Restriction map for pF1KE4276
 (Linear) (Six-base) MAPSORT of: KIEE4276.seq  Check: 7004  from: 1  to: 3783



KIEE4276.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AatII G_ACGT'C

Cuts at:      0    2225    3783 
   Size:       2225    1558
 
AccI GT'mk_AC

Cuts at:      0    2103    3783 
   Size:       2103    1680
 
AclI AA'CG_TT

Cuts at:      0    2760    3783 
   Size:       2760    1023
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1439    1457    1754    2792    3413    3783 
   Size:       1439      18     297    1038     621     370

  Fragments arranged by size: 

                1439    1038     621     370     297      18
 
AflII C'TTAA_G

Cuts at:      0      99    3783 
   Size:         99    3684
 
AflIII A'CryG_T

Cuts at:      0     170    2313    3783 
   Size:        170    2143    1470

  Fragments arranged by size: 

                2143    1470     170
 
AlwNI CAG_nnn'CTG

Cuts at:      0     783    1311    1777    3783 
   Size:        783     528     466    2006

  Fragments arranged by size: 

                2006     783     528     466
 
ApaLI G'TGCA_C

Cuts at:      0    3299    3783 
   Size:       3299     484
 
ApoI r'AATT_y

Cuts at:      0     561     755     862    1226    1393    1410    1953    2014 
   Size:        561     194     107     364     167      17     543      61

Cuts at:   2014    2111    2197    2284    2393    2439    2825    3126    3202 
   Size:         97      86      87     109      46     386     301      76

Cuts at:   3202    3236    3767    3783 
   Size:         34     531      16

  Fragments arranged by size: 

                 561     543     531     386     364     301     194     167
                 109     107      97      87      86      76      61      46
                  34      17      16
 
AvaI C'yCGr_G

Cuts at:      0     497    3783 
   Size:        497    3286
 
BanII G_rGCy'C

Cuts at:      0      92    3783 
   Size:         92    3691
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1022    1055    2360    2443    3419    3680    3758    3783 
   Size:       1022      33    1305      83     976     261      78      25

  Fragments arranged by size: 

                1305    1022     976     261      83      78      33      25
            
 
BbvCI CC'TCA_GC

Cuts at:      0    3558    3783 
   Size:       3558     225
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2059    3783 
   Size:       2059    1724
 
BclI T'GATC_A

Cuts at:      0    2706    3287    3783 
   Size:       2706     581     496

  Fragments arranged by size: 

                2706     581     496
 
BfrBI ATG'CAT

Cuts at:      0     995    2893    3783 
   Size:        995    1898     890

  Fragments arranged by size: 

                1898     995     890
 
BglII A'GATC_T

Cuts at:      0    1651    2651    3783 
   Size:       1651    1000    1132

  Fragments arranged by size: 

                1651    1132    1000
 
Bme1580I G_kGCm'C

Cuts at:      0    3303    3783 
   Size:       3303     480
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    3290    3365    3783 
   Size:       3290      75     418

  Fragments arranged by size: 

                3290     418      75
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3044    3783 
   Size:       3044     739
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     835    1652    3289    3316    3783 
   Size:        835     817    1637      27     467

  Fragments arranged by size: 

                1637     835     817     467      27
 
Bpu10I CC'TnA_GC

Cuts at:      0    2975    3558    3783 
   Size:       2975     583     225

  Fragments arranged by size: 

                2975     583     225
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76    3783 
   Size:         76    3707
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3604    3714    3783 
   Size:       3604     110      69

  Fragments arranged by size: 

                3604     110      69
 
BsaBI GATnn'nnATC

Cuts at:      0    3587    3704    3783 
   Size:       3587     117      79

  Fragments arranged by size: 

                3587     117      79
 
BsaHI Gr'CG_yC

Cuts at:      0    2222    3783 
   Size:       2222    1561
 
BsaWI w'CCGG_w

Cuts at:      0     123    1241    3783 
   Size:        123    1118    2542

  Fragments arranged by size: 

                2542    1118     123
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1045    1075    2556    2586    3783 
   Size:       1045      30    1481      30    1197

  Fragments arranged by size: 

                1481    1197    1045      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1066    1141    2577    3553    3697    3783 
   Size:       1066      75    1436     976     144      86

  Fragments arranged by size: 

                1436    1066     976     144      86      75
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     889    1064    1583    3783 
   Size:        889     175     519    2200

  Fragments arranged by size: 

                2200     889     519     175
 
BsiHKAI G_wGCw'C

Cuts at:      0    1630    3303    3462    3783 
   Size:       1630    1673     159     321

  Fragments arranged by size: 

                1673    1630     321     159
 
BsmI GAATG_Cn'

Cuts at:      0     972    2180    3743    3783 
   Size:        972    1208    1563      40

