2464 2685 2813 2977 3161 3191 3290 3298
Size: 292 221 128 164 184 30 99 8
Cuts at: 3298 3506 3578 3598 3845 3985 4112 4224 4250
Size: 208 72 20 247 140 127 112 26
Cuts at: 4250 4267 4282 4394 4487 4746 4928 5001 5192
Size: 17 15 112 93 259 182 73 191
Cuts at: 5192 5281 5299 5417 5441 5493 5589 5603 5825
Size: 89 18 118 24 52 96 14 222
Cuts at: 5825 5923 6005 6058 6165 6247 6309 6507 6548
Size: 98 82 53 107 82 62 198 41
Cuts at: 6548 7028 7052 7174 7446 7558 7601 7970 8057
Size: 480 24 122 272 112 43 369 87
Cuts at: 8057 8145
Size: 88
Fragments arranged by size:
480 369 342 293 292 272 259 247
222 221 208 198 191 189 184 182
182 164 156 153 152 140 128 128
127 122 118 117 112 112 112 107
99 98 96 93 89 89 88 87
87 82 82 78 73 72 72 62
60 53 52 43 41 38 36 30
26 24 24 20 18 17 15 14
8
BspEI T'CCGG_A
Cuts at: 0 1518 7083 7547 8145
Size: 1518 5565 464 598
Fragments arranged by size:
5565 1518 598 464
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 4174 6332 7133 8145
Size: 4174 2158 801 1012
Fragments arranged by size:
4174 2158 1012 801
BsrBI CCG'CTC
Cuts at: 0 1443 1604 2562 3702 4217 7829 8145
Size: 1443 161 958 1140 515 3612 316
Fragments arranged by size:
3612 1443 1140 958 515 316 161
BsrDI GCAATG_nn'
Cuts at: 0 7941 8145
Size: 7941 204
BsrFI r'CCGG_y
Cuts at: 0 65 79 233 253 1409 3365 6857 8145
Size: 65 14 154 20 1156 1956 3492 1288
Fragments arranged by size:
3492 1956 1288 1156 154 65 20 14
BsrGI T'GTAC_A
Cuts at: 0 4021 4160 4796 8145
Size: 4021 139 636 3349
Fragments arranged by size:
4021 3349 636 139
BssSI C'ACGA_G
Cuts at: 0 3637 8145
Size: 3637 4508
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2220 2229 3752 7596 7880 8145
Size: 2220 9 1523 3844 284 265
Fragments arranged by size:
3844 2220 1523 284 265 9
BstEII G'GTnAC_C
Cuts at: 0 2130 8145
Size: 2130 6015
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3763 4275 5754 5780 5991 7671 8145
Size: 3763 512 1479 26 211 1680 474
Fragments arranged by size:
3763 1680 1479 512 474 211 26
BstYI r'GATC_y
Cuts at: 0 1033 1979 2504 3781 3884 4130 4674 4701
Size: 1033 946 525 1277 103 246 544 27
Cuts at: 4701 8145
Size: 3444
Fragments arranged by size:
3444 1277 1033 946 544 525 246 103
27
BstZ17I GTA'TAC
Cuts at: 0 3003 8145
Size: 3003 5142
Bsu36I CC'TnA_GG
Cuts at: 0 1810 2568 3107 3353 5862 6317 6932 7604
Size: 1810 758 539 246 2509 455 615 672
Cuts at: 7604 8145
Size: 541
Fragments arranged by size:
2509 1810 758 672 615 541 539 455
246
BtgI C'CryG_G
Cuts at: 0 70 96 259 858 867 882 1505 1535
Size: 70 26 163 599 9 15 623 30
Cuts at: 1535 2651 4946 5353 6035 6048 6677 6731 7518
Size: 1116 2295 407 682 13 629 54 787
Cuts at: 7518 7682 7752 8145
Size: 164 70 393
Fragments arranged by size:
2295 1116 787 682 629 623 599 407
393 164 163 70 70 54 30 26
15 13 9
BtsI GCAGTG_nn'
Cuts at: 0 345 2090 2854 2998 3340 4072 4282 4630
Size: 345 1745 764 144 342 732 210 348
Cuts at: 4630 5530 6801 6957 7263 8145
Size: 900 1271 156 306 882
Fragments arranged by size:
1745 1271 900 882 764 732 348 345
342 306 210 156 144
ClaI AT'CG_AT
Cuts at: 0 2417 8145
Size: 2417 5728
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3759 3794 8145
