Restriction map for pF1KE3466
 (Linear) (Six-base) MAPSORT of: KIEE3466.seq  Check: 8423  from: 1  to: 4170



KIEE3466.orf

 With 182 enzymes: SgfI * 

                        August 1, 2019 16:47  ..

AccI GT'mk_AC

Cuts at:      0     365    3002    4170 
   Size:        365    2637    1168

  Fragments arranged by size: 

                2637    1168     365
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     934    2190    2621    2687    4170 
   Size:        217     717    1256     431      66    1483

  Fragments arranged by size: 

                1483    1256     717     431     217      66
 
AflIII A'CryG_T

Cuts at:      0     429    2086    3089    3683    4170 
   Size:        429    1657    1003     594     487

  Fragments arranged by size: 

                1657    1003     594     487     429
 
AgeI A'CCGG_T

Cuts at:      0     569    4170 
   Size:        569    3601
 
AhdI GACnn_n'nnGTC

Cuts at:      0     462    4170 
   Size:        462    3708
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      52    4170 
   Size:         52    4118
 
AlwNI CAG_nnn'CTG

Cuts at:      0     842    1057    4170 
   Size:        842     215    3113

  Fragments arranged by size: 

                3113     842     215
 
ApaI G_GGCC'C

Cuts at:      0     981    3218    4170 
   Size:        981    2237     952

  Fragments arranged by size: 

                2237     981     952
 
ApaLI G'TGCA_C

Cuts at:      0    1715    4170 
   Size:       1715    2455
 
ApoI r'AATT_y

Cuts at:      0     603    1212    1431    1783    2055    2237    2301    2787 
   Size:        603     609     219     352     272     182      64     486

Cuts at:   2787    3311    3498    3643    4170 
   Size:        524     187     145     527

  Fragments arranged by size: 

                 609     603     527     524     486     352     272     219
                 187     182     145      64
 
AvaI C'yCGr_G

Cuts at:      0    2316    4170 
   Size:       2316    1854
 
BamHI G'GATC_C

Cuts at:      0    1965    2474    4170 
   Size:       1965     509    1696

  Fragments arranged by size: 

                1965    1696     509
 
BanI G'GyrC_C

Cuts at:      0     193    1619    3959    4170 
   Size:        193    1426    2340     211

  Fragments arranged by size: 

                2340    1426     211     193
 
BanII G_rGCy'C

Cuts at:      0     506     981    3218    3843    4170 
   Size:        506     475    2237     625     327

  Fragments arranged by size: 

                2237     625     506     475     327
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     233    3547    3906    4170 
   Size:        233    3314     359     264

  Fragments arranged by size: 

                3314     359     264     233
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3086    4103    4170 
   Size:       3086    1017      67

  Fragments arranged by size: 

                3086    1017      67
 
BclI T'GATC_A

Cuts at:      0     381    4170 
   Size:        381    3789
 
BglII A'GATC_T

Cuts at:      0    1676    2886    3489    3537    4170 
   Size:       1676    1210     603      48     633

  Fragments arranged by size: 

                1676    1210     633     603      48
 
Bme1580I G_kGCm'C

Cuts at:      0     981    1719    2958    3218    4170 
   Size:        981     738    1239     260     952

  Fragments arranged by size: 

                1239     981     952     738     260
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     266    1526    3212    4170 
   Size:        266    1260    1686     958

  Fragments arranged by size: 

                1686    1260     958     266
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     907    1141    1445    4170 
   Size:        907     234     304    2725

  Fragments arranged by size: 

                2725     907     304     234
 
Bpu10I CC'TnA_GC

Cuts at:      0    1654    4170 
   Size:       1654    2516
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1337    3458    4170 
   Size:       1337    2121     712

  Fragments arranged by size: 

                2121    1337     712
 
BsaBI GATnn'nnATC

Cuts at:      0    1244    4170 
   Size:       1244    2926
 
BsaWI w'CCGG_w

Cuts at:      0     569    4170 
   Size:        569    3601
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     533     563    4170 
   Size:        533      30    3607

  Fragments arranged by size: 

                3607     533      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      75    2921    3805    3851    4170 
   Size:         75    2846     884      46     319

  Fragments arranged by size: 

                2846     884     319      75      46
 
BseYI C'CCAG_C

Cuts at:      0     505     980    1754    2133    4170 
   Size:        505     475     774     379    2037

  Fragments arranged by size: 

                2037     774     505     475     379
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76    1812    3091    3217    4170 
   Size:         76    1736    1279     126     953

  Fragments arranged by size: 

                1736    1279     953     126      76
 
BsiEI CG_ry'CG

Cuts at:      0    1642    4170 
   Size:       1642    2528
 
BsiHKAI G_wGCw'C

Cuts at:      0      41    1187    1719    1852    2275    3171    3815    4043 
   Size:         41    1146     532     133     423     896     644     228

Cuts at:   4043    4170 
   Size:        127

  Fragments arranged by size: 

                1146     896     644     532     423     228     133     127
                  41
 
BsmI GAATG_Cn'