  Fragments arranged by size: 

                1563    1208     972      40
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3414    3783 
   Size:       3414     369
 
Bsp1286I G_dGCh'C

Cuts at:      0      92    1630    3303    3462    3783 
   Size:         92    1538    1673     159     321

  Fragments arranged by size: 

                1673    1538     321     159      92
 
BspEI T'CCGG_A

Cuts at:      0     123    1241    3783 
   Size:        123    1118    2542

  Fragments arranged by size: 

                2542    1118     123
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1494    3783 
   Size:       1494    2289
 
BsrDI GCAATG_nn'

Cuts at:      0     474    3783 
   Size:        474    3309
 
BsrGI T'GTAC_A

Cuts at:      0    2276    3783 
   Size:       2276    1507
 
BssSI C'ACGA_G

Cuts at:      0    3601    3783 
   Size:       3601     182
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     967    1311    3783 
   Size:        967     344    2472

  Fragments arranged by size: 

                2472     967     344
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2523    3340    3783 
   Size:       2523     817     443

  Fragments arranged by size: 

                2523     817     443
 
BstYI r'GATC_y

Cuts at:      0    1651    1855    2306    2651    3783 
   Size:       1651     204     451     345    1132

  Fragments arranged by size: 

                1651    1132     451     345     204
 
BtgI C'CryG_G

Cuts at:      0    3594    3783 
   Size:       3594     189
 
BtsI GCAGTG_nn'

Cuts at:      0     323     772    3469    3572    3783 
   Size:        323     449    2697     103     211

  Fragments arranged by size: 

                2697     449     323     211     103
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2996    3031    3783 
   Size:       2996      35     752

  Fragments arranged by size: 

                2996     752      35
 
DraI TTT'AAA

Cuts at:      0     213     257    1457    3783 
   Size:        213      44    1200    2326

  Fragments arranged by size: 

                2326    1200     213      44
 
EaeI y'GGCC_r

Cuts at:      0    2365    3783 
   Size:       2365    1418
 
EarI CTCTTCn'nnn_

Cuts at:      0     388     803    1043    1118    1300    1535    1910    3783 
   Size:        388     415     240      75     182     235     375    1873

  Fragments arranged by size: 

                1873     415     388     375     240     235     182      75
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1088    3783 
   Size:       1088    2695
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     835    1439    1457    1652    1754    2792    3289    3316 
   Size:        835     604      18     195     102    1038     497      27

Cuts at:   3316    3413    3783 
   Size:         97     370

  Fragments arranged by size: 

                1038     835     604     497     370     195     102      97
                  27      18
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1576    2663    3783 
   Size:       1576    1087    1120

  Fragments arranged by size: 

                1576    1120    1087
 
EcoRI G'AATT_C

Cuts at:      0     755    2014    2393    3783 
   Size:        755    1259     379    1390

  Fragments arranged by size: 

                1390    1259     755     379
 
EcoRV GAT'ATC

Cuts at:      0     153    2214    2799    3783 
   Size:        153    2061     585     984

  Fragments arranged by size: 

                2061     984     585     153
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     708    3783 
   Size:        708    3075
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1045    1077    1537    1569    1651    1683    2531    2563 
   Size:       1045      32     460      32      82      32     848      32

Cuts at:   2563    2923    2955    3409    3441    3619    3651    3783 
   Size:        360      32     454      32     178      32     132

  Fragments arranged by size: 

                1045     848     460     454     360     178     132      82
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0      55    1290    3005    3664    3783 
   Size:         55    1235    1715     659     119

  Fragments arranged by size: 

                1715    1235     659     119      55
 
HindIII A'AGCT_T

Cuts at:      0     685    2159    3783 
   Size:        685    1474    1624

  Fragments arranged by size: 

                1624    1474     685
 
HpaI GTT'AAC

Cuts at:      0    1290    3783 
   Size:       1290    2493
 
MfeI C'AATT_G

Cuts at:      0     839    2425    3783 
   Size:        839    1586    1358

  Fragments arranged by size: 

                1586    1358     839
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     131    1100    2334    3783 
   Size:        131     969    1234    1449

  Fragments arranged by size: 

                1449    1234     969     131
 
MscI TGG'CCA

Cuts at:      0    2367    3783 
   Size:       2367    1416
 
MslI CAynn'nnrTG

Cuts at:      0    1891    2318    3783 
   Size:       1891     427    1465

  Fragments arranged by size: 

                1891    1465     427
 
NdeI CA'TA_TG

Cuts at:      0     630    3381    3783 
   Size:        630    2751     402

  Fragments arranged by size: 

                2751     630     402
 
NsiI A_TGCA'T

Cuts at:      0     997    2895    3783 
   Size:        997    1898     888

  Fragments arranged by size: 