Size: 3759 35 4351
Fragments arranged by size:
4351 3759 35
DraIII CAC_nnn'GTG
Cuts at: 0 1753 3731 4089 4301 7596 8145
Size: 1753 1978 358 212 3295 549
Fragments arranged by size:
3295 1978 1753 549 358 212
DrdI GACnn_nn'nnGTC
Cuts at: 0 3773 5035 5738 6123 8145
Size: 3773 1262 703 385 2022
Fragments arranged by size:
3773 2022 1262 703 385
EaeI y'GGCC_r
Cuts at: 0 67 676 885 2486 4374 5350 5536 5981
Size: 67 609 209 1601 1888 976 186 445
Cuts at: 5981 6855 7424 7685 8145
Size: 874 569 261 460
Fragments arranged by size:
1888 1601 976 874 609 569 460 445
261 209 186 67
EagI C'GGCC_G
Cuts at: 0 67 885 6855 8145
Size: 67 818 5970 1290
Fragments arranged by size:
5970 1290 818 67
EarI CTCTTCn'nnn_
Cuts at: 0 1020 1091 1130 1184 1396 1789 5263 5363
Size: 1020 71 39 54 212 393 3474 100
Cuts at: 5363 6008 7175 7622 8145
Size: 645 1167 447 523
Fragments arranged by size:
3474 1167 1020 645 523 447 393 212
100 71 54 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 116 185 208 229 1453 4861 5098 6117
Size: 116 69 23 21 1224 3408 237 1019
Cuts at: 6117 6736 8145
Size: 619 1409
Fragments arranged by size:
3408 1409 1224 1019 619 237 116 69
23 21
EcoICRI GAG'CTC
Cuts at: 0 4265 4926 5491 7172 8055 8145
Size: 4265 661 565 1681 883 90
Fragments arranged by size:
4265 1681 883 661 565 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1208 1380 1582 1996 2042 2045 2156 2486
Size: 1208 172 202 414 46 3 111 330
Cuts at: 2486 2552 2681 2843 3110 3709 3817 3911 3977
Size: 66 129 162 267 599 108 94 66
Cuts at: 3977 4210 4234 4487 4513 4652 4744 4954 5313
Size: 233 24 253 26 139 92 210 359
Cuts at: 5313 5362 5404 5507 5546 5930 6179 7039 7693
Size: 49 42 103 39 384 249 860 654
Cuts at: 7693 8145
Size: 452
Fragments arranged by size:
1208 860 654 599 452 414 384 359
330 267 253 249 233 210 202 172
162 139 129 111 108 103 94 92
66 66 49 46 42 39 26 24
3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 693 1923 2248 2588 2638 3424 3691 4240
Size: 693 1230 325 340 50 786 267 549
Cuts at: 4240 5861 8145
Size: 1621 2284
Fragments arranged by size:
2284 1621 1230 786 693 549 340 325
267 50
EcoO109I rG'GnC_Cy
Cuts at: 0 225 303 685 811 823 830 1513 1928
Size: 225 78 382 126 12 7 683 415
Cuts at: 1928 1944 2518 2572 2593 2706 2791 2828 2936
Size: 16 574 54 21 113 85 37 108
Cuts at: 2936 2973 2974 3157 3158 3187 3294 3295 3564
Size: 37 1 183 1 29 107 1 269
Cuts at: 3564 3594 3595 3841 4209 4220 4221 4233 4254
Size: 30 1 246 368 11 1 12 21
Cuts at: 4254 4333 4728 5454 5624 6054 6282 6305 6321
Size: 79 395 726 170 430 228 23 16
Cuts at: 6321 6367 6503 6504 6709 6999 7049 7104 7215
Size: 46 136 1 205 290 50 55 111
Cuts at: 7215 8145
Size: 930
Fragments arranged by size:
930 726 683 574 430 415 395 382
368 290 269 246 228 225 205 183
170 136 126 113 111 108 107 85
79 78 55 54 50 46 37 37
30 29 23 21 21 16 16 12
12 11 7 1 1 1 1 1
1
EcoRI G'AATT_C
Cuts at: 0 5336 8145
Size: 5336 2809
EcoRV GAT'ATC
Cuts at: 0 3261 8145
Size: 3261 4884
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 5055 5690 7575 8145
Size: 5055 635 1885 570
Fragments arranged by size:
5055 1885 635 570
FseI GG_CCGG'CC
Cuts at: 0 69 257 1413 8145
Size: 69 188 1156 6732
