Restriction map for pF1KE2110
 (Linear) (Six-base) MAPSORT of: KIEE2110.seq  Check: 8801  from: 1  to: 2718



KIEE2110.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     456     978    2462    2718 
   Size:        456     522    1484     256

  Fragments arranged by size: 

                1484     522     456     256
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2178    2718 
   Size:       2178     540
 
AlwNI CAG_nnn'CTG

Cuts at:      0     556    1801    2718 
   Size:        556    1245     917

  Fragments arranged by size: 

                1245     917     556
 
ApaLI G'TGCA_C

Cuts at:      0    1200    2718 
   Size:       1200    1518
 
ApoI r'AATT_y

Cuts at:      0     360     429     562     718     847    2259    2680    2718 
   Size:        360      69     133     156     129    1412     421      38

  Fragments arranged by size: 

                1412     421     360     156     133     129      69      38
            
 
AvaI C'yCGr_G

Cuts at:      0      57    2290    2458    2718 
   Size:         57    2233     168     260

  Fragments arranged by size: 

                2233     260     168      57
 
AvrII C'CTAG_G

Cuts at:      0     439    1903    2718 
   Size:        439    1464     815

  Fragments arranged by size: 

                1464     815     439
 
BanI G'GyrC_C

Cuts at:      0    1423    1485    2220    2718 
   Size:       1423      62     735     498

  Fragments arranged by size: 

                1423     735     498      62
 
BanII G_rGCy'C

Cuts at:      0      25      58      83    1126    1184    1350    1889    2718 
   Size:         25      33      25    1043      58     166     539     829

  Fragments arranged by size: 

                1043     829     539     166      58      33      25      25
            
 
BbeI G_GCGC'C

Cuts at:      0    2224    2718 
   Size:       2224     494
 
BbvCI CC'TCA_GC

Cuts at:      0     127    2564    2718 
   Size:        127    2437     154

  Fragments arranged by size: 

                2437     154     127
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1923    2127    2718 
   Size:       1923     204     591

  Fragments arranged by size: 

                1923     591     204
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2124    2353    2718 
   Size:       2124     229     365

  Fragments arranged by size: 

                2124     365     229
 
BglII A'GATC_T

Cuts at:      0    1620    2718 
   Size:       1620    1098
 
BlpI GC'TnA_GC

Cuts at:      0     883    2718 
   Size:        883    1835
 
Bme1580I G_kGCm'C

Cuts at:      0    1204    2718 
   Size:       1204    1514
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     908    1140    2718 
   Size:        908     232    1578

  Fragments arranged by size: 

                1578     908     232
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     676     826     928    1625    1704    2556    2718 
   Size:        676     150     102     697      79     852     162

  Fragments arranged by size: 

                 852     697     676     162     150     102      79
 
Bpu10I CC'TnA_GC

Cuts at:      0     127     763    2564    2718 
   Size:        127     636    1801     154

  Fragments arranged by size: 

                1801     636     154     127
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1515    2718 
   Size:       1515    1203
 
BsaAI yAC'GTr

Cuts at:      0    2189    2718 
   Size:       2189     529
 
BsaBI GATnn'nnATC

Cuts at:      0    2420    2718 
   Size:       2420     298
 
BsaHI Gr'CG_yC

Cuts at:      0      92    1686    2221    2718 
   Size:         92    1594     535     497

  Fragments arranged by size: 

                1594     535     497      92
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1702    1732    2718 
   Size:       1702      30     986

  Fragments arranged by size: 

                1702     986      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     439    2308    2382    2718 
   Size:        439    1869      74     336

  Fragments arranged by size: 

                1869     439     336      74
 
BsiEI CG_ry'CG

Cuts at:      0    1441    2348    2718 
   Size:       1441     907     370

  Fragments arranged by size: 

                1441     907     370
 
BsiHKAI G_wGCw'C

Cuts at:      0      25    1204    1350    2718 
   Size:         25    1179     146    1368

  Fragments arranged by size: 

                1368    1179     146      25
 
Bsp1286I G_dGCh'C

Cuts at:      0      25      58      83    1126    1184    1204    1350    1889 
   Size:         25      33      25    1043      58      20     146     539

Cuts at:   1889    2718 
   Size:        829

  Fragments arranged by size: 

