Restriction map for pF1KE4276
##################################
# NO CUT ENZYMES (  103 enzymes) #
##################################

Acc65I      AccII       AgeI        AhdI        AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AspEI       AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BcgI        BcnI        
BglI        BlnI        BlpI        Bpu1102I    BsaAI       
BsiEI       BsiWI       BspDI       BspHI       BsrBI       
BsrFI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     FseI        
FspI        HaeII       HhaI        HinP1I      KasI        
KpnI        KspI        MluI        MspA1I      MvnI        
NaeI        NarI        NciI        NcoI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspI        
StuI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AatII        2221  GACGTC               GACGT^C
AccI         2102  GTCTAC               GT^[AC][GT]AC
AclI         2759  AACGTT               AA^CGTT
AcyI         2221  GACGTC               G[GA]^CG[CT]C
AflII          99  CTTAAG               C^TTAAG
Alw44I       3299  GTGCAC               G^TGCAC
ApaLI        3299  GTGCAC               G^TGCAC
AspI         2540  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          497  CTCGAG               C^[CT]CG[GA]G
BalI         2365  TGGCCA               TGG^CCA
BanII          88  GGGCTC               G[GA]GC[CT]^C
BbvCI        3557  CCTCAGC              CCTCAGC(-5/-2)
BciVI        2048  GTATCC               GTATCC(6/5)
BfrI           99  CTTAAG               C^TTAAG
BsaHI        2221  GACGTC               G[GA]^CG[CT]C
BsmBI        3420  GAGACG               CGTCTC(1/5)
BsoBI         497  CTCGAG               C^[CT]CG[GA]G
Bsp1407I     2276  TGTACA               T^GTACA
BspMI        1485  ACCTGC               ACCTGC(4/8)
BsrDI         467  GCAATG               GCAATG(2/0)
BsrGI        2276  TGTACA               T^GTACA
BssSI        3601  CTCGTG               CACGAG(-5/-1)
DsaI         3594  CCACGG               C^C[GA][CT]GG
EaeI         2365  TGGCCA               [CT]^GGCC[GA]
FauI         2478  GCGGG                CCCGC(4/6)
HgaI         3178  GACGC                GACGC(5/10)
Hin1I        2221  GACGTC               G[GA]^CG[CT]C
HpaI         1288  GTTAAC               GTT^AAC
MluNI        2365  TGGCCA               TGG^CCA
MscI         2365  TGGCCA               TGG^CCA
PaeR7I        497  CTCGAG               C^TCGAG
PflFI        2540  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1711  CCATCCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I     2759  AACGTT               AA^CGTT
SapI          796  GCTCTTC              GCTCTTC(1/4)
SphI          995  GCATGC               GCATG^C
SspBI        2276  TGTACA               T^GTACA
SwaI         1454  ATTTAAAT             ATTT^AAAT
Tth111I      2540  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1711  CCATCCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          707  CCACTAGAAATATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          497  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccIII        123  TCCGGA               T^CCGGA
             1241  TCCGGA              
AciI         1073  GCGG                 CCGC(-3/-1)
             2478  GCGG                
AflIII        170  ACATGT               A^C[GA][CT]GT
             2313  ACATGT              
BclI         2706  TGATCA               T^GATCA
             3287  TGATCA              
BglII        1651  AGATCT               A^GATCT
             2651  AGATCT              
BsaBI        3583  GATCACGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3700  GATGTGAATC          
BsaI         3720  GAGACC               GGTCTC(1/5)
             3598  GGTCTC              
BsaWI         123  TCCGGA               [AT]^CCGG[AT]
             1241  TCCGGA              
BseAI         123  TCCGGA               T^CCGGA
             1241  TCCGGA              
BsiYI         874  CCTCTACTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1711  CCATCCAGTGG         
BslI          874  CCTCTACTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1711  CCATCCAGTGG         
BsmFI        3344  GGGAC                GGGAC(10/14)
             2664  GTCCC               
BspEI         123  TCCGGA               T^CCGGA
             1241  TCCGGA              
BspLU11I      170  ACATGT               A^CATGT
             2313  ACATGT              
BstAPI        961  GCAGAGAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1305  GCAGCATCTGC         
BstXI        2516  CCAAAAACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3333  CCAGATTAGTGG        
DraII        1575  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             2662  GGGTCCC             
EcoO109I     1575  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             2662  GGGTCCC             
EcoT22I       993  ATGCAT               ATGCA^T
             2891  ATGCAT              
FbaI         2706  TGATCA               T^GATCA
             3287  TGATCA              
HapII         124  CCGG                 C^CGG
             1242  CCGG                
HindIII       685  AAGCTT               A^AGCTT
             2159  AAGCTT              
HpaII         124  CCGG                 C^CGG
             1242  CCGG                
MamI         3583  GATCACGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3700  GATGTGAATC          
MfeI          839  CAATTG               C^AATTG
             2425  CAATTG              
MroI          123  TCCGGA               T^CCGGA
             1241  TCCGGA              
MslI         1887  CATTGTAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2314  CATGTAAATG          
MspI          124  CCGG                 C^CGG
             1242  CCGG                
MunI          839  CAATTG               C^AATTG
             2425  CAATTG              
NdeI          629  CATATG               CA^TATG
             3380  CATATG              
NsiI          993  ATGCAT               ATGCA^T
             2891  ATGCAT              
PpuMI        1575  AGGTCCT              [GA]G^G[AT]CC[CT]
             2662  GGGTCCC             


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        AlwNI       
ApoI        Asp700I     AspHI       AvaII       BbsI        
BbvI        BfaI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsgI        BsiHKAI     BsmAI       
BsmI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraI        EarI        EcoRI       EcoRII      
EcoRV       EcoT14I     Fnu4HI      FokI        HaeIII      
HincII      HindII      HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XbaI        XhoII       
XmnI        
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