Fragments arranged by size:
6732 1156 188 69
FspI TGC'GCA
Cuts at: 0 2884 4071 4622 8145
Size: 2884 1187 551 3523
Fragments arranged by size:
3523 2884 1187 551
FspAI rTGC'GCAy
Cuts at: 0 2884 4071 8145
Size: 2884 1187 4074
Fragments arranged by size:
4074 2884 1187
HaeII r_GCGC'y
Cuts at: 0 80 445 2853 3234 3420 3810 3879 4365
Size: 80 365 2408 381 186 390 69 486
Cuts at: 4365 4946 5319 5502 5555 6152 6418 8120 8127
Size: 581 373 183 53 597 266 1702 7
Cuts at: 8127 8145
Size: 18
Fragments arranged by size:
2408 1702 597 581 486 390 381 373
365 266 186 183 80 69 53 18
7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 334 366 1254 1286 1790 1822 2187 2219
Size: 334 32 888 32 504 32 365 32
Cuts at: 2219 2507 2539 2710 2713 2742 2745 2920 2952
Size: 288 32 171 3 29 3 175 32
Cuts at: 2952 4756 4788 5932 5964 6064 6096 6313 6345
Size: 1804 32 1144 32 100 32 217 32
Cuts at: 6345 7174 7206 7733 7765 8145
Size: 829 32 527 32 380
Fragments arranged by size:
1804 1144 888 829 527 504 380 365
334 288 217 175 171 100 32 32
32 32 32 32 32 32 32 32
32 32 29 3 3
HincII GTy'rAC
Cuts at: 0 991 2946 4827 6582 7652 7838 8145
Size: 991 1955 1881 1755 1070 186 307
Fragments arranged by size:
1955 1881 1755 1070 991 307 186
KasI G'GCGC_C
Cuts at: 0 76 3416 3875 4942 6414 8116 8145
Size: 76 3340 459 1067 1472 1702 29
Fragments arranged by size:
3340 1702 1472 1067 459 76 29
MfeI C'AATT_G
Cuts at: 0 952 8145
Size: 952 7193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 1011 1513 2534 3295 3877 6717 8145
Size: 377 634 502 1021 761 582 2840 1428
Fragments arranged by size:
2840 1428 1021 761 634 582 502 377
MscI TGG'CCA
Cuts at: 0 4376 7687 8145
Size: 4376 3311 458
Fragments arranged by size:
4376 3311 458
MslI CAynn'nnrTG
Cuts at: 0 383 3583 3761 4423 4616 5185 5778 5941
Size: 383 3200 178 662 193 569 593 163
Cuts at: 5941 7406 7687 7934 7961 7993 8145
Size: 1465 281 247 27 32 152
Fragments arranged by size:
3200 1465 662 593 569 383 281 247
193 178 163 152 32 27
MspA1I CmG'CkG
Cuts at: 0 72 98 261 270 730 794 884 1177
Size: 72 26 163 9 460 64 90 293
Cuts at: 1177 1434 2065 2277 2352 2457 4199 6028 6180
Size: 257 631 212 75 105 1742 1829 152
Cuts at: 6180 6469 7030 8145
Size: 289 561 1115
Fragments arranged by size:
1829 1742 1115 631 561 460 293 289
257 212 163 152 105 90 75 72
64 26 9
NaeI GCC'GGC
Cuts at: 0 67 81 235 255 1411 3367 8145
Size: 67 14 154 20 1156 1956 4778
Fragments arranged by size:
4778 1956 1156 154 67 20 14
NarI GG'CG_CC
Cuts at: 0 77 3417 3876 4943 6415 8117 8145
Size: 77 3340 459 1067 1472 1702 28
Fragments arranged by size:
3340 1702 1472 1067 459 77 28
NcoI C'CATG_G
Cuts at: 0 2651 5353 6035 7518 7682 8145
Size: 2651 2702 682 1483 164 463
Fragments arranged by size:
2702 2651 1483 682 463 164
NgoMIV G'CCGG_C
Cuts at: 0 65 79 233 253 1409 3365 8145
Size: 65 14 154 20 1156 1956 4780
Fragments arranged by size:
4780 1956 1156 154 65 20 14
NsiI A_TGCA'T
Cuts at: 0 3822 7931 8145
Size: 3822 4109 214
Fragments arranged by size:
4109 3822 214
NspI r_CATG'y
Cuts at: 0 632 2611 4096 4891 7345 7569 8145
Size: 632 1979 1485 795 2454 224 576
Fragments arranged by size:
2454 1979 1485 795 632 576 224