                1043     829     539     146      58      33      25      25
                  20
 
BspHI T'CATG_A

Cuts at:      0      83    1403    2591    2718 
   Size:         83    1320    1188     127

  Fragments arranged by size: 

                1320    1188     127      83
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     266     700    2718 
   Size:        266     434    2018

  Fragments arranged by size: 

                2018     434     266
 
BsrDI GCAATG_nn'

Cuts at:      0     702    1594    1867    2077    2204    2636    2718 
   Size:        702     892     273     210     127     432      82

  Fragments arranged by size: 

                 892     702     432     273     210     127      82
 
BsrFI r'CCGG_y

Cuts at:      0       4    1646    1847    2343    2718 
   Size:          4    1642     201     496     375

  Fragments arranged by size: 

                1642     496     375     201       4
 
BsrGI T'GTAC_A

Cuts at:      0    1699    2718 
   Size:       1699    1019
 
BssSI C'ACGA_G

Cuts at:      0    2150    2718 
   Size:       2150     568
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     556    2275    2718 
   Size:        556    1719     443

  Fragments arranged by size: 

                1719     556     443
 
BstYI r'GATC_y

Cuts at:      0    1293    1620    2718 
   Size:       1293     327    1098

  Fragments arranged by size: 

                1293    1098     327
 
BtgI C'CryG_G

Cuts at:      0     662    1457    2482    2718 
   Size:        662     795    1025     236

  Fragments arranged by size: 

                1025     795     662     236
 
EaeI y'GGCC_r

Cuts at:      0       2    1438    1648    1777    2264    2345    2660    2718 
   Size:          2    1436     210     129     487      81     315      58

  Fragments arranged by size: 

                1436     487     315     210     129      81      58       2
            
 
EagI C'GGCC_G

Cuts at:      0    1438    2345    2718 
   Size:       1438     907     373

  Fragments arranged by size: 

                1438     907     373
 
EarI CTCTTCn'nnn_

Cuts at:      0      13     400     601     976    1405    1827    2045    2078 
   Size:         13     387     201     375     429     422     218      33

Cuts at:   2078    2115    2718 
   Size:         37     603

  Fragments arranged by size: 

                 603     429     422     387     375     218     201      37
                  33      13
 
EcoICRI GAG'CTC

Cuts at:      0      23    1348    2718 
   Size:         23    1325    1370

  Fragments arranged by size: 

                1370    1325      23
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     456     676     826     928     978    1625    1704    2462 
   Size:        456     220     150     102      50     647      79     758

Cuts at:   2462    2556    2718 
   Size:         94     162

  Fragments arranged by size: 

                 758     647     456     220     162     150     102      94
                  79      50
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     397    2112    2718 
   Size:        397    1715     606

  Fragments arranged by size: 

                1715     606     397
 
EcoRI G'AATT_C

Cuts at:      0     429    2718 
   Size:        429    2289
 
EcoRV GAT'ATC

Cuts at:      0    2376    2718 
   Size:       2376     342
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     111    1492    2718 
   Size:        111    1381    1226

  Fragments arranged by size: 

                1381    1226     111
 
HaeII r_GCGC'y

Cuts at:      0     229    2224    2718 
   Size:        229    1995     494

  Fragments arranged by size: 

                1995     494     229
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     939     971    1702    1734    2718 
   Size:        939      32     731      32     984

  Fragments arranged by size: 

                 984     939     731      32      32
 
HindIII A'AGCT_T

Cuts at:      0     575    1469    2627    2718 
   Size:        575     894    1158      91

  Fragments arranged by size: 

                1158     894     575      91
 
KasI G'GCGC_C

Cuts at:      0    2220    2718 
   Size:       2220     498
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1158    1641    2718 
   Size:       1158     483    1077

  Fragments arranged by size: 

                1158    1077     483
 
MscI TGG'CCA

Cuts at:      0    2662    2718 
   Size:       2662      56
 
MslI CAynn'nnrTG

Cuts at:      0    1942    2154    2656    2718 
   Size:       1942     212     502      62

  Fragments arranged by size: 

                1942     502     212      62
 
MspA1I CmG'CkG

Cuts at:      0      44    2484    2516    2718 
   Size:         44    2440      32     202

