Restriction map for pF1KE3466
##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AocI        Aor51HI     AscI        AseI        
AsnI        Asp718I     AspI        AviII       AvrII       
BbeI        BbrPI       BbvCI       BcgI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaHI       BseAI       BsiWI       BsmBI       BspEI       
Bst1107I    BstEII      BstPI       BstZ17I     Bsu36I      
CelII       CpoI        DraIII      EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoT22I     
FseI        FspI        Hin1I       HindIII     HpaI        
KasI        KpnI        MluI        MroI        NaeI        
NarI        NcoI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    RsrII       SacI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SmlI        SnaBI       SphI        Sse8387I    SspI        
StuI        SwaI        Tsp45I      Tth111I     XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AgeI          569  ACCGGT               A^CCGGT
AhdI          457  GACATACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1715  GTGCAC               G^TGCAC
ApaLI        1715  GTGCAC               G^TGCAC
AspEI         457  GACATACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         2316  CCCGAG               C^[CT]CG[GA]G
BalI         1926  TGGCCA               TGG^CCA
BclI          381  TGATCA               T^GATCA
BcnI         4004  CCCGG                CC^[GC]GG
BsaBI        1240  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         569  ACCGGT               [AT]^CCGG[AT]
BsiEI        1639  CGATCG               CG[GA][CT]^CG
BsoBI        2316  CCCGAG               C^[CT]CG[GA]G
BspDI        1073  ATCGAT               AT^CGAT
BsrBI        1656  GAGCGG               CCGCTC(-3/-3)
BsrFI         569  ACCGGT               [GA]^CCGG[CT]
BssHII       1231  GCGCGC               G^CGCGC
BssSI          34  CTCGTG               CACGAG(-5/-1)
BstAPI       1577  GCAGTCAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        3736  CCAGTGAAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        569  ACCGGT               [GA]^CCGG[CT]
ClaI         1073  ATCGAT               AT^CGAT
EaeI         1926  TGGCCA               [CT]^GGCC[GA]
Eam1105I      457  GACATACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         231  CCTTCAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          381  TGATCA               T^GATCA
HaeII         532  AGCGCC               [GA]GCGC^[CT]
HgaI          404  GCGTC                GACGC(5/10)
KspI          140  CCGCGG               CCGC^GG
MaeIII       2210  GTTAC                ^GT[ACGT]AC
MamI         1240  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2072  CAATTG               C^AATTG
MluNI        1926  TGGCCA               TGG^CCA
MscI         1926  TGGCCA               TGG^CCA
MunI         2072  CAATTG               C^AATTG
NciI         4004  CCCGG                CC^[GC]GG
NdeI         1746  CATATG               CA^TATG
PflMI        1540  CCATTGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         569  ACCGGT               A^CCGGT
PpuMI         415  GGGACCT              [GA]G^G[AT]CC[CT]
PvuI         1639  CGATCG               CGAT^CG
PvuII        2422  CAGCTG               CAG^CTG
SacII         140  CCGCGG               CCGC^GG
SalI          364  GTCGAC               G^TCGAC
SapI          499  GAAGAGC              GCTCTTC(1/4)
SpeI         3106  ACTAGT               A^CTAGT
Van91I       1540  CCATTGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         4147  CCAGCCTCCTTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI          364  GTCGAC               GT^[AC][GT]AC
             3001  GTAGAC              
AlwNI         837  CAGGTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1052  CAGATTCTG           
ApaI          977  GGGCCC               GGGCC^C
             3214  GGGCCC              
Asp700I       226  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2899  GAATTAGTTC          
BamHI        1965  GGATCC               G^GATCC
             2474  GGATCC              
BciVI        3075  GTATCC               GTATCC(6/5)
             4092  GTATCC              
BsaI         1343  GAGACC               GGTCTC(1/5)
             3452  GGTCTC              
Bsp1407I     2505  TGTACA               T^GTACA
             3708  TGTACA              
BspHI        1179  TCATGA               T^CATGA
             3389  TCATGA              
BsrGI        2505  TGTACA               T^GTACA
             3708  TGTACA              
BstBI        3494  TTCGAA               TT^CGAA
             3506  TTCGAA              
DrdI          687  GACGGACCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3084  GACAGACATGTC        
DsaI          140  CCGCGG               C^C[GA][CT]GG
             1929  CCACGG              
EarI          499  GAAGAG               CTCTTC(1/4)
             3254  GAAGAG              
EcoRI        2055  GAATTC               G^AATTC
             2301  GAATTC              
EcoRV         639  GATATC               GAT^ATC
             4008  GATATC              
FauI           10  CCCGC                CCCGC(4/6)
             1658  GCGGG               
HapII         570  CCGG                 C^CGG
             4005  CCGG                
HpaII         570  CCGG                 C^CGG
             4005  CCGG                
Ksp632I       499  GAAGAG               CTCTTC(1/4)
             3254  GAAGAG              
MaeII        3857  ACGT                 A^CGT
             4073  ACGT                
MspI          570  CCGG                 C^CGG
             4005  CCGG                
NspV         3494  TTCGAA               TT^CGAA
             3506  TTCGAA              
RcaI         1179  TCATGA               T^CATGA
             3389  TCATGA              
SfuI         3494  TTCGAA               TT^CGAA
             3506  TTCGAA              
SspBI        2505  TGTACA               T^GTACA
             3708  TGTACA              
XbaI          804  TCTAGA               T^CTAGA
             2059  TCTAGA              
XmnI          226  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2899  GAATTAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        ApoI        AspHI       AvaII       
BanI        BanII       BbsI        BbvI        BfaI        
BglII       BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    BspLU11I    
BspMI       BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HhaI        HinP1I      HincII      HindII      
HinfI       HphI        ItaI        MaeI        MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MvaI        MvnI        MwoI        NdeII       
NlaIII      NlaIV       NspI        PleI        PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       TthHB8I     XhoII       
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