Cuts at:      0     633     864    1090    1654    4170 
   Size:        633     231     226     564    2516

  Fragments arranged by size: 

                2516     633     564     231     226
 
Bsp1286I G_dGCh'C

Cuts at:      0      41     506     981    1187    1719    1852    2275    2958 
   Size:         41     465     475     206     532     133     423     683

Cuts at:   2958    3171    3218    3815    3843    4043    4170 
   Size:        213      47     597      28     200     127

  Fragments arranged by size: 

                 683     597     532     475     465     423     213     206
                 200     133     127      47      41      28
 
BspHI T'CATG_A

Cuts at:      0    1179    3389    4170 
   Size:       1179    2210     781

  Fragments arranged by size: 

                2210    1179     781
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     427    1380    2705    4170 
   Size:        427     953    1325    1465

  Fragments arranged by size: 

                1465    1325     953     427
 
BsrBI CCG'CTC

Cuts at:      0    1658    4170 
   Size:       1658    2512
 
BsrDI GCAATG_nn'

Cuts at:      0      72     628    2565    4170 
   Size:         72     556    1937    1605

  Fragments arranged by size: 

                1937    1605     556      72
 
BsrFI r'CCGG_y

Cuts at:      0     569    4170 
   Size:        569    3601
 
BsrGI T'GTAC_A

Cuts at:      0    2505    3708    4170 
   Size:       2505    1203     462

  Fragments arranged by size: 

                2505    1203     462
 
BssHII G'CGCG_C

Cuts at:      0    1231    4170 
   Size:       1231    2939
 
BssSI C'ACGA_G

Cuts at:      0      34    4170 
   Size:         34    4136
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1583    4170 
   Size:       1583    2587
 
BstBI TT'CG_AA

Cuts at:      0    3495    3507    4170 
   Size:       3495      12     663

  Fragments arranged by size: 

                3495     663      12
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3743    4170 
   Size:       3743     427
 
BstYI r'GATC_y

Cuts at:      0     919    1535    1676    1965    2220    2474    2886    3489 
   Size:        919     616     141     289     255     254     412     603

Cuts at:   3489    3537    4170 
   Size:         48     633

  Fragments arranged by size: 

                 919     633     616     603     412     289     255     254
                 141      48
 
BtgI C'CryG_G

Cuts at:      0     140    1929    4170 
   Size:        140    1789    2241

  Fragments arranged by size: 

                2241    1789     140
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2359    4170 
   Size:       2359    1811
 
BtsI GCAGTG_nn'

Cuts at:      0     474    1185    2901    3200    4170 
   Size:        474     711    1716     299     970

  Fragments arranged by size: 

                1716     970     711     474     299
 
ClaI AT'CG_AT

Cuts at:      0    1074    4170 
   Size:       1074    3096
 
DraI TTT'AAA

Cuts at:      0     218    1793    2096    4170 
   Size:        218    1575     303    2074

  Fragments arranged by size: 

                2074    1575     303     218
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     693    3090    4170 
   Size:        693    2397    1080

  Fragments arranged by size: 

                2397    1080     693
 
EaeI y'GGCC_r

Cuts at:      0    1926    4170 
   Size:       1926    2244
 
EarI CTCTTCn'nnn_

Cuts at:      0     494    3249    4170 
   Size:        494    2755     921

  Fragments arranged by size: 

                2755     921     494
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     907     934    1141    1445    2190    2621    2687 
   Size:        217     690      27     207     304     745     431      66

Cuts at:   2687    4170 
   Size:       1483

  Fragments arranged by size: 

                1483     745     690     431     304     217     207      66
                  27
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     235    4170 
   Size:        235    3935
 
EcoO109I rG'GnC_Cy

Cuts at:      0       6     416     977    4170 
   Size:          6     410     561    3193

  Fragments arranged by size: 

                3193     561     410       6
 
EcoRI G'AATT_C

Cuts at:      0    2055    2301    4170 
   Size:       2055     246    1869

  Fragments arranged by size: 

                2055    1869     246
 
EcoRV GAT'ATC

Cuts at:      0     641    4010    4170 
   Size:        641    3369     160

  Fragments arranged by size: 

                3369     641     160
 
HaeII r_GCGC'y

Cuts at:      0     536    4170 
   Size:        536    3634
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     448     480     533     565    1653    1685    3507    3539 
   Size:        448      32      53      32    1088      32    1822      32

Cuts at:   3539    3784    3816    4170 
   Size:        245      32     354

  Fragments arranged by size: 

                1822    1088     448     354     245      53      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     366     408    1110    1134    1281    2371    4170 
   Size:        366      42     702      24     147    1090    1799

  Fragments arranged by size: 

                1799    1090     702     366     147      42      24
 
MfeI C'AATT_G

Cuts at:      0    2072    4170 
   Size:       2072    2098
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     446    2269    2610    3272    3550    4170 
   Size:        446    1823     341     662     278     620

  Fragments arranged by size: 