  Fragments arranged by size: 

                2440     202      44      32
 
NaeI GCC'GGC

Cuts at:      0       6    2345    2718 
   Size:          6    2339     373

  Fragments arranged by size: 

                2339     373       6
 
NarI GG'CG_CC

Cuts at:      0    2221    2718 
   Size:       2221     497
 
NcoI C'CATG_G

Cuts at:      0     662    1457    2718 
   Size:        662     795    1261

  Fragments arranged by size: 

                1261     795     662
 
NgoMIV G'CCGG_C

Cuts at:      0       4    2343    2718 
   Size:          4    2339     375

  Fragments arranged by size: 

                2339     375       4
 
NspI r_CATG'y

Cuts at:      0     483    2159    2718 
   Size:        483    1676     559

  Fragments arranged by size: 

                1676     559     483
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     483    2718 
   Size:        483    2235
 
PfoI T'CCnGG_A

Cuts at:      0    2675    2718 
   Size:       2675      43
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1508    1540    2718 
   Size:       1508      32    1178

  Fragments arranged by size: 

                1508    1178      32
 
PshAI GACnn'nnGTC

Cuts at:      0     595    2718 
   Size:        595    2123
 
PvuII CAG'CTG

Cuts at:      0    2516    2718 
   Size:       2516     202
 
SacI G_AGCT'C

Cuts at:      0      25    1350    2718 
   Size:         25    1325    1368

  Fragments arranged by size: 

                1368    1325      25
 
SacII CC_GC'GG

Cuts at:      0    2485    2718 
   Size:       2485     233
 
SapI GCTCTTCn'nnn_

Cuts at:      0      13     601    2718 
   Size:         13     588    2117

  Fragments arranged by size: 

                2117     588      13
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2124    2353    2718 
   Size:       2124     229     365

  Fragments arranged by size: 

                2124     365     229
 
SfoI GGC'GCC

Cuts at:      0    2222    2718 
   Size:       2222     496
 
SmlI C'TyrA_G

Cuts at:      0    1530    2718 
   Size:       1530    1188
 
SnaBI TAC'GTA

Cuts at:      0    2189    2718 
   Size:       2189     529
 
SspI AAT'ATT

Cuts at:      0    2208    2718 
   Size:       2208     510
 
StuI AGG'CCT

Cuts at:      0    2118    2466    2718 
   Size:       2118     348     252

  Fragments arranged by size: 

                2118     348     252
 
StyI C'CwwG_G

Cuts at:      0     439     601     662    1427    1457    1755    1903    2718 
   Size:        439     162      61     765      30     298     148     815

  Fragments arranged by size: 

                 815     765     439     298     162     148      61      30
            
 
TatI w'GTAC_w

Cuts at:      0     889    1699    2096    2718 
   Size:        889     810     397     622

  Fragments arranged by size: 

                 889     810     622     397
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2022    2281    2718 
   Size:       2022     259     437

  Fragments arranged by size: 

                2022     437     259
 
XmnI GAAnn'nnTTC

Cuts at:      0    1554    2718 
   Size:       1554    1164
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI    ApaLI     ApoI     AvaI    AvrII     BanI
    BanII     BbeI    BbvCI    BciVI     BglI    BglII     BlpI Bme1580I
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI
    BseRI    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI    BstYI     BtgI     EaeI     EagI     EarI
  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI    HaeII    Hin4I
  HindIII     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI    PflMI     PfoI     PpiI    PshAI    PvuII
     SacI    SacII     SapI     SfiI     SfoI     SmlI    SnaBI     SspI
     StuI     StyI     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI     AscI     AseI    AsiSI
     BaeI    BamHI     BbsI     BcgI     BclI    BfrBI    BmgBI     BmrI
     BmtI     BsaI    BsaWI    BseYI     BsgI    BsiWI     BsmI    BsmBI
    BspEI    BsrBI   BssHII    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EciI
 EcoO109I     FseI     FspI    FspAI   HincII     HpaI     KpnI     MfeI
     MluI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PmeI     PmlI    PpuMI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SalI    SanDI     SbfI     ScaI    SexAI
     SfcI     SgfI    SgrAI     SmaI     SpeI     SphI     SrfI     SwaI
    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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