PasI CC'CwG_GG
Cuts at: 0 2634 2794 3086 3184 5217 6499 6508 8145
Size: 2634 160 292 98 2033 1282 9 1637
Fragments arranged by size:
2634 2033 1637 1282 292 160 98 9
PciI A'CATG_T
Cuts at: 0 2607 7565 8145
Size: 2607 4958 580
Fragments arranged by size:
4958 2607 580
PflMI CCAn_nnn'nTGG
Cuts at: 0 826 3470 3642 4544 6692 6946 8145
Size: 826 2644 172 902 2148 254 1199
Fragments arranged by size:
2644 2148 1199 902 826 254 172
PfoI T'CCnGG_A
Cuts at: 0 819 1396 1921 2693 3794 5021 5063 5617
Size: 819 577 525 772 1101 1227 42 554
Cuts at: 5617 6008 6691 7055 8145
Size: 391 683 364 1090
Fragments arranged by size:
1227 1101 1090 819 772 683 577 554
525 391 364 42
PmlI CAC'GTG
Cuts at: 0 1753 7995 8145
Size: 1753 6242 150
Fragments arranged by size:
6242 1753 150
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2359 2391 3631 3663 4855 4887 7252 7284
Size: 2359 32 1240 32 1192 32 2365 32
Cuts at: 7284 8145
Size: 861
Fragments arranged by size:
2365 2359 1240 1192 861 32 32 32
32
PpuMI rG'GwC_Cy
Cuts at: 0 823 830 2572 2593 2706 2828 2936 4333
Size: 823 7 1742 21 113 122 108 1397
Cuts at: 4333 4728 5454 6282 6321 6367 6999 7104 8145
Size: 395 726 828 39 46 632 105 1041
Fragments arranged by size:
1742 1397 1041 828 823 726 632 395
122 113 108 105 46 39 21 7
PshAI GACnn'nnGTC
Cuts at: 0 954 4733 4822 8145
Size: 954 3779 89 3323
Fragments arranged by size:
3779 3323 954 89
PspOMI G'GGCC_C
Cuts at: 0 303 596 811 1943 2973 3157 3187 3294
Size: 303 293 215 1132 1030 184 30 107
Cuts at: 3294 3594 3841 4220 4278 5919 6054 6305 6503
Size: 300 247 379 58 1641 135 251 198
Cuts at: 6503 7048 8145
Size: 545 1097
Fragments arranged by size:
1641 1132 1097 1030 545 379 303 300
293 251 247 215 198 184 135 107
58 30
PstI C_TGCA'G
Cuts at: 0 694 2216 2225 2282 3215 3389 5394 6297
Size: 694 1522 9 57 933 174 2005 903
Cuts at: 6297 8145
Size: 1848
Fragments arranged by size:
2005 1848 1522 933 903 694 174 57
9
PvuII CAG'CTG
Cuts at: 0 730 794 1177 2065 2277 6180 6469 8145
Size: 730 64 383 888 212 3903 289 1676
Fragments arranged by size:
3903 1676 888 730 383 289 212 64
RsrII CG'GwC_CG
Cuts at: 0 4171 4455 8145
Size: 4171 284 3690
Fragments arranged by size:
4171 3690 284
SacI G_AGCT'C
Cuts at: 0 4267 4928 5493 7174 8057 8145
Size: 4267 661 565 1681 883 88
Fragments arranged by size:
4267 1681 883 661 565 88
SacII CC_GC'GG
Cuts at: 0 73 99 262 885 8145
Size: 73 26 163 623 7260
Fragments arranged by size:
7260 623 163 73 26
SanDI GG'GwC_CC
Cuts at: 0 830 2828 8145
Size: 830 1998 5317
Fragments arranged by size:
5317 1998 830
SapI GCTCTTCn'nnn_
Cuts at: 0 5263 5363 7175 8145
Size: 5263 100 1812 970
Fragments arranged by size:
5263 1812 970 100
SbfI CC_TGCA'GG
Cuts at: 0 694 2225 8145
Size: 694 1531 5920
Fragments arranged by size:
5920 1531 694
ScaI AGT'ACT
Cuts at: 0 4543 7810 8145
Size: 4543 3267 335
Fragments arranged by size:
4543 3267 335
SexAI A'CCwGG_T
Cuts at: 0 3727 8145
Size: 3727 4418
SfcI C'TryA_G
Cuts at: 0 690 1154 2212 2221 2278 3211 3385 5390
Size: 690 464 1058 9 57 933 174 2005
Cuts at: 5390 6293 7737 8145
Size: 903 1444 408
Fragments arranged by size:
2005 1444 1058 933 903 690 464 408