                1898     997     888
 
NspI r_CATG'y

Cuts at:      0     174     999    1305    2317    2897    3511    3783 
   Size:        174     825     306    1012     580     614     272

  Fragments arranged by size: 

                1012     825     614     580     306     272     174
 
PciI A'CATG_T

Cuts at:      0     170    2313    3783 
   Size:        170    2143    1470

  Fragments arranged by size: 

                2143    1470     170
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1717    3783 
   Size:       1717    2066
 
PfoI T'CCnGG_A

Cuts at:      0     386    1677    1986    3305    3783 
   Size:        386    1291     309    1319     478

  Fragments arranged by size: 

                1319    1291     478     386     309
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     592     624    3783 
   Size:        592      32    3159

  Fragments arranged by size: 

                3159     592      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1576    2663    3783 
   Size:       1576    1087    1120

  Fragments arranged by size: 

                1576    1120    1087
 
PsiI TTA'TAA

Cuts at:      0    1588    3500    3783 
   Size:       1588    1912     283

  Fragments arranged by size: 

                1912    1588     283
 
PstI C_TGCA'G

Cuts at:      0     315     779    1316    1737    3476    3783 
   Size:        315     464     537     421    1739     307

  Fragments arranged by size: 

                1739     537     464     421     315     307
 
SanDI GG'GwC_CC

Cuts at:      0    2663    3783 
   Size:       2663    1120
 
SapI GCTCTTCn'nnn_

Cuts at:      0     803    3783 
   Size:        803    2980
 
SfcI C'TryA_G

Cuts at:      0     311     775    1312    1733    2104    3062    3229    3472 
   Size:        311     464     537     421     371     958     167     243

Cuts at:   3472    3783 
   Size:        311

  Fragments arranged by size: 

                 958     537     464     421     371     311     311     243
                 167
 
SmlI C'TyrA_G

Cuts at:      0      91      99     497    3783 
   Size:         91       8     398    3286

  Fragments arranged by size: 

                3286     398      91       8
 
SphI G_CATG'C

Cuts at:      0     999    3783 
   Size:        999    2784
 
StyI C'CwwG_G

Cuts at:      0    1791    1899    2557    3031    3724    3783 
   Size:       1791     108     658     474     693      59

  Fragments arranged by size: 

                1791     693     658     474     108      59
 
SwaI ATTT'AAAT

Cuts at:      0    1457    3783 
   Size:       1457    2326
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3477    3783 
   Size:       3477     306
 
TatI w'GTAC_w

Cuts at:      0    1385    2276    2466    3783 
   Size:       1385     891     190    1317

  Fragments arranged by size: 

                1385    1317     891     190
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1238    1270    1599    1631    2556    2588    3783 
   Size:       1238      32     329      32     925      32    1195

  Fragments arranged by size: 

                1238    1195     925     329      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2543    3783 
   Size:       2543    1240
 
XbaI T'CTAG_A

Cuts at:      0     234    1206    1648    1743    2922    3783 
   Size:        234     972     442      95    1179     861

  Fragments arranged by size: 

                1179     972     861     442     234      95
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     714    3783 
   Size:        714    3069
 
XhoI C'TCGA_G

Cuts at:      0     497    3783 
   Size:        497    3286
 
XmnI GAAnn'nnTTC

Cuts at:      0     221     564    2653    3783 
   Size:        221     343    2089    1130

  Fragments arranged by size: 

                2089    1130     343     221
 
ZraI GAC'GTC

Cuts at:      0    2223    3783 
   Size:       2223    1560
 

 Enzymes that do cut:

    AatII     AccI     AclI     AcuI    AflII   AflIII    AlwNI    ApaLI
     ApoI     AvaI    BanII     BbsI    BbvCI    BciVI     BclI    BfrBI
    BglII Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspMI    BsrDI    BsrGI    BssSI   BstAPI
    BstXI    BstYI     BtgI     BtsI    CspCI     DraI     EaeI     EarI
     EciI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI    Hin4I   HincII
  HindIII     HpaI     MfeI     MmeI     MscI     MslI     NdeI     NsiI
     NspI     PciI    PflMI     PfoI     PpiI    PpuMI     PsiI     PstI
    SanDI     SapI     SfcI     SmlI     SphI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AfeI     AgeI     AhdI     AleI     AlfI     AloI
     ApaI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI     BcgI     BglI     BlpI    BmgBI     BmtI    BsaAI    BseYI
    BsiEI    BsiWI    BspHI    BsrBI    BsrFI   BssHII    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI   DraIII     DrdI     EagI  EcoICRI
    EcoNI     FseI     FspI    FspAI    HaeII     KasI     KpnI     MluI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     PacI     PasI     PmeI     PmlI    PshAI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     StuI     XmaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com