                1823     662     620     446     341     278
 
MscI TGG'CCA

Cuts at:      0    1928    4170 
   Size:       1928    2242
 
MslI CAynn'nnrTG

Cuts at:      0      34     349     877    1866    2332    3239    3410    4170 
   Size:         34     315     528     989     466     907     171     760

  Fragments arranged by size: 

                 989     907     760     528     466     315     171      34
            
 
MspA1I CmG'CkG

Cuts at:      0      13     142    2424    3459    4170 
   Size:         13     129    2282    1035     711

  Fragments arranged by size: 

                2282    1035     711     129      13
 
NdeI CA'TA_TG

Cuts at:      0    1747    4170 
   Size:       1747    2423
 
NspI r_CATG'y

Cuts at:      0     433    2090    2369    3093    3205    3687    4170 
   Size:        433    1657     279     724     112     482     483

  Fragments arranged by size: 

                1657     724     483     482     433     279     112
 
PasI CC'CwG_GG

Cuts at:      0     537    4170 
   Size:        537    3633
 
PciI A'CATG_T

Cuts at:      0     429    2086    3089    3683    4170 
   Size:        429    1657    1003     594     487

  Fragments arranged by size: 

                1657    1003     594     487     429
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1546    4170 
   Size:       1546    2624
 
PfoI T'CCnGG_A

Cuts at:      0    1439    4003    4170 
   Size:       1439    2564     167

  Fragments arranged by size: 

                2564    1439     167
 
PpuMI rG'GwC_Cy

Cuts at:      0     416    4170 
   Size:        416    3754
 
PspOMI G'GGCC_C

Cuts at:      0     977    3214    4170 
   Size:        977    2237     956

  Fragments arranged by size: 

                2237     977     956
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1242    1274    4170 
   Size:       1242      32    2896

  Fragments arranged by size: 

                2896    1242      32
 
PstI C_TGCA'G

Cuts at:      0     304    1561    1579    4170 
   Size:        304    1257      18    2591

  Fragments arranged by size: 

                2591    1257     304      18
 
PvuI CG_AT'CG

Cuts at:      0    1642    4170 
   Size:       1642    2528
 
PvuII CAG'CTG

Cuts at:      0    2424    4170 
   Size:       2424    1746
 
SacII CC_GC'GG

Cuts at:      0     143    4170 
   Size:        143    4027
 
SalI G'TCGA_C

Cuts at:      0     364    4170 
   Size:        364    3806
 
SapI GCTCTTCn'nnn_

Cuts at:      0     494    4170 
   Size:        494    3676
 
SfcI C'TryA_G

Cuts at:      0     300    1557    1575    2975    2999    3702    4170 
   Size:        300    1257      18    1400      24     703     468

  Fragments arranged by size: 

                1400    1257     703     468     300      24      18
 
SpeI A'CTAG_T

Cuts at:      0    3106    4170 
   Size:       3106    1064
 
StyI C'CwwG_G

Cuts at:      0      50     189    1812    2757    4170 
   Size:         50     139    1623     945    1413

  Fragments arranged by size: 

                1623    1413     945     139      50
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     165     290    3391    4170 
   Size:        165     125    3101     779

  Fragments arranged by size: 

                3101     779     165     125
 
TatI w'GTAC_w

Cuts at:      0     248    1948    2089    2505    2568    2922    3708    4170 
   Size:        248    1700     141     416      63     354     786     462

  Fragments arranged by size: 

                1700     786     462     416     354     248     141      63
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      14      46    1863    1895    4170 
   Size:         14      32    1817      32    2275

  Fragments arranged by size: 

                2275    1817      32      32      14
 
XbaI T'CTAG_A

Cuts at:      0     804    2059    4170 
   Size:        804    1255    2111

  Fragments arranged by size: 

                2111    1255     804
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    4154    4170 
   Size:       4154      16
 
XmnI GAAnn'nnTTC

Cuts at:      0     230    2903    4170 
   Size:        230    2673    1267

  Fragments arranged by size: 

                2673    1267     230
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AgeI     AhdI     AlfI    AlwNI     ApaI
    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbsI    BciVI
     BclI    BglII Bme1580I     BmrI     BpmI   Bpu10I     BsaI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI     DraI     DrdI     EaeI     EarI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV    HaeII    Hin4I   HincII     MfeI     MmeI     MscI
     MslI   MspA1I     NdeI     NspI     PasI     PciI    PflMI     PfoI
    PpuMI   PspOMI     PsrI     PstI     PvuI    PvuII    SacII     SalI
     SapI     SfcI     SpeI     StyI    TaqII     TatI     TstI     XbaI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AleI     AloI
     AscI     AseI    AsiSI    AvrII     BaeI     BbeI    BbvCI     BcgI
    BfrBI     BglI     BlpI    BmgBI     BmtI     BplI    BpuEI    BsaAI
    BsaHI    BsiWI    BsmBI    BspEI   BstEII  BstZ17I   Bsu36I    CspCI
   DraIII     EagI     EciI  EcoICRI     FalI     FseI     FspI    FspAI
  HindIII     HpaI     KasI     KpnI     MluI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI    RsrII     SacI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XhoI
     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com