174 57 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 5358 8145
Size: 5358 2787
SfoI GGC'GCC
Cuts at: 0 78 3418 3877 4944 6416 8118 8145
Size: 78 3340 459 1067 1472 1702 27
Fragments arranged by size:
3340 1702 1472 1067 459 78 27
SgrAI Cr'CCGG_yG
Cuts at: 0 79 8145
Size: 79 8066
SmaI CCC'GGG
Cuts at: 0 33 62 183 1846 2647 3163 3291 3859
Size: 33 29 121 1663 801 516 128 568
Cuts at: 3859 5002 6059 8145
Size: 1143 1057 2086
Fragments arranged by size:
2086 1663 1143 1057 801 568 516 128
121 33 29
SmlI C'TyrA_G
Cuts at: 0 2386 3403 4876 7132 7705 8145
Size: 2386 1017 1473 2256 573 440
Fragments arranged by size:
2386 2256 1473 1017 573 440
SphI G_CATG'C
Cuts at: 0 4096 4891 7345 8145
Size: 4096 795 2454 800
Fragments arranged by size:
4096 2454 800 795
SrfI GCCC'GGGC
Cuts at: 0 2647 5002 8145
Size: 2647 2355 3143
Fragments arranged by size:
3143 2647 2355
StuI AGG'CCT
Cuts at: 0 2227 3112 6452 6916 7763 8145
Size: 2227 885 3340 464 847 382
Fragments arranged by size:
3340 2227 885 847 464 382
StyI C'CwwG_G
Cuts at: 0 959 2651 4268 4775 5353 5627 6035 6285
Size: 959 1692 1617 507 578 274 408 250
Cuts at: 6285 6360 6447 6528 7518 7682 8145
Size: 75 87 81 990 164 463
Fragments arranged by size:
1692 1617 990 959 578 507 463 408
274 250 164 87 81 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3197 3692 4000 4077 4312 4343 7720 8145
Size: 3197 495 308 77 235 31 3377 425
Fragments arranged by size:
3377 3197 495 425 308 235 77 31
TatI w'GTAC_w
Cuts at: 0 4021 4160 4541 4796 5288 7808 8145
Size: 4021 139 381 255 492 2520 337
Fragments arranged by size:
4021 2520 492 381 337 255 139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 528 560 1464 1496 3934 3966 7285 7317
Size: 528 32 904 32 2438 32 3319 32
Cuts at: 7317 8145
Size: 828
Fragments arranged by size:
3319 2438 904 828 528 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 284 2903 8145
Size: 284 2619 5242
Fragments arranged by size:
5242 2619 284
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1027 3792 3935 5517 7108 7725 8145
Size: 1027 2765 143 1582 1591 617 420
Fragments arranged by size:
2765 1591 1582 1027 617 420 143
XmaI C'CCGG_G
Cuts at: 0 31 60 181 1844 2645 3161 3289 3857
Size: 31 29 121 1663 801 516 128 568
Cuts at: 3857 5000 6057 8145
Size: 1143 1057 2088
Fragments arranged by size:
2088 1663 1143 1057 801 568 516 128
121 31 29
XmnI GAAnn'nnTTC
Cuts at: 0 2374 5038 8145
Size: 2374 2664 3107
Fragments arranged by size:
3107 2664 2374
ZraI GAC'GTC
Cuts at: 0 3945 7715 8145
Size: 3945 3770 430
Fragments arranged by size:
3945 3770 430
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflII AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtsI ClaI CspCI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HincII KasI
MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmlI
PpiI PpuMI PshAI PspOMI PstI PvuII RsrII SacI
SacII SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgrAI SmaI SmlI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AgeI AscI AseI AsiSI BaeI BmtI
BsiWI BspHI BssHII BstBI BtgZI DraI HindIII HpaI
KpnI MluI NdeI NheI NotI NruI PacI PmeI
PsiI PspXI PsrI PvuI SalI SgfI SnaBI SpeI
SspI SwaI XbaI XhoI